Labshake search
Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... coli DH5⍺ was grown in 25 mL Luria-Bertani media (Sigma-Aldrich) at 37 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli cells was resuspended in 50.0 μL of 1X TE (Sigma-Aldrich). The cells were lysed and solubilised with 2000 U of Ready-Lyse Lysozyme (Epicentre ...
-
bioRxiv - Biochemistry 2023Quote: ... coli was cloned into the first multiple cloning site of pRSFDuet (Novagen) with an N-terminal glutathione S-transferase (GST ...
-
bioRxiv - Genetics 2023Quote: ... the Cas9 protein was expressed in BL21 Rosetta Escherichia coli strain (Novagen) in magic medium at 37 °C for 12 h followed by 24 h at 18 °C ...
-
bioRxiv - Bioengineering 2023Quote: ... coli 0111:B4 (LPS) was purchased from Sigma-Aldrich (St. Louis, MO). Raw Blue™ cells ...
-
bioRxiv - Biochemistry 2023Quote: ... coli GyrA coding sequence was cloned in pACYC-Duet (from Millipore/Novagen) under the T7 promoter ...
-
bioRxiv - Cell Biology 2024Quote: ... 50 ng/ml of LPS from Escherichia coli O26:B6 (Sigma-Aldrich) was added to appropriate wells for additional 2 h or 24 h ...
-
bioRxiv - Molecular Biology 2023Quote: LPS (n=10): Lipopolysaccharide (Escherichia coli O55:B5, L2880-10MG, Sigma-Aldrich) was solubilized in sterile normal saline and animals were intraperitoneally (IP ...
-
bioRxiv - Biochemistry 2023Quote: ... coli GyrA coding sequence was cloned in pACYC-Duet (from Millipore/Novagen) under the T7 promoter ...
-
bioRxiv - Genetics 2024Quote: ... coli OP50 were spotted with 25µL 1mg/mL thapsigargin in DMSO (Sigma) or DMSO only directly on to the E ...
-
bioRxiv - Plant Biology 2024Quote: ... MBP fusion proteins were expressed in Escherichia coli Rosetta (DE3) pLysS (Novagen) and purified using Amylose Resin (New England Biolabs ...
-
bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
-
bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
-
bioRxiv - Microbiology 2020Quote: ... anti-mouse (BioSupplies) or anti-His (CBM) secondary antibodies conjugated to alkaline phosphatase (Sigma Aldrich) as described in Pedersen et al35 ...
-
bioRxiv - Bioengineering 2019Quote: Ni-NTA columns were prepared by packing 1 mL Ni-NTA His•Bind resins (Sigma) into a 6-ml ISOLUTE® Single Fritted Reservoir column with 10 μm polyethylene frit (Biotage ...
-
bioRxiv - Bioengineering 2020Quote: ... His tagged FLIPsuc sensors were purified by affinity chromatography using Ni-NTA bead material (Novagen). Binding to the resin was performed in batch at 4 °C for 4 h ...
-
bioRxiv - Biophysics 2020Quote: ... or 8 μM His-tagged NSP2 labelled with 8 μM Atto488-nitrilotriacetic acid (NTA, Sigma), and buffer (0.5 × phosphate saline buffer ...
-
bioRxiv - Microbiology 2021Quote: ... PfArlH or biotinylated PfArlI were subsequently mixed with HIS-Select® Nickel Affinity resin (SIGMA) while lysate of cells overexpressing Strep-SaArlI was mixed with Streptactin column material (IBA GmbH ...
-
bioRxiv - Biochemistry 2021Quote: ... DTT was added to a final concentration of 0.5 mM and His-SUMO protease (Sigma) was added to a concentration 100-fold lower than the total protein concentration ...
-
bioRxiv - Bioengineering 2020Quote: ... The eluted His-tagged RBD was then incubated with biotin-tagged HRV C3 protease (Sigma) and passed through a streptavidin-agarose column to deplete the protease and the 8XHis-SBP peptide ...
-
bioRxiv - Microbiology 2020Quote: ... supernatants were subjected to purification using either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare) ...
-
bioRxiv - Plant Biology 2020Quote: ... and purified with a commercial kit according to manufacturer’s instructions (His-bind buffer kit, Millipore).
-
bioRxiv - Cell Biology 2021Quote: ... and a western blot was performed using anti-HIS (Sigma H-1029, mouse, 1:3000) and anti-HA (Biolegend MMS-101R or ...
-
bioRxiv - Molecular Biology 2024Quote: ... His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen®). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic® ...
-
bioRxiv - Microbiology 2024Quote: ... The cell lysate was then incubated with HIS-select HF Nickel Affinity Gel (Millipore-Sigma) overnight ...
-
bioRxiv - Microbiology 2024Quote: ... The cell lysate was then incubated with HIS-select HF Nickel Affinity Gel (Millipore-Sigma) overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 1 µg/mL His-EqtII-EGFP and DAPI (D9542, SIGMA) at room temperature for 1 h ...
-
bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM NaPO4 ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM HEPES ...
-
bioRxiv - Plant Biology 2023Quote: ... For super-shift assays monoclonal 0.2 μl of Anti-His antibody (H1029, Sigma-Aldrich, USA) was used ...
-
bioRxiv - Plant Biology 2023Quote: ... with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore) following manufacturer’s recommendations.
-
bioRxiv - Biochemistry 2023Quote: ... The eluted His-tagged σNS was concentrated using a 10-kDa centrifugal filter unit (Millipore), dialyzed into 50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Biochemistry 2022Quote: ... and the supernatant was purified by affinity chromatography using Ni-NTA His-bind resin (Novagen). The column packed with the resin was equilibrated with binding buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% v/v heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich, F7524), Penicillin-Streptomycin 100U/ml (Sigma Aldrich ...
-
bioRxiv - Plant Biology 2023Quote: ... pH 7.4 and then bound to poly-prep chromatography columns containing His-bind resin (Novagen). The yeast lysate mixture was loaded in the columns ...
-
bioRxiv - Biochemistry 2023Quote: ... and His-AC elution buffer were supplemented with 0.1 v/v% Tween-20 (Sigma Aldrich), 0.1 v/v% NP-40 (Thermo Fischer Scientific) ...
-
bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated for 1 hour with HIS-Select® Nickel Affinity Gel (Sigma), 300 μL of gel per 500 mL of His6-SUMO-AGARP expression cell culture ...
-
bioRxiv - Bioengineering 2020Quote: ... hematoxylin and eosin (H&E) staining was performed using Harris hematoxylin solution (Sigma-Aldrich) and eosin solution (Electron Microscopy Sciences) ...
-
bioRxiv - Cell Biology 2021Quote: ... Slides were stained with Hematoxylin and Eosin (H&E) and Goldner’s trichrome (Sigma-Aldrich). Images were obtained with Lionheart LX (Biotek Instruments Inc ...
-
bioRxiv - Microbiology 2021Quote: ... substrate and the positive control E-64 (Sigma Aldrich, St. Louis, MO, Unites States) were prepared at 10 mM in DMSO ...
-
bioRxiv - Neuroscience 2020Quote: ... The mixed glial cells were plated on poly-D-lysine (PDL, #A003-E, Millipore) coated 10 cm dishes and cultured in DMEM medium (#1196092 ...
-
bioRxiv - Immunology 2021Quote: ... then rehydrated and stained with hemoxtylin & eosin (H&E) (Sigma-Aldrich, St. Louis, MO).
-
bioRxiv - Pathology 2020Quote: Lungs were sectioned and stained with both Hematoxylin/Eosin (H&E) (Sigma Aldrich, USA) and Masson Trichrome (Abcam ...
-
bioRxiv - Developmental Biology 2019Quote: ... B16 Melanoma cells or R1/E ESCs were fixed with 10% formalin (Sigma; HT501128) and incubated at 370C for 10 minutes ...
-
bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
-
bioRxiv - Neuroscience 2021Quote: ... Cells were plated in poly-D-lysine (50 μg/ml, Millipore A-003-E) coated flasks and grown in astrocyte growth media containing DMEM (Gibco 11960044) ...
-
bioRxiv - Biochemistry 2021Quote: ... coverslips were coated with 0.05 mg/ml Phaseolus vulgaris erythroagglutinin (PHA-E) lectin (Sigma) and incubated in a humid environment for 15 min at 37°C ...
-
bioRxiv - Physiology 2021Quote: ... The basal media utilized in all conditions consisted of Williams E medium (Sigma #W1878), PenStrep ...
-
bioRxiv - Bioengineering 2019Quote: ... sericata maggot E/S was assessed using a Protease Fluorescent Detection Kit (Sigma Aldrich), This kit detects the activity of serine ...