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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... eggs were fixed in 2% paraformaldehyde in PBS for 20 min and permeabilized in PBS containing 0.2% Triton X-100 (Sigma-Aldrich, 900-93-1) for 20 min ...
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bioRxiv - Immunology 2020Quote: ... PP and MLN were lysed in 2-mL tubes containing one steal bead and 1 mL PBS + 0.1% Tween20 (Sigma-Aldrich, St. Louis, USA), using a QiaTissueLyzer (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... 0.2 ml saline (0.9%) solution containing 1 mg Al (OH)3 as an adjuvant and 20 µg BLG (Sigma, St. Louis, MO, USA). Control mice received 0.2 ml saline (0.9% p/v ...
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bioRxiv - Neuroscience 2020Quote: ... MNs were first washed with 2 mL of dPBS (calcium and magnesium free phosphate buffered saline) containing 0.04% BSA (Sigma-Aldrich; Cat. No. A7906) and dissociated with 2 mL of dissociation reagent containing papain (50U ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed again with Perm/Wash Buffer and FACS buffer and fixed in PBS containing 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO). For each sample ...
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bioRxiv - Biochemistry 2022Quote: ... a RRP17-HA-AID strain was transformed with plasmids carrying respective constructs and growth assessed upon Rrp17-HA-AID depletion on plates containing 2 mM auxin (3-indoleacetic acid) (Sigma-Aldrich, Cat# I2886). Genetic experiments were performed at least in two biological replicates.
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bioRxiv - Microbiology 2022Quote: ... The precipitates were extracted into 1x SDS sample buffer containing 2-mercaptethanol and subjected to a standard immunoblotting assay using HRP-conjugated anti-HA antibody (H6533; Sigma; 1:3000 dilution) and anti-FLAG antibody (A8592 ...
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bioRxiv - Neuroscience 2023Quote: ... in a staining jar and incubated for 2 h at room temperature in blocking solution containing 5% normal goat serum (Millipore Sigma, Item# G6767) in 1% Triton X-100 (Millipore Sigma ...
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bioRxiv - Immunology 2022Quote: ... for 10 min at room temperature before washing with PBS containing 2% FBS and 0.1% sodium azide (FACS buffer; Sigma Aldrich, St. Louis, MO). Cells were then stained with the following antibodies ...
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bioRxiv - Physiology 2023Quote: ... bundles were rinsed twice in Rigor solution containing 2 mM DTT and 1:200 dilution of protease inhibitor (Sigma-Aldrich, St. Louis, MO) before being homogenized for 1 or 2 pulses of 30s at high speed ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was started by addition of 25 μL ZDHHC master mix containing 20 nM ZDHHC20 and 2 μL of α-ketoglutarate dehydrogenase (KDH, Sigma-Aldrich, K1502). In the first reaction step ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed again with Perm/TF Wash and FACS buffer and fixed in PBS containing 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO). A total of 100,000 events were acquired by the LSRII (Becton Dickinson ...
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bioRxiv - Immunology 2023Quote: ... Media was removed from the apical compartment of the transwell and replaced by 200µL of fresh medium containing of 2 mg/mL fluorescein isothiocyanate (FITC)-labelled dextran (4 kDa) (Sigma-Aldrich, # 46944-500MG-F). As a negative control and to calculate the background ...
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bioRxiv - Microbiology 2023Quote: ... and rAgApyrase in the wells of a 96-well microtiter plate with a final volume of 0.1 ml containing 2 mM of either ATP (Millipore-Sigma, Burlington, MA, USA, #A26209) or ADP (Millipore-Sigma ...
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bioRxiv - Systems Biology 2023Quote: ... and lower chambers (ventricles) were dissected after digestion and separately minced in electrolyte solution containing 2% Bovine Serum Albumin (BSA; Sigma-Aldrich, Bornem, Belgium), dispersed by gentle agitation ...
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bioRxiv - Immunology 2023Quote: ... 6X SDS-PAGE sample loading buffer (G Biosciences, St. Louis, MO #785-701) containing 5% v/v 2-mercaptoethanol (Sigma-Aldrich #M3148-100ML) was added to lysates ...
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bioRxiv - Neuroscience 2023Quote: ... according to manufacturer’s instructions in dopaminergic neurons after 30 days of differentiation using 600 µl RLT lysis buffer containing 1:1,000 2-mercaptoethanol (Sigma Aldrich, cat. no M3148). After RNA extraction ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µM ecTrxB or bsTrxB were added to TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5) containing 200 µM NADPH (Sigma-Aldrich, St. Louis, USA) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed with PBS (3x10 min at room temperature) and incubated for 2 hours at room temperature in blocking solution containing 5% normal goat serum (Millipore Sigma, Item # G6767) in 1% Triton X-100 (Millipore Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... at least 3 times and incubated at room temperature for 2 h in blocking solution containing 5% normal horse serum (Sigma-Aldrich, Cat. # H0146) in 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Low passage HeLa cells (ATCC, CCL-2) were transduced with the lentivirus in media containing 8ug/mL polybrene (Sigma Aldrich, TR-1003-G) for 48 hours ...
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bioRxiv - Biochemistry 2024Quote: ... in 100 µL TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5) containing 200 µM NADPH (Sigma-Aldrich, St. Louis, USA). Protein concentration was determined through absorption at 280 nm using the molar extinction coefficients 18910 M-1cm-1 (ecTrxB) ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells (2×105 cells/mL) were incubated in the culture medium containing 200 ng/mL of phorbol myristate acetate (PMA) (Sigma-Aldrich; Merck KGaA) for 24 h to induce differentiation into macrophage cells ...
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bioRxiv - Neuroscience 2021Quote: ... corresponding to the external Ig-like domain) diluted in sterile PBS and emulsified in an equal volume of incomplete Freund’s adjuvant (IFA, Sigma; total volume 100µl per rat). IFA control rats were immunised with PBS emulsified in an equal volume of IFA ...
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bioRxiv - Molecular Biology 2023Quote: A pET28-mhl vector containing the coding region for the SETDB1 Triple Tudor domain (residues 196-403 of NP_036564) was transformed into Rosetta2 BL21(DE3)pLysS competent cells (Novagen, EMD Chemicals, San Diego, CA). A 6 L culture was grown to mid log phase at 37 °C at which time the temperature was lowered to 18 °C and protein expression was induced by addition of 0.5 mM IPTG ...
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Microbiology 2019Quote: ... in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies: mouse anti-RSV fusion protein monoclonal antibody (MAB8599, Millipore, USA) or goat anti-human IgG (Licor ...
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bioRxiv - Cell Biology 2021Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165; used at 1:2500) in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... and stained with sACE2-humanFc fusion protein (construct is a gift of Jason McLellan, University of Texas at Austin) or mouse anti-Flag antibody (Sigma, F3165). Cells were then stained with anti-human-IgG-FITC (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 80 µg protein were loaded into a 10 % SDS gel and stained with anti-Tau (clone Tau46, T9450, mouse, 1:1000, Sigma-Aldrich).
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was then added to the Protein G sepharose beads coupled to either HA 12CA5 mouse monoclonal IgG (Sigma Aldrich) or anti-Ty1 (BB2 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight at 4 °C with mouse anti-SMI31/32 (to label neurofilament proteins; Millipore NE1022/NE1023; 1:1000) and chicken anti-myelin basic protein (to label myelin ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
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bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
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bioRxiv - Cell Biology 2020Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ubiquitin proteins antibody (clone FK2; Enzo, New York, USA, Cat#BML-PW8810 and Sigma, Gothenburg, Sweden, Cat#04-263), guinea pig polyclonal anti-VGLUT1 (1:5000 ...
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bioRxiv - Developmental Biology 2022Quote: ... FLAG-tagged constructs were then immunoprecipitated with Dynabeads Protein G beads coupled to mouse anti-FLAG M2 mAb (Sigma #F3165, RRID:AB_259529) for 3 hours at 4°C with rotation ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2022Quote: ... the following primary antibodies were incubated overnight at 4 °C in a humidified chamber: mouse anti-glial fibrillary acidic protein (GFAP) (1:500; Sigma-Aldrich), rabbit anti- laminin 1α (1:200 ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp. ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Genomics 2023Quote: ... The following primary antibodies are used for the first target protein at the dilutions indicated: NeuN (1:500, A60, Mouse, Millipore MAB5374), pTDP43 (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then incubated overnight with primary antibodies against Yes-associated protein (YAP) (mouse monoclonal anti-YAP, 1:400, Sigma-Aldrich) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody ...