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Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... coli strain of BL21(DE3) C43 (Sigma-Aldrich) at 18°C overnight with 0.2mM Isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... coli Rosetta™(DE3)pLysS Competent Cells (Millipore) were treated with 1 mM of IPTG for 3 h ...
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bioRxiv - Immunology 2020Quote: ... coli was labeled with FITC (Isomer I, Sigma) following the manufacturer recommended procedures.
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bioRxiv - Genetics 2020Quote: LPS from Escherichia coli O111:B4 (Sigma L2630) was dissolved in sterile PBS at a concentration of 0.1mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... coli expression vector pRSFDuet1 (Novagen, Bad Soden, Germany), resulting in the plasmids pRSFDu-p31 or pRSFDu-21.2 ...
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bioRxiv - Microbiology 2022Quote: ... coli O111:B4 LPS (Sigma-Aldrich, MA, USA) was used as a positive control ...
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bioRxiv - Neuroscience 2022Quote: ... coli Rosetta using the Express Autoinduction System (Novagen). Inclusion bodies were prepared using the Bug Buster reagent (Novagen ...
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bioRxiv - Molecular Biology 2022Quote: ... and expressed in Escherichia coli Rosetta cells (Novagen). The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs ...
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bioRxiv - Microbiology 2024Quote: ... coli BL21-Rosetta(DE3)-pLysS (CmR) cells (Novagen). The expression clones were grown at 30°C in 400 ml LB medium with Cm (10 μg/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... coli BL21(DE3) pLysS cells (Novagen, Cat# 69451), extracted from inclusion bodies ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli strain TUNER cells (Sigma-Aldrich, #70623) were grown in Luria broth (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... coli chaperonin GroEL was purchased from Sigma Aldrich. For purification the lyophilized powder was dissolved at a concentration of 1 mg/mL in 1 mL of reconstitution buffer (20 mM tris acetate ...
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bioRxiv - Microbiology 2024Quote: ... coli thioredoxin 1 (TrxA; Sigma-Aldrich cat. # T0910), 0.1 mg ml-1 bovine serum albumin ...
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bioRxiv - Microbiology 2024Quote: ... coli strains were cultured in LB broth (Sigma), using the appropriate antibiotics.
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) cells (Novagen, Merck, Darmstadt, Germany). TDP-43 protein was purified to homogeneity by nickel affinity chromatography as described by Vega et al ...
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bioRxiv - Immunology 2022Quote: ... coli (O111:B4, Sigma-Aldrich, St. Louis, USA), LTA (Sigma-Aldrich) ...
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bioRxiv - Physiology 2023Quote: ... before adding LPS from Escherichia coli (Sigma Alrich) at 10ng/ml for 20 hours.
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bioRxiv - Biochemistry 2022Quote: ... coli NovaBlue GigaSingles™ Competent Cells (Novagen, Germany) and plated on LB agar with 100 μg/ml ampicillin ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells (Novagen, Merck, Darmstadt, Germany) and purified by high-performance liquid chromatography on a C4 reverse-phase column (Shimadzu ...
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bioRxiv - Microbiology 2022Quote: ... coli MRE 600 (Cat No: 10109541001; Millipore Sigma), 15% formamide (Cat ...
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bioRxiv - Microbiology 2022Quote: ... coli MRE600 (TRNAMRE-RO) was from Sigma Aldrich. Ribonucleoside-vanadyl complex (VRC ...
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bioRxiv - Immunology 2022Quote: ... +/- LPS from Escherichia coli 0111:B4 (Sigma-Aldrich) +/- 100 ng of rIL-6 (PeproTech ...
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bioRxiv - Microbiology 2023Quote: ... coli EH 100 rough LPS Ra mutant (Sigma). The MIC concentrations of attacins were preincubated with increasing concentrations of LPS in CAMH ...
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bioRxiv - Neuroscience 2023Quote: ... coli BL21 (DE3) cells (Cat No. 69450, Novagen) with the pET29b plasmids generated above ...
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bioRxiv - Microbiology 2023Quote: ... coli strains were grown in Luria-Bertani (Sigma). The following antibiotics were added to the medium where specified ...
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bioRxiv - Neuroscience 2023Quote: Lipopolysaccharide (LPS) from Escherichia coli (L3137, Sigma-Aldrich), (−)-JQ1 (SML1525 ...
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bioRxiv - Immunology 2023Quote: ... coli HT115 and contained 1 mM IPTG (Sigma) to induce expression of dsRNA ...
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bioRxiv - Microbiology 2023Quote: ... and Escherichia coli BL21(DE3) Rosetta™ (Novagen) for heterologous protein production ...
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bioRxiv - Cancer Biology 2023Quote: ... coli tRNA (Sigma Aldrich, Merck cat. no. 10109541001)/0.02% w/v bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: Lipopolysaccharide from Escherichia coli O55:B5 (Sigma, L2880) was dissolved in sterile PBS at 0.5mg/mL ...
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bioRxiv - Neuroscience 2024Quote: LPS endotoxin (Escherichia coli O111:B4 (Sigma L4391) was prepared as a stock solution at 2 mg/ml in normal saline (0.9% w/v) ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) cells were purchased from Novagen-EMD4Biosciences (Merck Chemicals ...
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bioRxiv - Immunology 2024Quote: ... coli O55:B5 (Millipore Sigma, St. Louis, MO) and treated i.p ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Lipopolysaccharide (LPS, Escherichia coli O111:B4, Sigma Aldrich) was injected intra-hippocampally at a concentration of 4µg/µl ...
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bioRxiv - Biophysics 2020Quote: The human wt-PD-1 (33 – 150 aa, C93S mutation) and mutants (N66A, Y68A, E135A and N66AY68A) were cloned into pET24d (Novagen) while recombinant PD-L1 (18 – 134 aa ...
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bioRxiv - Cell Biology 2021Quote: ... The sequence encoding Bro1ΔPRR (aa 1-709) was amplified from pGO216 and cloned into BamH1 and SalI sites of pET28b (Novagen, Germany) and pGST-parallel1 for use generating and purifying the anti-Bro1 sera ...
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bioRxiv - Cell Biology 2021Quote: ... The aorta was divided into aortic arch (AA) and thoracic aorta (TA) regions and cut into 1 mm pieces and digested with Elastase type III (Sigma) at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... selective astrocytic toxin L-α-aminoadipate (L-AA) as well as Gabapentin were purchased from Sigma-Aldrich (St. Louis, MO). Caspase-1 siRNA was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2022Quote: ... After it was fully thawed the buffer was mixed by pipetting and then filtered with a 0.8 um syringe filter (Millex-AA, SLAA033DD, Millipore Sigma) to remove any cells or large cellular debris ...
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bioRxiv - Bioengineering 2022Quote: ... the PEGDA-AA hydrogels were functionalized with collagen type I (10% w/v or 0.4 mg mL-1, Sigma-Aldrich) via N – (3-Dimethylaminopropyl)-N ′ - ethylcarbodiimide (EDC)/N-Hydroxysuccinimide (NHS ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at room temperature with an antibody against mHTT protein (1-82 aa, Millipore, MAB5492 1:500), and either NeuN (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... and transcription factor SREBP1a (aa 1-50) were generated by inserting the corresponding cDNA fragments into pGEX4T-3 vector (Sigma). V5-tagged full-length and N-terminal truncation (Δ302 ...
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bioRxiv - Plant Biology 2022Quote: ... the coding sequence of GRP7 or its mutated variants plus the GFP tag connected by a short linker (7 amino acids [aa]) or GFP alone were cloned into the EcoRI and BamHI restriction site of the pET32a expression vector (Novagen) containing a thioredoxin (Trx ...
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bioRxiv - Cell Biology 2023Quote: ... 200 µM of OA and 400 µM of DHA and AA were dissolved in filtered (0.2 µM Minisart filter; Sigma-Aldrich) RPMI media containing 5% FBS and 2% w/v bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: Polyclonal antibody against the C-terminus of mouse SETX-C were generated by immunizing rabbits. Mouse cDNA encoding Setx (aa. 1900-2701) was subcloned into pET15b (Novagen) and transformed to E.coli strain BL21-CodonPlus(DE3)-RIPL (Agilent) ...
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bioRxiv - Genetics 2024Quote: ... for tracing CTCF proteins with FLAG-specific antibodies because deletions of endogenous CTCF C-terminal fragment removed the epitope (659-675 AAs) for CTCF antibodies (Millipore). In addition ...
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bioRxiv - Cell Biology 2021Quote: ... and primary mouse monoclonal anti-□-H2AX (1:1200, Sigma-Aldrich, cat. number 05-636). 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Bioengineering 2021Quote: ... peptides were dialyzed with 500 - 1200 MWCO dialysis tubing (Sigma-Aldrich, St. Louis, MO) against DI water ...
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bioRxiv - Biochemistry 2022Quote: ... GST tag was then cleaved off using preScission protease (Sigma) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... HSV tag (Sigma-Aldrich H6030, 1:1,000 or 1:2,000), Rpb1-Core (Y80 Santa Cruz ...