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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The mouse Th17 9-plex Milliplex Map Kit (EMD Millipore, Catalog # MTH17MAG-47k) was used to quantify murine TNFα ...
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bioRxiv - Microbiology 2020Quote: ... The mouse serum was collected for the Arginase Activity Assay kit (Sigma, MAK112).
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bioRxiv - Cell Biology 2022Quote: ... using the Duolink In Situ Red Starter Kit Mouse/Rabbit (#DUO92101, Sigma-Aldrich), according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Cell Biology 2023Quote: ... The Duolink® In Situ Orange Starter Kit mouse/rabbit (Sigma Aldrich, USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The genotyping process was conducted using the KAPA mouse genotyping kit (Millipore Sigma) using the following primer pairs ...
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bioRxiv - Microbiology 2020Quote: ... The assay was performed using a commercial kit (Duolink In Situ Red Starter kit Mouse/Rabbit, #DUO92101, Sigma-Aldrich) following the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... Five 15 cm dishes of 70 – 80 % confluent mouse Schwann cells were separately transfected with 30 μg of pLKO.1-puro vector containing five commercial VDAC1 shRNA (Sigma-Aldrich, sh1#TRCN0000012388 ...
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bioRxiv - Microbiology 2020Quote: ... mouse polyclonal antibody was produced immunizing a mouse with 50 mg of purified recombinant MtEPSPS containing Freund’s incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was removed entirely and cells were suspended in 100 μl of the same TBS solution containing mouse monoclonal ANTI-FLAG M1 antibodies (Sigma, F3040) at a 1:500 dilution ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663 ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were then incubated overnight at 4°C in PBST-NGS containing mouse monoclonal anti-α-Tubulin-FITC antibody (Sigma-Aldrich) at a dilution of 1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 and 10% foetal calf serum in PBS] containing primary antibodies including: mouse anti-NeuN (1:200; Millipore, #MAB337); mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... for 30 minutes at room temperature and then overnight at 4°C in blocking solution containing mouse anti-Alpha-tubulin (1:500, Sigma, #T9026). Next ...
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bioRxiv - Neuroscience 2023Quote: ... sections were re-blocked in blocking buffer for 1 hour at room temperature then incubated in primary antibody solution containing mouse anti-5-HT1A receptor (Millipore Sigma, cat ...
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bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...
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bioRxiv - Physiology 2024Quote: Whole lysates were isolated from cells (C2C12 and HepG2) and mouse tissues (heart, liver, and skeletal muscles) with RIPA lysis buffer containing protease inhibitor (Sigma, P8340) and phosphatase inhibitors (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA extraction was performed by lysing a fragment of mouse tail in a lysis buffer containing EDTA (#E9884, Sigma Aldrich), SDS (CBMSO facility ...
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bioRxiv - Immunology 2024Quote: ... The wells were rinsed three times with PBS containing 0.05 % Tween 20 and horseradish-peroxidase-labeled rabbit anti-human or anti mouse IgG (100 μL/well; Sigma-Aldrich, Germany) was added to each well and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: Cytokines from mouse brain homogenates prepared in section 2.7 were measured using Millipore mouse cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA). Calibration curves from recombinant cytokine standards were prepared with threefold dilution steps in the same matrix as the samples ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Cells were then incubated for 15 minutes in 2% BSA with globular actin-binding protein GC globulin (Sigma). Following incubation ...
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bioRxiv - Biochemistry 2021Quote: 100 bacterial homologs of nucleobase/cation symporter 2 (NCS2) proteins were cloned into a modified pET28 plasmid (Novagen) with either an N- or a C-terminal deca-histidine tag and a TEV protease recognition site at the central facility of the New York Consortium on Membrane Protein Structure (NYCOMPS ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was treated by 0.5 mg/mL drICE protease and incubated with 2 mM NADPH (Sigma-Aldrich) for 2 h at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Genetics 2019Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich, St. Louis, MO, product C4706) and alkylated with 55 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Protein complexes were released from chromatin by digestion with 2 U/μl of Benzonase nuclease (E1014-25KU, Sigma) and sonicated for 5 min using a Diagenode sonicator with settings ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 hours and subsequently incubated with Dynabeads Protein G and an anti-Flag antibody (Sigma-Aldrich, F1804) at 4°C with rotation for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10, Millipore). After washing with dilution buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Expression of the eGFP protein and the PAK1 mutant was induced by doxycycline (2 μg/mL, Sigma-Aldrich, St ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then incubated for 15 minutes in 2% BSA with globular actin-binding protein GC globulin (Sigma). Following incubation ...
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bioRxiv - Microbiology 2023Quote: ... Enriched proteins were denatured in U/T buffer (6 M urea, 2 M thiourea, 1 mM DTT (Sigma), 10 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... The nuclear protein lysate then was pre-cleared by adding 2 μL of poly D[IC] (Sigma-Aldrich) 25 µg/µL and 20 μL of High Capacity Strepavidin agarose beads (Thermofisher #20357) ...
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bioRxiv - Biophysics 2023Quote: ... The purified protein (in 25 mM piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES, P-1851, Sigma-Aldrich) pH 6.8 ...
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bioRxiv - Developmental Biology 2023Quote: ... The proteins bound to the beads were reduced with 10 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma, C4706) at 40°C for 30 min and alkylated with 20 mM Iodoacetamide (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
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bioRxiv - Cell Biology 2023Quote: ... The separation of the hydrophobic and hydrophilic proteins from the pellet was carried out with CelLyticTM MEM Protein Extraction Kit (Sigma-Aldrich, MO, USA) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... Their relative expression was monitored by ELISA using anti-FLAG-HRP (Sigma) or anti-HA-HRP antibodies (Roche Diagnostics) ...
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bioRxiv - Biochemistry 2021Quote: ... or alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for Elisa (Sigma P7998) were added as a substrate ...
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bioRxiv - Immunology 2022Quote: ELISA plates were coated overnight with anti-human IgG (Sigma Aldrich I5260) at 1:5 000 in PBS ...
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bioRxiv - Physiology 2024Quote: ... Serum insulin was measured by commercial ELISA (EMD Millipore cat. EZRMI-13K).
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bioRxiv - Cell Biology 2020Quote: ... containing protease (Sigma) and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... containing rLysozyme (Millipore) and Benzonase nuclease (Millipore ...
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bioRxiv - Immunology 2022Quote: ... containing PBS (Sigma) with 2% fetal bovine serum (FCS ...
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bioRxiv - Neuroscience 2019Quote: ... containing blebbistatin (Sigma), followed by centrifugation at 300Xg for 5min to pellet the cells ...
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bioRxiv - Developmental Biology 2023Quote: ... containing PMSF1mM (Sigma), and 1X Protease Inhibitor (Roche) ...
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bioRxiv - Immunology 2023Quote: ... containing PBS (Sigma) with 2% fetal bovine serum (FCS ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore, cat#UFC805024). The concentrated sample was stored in 50% glycerol at −20°C.
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged at >10,000 rpm to remove cellular debris and the supernatant containing extracted protein was transferred to a black flat-bottom microwell plate (Millipore Sigma, M9685). Relative fluorescence intensity of each sample was measured on a SpectraMax iD3 microplate reader (Molecular Devices ...
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bioRxiv - Biochemistry 2020Quote: ... The column was washed with 10 column volumes of wash buffer and proteins were eluted with 8 ml of the same buffer containing 10 mM maltose (Sigma-Aldrich M5895). The eluate was applied to a HiLoad 16/600 Superdex200 pg column (GE Healthcare 28-9893-35 ...