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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... rabbit αMyc Tag IgG (1:3000; #06-549, Millipore), rabbit αHSF2BP R2 (1:2000 ...
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bioRxiv - Biochemistry 2022Quote: ... and S-tag from MBP-enhanced vector pTriEx4 (Novagen); the second insert contained CL Affinity Tag followed by a 90bp linker taken from pl21 plasmid (TriAltus) ...
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bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
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bioRxiv - Cell Biology 2023Quote: ... TAMRA-alkyne tag (1:5000; Sigma Aldrich, Cat# 900932), TCEP solution (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Antibody for Flag-Tag (A8592) was ordered from Sigma.
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bioRxiv - Cell Biology 2024Quote: ... Mouse anti–S-tag was from Novagen (Gibbstown, NJ). Rabbit anti-GFP was from Santa Cruz Biotech ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tag mouse mAb was purchased from Sigma (USA); goat anti-rabbit IgG H&L/HRP was purchased from Bioss (China) ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-T7 tag-specific monoclonal antibodies were from Novagen.
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-Flag tag (Millipore Sigma, F3165, 1:5000). Membranes were scanned on a LI-COR Odyssey Imager ...
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bioRxiv - Immunology 2023Quote: PLGA-PEG-PLGA (1000-1000-1000) (PPP, Sigma Aldrich) were purchased and dissolved in 1X phosphate buffered saline at 4 °C overnight by gentle vortexing at 200 mg/mL concentration ...
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301, Goat α-mouse HRP 1:1000– Sigma Aldrich/GERPN4401), revealed using ECL reagent (BioRad ...
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bioRxiv - Neuroscience 2020Quote: ... the complete OPC medium was removed and replaced with OPC differentiation medium [complete OPC medium lacking PDGF-AA and containing 4μg/ml triiodothyronine (Sigma)] for 4 days before cells were fixed by exposure to 4% PFA (w/v ...
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bioRxiv - Developmental Biology 2019Quote: Embryos at all stages were cultured on 35mm Petri dishes (Falcon) within microdrops of 10-15µl of Potassium Simplex Optimized Media with amino acids (KSOM-AA, Millipore), at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were dissociated with Accutase and replated onto polyornithine/fibronectin/laminin-coated coverslips in neural differentiation medium (NDM, Neurobasal plus, 1x B27 supplement, 1x GlutaMAX, 1x penicillin streptomycin) plus ascorbic acid (AA, 0.2 mM, Sigma), and DAPT (1µM ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Cre reverse primer 5’-GGT TTC CCG CAG AAC CTG AA-3’ (both primers were purchased from Sigma-Aldrich).
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bioRxiv - Biophysics 2022Quote: Short (208 aa to end) isoform of Drosophila melanogaster spastin was expressed in bacteria (Rosetta-DE3-competent cells; Novagen), purified and stored as previously described (56).
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bioRxiv - Plant Biology 2022Quote: ... the coding sequence for the mature STIC2 protein (aa 49-182) was cloned into BamHI/SalI linearized pETDuet1 (Novagen) and pGEX4T3 (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNAs encoding human AMPKα1-KD (aa 27-290) (ref. 164) and rat CaMKK2-KD42 were inserted into the pET-28a (Novagen) vectors for expressing His-tagged recombinant proteins as described previously ...
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bioRxiv - Cell Biology 2023Quote: ... was induced by an intraperitoneal injection of three doses of AA (5 mg/kg body weight, Sigma, cat# A5512) dissolved in sterile saline ...
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bioRxiv - Bioengineering 2024Quote: One gram of mucin glycoprotein from bovine submaxillary gland was treated with 1U neuraminidase from Vibrio cholerae (Sigma-Aldrich, Saint Louis, MO, USA) in sodium acetate buffer 0.05 M (pH 5 ...
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bioRxiv - Microbiology 2023Quote: ... we also stained cells expressing wild-type and mutant EEEV glycoproteins with a mouse anti-EEEV E1 antibody clone 1A4B.6 (Sigma-Aldrich Cat#: MAB8754) or a mouse IgG2b isotype control (BD Biosciences Cat# ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Bioengineering 2020Quote: ... an Entactin-collagen IV-laminin (E-C-L, Millipore) solution (10 μg/mL in sterile serum-free medium ...
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bioRxiv - Cell Biology 2020Quote: ... coated with poly-D-lysine (Sigma; A-003-E) at a density of 0.25×106 cells/mL ...
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bioRxiv - Cell Biology 2020Quote: ... with 0.25% % fetal bovin e serum (FBS, Sigma-Aldrich). Cells were then directly fixed for 20 min at RT with 4% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Cancer Biology 2021Quote: ... and 20 ng/mL rEGF (Sigma Aldrich, E-9644) was added into each well ...
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bioRxiv - Genetics 2022Quote: ... a γ-secretase inhibitor Compound E (0.2µM, EMD Millipore) was added to the cells to inhibit cell division ...
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bioRxiv - Developmental Biology 2019Quote: ... rat anti-E-cadherin (Cdh1) (1:300 dilution, Sigma). Secondary antibodies against sheep ...
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bioRxiv - Cell Biology 2021Quote: ... stained with hematoxylin and eosin (H&E; Sigma-Aldrich), or stained with PAS reagent ...
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bioRxiv - Systems Biology 2021Quote: ... 20 ng/ml Epidermal Growth Factor (Sigma, #E-9644), 100 ng/ml cholera toxin (Sigma ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... or the e-cigarette vehicle (propylene glycol; Sigma-Aldrich) daily from GD 5-20 during a 40-min vapor inhalation session (2 L/min airflow ...
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bioRxiv - Immunology 2021Quote: ... and/or 2.5 μg/ml E-cadherin-Fc (Sigma). IELs were plated in RPMI-T medium containing 2 μg/ml PerCP-e710 conjugated anti-CD107a (1D4B ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Complete hepatocyte seeding medium contains Williams’ Medium E (Sigma) with final concentrations of 1% Pen/Strep (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1 μM Purmorphamine and 0.1 μM Compound E (Millipore) for 6 days to mature into CHAT+ motor neurons.
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bioRxiv - Cell Biology 2022Quote: ATDC5 cells (Sigma-Aldrich #99072806 supplied by E-ACC) were cultured in DMEM/F12 (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... 17-β-estradiol (E2) (Cat# E-060, Sigma Aldrich) was used in all assays at a physiologic concentration of 1 nM/mL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... H&E staining was performed using Hematoxylin (Sigma-Aldrich) and Eosin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... the culture medium was HFH: Williams’ Media E (Sigma) supplemented with nicotinamide (12 mM) ...
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bioRxiv - Immunology 2023Quote: ... or 100 ng/mL LPS (E. coli:B4, Sigma-Aldrich). Choline-free DMEM was formulated by preparing nutrient-deficient DMEM (US Biologicals ...
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bioRxiv - Cancer Biology 2023Quote: ... monoclonal rat anti-E-cadherin (1:300, Sigma-Aldrich), mouse anti-p120-catenin (1:400 ...
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bioRxiv - Molecular Biology 2023Quote: ... For ethylene glycol tetratacetic acid (EGTA; Sigma E-3889) treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... For hematoxylin and eosin (H&E) staining (Sigma-Aldrich), standard procedures were followed ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...