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Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... or alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for Elisa (Sigma P7998) were added as a substrate ...
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bioRxiv - Immunology 2022Quote: ELISA plates were coated overnight with anti-human IgG (Sigma Aldrich I5260) at 1:5 000 in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... containing protease (Sigma) and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... containing rLysozyme (Millipore) and Benzonase nuclease (Millipore ...
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bioRxiv - Immunology 2022Quote: ... containing PBS (Sigma) with 2% fetal bovine serum (FCS ...
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bioRxiv - Neuroscience 2019Quote: ... containing blebbistatin (Sigma), followed by centrifugation at 300Xg for 5min to pellet the cells ...
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bioRxiv - Immunology 2023Quote: ... containing PBS (Sigma) with 2% fetal bovine serum (FCS ...
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bioRxiv - Developmental Biology 2023Quote: ... containing PMSF1mM (Sigma), and 1X Protease Inhibitor (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore, cat#UFC805024). The concentrated sample was stored in 50% glycerol at −20°C.
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged at >10,000 rpm to remove cellular debris and the supernatant containing extracted protein was transferred to a black flat-bottom microwell plate (Millipore Sigma, M9685). Relative fluorescence intensity of each sample was measured on a SpectraMax iD3 microplate reader (Molecular Devices ...
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bioRxiv - Biochemistry 2020Quote: ... The column was washed with 10 column volumes of wash buffer and proteins were eluted with 8 ml of the same buffer containing 10 mM maltose (Sigma-Aldrich M5895). The eluate was applied to a HiLoad 16/600 Superdex200 pg column (GE Healthcare 28-9893-35 ...
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bioRxiv - Immunology 2020Quote: Frozen tissues were homogenized in 10 volumes (mg : µl) of T-PER tissue protein extraction reagent (Thermo, Cat#78510) containing cocktail inhibitor (Sigma, Cat#P8340) and PMSF ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Microbiology 2020Quote: ... The affinity gel was then pelleted and washed twice using TEN buffer with 0.1% NP-40 and resuspended in equal volumes of NuPage LDS Sample Buffer (Thermo) containing 143 mM 2-Mercaptoethanol (Sigma-Aldrich). Western blotting using the elutes from affinity purification and the pre-purified input lysates were performed as described above with the following primary antibodies ...
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bioRxiv - Biophysics 2021Quote: HeLa H2B-mCherry day 2 aggregates were equilibrated for 1 h in CO2 independent medium containing 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich) and 1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were randomized in two groups of which one received henceforth 2 mg/ml DOX (BelaDox, Bela-pharm) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce TCF7L1 re-expression (DOX (+)) ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were randomized in two groups of which one was henceforth treated with 2 mg/ml DOX (Beladox, Bela-pharm, Germany) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce an in vivo re-expression (DOX (+)) ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant was either directly added to an equal volume of 2X Laemmli buffer containing 2% β-mercaptoethanol or precipitated with trichloroacetic acid (Sigma) and washed with acetone prior to resuspension in 2X Laemmli containing 2% of β-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... or human NUP153-specific shRNA lentivirus particles overnight at 37°C followed by selection in medium containing Puromycin (Puro, 2 μg/ml) (Sigma-Aldrich) for 48 hr ...
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bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
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bioRxiv - Developmental Biology 2021Quote: ... 0 dpf embryos were transferred to 10 cm petri dishes containing 40 ml of labeling media either with or without 2 mM H2O2 (Sigma-Aldrich) at a density of 1000ul of embryo medium/embryo and kept at 28°C ...
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bioRxiv - Developmental Biology 2021Quote: ... fixed cells were permeabilized with PBS containing 0.2% Triton X-100 for 10 min and blocked with PBS containing 1% IgG-free bovine serum albumin (BSA) (Sigma, A2058) for 30 min at RT ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and the suspension was transferred into a 2 mL tube containing 50 mg of 425 – 600 μm acid-washed glass beads (Sigma-Aldrich). The bacterial cells were disrupted using a Fast prep FP120 homogenizer (Thermo Savant Bio101 ...
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bioRxiv - Cell Biology 2022Quote: ... lentivirus at 1×106 pfu/ml per plasmid (multiplicity of infection (MOI) of 2) were added in fresh mTeSR medium containing 4μg/μl polybrene (Sigma, TR-1003). On the day 0 ...
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bioRxiv - Cell Biology 2019Quote: ... muscles were incubated for 60min at 37 °C in the presence of external Tyrode containing 2 mg.mL collagenase (Sigma, type 1). Single fibers were obtained by triturating the collagenase-treated muscles within the experimental chamber.
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bioRxiv - Cell Biology 2020Quote: ... washed with 1x PBS and fixed on slide with methanol followed by DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich) to stain nuclear and kinetoplast DNA and analysed immediately ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed in methanol and then stained with DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich). Cells were visualized using a Olympus IX 81 microscope with FV1000 confocal system using a 100X oil immersion Plan Apo (N.A ...
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bioRxiv - Neuroscience 2019Quote: ... and P30 frontal cortex tissue (collected in Experiment 2) using RIPA buffer containing cOmplete Mini Protease Inhibitor Cocktail tablets (Sigma-Aldrich). Protein concentrations were quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... cells at 14 DIV were treated with culture medium containing 100 µg/ml L-ascorbic acid 2-phosphate (vitamin C, Sigma, 49752) for 24 h ...
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bioRxiv - Cell Biology 2019Quote: ... Medium was removed and cells were incubated for another hour (total infection time: 2 h) at 37 °C in FBS-containing culture medium supplemented with 20 μg/mL gentamicin sulfate (Sigma-Aldrich), to kill extracellular bacteria ...
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bioRxiv - Molecular Biology 2020Quote: ... and transferred into pre-chilled 2 ml Eppendorf tubes containing 450 µl of pre-chilled RNAse-free 425-600 μm diameter glass beads (Sigma G8772). Cells were lysed by vortexing at maximum speed for 5 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Medroxy Progesterone Acetate and cAMP) medium (1x Opti-MEM reduced-serum media containing 2% FBS, 100 nM estradiol [cat. no. E1024, Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were incubated at 37 °C for 30 min to achieve maximum polymerization and then were mixed in a 1:1 volume ratio with pre-warmed PM buffer containing 3 mM MgCl2 and 2% Methylcellulose (MO512, Sigma-Aldrich). Final concentrations of protein ...
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bioRxiv - Bioengineering 2020Quote: ... Serial dilutions of the samples (1:2 to 1:64) and a standard row containing 1 to 40 nmol Neu5Ac (Sigma Aldrich) were prepared in extraction buffer ...
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bioRxiv - Bioengineering 2020Quote: ... The cell culture medium was replaced with 2 mL fresh medium containing 8 µg/mL hexadimethrine bromide (Sigma Aldrich, H9268-5G), 20 µL ViralPlus Transduction Enhancer (Applied Biological Materials Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... glycans were co-crystallized with a solution of 50% acetonitrile/50% 20 mM ammonium citrate containing 134 mM of 2’,4’,6’-Trihydroxyacetophenone monohydrate (Sigma-Aldrich). MALDI-ToF MS analysis of the N-glycans was performed in the reflector positive and negative ion mode using a 4800 Plus MALDI ToF/ToF Analyzer (AB Sciex ...
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bioRxiv - Cancer Biology 2021Quote: ... after that glutamine-free medium in rescue condition was replaced by the medium containing 10% of dialyzed FBS and 2 mM of dimethyl-alpha-ketoglutarate (α-KG) (Sigma). Cells were incubated for additional 24 h and used for clonogenic survival assay.
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bioRxiv - Cancer Biology 2021Quote: ... For induction of the RRM2 knockdown 2 mg/ml Dox (BelaDox, Bela-pharm, Germany) was added in drinking water containing 5% sucrose (Sigma-Aldrich) for ad libitum uptake (Dox (+)) ...
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bioRxiv - Biochemistry 2020Quote: ... and were subsequently resuspended in 200 µL buffer A and transferred to 2 mL screw cap tubes containing 0.5 mL glass beads (Sigma Life Science). The cells were lysed using a Mini Bead Beater (BioSpec Products ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating tissue sections were washed three times for 10 minutes with PBS then incubated for 2 h in a PBS-blocking solution containing 0.5 % Saponin (Sigma Aldrich 84510) and 10 % pre-immune goat serum (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Axonal ChR2 expression was revealed by incubating slices for 1-2 h in Triton PBS containing 10% v/v NDS (D9663, Sigma, RRID:AB_2810235), then overnight at room temperature in Triton PBS containing 1% v/v NDS and Alexa Fluor 488-conjugated rabbit anti-GFP (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... for 45 minutes followed by 2 washes in a PBS buffer containing 1% FBS and 0.5% Saponin (Sigma, St Louis, MO). Intracellular staining for anti-IFNγ (2µg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 3X with EGM-2 medium and re-suspended in PBS containing 3 mg/ml of fibrinogen (Sigma, #F8630) and 0.15 U/ml of aprotinin (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell culture medium was replaced with 2 mL fresh medium containing 8 µg/mL hexadimethrine bromide (Sigma Aldrich, H9268-5G), 20 µL ViralPlus Transduction Enhancer (Applied Biological Materials Inc. ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... All embryos of 26 hpf or older were raised in fish water containing 200 μM 1-Phenyl-2-thiourea (PTU; Sigma Aldrich) to prevent melanin synthesis ...
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bioRxiv - Bioengineering 2022Quote: ... The two-post array supporting the newly formed hydrogels was then lifted out of the casting plate and transferred to a fresh 24-well plate containing Lonza growth medium supplemented with 2 g/L aminocaproic acid (Sigma-Aldrich) to inhibit fibrinolysis ...
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bioRxiv - Cancer Biology 2022Quote: ... 2×106 MOLM-14 or MV4-11 cells were resuspended in a medium containing 8μg/mL polybrene (Sigma-Aldrich, Cat# 107689) and 2 mL of thawed lentivirus-containing HEK 293T/17 supernatant ...
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bioRxiv - Biophysics 2022Quote: ... cells were transfected with 100 μL of 1X PBS containing 2 μL of X-tremeGENE HP DNA transfection reagent (Sigma,6366244001) and 2 μL plasmid DNA (300-400 ng/μL ...
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bioRxiv - Genetics 2022Quote: ... The pellet was resuspended in 1x PBS and cells were stained with a solution containing 1 µg/ml 4,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich India) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue pieces containing Po were stained with 2% uranyl acetate (Agar Scientific, AGR1260A) in 0.1M sodium acetate buffer (Sigma-Aldrich, S2889) for 45 min–1 h and dehydrated through a graded series of methyl alcohol (70% ...