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Citations for Millipore Sigma :
1651 - 1700 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 1h at RT with Avidin-Biotin complex (Vector) and then with DAB/H2O2 mix (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... with/without anti-Flag antibody then incubated (1h at RT) with nanogold-coupled secondary antibody and finally fixed with 2.5% glutaraldehyde EM grade (Sigma Aldrich, #16210) for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was amplified by induction with avidin-biotin complexes (Vectastain Elite ABC-HRP (Vector, PK-6100)) for 30 min at RT and visualized with 3,3′-diaminobenzidine (Sigma, D-5637). Additionally ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were incubated at 30 °C with shaking with sample collection at 1 hour via centrifugation and resuspension in 600 μl of ~60 °C RNAzol RT (Sigma-Aldrich). After lysing cells by pipetting and vortexing in RNAzol ...
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bioRxiv - Bioengineering 2021Quote: ... The flask was then completely covered with aluminum foil and the solution was left to stir overnight (approximately 18 h) at RT when it was quenched with 18 mg hydroxylamine hydrochloride (Sigma-Aldrich). The solution was dialyzed with molecular weight cut-off membranes (SnakeSkin™ Dialysis Tubing ...
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bioRxiv - Cell Biology 2019Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Sigma-Aldrich) for 5-30 min at RT ...
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bioRxiv - Cell Biology 2019Quote: ... blocked with 10% FBS for 25 min at RT then incubated with 1:1000 TOMM20 primary antibody (Sigma-Aldrich, cat. HPA011562) diluted in 5% FBS for 1-h at 37°C ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Neuroscience 2020Quote: ... 0.5M were added to 250 ml of saline buffer] at room temperature (RT) and then with 4% paraformaldehyde (PFA; # 158127 Sigma-Aldrich) in PBS (Phosphate Buffered Saline ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell suspensions were centrifuged again (350g, 10 min, RT) and resuspended in freezing medium containing 10% DMSO (Sigma-Aldrich, Schnelldorf, Germany) in FCS (PAN-Biotech ...
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bioRxiv - Biophysics 2020Quote: The structured PDMS stamp was dried with clean dry air and inked for 45 min at room temperature (RT) with a mixture of fibronectin solution from bovine plasma (FN) (Sigma Aldrich) and labeled (cy3.5 Invitrogen labeling kit ...
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bioRxiv - Immunology 2021Quote: ... To remove red blood cell (RBC) contamination the cell suspension was incubated for 5 minutes at RT in RBC lysis buffer (Sigma-Aldrich). Finally ...
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bioRxiv - Cancer Biology 2022Quote: ... and cells were then incubated for 90 minutes at RT with 0.1% Triton X-100 containing Alexa Fluor 647-conjugated anti-puromycin antibody (1:5000; Millipore, MABE343-AF647). Cells were washed thrice with PBST for 5 minutes each ...
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bioRxiv - Cell Biology 2022Quote: ... centrifuged at 4,000 rpm for 10 min at RT and the supernatant was filtered through a 0.45 μm filter (EMD Millipore, Billerica, MA). The lentivirus-containing medium was snap frozen in 1 mL aliquots and stored at −80°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 ml lysis suspension were then incubated for 30 min at RT with 20 μl heme solution (100 mg heme (Sigma Aldrich) dissolved in 6 ml NaOH) ...
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bioRxiv - Neuroscience 2023Quote: ... BP151-500) in 1X PBS then blocked for 2 hours at RT with 2% bovine serum albumin (BSA, Sigma-Aldrich, A2153), 0.1% Triton in 1X PBS ...
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bioRxiv - Neuroscience 2023Quote: Slices were blocked for 2h at room temperature (RT) in a solution containing 3% bovine serum albumin (BSA,A7906 Sigma-Aldrich) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... 2 washes of 100% DCM (15 minutes each at RT) were performed before placing samples in dibenzyl ether (DBE, Sigma 108014) for clearing and imaging ...
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bioRxiv - Neuroscience 2022Quote: ... After incubation of primary antibodies at 4°C overnight and secondary antibodies for 1 h at RT (Alexa Flour 488 and 594) cells were embedded in Mowiol (Sigma-Aldrich) and subjected to imaging with the software Axiovision 4.8 at the microscope Axio Observer Z.1.
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bioRxiv - Molecular Biology 2022Quote: ... then alkylated for 15 min at RT in the dark with 10 mg/mL iodoacetamide (Sigma-Aldrich, St. Louis, MO, USA). The protein mixture was then digested overnight with trypsin ...
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bioRxiv - Microbiology 2023Quote: ... in PBS 1X at RT for 40 min and incubated with a blocking buffer containing 3% bovine serum albumin (BSA, Sigma Aldrich) in PBS 1X RT for 40 min ...
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bioRxiv - Biophysics 2023Quote: Immunofluorescence was performed on adhered cells after fixation with 4% PFA at room temperature (RT) for 10 min and permeabilization with 0.5% Triton® X-100 (Sigma-Aldrich) at RT for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and during the secondary antibody incubations (60’ at RT in dark) DAPI was included as a nuclear counterstain (1ug/mL; Sigma Aldrich). Directly conjugated primary antibodies were incubated as a final step prior to washing and mounting (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides were rinsed three times with PBS and blocked for 1 hour at RT in blocking buffer made with 3% BSA (Merck Millipore, 0218072801) and 0.02% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... An additional washing step was followed by a 1 h incubation at RT with the primary mouse anti-c-myc antibody (500 µl, 1:200, Sigma Aldrich). Further ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then permeabilized for 20Lmin in PBS containing 0.1% Triton X-100 and 10% goat serum (GS) at RT and immunostained with a guinea-pig polyclonal anti-vglut1 (1/4000; Millipore AB5905), a mouse monoclonal anti-BIII tubulin (1/1000 ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed thrice with 1×PBS and incubated for 15 min at RT with 0.1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, Poland). After a final three washes with 1×PBS ...
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bioRxiv - Microbiology 2023Quote: ... Incubation with primary antibodies (1 h, RT) was followed by washing and incubation with secondary antibodies and DAPI (25 µg ml-1, Sigma-Aldrich) (1 h ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed once with PBS for 10 min followed by a blocking- permeabilization step for 1 hr at RT in blocking buffer (PBS/ 5% donkey serum Bio-Rad C06SB / 0.1% Triton Sigma X100). Primary antibodies diluted in blocking buffer were then added to the cells (Table 1 ...
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bioRxiv - Genomics 2023Quote: ... we first infected MCF7 cells with EF1a-nCas9/RT lentivirus followed by treatment with puromycin (2.5 µg/ml; Sigma-Aldrich, P8833) for 2 weeks to eliminate uninfected cells ...
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bioRxiv - Cell Biology 2022Quote: ... used at 1% for 10 min at RT allowed cell permeabilization and a solution of bovine serum albumin (BSA; Sigma-Aldrich) 2% ...
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bioRxiv - Microbiology 2023Quote: ... plated on fluorodishes were incubated in 50mM NH4Cl for 5min at room temperature (RT) to quench free aldehydes and in 1X PBS containing 3% Bovine Serum Albumin (BSA, Sigma-Aldrich) for 15min at RT to block non-specific epitopes ...
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bioRxiv - Neuroscience 2023Quote: ... and 5% normal goat serum in MAB for 30 min at RT and incubated with alkaline phosphatase-conjugated anti-DIG antibody (1:10000 for oxt, 1:1000 for other genes, Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed with PBS and incubated for 15 min at RT in PBS with DAPI (1:400; Sigma-Aldrich, USA). Following washes with PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... were harvested and fixed for 1 h at RT with 2% PFA and Glutaraldehyde in 0.1M phosphate buffer pH7 and 1% sucrose (all from Sigma-Aldrich, USA). Next ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... then incubated for 30 min at RT with blocking buffer (1% Bovine Serum Albumin in 1X PBS) and further incubated for at least 1 hour at RT with rabbit anti-hACE2 antibody (SAB 3500978, Sigma-Aldrich) or rabbit anti-TMPRSS2 antibody (BS-6285R ...
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bioRxiv - Microbiology 2023Quote: ... was added for 1h at RT followed by incubation with OPD substrate revealing reaction for 10 min (Sigma-Aldrich, MO, USA). Absorbances were analyzed at 495 vs 620 nm on an ELISA plate reader (Berthold ...
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bioRxiv - Immunology 2023Quote: ... in PBS for at least 30 min at 37 °C and permeabilized with PBS containing 0.1 % Triton-X for 5 minutes and blocked with blocking solution (3 % BSA in PBS) at RT for 30 minutes (all reagents from Sigma-Aldrich). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... permeabilized with 0.25%Triton X-100/PBS for 15 min and blocked for 1 h at RT with 5% NGS (Jackson Immuno Research, 005-000-121) + 5% BSA (Sigma, A2153). The following primary antibodies were used ...
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bioRxiv - Developmental Biology 2023Quote: ... all samples were incubated with Blocking One Histo (Nacalai Tesque) for 10 min at RT and 10% normal donkey serum (Sigma-Aldrich) in PBS/0.5% Triton X-100 with 5% Blocking One Histo for 30 min at RT.
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bioRxiv - Cell Biology 2023Quote: ... the aortic tissue was incubated for at least 12 h in Roti®Histol (#6640.4, Carl Roth) at RT and embedded in paraffin (#P3558, Sigma-Aldrich).
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at RT with a rabbit polyclonal anti-pH3 antibody (1:500; Sigma; Cat#: H0412; RRID: AB_477043). Sections were then rinsed 3 times in TBS for 15 min each and incubated for 1 hour at room temperature with a Cy3-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
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bioRxiv - Neuroscience 2023Quote: ... Then, samples were incubated with streptavidin-phycoerythrin (30 min, RT) and before measurements on a Luminex MAGPIX® System (Merck-Millipore).
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated for 45 min at RT in the dark in imidazole buffer (0.1 M imidazole (Sigma-Aldrich, Cat# I5513) in PBT supplemented with 1% H2O2 ...
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bioRxiv - Cell Biology 2024Quote: ... blots were blocked 1 h at room temperature (RT) with 5% non-fat dry milk (Carl Roth, T145.2) or 5% bovine serum albumin (BSA, Sigma-Aldrich, A7906) in TBS-Tween (0.5 M Tris ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was discarded and sample was resuspended in room temperature (RT) Liver Digest Buffer [0.15 mg/mL collagenase type XI (Sigma Aldrich; C9407), 0.3 U/mL dispase (Corning ...