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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Embryos and larvae were raised until 5 days post fertilization (dpf) in E3 medium containing methylene blue or in 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) if prevention of pigment formation was required ...
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bioRxiv - Neuroscience 2023Quote: ... The pelleted PRP was resuspended in PBS containing prostaglandin E1 (2 μM, Absin) and ethylene diamine tetra acetic acid (EDTA) (5 mM, Sigma-Aldrich) to prevent platelet activation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CHO cells were stably transfected with a SNAP-tagged human D2SR (short isoform) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 2 mM glutamine (Sigma-Aldrich, Poole, UK) and supplemented with 10% fetal calf serum (Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... Tissues were then transferred into an enzymatic digestion buffer (200 µL, in a 24-well plate) containing Dispase II (2 mg/mL, Sigma Aldrich) and Collagenase Type P (0.4 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... The kidneys were minced into <2 mm pieces and transferred to a Dounce tissue grinder containing 2 ml of lysis buffer (Nuclei EZ Lysis buffer, Sigma NUC101) supplemented with protease inhibitor (Sigma 5892970001 ...
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bioRxiv - Cell Biology 2023Quote: ... Minced samples were transferred into a digestion buffer (4 mL digestion buffer per 100 mg of muscle) containing 2 mg/ml Dispase II (D4693 Sigma-Aldrich) and 2 mg/ml Collagenase IV (17104019 Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... cells were centrifuged at 300 g for 5 minutes and resuspended in Essential 8 Flex medium containing 2 µM ROCK inhibitor Thiazovivin (Millipore Sigma). hPSCs were then counted using a Moxi Cell Counter (Orflo Technologies ...
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bioRxiv - Biophysics 2023Quote: ... bacteria were grown in isotope-enriched M9 minimal media containing 1 g/l of 15N-ammonium chloride and 2 g/l of 13C-glucose (Sigma, USA) supplemented with 1.7 g/l YNB (Yeast Nitrogen Base ...
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bioRxiv - Biophysics 2023Quote: ... Observation wells were then washed with 5x50 μl KG7 and 50 μl imaging buffer (KG7 containing 2 mM Trolox (Sigma-Aldrich), 1 mg/ml glucose oxidase (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μL of supernatant was incubated with 7.5 mM Tris buffer (pH 8.0) containing 50 μM DTNB (5,5′-dithiobis-(2-nitrobenzoic acid)) (Sigma‒Aldrich, MI, United States), 300 μM acetyl-CoA and 1 mM oxaloacetate ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 25 mg tissue/sample for a total of 8 samples per pool was combined and homogenized in a single cold douncer containing 2 ml ice-cold lysis buffer (Nuclei PURE Lysis Buffer (Sigma #L9286), 0.1M DTT from freshly thawed aliquot (Sigma #GE17-1318-01) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with PBG blocking buffer containing secondary antibodies and 0.4 μg/ml DAPI (4’,6-diaminido-2-phenylindole, Sigma, cat # D9542) for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... nuclei and actin were stained in PBST containing 5 µg/ml 4′,6-diamidino-2– phenylindole dihydrochloride (DAPI; D9542, Sigma-Aldrich) and Alexa Fluor 488 phalloidin (diluted in 1/100 ...
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bioRxiv - Microbiology 2023Quote: ... DKO cells were seeded into 25 cm2 flask in growth medium containing 2 µM 4OH-tamoxifen (Sigma, cat. no. H-6278) for 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... the medium was replaced with medium containing the corresponding treatments and 6h a pulse of BrdU (5-bromo-2-deoxyuridine, 50 µM; Sigma-Aldrich) was given to the neonatal cells ...
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bioRxiv - Immunology 2023Quote: ... Medium was removed 24 hours post-transduction and replaced with selective growth medium containing 2-ug/ml of puromycin or blasicidin (Sigma-Aldrich). Selective medium was changed every three days ...
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bioRxiv - Genomics 2022Quote: ... Each lobe was dissected off and minced into small pieces in a beaker containing 39 mL SC-2 supplemented with 1 mL DNase I (Sigma-Aldrich) and 20 mg collagenase P ...
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bioRxiv - Genomics 2023Quote: ... 0.05% digitonin) containing 2 mM EDTA and a 1:25 dilution of the 0.1 mg/mL BG4 primary antibody (Sigma-Aldrich: MABE917). Primary antibody incubation was performed overnight at 4°C and then the liquid was removed ...
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bioRxiv - Immunology 2023Quote: ... samples were serially diluted in MEM containing 2% FCS and 100 Units Penicillin / 0.1 mg Streptomycin (P/S) (Millipore Sigma, Germany). Vero E6 cells were incubated with 100 µl of ten-fold sample dilutions added in quadruplicates for 1 h at 37°C before 100 µl MEM containing 2% FCS and P/S were added per well and plates were incubated for 5 days at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 20 uL of 50 mM ammonium bicarbonate containing 2 µg of proteomics grade trypsin (Sigma Aldrich). Proteolytic digestion was performed overnight at 37 °C in a shaking incubator set to 200 rotations/minute.
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bioRxiv - Bioengineering 2023Quote: ... three samples of each concentration were incubated in 2 mL of a solution containing 1 mM toluidine blue (no. 89640; Sigma Aldrich) in PBS at pH 10 at room temperature on an orbital shaker set at 150 rpm (no ...
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bioRxiv - Microbiology 2023Quote: ... Absorbance was converted to the concentration of reduced iron(II) using a standard curve containing a 2-fold range of FeSO4 (Sigma-Aldrich) (0.25 mM to 0.016 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended and cultured in RPMI 1640 containing 15% FBS supplemented with 100 nM 2-mercaptoethanol and penicillin/streptomycin (Sigma; P4083).
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bioRxiv - Developmental Biology 2023Quote: ... 28 hpf manually dechorionated embryos were incubated at 28.5 °C in the dark into a 2 ml petri dish containing 0.4% DMSO in embryo water with LY-411575 (Sigma cat. SML0506) at a final concentration of 50 μM ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were mounted on glass microscopy slides with DAPI-containing mounting medium (4’,6-Diamidino-2-phenylindole dihydrochloride, Vectashield-H-1200-10, Sigma-Aldrich). All brain slices were imaged by a confocal microscope (LSM 710 ...
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bioRxiv - Pathology 2023Quote: ... and the whole brain of wildtype, heterozygous and homozygous mice (2 months, 6 months, 1 year) with phosphate buffer (PBS) containing 1% Triton-X100 (Sigma-Aldrich) and a Complete EDTA-free cocktail of protease inhibitors (Roche) ...
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bioRxiv - Immunology 2023Quote: ... 5 x 105 MDCK cells per ml were prepared in serum free DMEM containing 2% v/v penicillin/streptomycin (Sigma-Aldrich) and 2 µg/ml TPCK Trypsin (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... Each EC-patch was transferred by a wide bore pipette tip into 50 μL EGM-2 medium containing 4 U/mL thrombin (Sigma, T9549). Then the medium with a patch was mixed with 50 μL fibrinogen stock solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250). Lysates were separated on 4-12% BoltTM Bis-Tris gels (Invitrogen ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... the medium was replaced with 2 mL of prewarmed full growth medium containing 2 µg/mL of puromycin and 25 mM of sodium L-ascorbate (Sigma-Aldrich). Puromycin selection was performed for 2 days ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were randomized in two groups of which one received henceforth 2 mg/ml Dox (BelaDox, Bela-pharm) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce sh4E-BP1_2 expression ...
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bioRxiv - Neuroscience 2023Quote: ... Frozen brain tissue was homogenised in a dounce-homogeniser containing 2 ml ice-cold lysis solution (Nuclei EZ Lysis Buffer [Sigma-Aldrich, NUC101] or Nuclei PURE Lysis buffer [Sigma-Aldrich ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... Calcein-labeled transfected cells and control unlabeled cells were mixed at a 1:1 ratio and perfused onto a 25 mm-diameter glass coverslips (Marienfeld) previously coated with 20 μg/ml fibronectin and blocked with PBS containing 2% BSA (Sigma-Aldrich) in a microfluidic chamber on a microscope stage at 37°C ...
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bioRxiv - Physiology 2024Quote: Dissected intrascapular BAT and inguinal adipose tissues were incubated in high-glucose DMEM containing 2% fatty acid-free BSA (Sigma, A8806) for 30 minutes at 37C ...
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bioRxiv - Microbiology 2024Quote: ... BEAS-2B cells grown in 6-wells plates were first transduced with lentiviruses encoding Cas9 diluted in infection medium (DMEM, 2% FCS) containing 8 μg/ml Polybrene (Sigma Aldrich). At 16 h post transduction ...
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bioRxiv - Biochemistry 2023Quote: ... The enzymatic reaction was then initiated by adding 100 µL TE buffer containing 2 mM DTNB (Sigma-Aldrich, St. Louis, USA). The amount of liberated TNB was then quantified spectrophotometrically by measuring the absorbance at 412 nm for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in blocking buffer containing secondary antibodies and 0.5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich #D9542) for one hour ...
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bioRxiv - Molecular Biology 2024Quote: ... and plated at single-cell onto matrigel coated plates with induction media containing 10 μM Y-27632 and 2 μg/mL doxycycline (Sigma D9891) as previously described40 ...
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bioRxiv - Neuroscience 2024Quote: ... sciatic nerves or cocultures homogenates were prepared by tissue lyser disruption for 2 min in 250 µl of ice-cold methanol/water/acetonitrile 55:25:20 containing [U–13C6]–glucose (Sigma Aldrich) 1 ng/µl and [U–13C5]–glutamine (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... and mouse anti-ferret totIg (mAb 8H9 + mAb 4B10) diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich] ...
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bioRxiv - Immunology 2024Quote: ... sections were washed with PBS to remove OCT compound on the slides, and blocked with solutions containing imaging buffer (2% FCS, 0.1% Triton X-100 (Sigma, Cat. X100), 0.01% sodium azide) ...
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bioRxiv - Microbiology 2024Quote: ... Donor and recipient cells were mixed to a ratio of 10:1 and 2 µL of the mating was spot into a microscope slide containing an M9 (M9 salts (Sigma-Aldrich), 500M mM MgSO4 ...
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bioRxiv - Microbiology 2024Quote: ... Standard protocol was used for DNA extraction with the CTAB lysis buffer containing 2% w/v CTAB (Sigma-Aldrich, Poole, UK), 100 mM Tris–HCl (pH = 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Physiology 2022Quote: ... Total protein concentration in the samples was determined with a standard BSA kit (Millipore). 15 µL of the sample was diluted with 60 µL ultrapure water in order to keep the concentration within the linear range of the calibration curve (0.2 to 2 µg⋅µL−1) ...
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bioRxiv - Systems Biology 2020Quote: ... Total protein concentration was measured using bicinchoninic acid assay (BCA Kit, Sigma-Aldrich, USA).
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bioRxiv - Cell Biology 2021Quote: ... was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich). PDMS stamps were incubated with solution containing 50μg/ml fibronectin and 1μg/ml of the conjugated fibronectin in Dulbecco’s phosphate-buffered saline (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: RIP assays employed the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: RIP assay was done with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA). Quantitative RT-PCR was used for determining total RNA levels.
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated with the Quantipro BCA assay kit (Sigma-Alrich, United States), and lysates were resolved on a 10% sodium dodecyl sulfate polyacrylamide gel prior to transfer onto polyvinylidene fluoride membranes ...