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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... FLAG tag (M2) and α-tubulin (T5168) from Sigma-Aldrich; βIII-tubulin from Biolegend (801201).
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag was cleaved with restriction-grade thrombin (Millipore) overnight on ice in Tris buffer supplemented with 2.5 mM CaCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... The HA tag oligonucleotide strand was synthetized by Sigma-Aldrich as two complementary single-stranded oligonucleotides (Supplementary Table 5) ...
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bioRxiv - Microbiology 2019Quote: HA Tag-FITC (7 ug/mL) (Sigma, clone HA-7)
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bioRxiv - Biophysics 2021Quote: ... The MBP tag was cleaved with Thrombin (Sigma-Aldrich, USA) at 0.4 unit per mg of protein ...
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bioRxiv - Cell Biology 2022Quote: ... then HRP tags were activated with enhanced chemiluminescence solution (Millipore). Signal was detected and imaged via ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... anti-Flag or anti-Pk (V5 Tag) antibodies (Sigma-aldrich) were used for detecting epitope-tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... which carries the N and C-terminal 6xHistidine tags (Novagen), using the Hi-Fi DNA assembly kit (New England Biosciences).
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bioRxiv - Cell Biology 2022Quote: TAG quantification was performed using Triglyceride Quantification kit (Sigma-Aldrich). For TAG quantification of whole body ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-HA-Tag (1:500, rabbit Sigma-Aldrich H3663), anti-FLAG (1:50 ...
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bioRxiv - Microbiology 2024Quote: ... rabbit polyclonal against FLAG tag at 1:500 (F7425, Millipore), rabbit polyclonal against Myc tag at 1:2000 (ab9106 ...
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bioRxiv - Microbiology 2023Quote: ... An anti-histidine tag antibody (Sigma, St. Louis, MO, USA) and serum from the immunized mice were used as positive controls ...
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bioRxiv - Microbiology 2023Quote: ... or with rabbit primary antibodies against HA-tag (Sigma-Aldrich) followed by anti-rabbit HRP-conjugated secondary antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... 100 μL of HA-tag antibody-agarose beads (Sigma-Aldrich) pre-equilibrated in extraction buffer or 5 μL of anti-LUC antibodies (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... The monoclonal anti-S-tag antibody (SAB2702227, Sigma-Aldrich®) was used ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...
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bioRxiv - Microbiology 2021Quote: Vero cells were transfected with spike protein expression constructs using the GeneJuice transfection reagent (Novagen). The indicated S-protein expression constructs (1 µg ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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Evidence for Involvement of WDPCP Gene in Alcohol Consumption, Lipid Metabolism, and Liver CirrhosisbioRxiv - Genetics 2023Quote: ... were incubated together with either 35 µL of PBST (for free glycerol measurements) or 35 µL of TAG reagent (Sigma, no. T2449, for TAG measurements) at 37 °C for 60 min ...
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bioRxiv - Developmental Biology 2020Quote: ... larval sections were counterstained with DAPI (4′,6diamidino-2-phenylindole, Sigma-Aldrich; 1:1000 μL PBS dilution) and incubated for 15 min in the dark at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and counter-stained using 4’,6-diamidino-2-phenylindole (DAPI) for nuclei (Sigma, D9542-10MG, 1:1000). Confocal images of the cortex of non-transgenic littermates and arcAβ mice were obtained using a Leica SP8 confocal microscope (Leica Microsystems GmbH ...
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bioRxiv - Cell Biology 2021Quote: ... MTs were labeled with rat monoclonal antibody YL 1/2 directed against tyrosinated -tubulin (1:1000, Millipore), or rabbit polyclonal antibody against detyrosinated -tubulin (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... or in rabbit anti-RFP (2/1000, Clinisciences, PM005) and goat anti-ChAT (1/100, Millipore, AB144P). After exposure to primary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Cell nuclei were stained with DAPI (1:1000, 4’ s-6-diamidino-2-phenylindole, Sigma Aldrich, USA) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... vibratome sections were counterstained with 1:1000 DAPI (4ʹ,6-diamidino-2-phenylindole, 1 mg/ml, Sigma) in PBS and incubated for 20 minutes in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... at 1/1000 dilution or Laurdan (#40227, 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine, Merck, Sigma-Aldrich) at 1/500 dilution for 30 min at 37°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... the heads were homogenized in 2% SDS supplemented with a protease inhibitor cocktail (1:1000; Sigma-Aldrich) using a glass rod (4°C) ...
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bioRxiv - Cell Biology 2023Quote: ... stained for 10 min with 4′,6-diamidino-2-phenylindole (DAPI; Sigma, D9542, 1:1000 in PBS), and washed extensively in PBS before mounting in RapiClear® 1.52 (SUNJin Lab ...
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bioRxiv - Microbiology 2022Quote: ... hormones were maintained in the culture medium throughout the course of each assay. 17-ß-estradiol (i. e., E2) and progesterone (i. e., P4) (both from Sigma; St. Louis, MO) were selected for use in infection studies because these hormones represent the major forms of estrogens and of progestogens (respectively ...
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bioRxiv - Physiology 2021Quote: ... The sections were further stained with hematoxylin and eosin (H&E) that was conducted on 10% formalin-fixed muscle section with Mayer’s H&E (Sigma Chemical, St. Louis, MO, USA). Immunofluorescence (IHC ...
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bioRxiv - Cell Biology 2019Quote: ... Rotenone and Williams E Media from Sigma-Aldrich (Dorset, UK). Ammonium chloride ...
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bioRxiv - Developmental Biology 2021Quote: ... Oocytes and embryos were incubated in 0.5% pronase E (Sigma) for 3-5 minutes to remove zona pellucida ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were stained with hematoxylin and eosin (H&E) (Sigma). Antigen retrieval was performed with citrate buffer pH6 ...
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bioRxiv - Neuroscience 2021Quote: ... and plated on poly-D-lysine (PDL, A003-E, Millipore) coated 10 cm dishes ...
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bioRxiv - Plant Biology 2021Quote: ... (1990) with 0.8% (w/v) agar (Type E, Sigma- Aldrich). When required ...
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bioRxiv - Microbiology 2020Quote: ... 1 mg/mL of Pronase E protease mixture (Sigma–Aldrich) was gently added directly and allowed to incubate at 37 °C (2 h) ...
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bioRxiv - Cell Biology 2022Quote: ... Isolated HFs were maintained in Williams E media (Sigma-Aldrich) supplemented with 2 mM L-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were sedimented on poly-lysine (A-003-E, SIGMA) coverslips (150µL/coverslip ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with haematoxylin and eosin (H&E; Merck + Sigma) or IHC using antibodies of interest ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with mouse anti-E-cadherin (Millipore, MAB1199) at 1:200 dilution in blocking solution at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to the Immobilon-P/E PVDF membrane (Merck Millipore), and immunodetected using the SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... followed by additional infiltration with 20 μM E-64d (Sigma) for an 8 h period ...
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bioRxiv - Neuroscience 2020Quote: ... Pronase E (Protease from Streptomyces griseus Type XIV, Sigma Aldrich) was diluted in water to a concentration of 2 mg/mL ...
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bioRxiv - Neuroscience 2020Quote: E-4031 (Alomone-Labs, IL) and TEA (Sigma-Aldrich, USA) were dissolved in 0.9% saline ...
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bioRxiv - Immunology 2020Quote: ... E-64 and 3-Methyladenine (3-MA) were from Sigma. Necrostatin-1 was from Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were stained with hematoxylin and eosin (H&E, Sigma) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and transferred to Immobilon-E PVDF Transfer membrane (Sigma, IEVH85R). Blocked the membrane with 5% skimmed milk and incubated with primary antibody i.e. ...
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bioRxiv - Molecular Biology 2022Quote: ... sectioned (3 μm sections) and stained with H&E (Sigma). LLC tumor samples and dorsal skin were embedded in 3.5- 4 % agar ...
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bioRxiv - Pathology 2024Quote: ... a 1% aqueous solution of eosin Y (Sigma E-6003) was prepared in deionized water and Harris Hematoxylin stain (Lerner Laboratories 1931382 ...
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bioRxiv - Molecular Biology 2023Quote: ... Barium – gelatin mix (E-Z-EM Canada Inc; Sigma-Aldrich) was perfused until consistent pressure matching RVSP was obtained ...