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Citations for Millipore Sigma :
1751 - 1800 of 2155 citations for Cholera Toxin Subunit B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-vinculin (1:1000;ThermoFisher Sientific mouse anti-α-tubulin (clone B-512; 1:5000; Sigma-Aldrich) were used as primary antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... 2012) were selected with 2 μg/ml hygromycin B (Duchefa) or 15 μg/ml PPT (phosphinothricin, Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2022Quote: ... the fixed and permeabilized cell monolayers were labeled with Phalloidin-Tetramethyl rhodamine B (Sigma-Aldrich, St. Louis, MO) for 1h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... Ethyleneglycol-bis-(b-amino ethyl ether) N,N,N’,N’-tetraacetic acid (EGTA, Sigma-Aldrich St. Louis, MO) was added to a final concentration of 2.5 mM and samples were incubated at 37°C for an additional 15 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Normalization was performed by fixing the cells using sulforhodamine B dye (Sigma-Aldrich, Co., St. Louis, MO, USA) as described before (Chen et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were carried out using the indicated antibodies: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), BLM (Bethyl ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in the presence or absence of aphidicolin 2μM for 3 hours together with cytochalasin B (3μg/ml, Merck Millipore) to block cytokinesis prior to fixation for immunofluorescence.
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bioRxiv - Cell Biology 2023Quote: Salivary glands were dissected at 3rd instar larval stage in PBS and treated with 0.1% Leptomycin B (Sigma) in 10 min before fixation in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Mice were orally gavaged with 100 µl sterile solution of 10 mg/ml rhodamine B dextran (Sigma R9379) in 2% methylcellulose (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Bioengineering 2023Quote: ... The immobilized Candida antarctica lipase B (CALB) supported on acrylic resin (Novozym 435) was obtained from Sigma Aldrich. mPEG2k-NH2 was purchased from Bide Pharmatech (Shanghai ...
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bioRxiv - Genomics 2023Quote: ... C57BL/6J × CAST/EiJ (B6xCAST) mESCs were cultured on plates coated with 0.1% gelatin (EMD Millipore ES006-B) in 80/20 medium comprising 80% 2i medium and 20% mESC medium ...
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bioRxiv - Genomics 2023Quote: ... Slides were then placed in equal parts Weigert’s Iron Hematoxylin Solution A: Solution B (Cat No. HT1079, Sigma-
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bioRxiv - Bioengineering 2023Quote: ... b-mercaptoethanol and 1000 U ml-1 LIF) and 2 ug ml-1 doxycycline (cat. 33429 Sigma-Aldrich). MEFs were reprogrammed in the presence of doxycycline for 12 days and without doxycycline for the last 5 days ...
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bioRxiv - Bioengineering 2022Quote: ... A to B chromogens) was used to visualize stains by incubating for 10 min at 25 °C (Millipore). Samples were mounted in an aqueous-based mounting solution and sealed with clear nail polish ...
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bioRxiv - Cancer Biology 2022Quote: ... 4°C) and resuspended in B cell medium supplemented with 8 μg/mL polybrene (#TR-1003, EMD Millipore) at a density of 2×106 cells/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... an antibiotic (100 U/mL Penicillin and 0.1 mg/mL Streptomycin) and antimycotic (0.25 μg/mL Amphotericin B) solution from Sigma. Cell lines were maintained at 37°C with 5% CO2 atmosphere ...
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bioRxiv - Immunology 2023Quote: ... Enriched B cells were spun down and resuspended in PBMC medium supplemented with 80 μg/mL apotransferrin (Sigma) at 106 cells/mL ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS and 50 μg/ml Lipopolysaccharide neutralizing agent Polymyxin B sulfate (cat # 1405-20-5; Sigma-Aldrich) for 30 min at 37°C while shaking by 750 rpm on a heating block ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were plated on 0.4% gelatin-coated 60 mm culture dishes (Gelatin Type B Powder, Sigma-Aldrich, G9391 ...
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bioRxiv - Genomics 2023Quote: ... (b) Reduce background signal by pre-clearing with 20µL of Protein A/G magnetic beads (Millipore 16-663) per 1mL of sample for 2 hours at 4°C with rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody65 (kind gift of G ...
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bioRxiv - Immunology 2023Quote: Spontaneous antibody secretion from IgA-switched B cell populations was assessed in MultiScreen 96-well filter plates (Millipore) that were coated with 2 µg/ml goat anti-human Ig (Southern Biotech ...
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bioRxiv - Physiology 2023Quote: ... and then moved into 6-well-plates containing digestion medium (DMEM + 10% FBS + 1% amphotericin B (A2942, Sigma)) and 10% liberase (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... trabectedin was thawed and formulated in 0.1 g of Hydroxy B-Cyclodextrin (Sigma-Aldrich, St. Louis, MO, MKBL6868V) and sterile PBS at 0.0533 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... and 1.5 ml of serum-free DMEM containing polymyxin B (30 µg/mL, Sigma-Aldrich, catalog number P4932) was added to each well ...
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bioRxiv - Neuroscience 2023Quote: ... and infiltrated with hard Durcupan resin (11.4 g reagent A, 10 g B, 0.3 g C, and 0.1 g D; all Sigma-Aldrich) at 2:1 ...
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bioRxiv - Microbiology 2023Quote: ... A and B groups were orally inoculated with 0.2 ml 15 mg ml-1 ampicillin (Sigma-Aldrich, USA) twice daily to disorder the gut microbiota ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 µL of solution B (0.5 mg/mL of Trypsin inhibitor, Sigma T9253, 0.1 mg/mL RNAse Type A, Sigma R4875 ...
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bioRxiv - Neuroscience 2024Quote: ... the culture plate were coated with 0.5 mg/mL of poly-L-lysine (PLL) in B-buffer (3.1 mg/mL boric acid and 4.75 mg/mL borax, Sigma) at 37°C and 5% CO2 for 4 hours ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Peptides were separated using a 250 min gradient ranging from 2 % to 90 % mobile phase B (mobile phase A: 2% acetonitrile [Scharlab S.L.], 0.1 % formic acid [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2020Quote: ... The transformed cells at 25°C (OD600 = 0.6) were induced with 0.5 mM isopropyl b-D-thiogalactopyranoside (IPTG; Sigma, USA), which resulted in the expression of the maltose-binding protein (MBP)-tagged NlGr7 protein ...
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bioRxiv - Cell Biology 2019Quote: ... GST (clone 8-326, MA4-004 from Thermo Fischer, 1:1000) and Cyclin B (C8831 from Sigma, 1:1000). The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad ...
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bioRxiv - Biophysics 2019Quote: ... the media was refreshed with PB-MAX™ media supplemented with cytochalasin B (Sigma-Aldrich LLC, St. Louis, MO) at a final concentration of 6 μg/mL to block cytokinesis ...
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bioRxiv - Systems Biology 2020Quote: Recombinant HA-tagged Gln4 protein for use as a standard was produced by transforming E.coli strain Rosetta-gami B (DE3; Novagen) with pET19b-GLN4 ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used in this work were: mouse anti-α-tubulin clone B-512 at 1:1500 (Sigma Aldrich), mouse anti-EB1 at 1:500 (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... blotted and immunodetected with a rat anti-CLASP2 or mouse anti-α-tubulin antibodies (clone B-512, Sigma Aldrich). For analysis of recombinant CLASP2 C-term ...
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bioRxiv - Cancer Biology 2020Quote: ... LPS stimulated B-cells were transduced by retroviral pool spin infection in 8 μg/μl of polybrene (Sigma-Aldrich). Each retroviral pool contained vectors expressing ...
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bioRxiv - Immunology 2019Quote: ... the proteins samples were incubated with 50 μg/mL of polymyxin B sulphate salt (Sigma-Aldrich, St. Louis, MO) for 30 min at 37 °C to remove possible residual LPS.
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bioRxiv - Biochemistry 2020Quote: ... Buffer B contained during protease cleavage 300 mM NaCl and 0.1% (v/v) CHAPS (Sigma-Aldrich, St Louis, MI). The HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... cells were stimulated with HDM or the known AhR agonist FICZ (5,11-Dihydroindolo[3,2-b]carbazole-6-carboxaldehyde, Tocris Bioscience, Bristol, UK or Sigma-Aldrich) to activate the AhR and induce CYP1A1 transcription ...
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bioRxiv - Synthetic Biology 2021Quote: A17iCre mESCs (49) were cultured on plastic tissue culture plates coated with 0.1% gelatin (EMD Millipore ES-006-B) at 37°C and 5% CO2 in ‘80/20’ medium comprising 80% 2i medium and 20% mESC medium ...
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bioRxiv - Bioengineering 2020Quote: ... All cells were cultured on tissue culture polystyrene dishes treated with 0.1% v/v gelatin solution (ES-006-B, Millipore) and were incubated in a humidified incubator at 37°C and 5% CO2 until they reached confluence ...
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bioRxiv - Neuroscience 2021Quote: ... Astrocytes were derived from IPSCs by culturing progenitors in DMEM/F12 supplemented with N2 growth factor and 0.5% FBS (Cat# ES-009-B, Millipore) for 14-20 days ...
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bioRxiv - Bioengineering 2021Quote: ... The LCLs were maintained under aseptic conditions in a 1:1 mixture of Dulbecco’s modified Eagle medium and nutrient mixture F-12 (DMEM/F12; DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Microbiology 2020Quote: ... Transformants were selected in medium lacking uridine or uracil or in the presence of 150 μg/ml hygromycin B (Sangon) or 0.1 μg/ml pyrithiamine (Sigma). For the recycling usage of the selectable marker pyr4 ...
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bioRxiv - Microbiology 2021Quote: ... livers were finely minced with a sterile razor blade and digested overnight at 37°C in 40 mL 1×PBS containing 2% penicillin/streptomycin/amphotericin B (1×PBS −2% PSF) and 5 mL 0.5% clostridial collagenase solution (0.025 g of collagenase (Sigma, C0130) in 5 mL of dH20 for immediate use) ...
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bioRxiv - Neuroscience 2020Quote: ... The human brain slices were subjected to immunofluorescence staining and the autofluorescence signal was blocked by 0.1% (w/v) Sudan Black B (199664, Sigma) in 70% ethanol ...
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bioRxiv - Microbiology 2021Quote: ... Overnight cultures were diluted 1:100 in prewarmed LB broth containing 0.4 mM IPTG and 20 µg mL-1 polymyxin B sulfate (Sigma). Samples were incubated at 37°C ...
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bioRxiv - Physiology 2021Quote: ... For measuring [K+] they were filled at the tips with Potassium Ionophore I-Cocktail B (5% valinomycin; Sigma-Aldrich) and back-filled with 500 mM KCl (Rodgers et al. ...