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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... IL-22) were determined using the Mouse Th17 Magnetic Bead Panel Milliplex MAP kit (Millipore). Samples were not diluted and assay was performed according to the manufacturer’ instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... using the Mouse Metabolic Magnetic bead panel kit (Reference: MMHMAG-44K – Milliplex map by Millipore). Corticosterone was measured by RIA using Corticosterone 125I RIA kit for rats & mice (MP biomedical ...
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bioRxiv - Cancer Biology 2020Quote: ... rabbit and mouse probes from the Duolink In Situ Orange kit (Cat.#DUO92102; Sigma-Aldrich) or the Duolink flowPLA Orange kit (Cat.#DUO94003 ...
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bioRxiv - Cell Biology 2021Quote: Proximity ligation assay was performed using a Duolink In Situ Orange Mouse/Rabbit Kit (Sigma) according to the manufacturer’s instructions with the following modifications ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... DuolinkTM In Situ PLA Mouse/Rabbit kit with red detection reagents (Sigma-Aldrich, DUO92101-1KT) was used according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: PLA was performed with the DuoLinkTM In Situ Red Starter Kit Mouse/Rabbit (Millipore-Sigma) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: PLA was performed with the DuoLinkTM In Situ Red Starter Kit Mouse/Rabbit (Millipore-Sigma) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... The PLA assays were performed using Duolink In Situ green kit Mouse/ Rabbit (Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and Duolink In Situ Red Starter Kit (Mouse/Rabbit; cat. DUO92101) were purchased from Sigma. Penicillin-streptomycin (for DMEM preparation ...
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bioRxiv - Cell Biology 2023Quote: ... DuolinkTM In Situ PLA Mouse/Rabbit kit with red detection reagents (Sigma-Aldrich, DUO92101-1KT) was used according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... the DuolinkTM In Situ Green Starter Kit Mouse/Rabbit (Sigma-Aldrich, St. Louis, MO, USA) was used ...
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bioRxiv - Physiology 2023Quote: Glycerol was measured in mouse plasma samples using the Glycerol Assay Kit (MAK117, Sigma-Aldrich) following the procedure recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: PLA was performed using the DuoLink In Situ Red Starter Kit Mouse/Rabbit (DUO92101, Sigma). µ-slide 8-wells (Ibidi ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed on 2 mg of total protein by incubating 2h at 4°C with anti-Flag agarose beads (Sigma). Beads were then washed in lysis buffer and bound polypeptides analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Immunology 2022Quote: ... stimulated with SARS-CoV-2 spike protein overlapping peptide library or stimulated with 5μg/ml of Phytohaemagglutinin (PHA) (Sigma-Aldrich) as a positive control ...
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bioRxiv - Biochemistry 2021Quote: ... 1mL (2 mg) of protein was added to 20 µL of ANTI-FLAG® M1 Agarose affinity Gel (Sigma Aldrich) pre-equilibrated in TBS and incubated on a nutator for 2 hours at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... The protein was expressed and purified the same way as SARS-CoV Mpro-2 but Factor Xa protease (Sigma, Canada) was used (4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Biochemistry 2021Quote: ... Insoluble material was removed by centrifugation at 149,000 ×g for 45 min and the solubilized protein was loaded onto a gravity column of 2 mL M2 anti-FLAG affinity matrix (Sigma). The column was washed with 10 mL of wash buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Residual methylation reagents were neutralised by addition of 1M Tris-HCl pH 7.5 to a final concentration of 50 mM Tris-HCl and the protein complex was concentrated to 2 mg mL−1 using 30 kDa cut-off concentrators (Millipore) before injection into a S75 16/600 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Plant Biology 2021Quote: ... the total protein was extracted from tobacco expressing CmTPL1-2-HA and CmSVP-FLAG using anti-HA affinity gel (E6779, Sigma), followed by western blotting with anti-FlAG (A8592 ...
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bioRxiv - Plant Biology 2021Quote: ... Wild-type and mutated Pik-1 and Pik-2 proteins were detected probing the membrane with anti-FLAG M2 antibody (Sigma) and anti-HA high affinity antibody 3F10 (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... The purified protein was then concentrated to 300 μM using an Amicon-2 Ultra concentrator (Millipore Sigma, product number UFC2030). DTT was added to the concentrated protein to a final concentration of 1 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... then 1-2 mg (as indicated) of total protein was incubated with pre-washed M2 α-FLAG magnetic beads (Sigma) or MagStrep “type3” XT beads (IBA ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Cell Biology 2019Quote: ... from bacteria were performed as previously described (Llauró et al., 2018) except that proteins were expressed in Rosetta 2(DE3)pLys cells (Novagen).
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bioRxiv - Immunology 2020Quote: ... 0.5 mL of SARS-CoV-2 S-2P protein was mixed with either an equal volume of Sigma Adjuvant System S6322 (Sigma), 600 μg/mL Adju-Phos aluminum phosphate (Brenntag) ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Cell Biology 2023Quote: For LC-MS/MS, proteins were reduced and alkylated in SDC buffer (1% Sodium deoxycholate, 40 mM 2-Cloroacetamide (Sigma), 10 mM TCEP (Thermo ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Neuroscience 2024Quote: ... The protein sample after elution was collected and concentrated to 2 mg/mL using a 50kDa molecular weight cutoff concentrator (Millipore).
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-mouse αSMA (Sigma A2547), goat-anti-human TIE1 (R&D AF619) ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The fractions containing GRP78 protein were combined and concentrated by centrifuging through 30-kDa Amicon ultra-15 centrifugal filter units (EMD Millipore, Billerica, MA, USA). A solution of GRP78 protein was prepared at the concentration of 6 mg·ml-1 in 50 mM NaHCO3 buffer for the binding experiments.
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bioRxiv - Immunology 2022Quote: ... and then lysed for 15 min with a M-PER Mammalian protein extraction reagent (Pierce Biotechnology, Rockford, IL) containing a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and 1 mM p-amidinophenyl methanesulfonylfluoride (PMSF) ...
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bioRxiv - Neuroscience 2023Quote: ... cells from mixed glial cultures were collected and protein was digested using a lysis buffer containing Triton X-100 (Sigma Aldrich, St. Louis, MO) and supplemented with protease and phosphatase inhibitors (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2023Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore, Car-rigtwohill Co. Cork TRL). Concentrated proteins were aliquoted and stored at -80°C in sizing buffer.
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bioRxiv - Cell Biology 2023Quote: ... and protein was extracted in a modified RIPA buffer containing protease and phosphatase inhibitors (P8340 and 524624, respectively; Sigma-Aldrich, St. Louis, MO, USA). Proteins were detected by immunoblotting using standard techniques ...
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bioRxiv - Molecular Biology 2023Quote: AKT:FBP1:ALDOB:PP2A-C interactions in liver tissue were detected with an in situ PLA kit (Duolink® In Situ Red Starter Kit Mouse/Rabbit, Sigma, DUO92101) (Su et al. ...
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bioRxiv - Physiology 2023Quote: ... FGF21 was measured by using a commercially available enzyme-linked immunosorbent assay kit (rat/mouse FGF21 enzyme-linked immunosorbent assay kit; Millipore, Billerica, MA), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... NY) diluted 1:10,000 in a PB solution containing 2% NHS and 0.3% Triton X-100 (Sigma Chemical Co., St. Louis, MO, USA). After washing in PB ...
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bioRxiv - Biophysics 2022Quote: ... the pancreas was transferred to a foil-covered glass vial containing a 1:1 solution of MeOH (100%) and BABB (1:2 (v/v) benzyl alcohol (402834-500ml, Sigma-Aldrich, Gillingham, UK) to benzyl benzoate (B6630-1L ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were performed in 6 μL volumes on 384-well plates in reaction buffer containing 3 μL of 2 x Kapa Probe Fast qPCR Master Mix (Sigma-Aldrich, Dorset, UK). All probes were supplied by Thermo Fisher Scientific (Massachusetts ...
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bioRxiv - Cell Biology 2022Quote: ... All iBAT pieces from one mouse were transferred into a well of a 12-well dish containing 0.5 ml high-glucose DMEM supplemented with 2% FA-free BSA (A6003, Sigma-Aldrich, St. Louis, MO) and 0.4 µCi [14C(U)]-D-glucose (ARC0122 ...
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bioRxiv - Cancer Biology 2019Quote: ... and finally with the refractive index matching solution BABB-D4 containing 4 parts BABB (benzyl alcohol + benzyl benzoate 1:2, Sigma, 24122 and W213802), 1 part diphenyl ether (DPE ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates from different groups were collected and incubated with magnetic bead RIP buffer containing anti-human argonaute 2 (Ago2) antibody (Millipore, Billerica, MA, USA) and anti-human CPEB2 antibody (Proteintech ...
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bioRxiv - Bioengineering 2020Quote: ... finally various concentrations of HMGB1 (1-100 ng/ml) and a HMGB1 (1000 ng)-containing AS scaffold with 10 μM of 5-bromo-2’-deoxyuridine (BrdU; Sigma, St Louise, USA) were added into 1 ml of serum-free medium and cultured for additional 24 hours ...