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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... coli O111:B4 (Catalog# L2630-10MG, lot 114M4009V) were purchased from Sigma (St. Louis, MO). Budesonide (Catalog# S1286) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli wild type (wt) Hfq protein and its mutants were expressed from pET15b vector (Novagen) and purified as described [38] ...
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bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
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bioRxiv - Molecular Biology 2022Quote: ... coli HMS174 (DE3) (F- recA1 hsdR(rK12- mK12+) Rif R (DE3)) was purchased from Novagen. E ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... as previously performed,24 γ-irradiated LPS from Escherichia coli O111:B4 (BioXtra; Sigma-Aldrich) dissolved in PBS was IV-injected at the dose of 2.4 ng ...
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bioRxiv - Molecular Biology 2020Quote: ... coli ProQ protein or its N-terminal domain (NTD) were cloned into pET15b vector (Novagen) using BamHI restriction site (Supplemental Table S2) ...
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bioRxiv - Microbiology 2021Quote: ... coli membrane fractions were determined using a bicinchoninic acid (65) protein assay kit (Sigma-Aldrich) with BSA (Interchim ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells by the heat-shock approach as described in the supplier’s manual (Novagen/Agilent) and by antibiotic selection using Zeocin (Invitrogen/ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: RIG-I was expressed using pET28 SUMO vector in Escherichia coli strain Rosetta (DE3) (Novagen). Soluble cell lysate was purified using a Ni2+-nitrilotriacetate (QIAGEN ...
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bioRxiv - Immunology 2020Quote: ... To stimulate cells 0.3 ng* μl−1 LPS (Lipopolysaccharide from Escherichia coli 0111:B4, Sigma) was added and cells were incubated for 4 hours at 37°C and 7 % CO2 ...
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bioRxiv - Microbiology 2021Quote: ... The BMDMs were primed with 100 ng/mL O26:B6 Escherichia coli LPS (Sigma®) and incubated overnight at 37°C with 5% CO2.
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bioRxiv - Biochemistry 2022Quote: ... The cloned vector was transformed into Escherichia coli BL21 HMS174(DE3) chemically competent cells (Novagen). A colony was picked and placed into an overnight outgrowth ...
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bioRxiv - Neuroscience 2022Quote: ... anti-adenomatous polyposis coli clone CC1 (APC/CC-1) (1:100, mouse, Millipore Cat# OP80) anti-platelet derived growth factor receptor alpha (PDGFRa ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli C wildtype and phage resistant strains were diluted 10 times in Ringer’s solution (Sigma). 50 µL of each 10 times diluted overnight culture (5 x 106 cells ...
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bioRxiv - Neuroscience 2024Quote: ... we used activated microglia induced by lipopolysaccharides (LPS, Cat# Escherichia coli O111:B4, Sigma-Aldrich) in this study ...
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Antimicrobial and antibiofilm activity of human recombinant H1 histones against bacterial infectionsbioRxiv - Microbiology 2024Quote: Histones H1.2 was cloned into pET21a and expressed in Escherichia coli BL21(DE3) (Merk Millipore) while H1.0 and its C-terminal domain (CTD ...
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bioRxiv - Cell Biology 2023Quote: Human αSN cDNA was transformed into Escherichia coli Strain BL21 (DE3) (Novagen, Madison, Wis., USA) using the pNIC28-Bsa4 plasmid ...
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bioRxiv - Biophysics 2023Quote: Chaperonin 60 lyophilized powder (GroEL, 1 mg) from Escherichia coli was purchased from Sigma-Aldrich (Cat # C7688 ...
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bioRxiv - Immunology 2023Quote: ... Lipopolysaccharides (LPS) Escherichia coli O111:B4 origin (L2630) and Evans blue (E2129) procured from Sigma, USA ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli cells were counter-selected using Thiosulfate citrate bile salts sucrose (TCBS, Sigma-Aldrich) agar ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... followed by stimulation with LPS from Escherichia coli 055: B5 (20 µg/ml, Sigma-Aldrich) for 3 days in complete RPMI medium (L-glutamine ...
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bioRxiv - Microbiology 2024Quote: ... The BMDMs were primed with 100 ng/mL O26:B6 Escherichia coli LPS (Sigma®) and incubated overnight at 37°C with 5% CO2.
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bioRxiv - Immunology 2024Quote: LPS from Escherichia coli O111:B4 was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dexamethasone (Dx ...
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bioRxiv - Immunology 2024Quote: ... the cells were treated with 10ng/ml LPS (Lipopolysaccharides from Escherichia coli O55:B5; Sigma). After 3 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... were expressed in Escherichia coli BL21 (DE3) slyD- cells [56] carrying the plasmid pRARE2 (Novagen). Recombinant GST (vector pGEX-4T2 (Amersham) ...
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bioRxiv - Neuroscience 2024Quote: ... lipopolysaccharides (LPS) from Escherichia coli O26:B6 (Sigma-Aldrich; 10, 100, and 1000 μg/mL), and recombinant TNF-α (Peptrotech;10 ...
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bioRxiv - Neuroscience 2021Quote: ... and then supplemented with 2% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Sigma Aldrich 410896) as the UV initiator ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Plant Biology 2019Quote: ... The transformants were then spotted on SD (-Trp -Leu -His) selection media containing 0.5/1mM 3-Amino-1,2,4-triazole (3-AT; Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated for 4 hours with anti-His hexahistidine antisera conjugated to horseradish peroxidase (HRP) (Sigma) diluted in TBST at a ratio of 1:5000 ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were cleaved by incubation with 1.5 % (w/w) His-tagged HRV-3C protease (Millipore Sigma SAE0045) or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067 ...
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bioRxiv - Biochemistry 2021Quote: A C-terminal 10x His-tagged Synechocystis PCC 6803 ocp gene (slr1963) was cloned in a pCDFDuet (Novagen), resulting in a plasmid named pEP4 ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma] ...
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bioRxiv - Bioengineering 2021Quote: ... aliquots of each fraction were visualized via immunoblotting (anti-His antibody; Cat: SAB2702218, Sigma Aldrich Canada, Oakville, Canada) to identify fractions enriched for RBD ...
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bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak corresponding to mono-SPY-His-tagged streptavidin tetramer was collected and concentrated using Amicon 10K (Millipore). The concentrated mono-SPY-His6-tagged streptavidin tetramer was further purified through Hiload superdex75 (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... His-tagged GA-MatryoshCaMP6s were eluted in 1.5 mL 250 mM imidazole (Sigma-Aldrich; CAS 1467-16-9) 20 mM MOPS pH 7.0 ...
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bioRxiv - Microbiology 2023Quote: ... The fractions containing His-SSRP1 were concentrated with an Amicon centrifugal filter unit (50 kDa cut-off, Millipore) and buffer exchanged into storage buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the His-tagged nucleotidases were pooled and concentrated using an Amicon 10 kDa MWCO filter (Millipore). The protein was buffer-exchanged into gel filtration buffer (100 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... and lysates were clarified by ultracentrifugation (20,000 x g for 20 min) and incubated with 0.4 mL of His-Select nickel resin (Sigma) for 1 h at 4°C with gentle mixing ...
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bioRxiv - Biochemistry 2024Quote: ... and the soluble fraction was purified by affinity chromatography (either Ni2+ or Strep) (His-Select resin (Sigma-Aldrich); Strep-Tactin®XT 4Flow resin (IBA Lifesciences)) ...
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bioRxiv - Biochemistry 2024Quote: Cleaved MntR was loaded onto a 5 ml column of Ni-NTA His•Bind® Resin (Millipore Sigma) equilibrated with Buffer A and then eluted with 40 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... EIF4E2 shRNA#2 (sh4EHP#2) (Sigma, TRCN0000280916) and EIF4E2 shRNA#3 (sh4EHP#3 ...