Labshake search
Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
-
bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
-
bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
-
bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
-
bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
-
bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
-
bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
-
bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
-
bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
-
bioRxiv - Neuroscience 2021Quote: ... mouse α-tubulin clone B-5-1-2 (1:1000; Sigma Aldrich, San Luis, MO, U.S.). For immunofluorescence primary antibodies were labeled with Alexa-conjugated secondary antibodies Alexa 488 ...
-
bioRxiv - Immunology 2022Quote: ... The slides were then stained with 1/1000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 minutes at room temperature and washed twice for 5 minutes in PBS ...
-
bioRxiv - Immunology 2021Quote: ... for 2 hours at room temperature and incubated with primary antibodies: GFAP (1:1000, Sigma-Aldrich), IBA1 (1:500 ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell nuclei were stained with 10.9 μM 4′,6-diamidino-2-phenylindole (DAPI, 1:1000 Sigma). Coverslips were mounted in ProlongGold (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich, 1:1000).
-
bioRxiv - Neuroscience 2023Quote: ... slides were incubated in 4′,6-diamidino-2-phenylindole (1:1000, DAPI, Sigma-Aldrich, Cat # D9542) solution for 10 mins ...
-
bioRxiv - Neuroscience 2023Quote: ... for 2 h in the dark at RT and counterstained with DAPI (1:1000, Sigma, T7024). Finally ...
-
bioRxiv - Cell Biology 2023Quote: ... 2% BSA in PBS for 15 minutes and stained with DAPI (Sigma-Aldrich; #MBD0015; 1:1000) for 5 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... for 10 min before being incubated with 1:1000 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) and 1:200 Phalloidin Alexa-647 (Stratech ...
-
bioRxiv - Microbiology 2021Quote: ... Escherichia coli K-12 (ATCC 19020) and Escherichia coli EK-19 were inoculated in sterile Luria Broth (LB, L3522, Sigma) and incubated at 37 °C overnight with a shaking speed of 200 rpm ...
-
bioRxiv - Microbiology 2021Quote: BHK-SARS-2R_GFP_NeoR_NL cells were trypsinized and washed twice with DPBS (Sigma-Aldrich) and once with sort buffer (PBS supplemented with 0.3 % BSA ...
-
bioRxiv - Cell Biology 2024Quote: ... and incubated sequentially with primary antibodies anti-pSer5 CTD S2 (Millipore, 04-1571, 1:500 dilution), anti-pSer5 CTD S5 (Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... p15A-CmR backbone plasmids (see Table S2) were maintained using 30 µg/mL chloramphenicol (Sigma-Aldrich) in plates ...
-
bioRxiv - Neuroscience 2024Quote: ... Total supernatant (S1 + S2) was passed through a 10 μm hydrophobic PTFE filter (LCWP02500, Merck Millipore) and centrifuged for 1 min at 4,000 g ...
-
bioRxiv - Biophysics 2021Quote: ... coli expression plasmid vector pET-28b (Novagen, MA) was used to construct all the gp17 WT and mutant clones (37) ...
-
bioRxiv - Immunology 2019Quote: ... coli 0127:B8 were purchased from Sigma-Aldrich, St ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lipopolysaccharides from Escherichia coli O55:B5 (Sigma L6529) were diluted to 1mM in phosphate-buffered saline ...
-
bioRxiv - Biochemistry 2019Quote: ... coli strain from the pRSFDuet-1 vector (Novagen). The purification protocol entailed Ni-NTA agarose chromatography (Qiagen) ...
-
bioRxiv - Immunology 2019Quote: ... and Escherichia coli lipopolysaccharide (LPS) from Sigma-Aldrich. Collagenase type I was from Worthington Biochemical Corporation ...
-
bioRxiv - Biochemistry 2020Quote: ... Escherichia coli BL21(DE3) competent cells (Novagen®) transformed with each of the plasmids (I2A ...
-
bioRxiv - Biochemistry 2020Quote: ... coli cells for protein expression (Novagen, chloramphenicol resistance).
-
bioRxiv - Biophysics 2021Quote: ... coli strains for expression were purchased from Novagen. E ...
-
bioRxiv - Neuroscience 2020Quote: ... coli BL21(DE3) cells (Sigma, cat. no. CMC0014) transformed with a pET19n vector containing 2N4R Tau were induced by the addition of 1 mM IPTG and harvested by centrifugation ...
-
bioRxiv - Biochemistry 2021Quote: ... coli BL21 (DE3) cells (Novagen, Merck, Darmstadt, Germany). PrP proteins were purified by high-performance liquid chromatography on a C4 reverse-phase column (Shimadzu ...
-
bioRxiv - Plant Biology 2021Quote: ... coli strain BL21(DE3)pLysS (Novagen, now Merck) and expression plasmid carrying AHK5HK-RD gene into ER2566 (NEB ...
-
bioRxiv - Plant Biology 2021Quote: ... coli strain OverExpress™C43 (DE3) (Sigma-Aldrich) was used to express the full-length constructs of Vmp1 and Vmp2 ...
-
bioRxiv - Cell Biology 2021Quote: ... coli BL21 (Novagen/ Merck Millipore, Burlington, Massachusetts, USA) by the addition of 0.2 mM IPTG to an overnight culture of bacteria in LB medium at 18°C ...
-
bioRxiv - Cell Biology 2021Quote: LPS (from Escherichia coli strain 0127:B8, Sigma) suspended in PBS was injected intraperitoneally into mice at 0.6 mg per 30 g mouse body weight for inflammatory cytokine ...
-
bioRxiv - Neuroscience 2021Quote: LPS (from Escherichia coli 0111: B4 [Sigma-Aldrich]) dissolved in 0.9% NaCl was intraperitoneally injected to pregnant rats at a dose of 100 mg/kg/ml on gestational days (GD ...
-
bioRxiv - Cell Biology 2021Quote: ... Coli cells containing tRNA’s for “universal” translation” (Novagen) maintained under chloramphenicol ...
-
bioRxiv - Immunology 2020Quote: Escherichia coli strain O111:B4 LPS (Sigma-Aldrich) and enterotoxigenic Escherichia coli K88 (ETEC ...
-
bioRxiv - Systems Biology 2021Quote: ... coli Origami and Origami B (Novagen Inc., USA). These strains carrying the recombinant plasmids were propagated in LB containing ampicillin (100 μg/ml) ...
-
bioRxiv - Biophysics 2022Quote: ... coli BL21 Rosetta2 (DE3) cells (Novagen, Wisconsin, USA). Cells were lysed in 20 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2020Quote: Escherichia coli BL21 Rosetta™(DE3) (Merck/Novagen) cells harbouring the co-expression vectors were grown at 37 °C in LB medium (Lysogeny-Broth ...
-
bioRxiv - Bioengineering 2020Quote: ... coli BL21(DE3) (Novagen, Merck KGaA, Darmstadt, Germany), B ...
-
bioRxiv - Physiology 2020Quote: ... coli O111:B4 (1 mg/kg; Sigma-Aldrich) were intraperitoneally injected 5 min after a CNO injection ...
-
bioRxiv - Physiology 2020Quote: ... coli O111:B4 (6 mg/kg; Sigma-Aldrich) and either saline or 1% 2MT (200 µl ...
-
bioRxiv - Biochemistry 2021Quote: ... coli BL21 (DE3) cells (Novagen, Merck, Darmstadt, Germany). SOD1 protein was purified to homogeneity by Q-Sepharose chromatography as describe by Chattopadhyay et al.26 and Xu et al.6 ...