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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The β-Spectrin-His construct was transformed into BL21(DE3) cells (Novagen) and induced for protein expression with 0.5mM IPTG (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Pre-cleared supernatant was mixed with Ni-NTA His-Bind Resin (Novagen) (Pajenda et al. ...
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bioRxiv - Biochemistry 2022Quote: ... His6-tagged proteins were purified with Ni-NTA His-Bind Superflow (Novagen) and dialyzed against PBS using a Pur-A-Lyzer™ Midi Dialysis Kit (Merck).
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bioRxiv - Immunology 2020Quote: ... supplemented with 15% FBS HI (Life Tehnologies) + 1% Pen/Strept (Sigma-Aldrich), 1% L-glutamine (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification of rcCBP2 was performed by HIS-Select HF Nickel Affinity (Sigma) and gel permeation chromatography on Superdex 75 column (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes followed by anti-his antibody (Sigma, Cat. No. 11922416001) for another 10 mins ...
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bioRxiv - Microbiology 2021Quote: ... The crude lysate rocked with HIS-select nickel affinity gel resin (Sigma) for 45 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Immunology 2024Quote: ... and 5% Fetal bovine serum (FBS; Sigma Heat Inactivated, US/HI origin). PBMCs were cultured overnight in chemotaxis media containing RPMI and 1% ultrapure BSA prior to use in assays.
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich) at 37°C ...
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-His antibody (Sigma, USA) followed by 1:20000 anti-mouse antibody (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
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bioRxiv - Cell Biology 2024Quote: ... another membrane was incubated with anti-His antibody (05-949, Millipore, USA) at 4°C for 12 h and then incubated with an IRDye 800 secondary antibody for 1 h ...
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bioRxiv - Immunology 2019Quote: ... Sections were stained with hematoxylin and eosin (H&E; Sigma-Aldrich) and for pancreatic tissue parallel sections stained for insulin (purified rabbit anti-mouse insulin polyclonal antibody ...
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bioRxiv - Neuroscience 2020Quote: ... 1 ml 10 mM EDTA (final: 0.1 mM, Sigma, E-6511), 1 ml 1 M HEPES (final ...
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bioRxiv - Plant Biology 2020Quote: ... 0.8% (w/v) type E agar (Sigma Aldrich, St. Louis, MO), and pH adjusted to 5.7 using 1 M NaOH and 1 M HCl ...
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bioRxiv - Microbiology 2021Quote: ... cells were sedimented on poly-D-lysine (A-003-E, Sigma) coverslips (150 μL/coverslip ...
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bioRxiv - Developmental Biology 2022Quote: ... and then stained with Hematoxylin and Eosin (H&E, Sigma-Aldrich) or Periodic acid-Schiff (PAS ...
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bioRxiv - Biochemistry 2022Quote: ... and the cysteine protease inhibitor E-64 (200 μM; Sigma-Aldrich), were added to the reaction ...
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bioRxiv - Neuroscience 2021Quote: ... samples were colored with blue evans (Sigma, cat. n. E-2129) 0.02% in PBS for 15 minutes) ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were sedimented on poly-D-lysine (A-003-E, SIGMA) coverslips (150 μL/coverslip ...
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bioRxiv - Physiology 2022Quote: ... Slides were stained with hematoxylin and eosin (H&E; Sigma-Aldrich) and then digitized under bright light using a Hamamatsu NanoZoomer (Hamamatsu Photonics) ...
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bioRxiv - Immunology 2019Quote: ... Sections were stained with hematoxylin and eosin (H&E; Sigma-Aldrich) or for insulin ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were stained with Harris’ hematoxylin and eosin (H&E) (Sigma). For immunostaining embryos from Hoxb1-/- or Hoxb1GoF;Mef2c-Cre and littermate controls were fixed at 4°C for 20min in 4% paraformaldehyde ...
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bioRxiv - Neuroscience 2020Quote: ... cells were plated on poly-D-lysine (PDL, #A003-E, Millipore)-coated 10 cm dishes (#130182 ...
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bioRxiv - Genetics 2021Quote: ... Proteins were then transfered to an Immobilon-E PVDF membrane (Millipore), which was then washed with 1× PBS containing 0.05% Tween 20 (three washes ...
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bioRxiv - Immunology 2020Quote: ... After paraffin embedding the sections were stained H&E (Sigma, USA) and processed at Leica DM4000 B microscope (Leica Cambridge Ltd ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The hepatocyte overlay and maintenance medium contains Williams’ Medium E (Sigma) with final concentrations of 1% Pen/Strep (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: ... solutions (H&E) and Masson’s trichrome stain (HT15-1KT, Sigma Aldrich) were used to assess cellularity and capsule thickness ...
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bioRxiv - Cancer Biology 2022Quote: ... Subsequently H&E staining (#MHS16, #HT110116 Millipore Sigma, Burlington MA, USA) was performed according to Visium technical parameters (CG000409 ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Cell Biology 2023Quote: ... All PCLSs were cultured in Williams medium E (W4128; Sigma-Aldrich), supplemented with 1% penicillin/streptomycin and l-glutamine ...
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bioRxiv - Bioengineering 2023Quote: ... H&E staining was performed with Hematoxylin (Sigma, Saint Louis, USA) and Eosin Y (Merck ...
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bioRxiv - Bioengineering 2023Quote: ... All slices were cultured in 1mL Williams’ Medium E (Sigma Aldrich) with 2% FBS ...
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bioRxiv - Pathology 2023Quote: Hair follicle parameters were stained with H&E (H3136 Sigma-Aldrich Hematoxylin and E4009 Sigma-Aldrich Eosin Y) ...
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bioRxiv - Molecular Biology 2023Quote: ... Then NPCs were plated on Poly_D_lysine (PDL, Millipore A-003-E) and Mouse laminin (sigma L2020-1MG ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... stained with hematoxylin-eosin (H&E) (MHS32, Sigma-Aldrich, Louis, MO) and examined under a light microscope (Olympus BH-2 ...
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bioRxiv - Bioengineering 2023Quote: ... 35 µL protease inhibitor cocktail (1 µM E-64, Sigma Aldrich), and 2.5 µL lysozyme (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: ... DUSP6 inhibitor (E/Z)-BCI hydrochloride 100ng/ml (Sigma-Aldrich, #B4313), T3 40ng/ml (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 nM 17β-estradiol (E2, E; Sigma, St. Louis, MO, USA), 0.2 μg/mL recombinant human relaxin-2 in 0.1% BSA-PBS (Rln ...
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bioRxiv - Biochemistry 2021Quote: ... The MERS-CoV peptide was labeled with sub-stoichiometric FITC (Sigma-Aldrich, St. Louis, Missouri) in a basic HEPES buffer (pH 8.2 ...
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bioRxiv - Immunology 2021Quote: LPS from Escherichia coli serotype 055:B5 (Sigma-Aldrich, L2880) was used ...