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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and Tissue Protein Extraction Reagent (Pierce, Waltham, MA, USA) supplemented with 2.5% of 2-mercaptoethanol (Sigma Aldrich, Darmstadt, Germany). Cell lysates were boiled for 8 min and stored at − 80°C ...
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bioRxiv - Biochemistry 2023Quote: 50 µg of refolded TREM2 protein in 100 mM ammonium bicarbonate buffer was digested with 2 µg trypsin (Sigma) at 37 °C for overnight ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were harvested between 24 and 48 h post-transfection and lysed with M-PER Mammalian Protein Extraction Reagent (Pierce Biotechnology) containing a mammalian protease inhibitor cocktail (SIGMA P8340, Sigma-Aldrich) according to the protocol of the manufacturer ...
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bioRxiv - Cancer Biology 2019Quote: CAF cell monolayers were lysed for protein with RIPA buffer containing protease inhibitor cocktail at 1:100 dilution (both Sigma-Aldrich, Dorset, UK). Lysed cell suspensions were quantified for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was normalized for all tissues with RIPA buffer and 2x SDS sample buffer containing β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO) was added ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer containing 25 mM Tris ...
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bioRxiv - Bioengineering 2020Quote: ... endothelial cells were enzymatically removed and subsequently epithelial cells were lysed with a protein lysis buffer (MSD, R60TX-3) containing protease (ThermoFischer, 78425) and phosphatase inhibitors (Sigma-Aldrich, P0044 & P5726). Protein extraction was performed according to standard protocols and the quantification of the total amount of protein/ sample was done by Bradford assay (Thermo Fischer ...
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bioRxiv - Immunology 2022Quote: All protein was extracted from ear skin samples by homogenizing tissues in RIPA buffer containing protease inhibitor cocktails (Sigma-Aldrich, St. Louis, MO). SCF levels were determined using ELISA kit (R&D Systems) ...
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bioRxiv - Plant Biology 2023Quote: ... were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™, Mini, EDTA-free, Sigma-Aldrich, 04693159001). Proteins were eluted from magnetic beads by boiling at 95°C for 10 min in 50 µl 2x Laemmli sample buffer ...
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bioRxiv - Genetics 2021Quote: The mobilization of carbohydrate from germinating kernels was investigated by assaying root growth on large plates (245 x 245 x 25mm) containing ½ strength MS media and 2% phytagel (Sigma Aldrich #P8169), pH buffered to 5.7 with or without the addition of 2% Suc ...
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bioRxiv - Cell Biology 2022Quote: ... around 150 young adults were transferred to fresh NGM plates containing 49.5 μM 5-fluoro-2’-deoxyuridine (FUDR, Sigma-Aldrich, MO, USA) to prevent progeny production ...
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bioRxiv - Developmental Biology 2019Quote: ... the embryos used for WISH or confocal analysis were cultured in fish water containing 0.003% 1-phenyl-2-thiouera (PTU, Sigma-Aldrich, cat. # P7629) from 24hpf on ...
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bioRxiv - Cancer Biology 2020Quote: ... Pre-treated cells were incubated with media containing 20 μM 5-chloro-2’-deoxyuridine (CldU) (Milipore Sigma; Cat# C6891, St. Louis, MO) for 30 m at 37°C before being washed and incubated in media containing 100 μM 5-iodo-2’-deoxyuridine (IdU ...
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bioRxiv - Cell Biology 2021Quote: ... age-synchronized animals at the L4 stage were collected in M9 buffer and plated on NGM plates containing 5-fluoro-2’-deoxyuridine (FUDR, Sigma, 50 μM) to prevent egg-laying (84).
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bioRxiv - Neuroscience 2020Quote: ... and postfixed for 24h in a solution containing 2% paraformaldehyde, 2.5% glutaraldehyde (TAAB, G002, UK) and 0.003% CaCl2 (Sigma, C-2661-500G, Germany) in sodium cacodylate buffer (0.1M) ...
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bioRxiv - Neuroscience 2021Quote: EC sections were postfixed for 24h in a solution containing 2% paraformaldehyde, 2.5% glutaraldehyde (TAAB, G002, UK) and 0.003% CaCl2 (Sigma, C-2661-500G, Germany) in sodium cacodylate (Sigma ...
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bioRxiv - Neuroscience 2021Quote: Sections containing BA21 were selected and postfixed for 24h in a solution containing 2% paraformaldehyde, 2.5% glutaraldehyde (TAAB, G002, UK) and 0.003% CaCl2 (Sigma, C-2661-500G, Germany) in sodium cacodylate (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were transfected with 100 μL of transfection buffer containing 2 μL of X-tremeGENE™ HP DNA transfection reagent (Sigma, 6366244001), 2 μL of plasmid DNA (tdTomato-Lifeact-7 ...
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bioRxiv - Microbiology 2019Quote: ... after 3 days of growth membranes/biofilms were transferred to fresh agar wells containing 20uL of DNase 1 (2 Kunitz units / µL) (Sigma-Aldrich #D4263) spotted directly underneath the biofilm ...
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bioRxiv - Biophysics 2020Quote: ... oocytes were incubated twice (1st 45 min, 2nd variable) in OR-2 containing 3 mg/ml collagenase type IV (Sigma, C-9891). Defolliculated oocytes were stored in ND96 solution containing (in mM) ...
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bioRxiv - Immunology 2022Quote: ... Cells were subsequently washed with Perm/Wash and FACS buffer and fixed in PBS containing 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Immunology 2022Quote: ... Cells were washed again with Perm/Wash and FACS Buffer and fixed in PBS containing 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO). For each sample ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed once with a lovastatin-free medium and then grown in a medium containing 2 mM (±) mevalonolactone (Sigma-Aldrich, M4667) with or without 1 µM 5-Ph-IAA ...
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bioRxiv - Bioengineering 2022Quote: ... 1993) containing binary expression vectors of interest (Online Resource 2) was cultured on LBA (LB Agar, Sigma-Aldrich, St. Louis, MO, USA) supplemented with appropriate antibiotics and incubated at 28°C for two days.
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... tumors were cut into small pieces and digested for 1.5 hours at 37 °C in 5 ml of DMEM/F12 containing 2 mg/ml of collagenase type I (WAKO, no. 037-17603) and 100 U/ml of hyaluronidase (Sigma, no. H3506). Following digestion ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... HEK293T cells were seeded in a 6-well plate so that they were 70-90% confluent the next day (∼0.8x10^6 - 1x10^6 cells/well in 2 mL of standard growth media containing 10% FBS (Millipore, TMS-013-B) and 1x Penicillin-Streptomycin (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: EC sections were post-fixed for 24h in a solution containing 2% paraformaldehyde, 2.5% glutaraldehyde (TAAB, G002, UK) and 0.003% CaCl2 (Sigma, C-2661-500G, Germany) in sodium cacodylate (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... Sections were stained for 2 h in PBS containing 2 µg/ml DAPI and mounted in a 1:1 mix of glycerol and water containing 1 % DABCO antifade (Sigma-Aldrich, USA) for confocal imaging using a Zeiss (Cambridge ...
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bioRxiv - Cancer Biology 2019Quote: ... containing 1% glucose, and protease inhibitors (0.1 mM phenylmethanesulfonyl fluoride, 2 μM E-64 and 10μM leupeptin) (Sigma-Aldrich, St. Louis, Missouri) at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Isolated CP colonies (1-2 colonies) were sub-cultured in 50 mL tubes containing thioglycolate broth with resazurine (Sigma-Aldrich®, MO) supplemented with 2% beef extract (Bioworld ...
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bioRxiv - Biochemistry 2022Quote: The age synchronized (by picking the L4 stage hermaphrodites) worms were placed into 60 mm NGM agar plates containing 2 mM paraquat (Sigma, Cat. #: 856177) in adult day 1 ...
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bioRxiv - Genetics 2022Quote: ... similar ten-fold serial dilutions were performed and equal volume from each dilution was plated on solid minimal medium (CO2) containing 1) either no MESNA (2-MercaptoEthane Sulfonate Na, Sigma, M1511-25G), or 2 ...
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bioRxiv - Microbiology 2022Quote: ... Each strain to be tested was then washed twice with sterile 1x PBS and adjusted to a concentration of ~2×105 cells/mL in macrophage growth media containing 1 μg/mL of the anti-GXM antibody 18B7 (Sigma Cat# MABF2069). Cells were opsonized in this media for 1h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... animals were first pre-incubated for 10 minutes in low Mg2+ HL3 (10 mM Mg2+, 0 mM Ca2+) containing 2 µM of phorbol ester Phorbol-12-myristat-13-acetat (PMA (Sigma Aldrich, Germany), stored at −20°C as 10 mM stock in DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... animals were pre-incubated for 10 minutes in HL3 (0 mM Ca2+) containing 2 µM of phorbol ester Phorbol-12-myristat-13-acetat (PMA (Sigma Aldrich, Germany), stored at −20°C as 10 mM stock in dimethyl sulfoxide (DMSO)) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were harvested by centrifugation at 4000 x g and 4°C for 30 min and the pellet was resuspended in 2 mL 50 mM Tris HCl (Carl Roth, #AE15.2) containing 25 mM NaCl (Sigma-Aldrich, #S3014-500G) at pH 8.5 ...
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bioRxiv - Plant Biology 2023Quote: ... nonfat dry milk diluted in KPBS [0.01 M Potassium Phosphate (pH7.1) containing 0.5 M sodium chloride and 2 mM Sodium Azide (Sigma-Aldrich, St. Louis, Missouri, USA) for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... cells were seeded onto 10-cm dishes with a ∼30% density and cultured for 18 h with pre-warmed fresh medium containing 2 mM thymidine (Sigma-Aldrich, 89270). The cells were next washed three times with pre-warmed 1xPBS and then incubated in pre-warmed fresh medium with no thymidine for 8 h ...
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bioRxiv - Immunology 2023Quote: ... The combined ethyl acetate extracts containing free fatty acids or their derivatives were concentrated to ∼500 μL under a gentle nitrogen stream and applied to a small silicic acid column (2 × 90 mm containing 30 mg of Type 60A, 100–200 mesh silicic acid, Sigma-Aldrich Korea). Extracts were sequentially eluted with 250 μL of increasingly polar solvents starting with 100% ethyl acetate ...
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bioRxiv - Physiology 2023Quote: ... glycerol:relaxing solution (in mM: 100 KCl, 10 imidazole, 2 EGTA, 5 MgCl2, and 4 ATP) containing 1X protease inhibitor cocktail (Sigma-Aldrich P8340) and 1% triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... in 30 ml Dulbecco’s minimal essential medium (DMEM) containing 1 % (v/v) penicillin/streptomycin and 2% FBS (F7524, Batch 024M3398, Sigma-Aldrich, Missouri, USA), at 37°C overnight in a rotary shaker ...
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bioRxiv - Microbiology 2023Quote: ... 10 pylorus and ileum sections from bees coming from the same cage were pooled in a 2 ml screw cap tube containing 750 μl of TRI reagent (Sigma-Aldrich, Merck), glass beads (0.75-1 mm diameter ...
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bioRxiv - Physiology 2023Quote: ... the atria and ventricles were dissected and separately minced in electrolyte solution containing 2% Bovine Serum Albumin (BSA; Sigma-Aldrich, Bornem, Belgium), dispersed by gentle agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then cultured in fresh RPMI medium containing 30 μM 5-chloro-2′-deoxyuridine (CIdU) (Sigma-Aldrich, St. Louis, MO) and incubated for an additional 4h ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated at a density of 500-1000 cells/cm2 in 6 well plates and allowed to grow for 2 weeks in normal culture media or growth media containing 10 ng/ml doxycycline (Sigma-Aldrich, D9891). Cultures were then fixed with 10% buffered formalin (Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... After blocking non-specific binding in 2% non-fat milk in 0.1 M PBS containing 0.1% Triton X-100 (PBS/MT; X-100, Sigma Aldrich, St. Louis, MO), the organs were incubated in goat anti-Shh primary antibody (Table 1 ...
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bioRxiv - Immunology 2023Quote: ... The lungs were harvested and grossly homogenized with scissors in HBSS containing 2 mg of collagenase D (Sigma Aldrich cat. no. 11088866001) and 0.25 mg of DNAse I (Sigma Aldrich cat ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde and the slide was mounted using a medium containing 4′,6-diamidino-2-phenylindole-DAPI (Fluoroshield, Sigma-Aldrich, USA). A fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera ...