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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and membrane fractions were isolated using a Compartment Protein Extraction Kit (Millipore, Billerica, MA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Soluble protein was measured using a Bicinchronicinc acid kit (BCA1, Sigma-Aldrich Canada Co.). Absorbance of each reaction was measured in a spectrophotometer (Spectra Max M2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were detected with an enhanced chemiluminescence assay kit (ECL Plus, Millipore Corporation, USA). Signals were visualized using ImageLab 1.46 (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich, Pool, UK) using bovine serum albumin as standard ...
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bioRxiv - Molecular Biology 2022Quote: ... Total or nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma-Aldrich). Protein samples were electrophoresed on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2023Quote: ... Protein precipitates were solubilized in 6M urea and subsequently quantified using BCA kit (Sigma). Ten micrograms of the protein samples were reduced with 10mM dithiothreitol (DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was transferred onto a PVDF membrane (Immobilon-P) and detected using mouse anti-c-Myc antibody (Millipore Sigma) as a primary (1:5,000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated with 381.7 ng / ml mouse α-HAdV hexon protein antibody (Mab8052, Sigma-Aldrich, St. Louis, USA) and subsequently stained using 2 μg / ml goat α-mouse-AlexaFluor594 (A21203 or A32742 ...
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bioRxiv - Neuroscience 2022Quote: Mouse monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluted 1:200; cat. no. G3893; Sigma-Aldrich, MO, USA) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2019Quote: ... Expression of MxA proteins was detected by Western blot analysis using mouse ANTI-FLAG® monoclonal antibody F4042 (Sigma) and rabbit anti-beta-actin polyclonal antibody ab8227 (Abcam ...
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bioRxiv - Genetics 2020Quote: ... Proteins were detected using either secondary anti-mouse or anti-rabbit IgG coupled to peroxidase (Millipore, 1:5000 dilution), western bright ECL substrate (Advansta ...
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bioRxiv - Molecular Biology 2022Quote: ... Nanobody fusion proteins were detected using a primary anti-HA (mouse; 1:3,000, Sigma-Aldrich, St. Louis, MO, USA). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-BRI2 proteins were immunoprecipitated with anti-FLAG mouse monoclonal antibody M2 cross-linked to agarose beads (Sigma A2220); immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799) ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...
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bioRxiv - Cell Biology 2024Quote: ... using the KAPA SYBR® FAST qPCR Master Mix (2×) Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... an NPC1 domain C-targeting mAb were directly labeled with Duolink® In Situ Probemaker PLUS and Duolink® In Situ Probemaker MINUS (Sigma) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... shRNAs stably integrated in mouse and human cells were selected with 10 and 2 μg/ml puromycin (Sigma), respectively ...
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bioRxiv - Neuroscience 2020Quote: ... were incubated with secondary antibodies for 2 h: Alexa Fluor 488 goat anti-mouse IgG (1:400, Millipore), or Fluor 594 goat anti-rabbit IgG (1:400 ...
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bioRxiv - Immunology 2023Quote: ... PS Standard plates (781724; Brand) were coated with an anti-mouse IgM (Sigma; M8644; at 2 μg/ml), anti-mouse IgG1 (Biolegend ...
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bioRxiv - Synthetic Biology 2023Quote: ... and incubated for 2 h in 1:20,000 horseradish peroxidase (HRP)-conjugated goat α-mouse IgG (Sigma-Aldrich)/1% BSA/TBS ...
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bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2019Quote: ... the plasmid containing the insert was linearized and purified with Gene-Elute PCR Clean-Up Kit (Sigma). DNA concentration was measured with Qubit (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... 350 μl RLT buffer (from the AllPrep DNA/RNA Micro Kit) containing 1% β-Mercaptoethanol (Sigma-Aldrich) was added to each sample (containing one biopsy ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk in TBS-T followed by incubation in the following primary antibodies that were diluted in TBS-T containing 5% milk: mouse-anti-GFP (Sigma, 1:1000), rabbit-anti-aldolase (Mesén-Ramírez et al ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each and 100 μl of blocking solution containing goat anti-mouse IgG (Sigma 1:3,000) was added to each well and incubated at 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... the plated suspensions (containing satellite cells) were transferred to flasks coated with 1μg/cm2 mouse laminin (Sigma #CC095, St. Louis, MO, USA), which were left untouched for three days before growth media was changed ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated for 30 min at room temperature in PBS-T containing 5% milk powder and secondary antibody: peroxidase-conjugated anti-mouse IgG (1:5000) (Sigma-Aldrich, A2304). The membranes were then washed in PBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... and transferred to tissue culture dish containing M2 media (M2 Medium-with HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo, SIGMA, product #M7167) where it was incubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to primary antibody solution containing monoclonal mouse anti-vesicular glutamate transporter 1 (VGlut1) antibody (1:750; Millipore, Burlington, MA) in blocking buffer and incubated overnight at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... mid-sagittal cerebellar vibratome sections were blocked and permeabilized with 10% normal goat serum in PBS containing 0.2% Triton X-100 and immunostained with a mouse anti-Calbindin D28k antibody (Sigma-Aldrich, 1:1000) followed by incubation with Alexa Fluor 555-labeled goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... and transferred to a tissue culture dish containing M2 media (M2 Medium-with HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo, SIGMA, product #M7167) where it was incubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4 °C with BB containing a primary antibody cocktail (1:150 mouse anti-ionized calcium-binding adapter molecule 1 (Iba1; EMD-Millipore cat# MABN92+), 1:300 rabbit anti-transmembrane protein 119 (TMEM119 ...
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bioRxiv - Cell Biology 2020Quote: ... Normal mouse/rabbit IgG or RNA Polymerase II antibodies (provided by Millipore ChIP kit) were used as negative or positive control ...
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bioRxiv - Biochemistry 2020Quote: ... The mAbs were isotyped using a mouse mAb isotyping test kit (Sigma-Aldrich, USA). The icELISA described above was used to evaluate the affinity (Ka ...
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bioRxiv - Molecular Biology 2021Quote: ... Serum TSH was analyzed with a mouse pituitary magnetic bead panel kit (EMD Millipore Corp. ...
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bioRxiv - Cancer Biology 2021Quote: PLA experiments were performed using the Red Mouse/Rabbit Duolink Starter Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: The Duolink In Situ Red Starter Mouse/Rabbit kit was from Sigma (#Cat DUO92101) and used according to the manufacturer’s protocol with primary antibodies to Orai1 (mouse 1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 μL of magnetic Protein G Dynabeads were coated with 2 μg of M2 anti-FLAG antibody (F1804, Sigma) for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2019Quote: A total mass of 600μg brain protein was subjected to reduction with 10mM Tris(2-carboxyethyl)phosphine(TCEP, Sigma) at 30°C for 2hrs and then alkylation with 20mM IAA containing 100mM ABB in the dark at room temperature for 30mins ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
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bioRxiv - Pathology 2021Quote: ... Japan) were immunized (four times at 2-week intervals) with the purified protein conjugated to TiterMax Gold (Sigma-Aldrich). Rabbits were sacrificed under excess anesthesia with pentobarbital sodium (64.8 mg/kg) ...
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bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...