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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... coli Rosetta-gami B(DE3)pLysS (Novagen, Sigma-Aldrich) and cultures in LB (Luria-Bertani ...
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bioRxiv - Plant Biology 2023Quote: ... coli Rosetta-gami B(DE3)pLysS (Novagen, Sigma-Aldrich) and cultures in LB (Luria-Bertani ...
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bioRxiv - Neuroscience 2023Quote: ... coli strain O127:B8 (100 ng/mL; Sigma Aldrich), Pam3CSK4 (100 ng/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli 0111:B4 (L5293) were purchased from Sigma-Aldrich. The ReLPS was purchased from Avanti Polar Lipids (Kdo2-Lipid A (KLA) ...
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bioRxiv - Biophysics 2023Quote: ... coli Rosetta (DE3) pLysS strain (Novagen®, Darmstadt, Germany) and selected on kanamycin (25 µg/ml ...
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bioRxiv - Biophysics 2023Quote: ... coli cells in Overnight Express Instant TB medium (Novagen). Proteins were purified via immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Microbiology 2024Quote: ... coli BL21(DE3) pLysS competent cells (Novagen, Darmstadt, Germany) containing the pET28-based expression system were cultured in LB at 37 °C to an OD600 of 0.5 ...
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bioRxiv - Biophysics 2024Quote: ... coli strain BL21(DE3) (Millipore Sigma, Burlington, MA, USA), followed by incubation with shaking at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: LPS from escherichia coli O55:B5 (Sigma-Aldrich, L6529) was diluted to 2mg/ml in PBS and stored at -80°C ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP tag was detected with peroxide–anti-peroxide complexes (P1291; Sigma). Detection was performed using the enhanced chemiluminescence procedure (ECL kit).
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bioRxiv - Developmental Biology 2019Quote: ... incubated with antibodies directed either against HA-tag (Sigma, 1:200) or VEGFC (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... anti-MYC (for MYC tag) (Sigma, St. Louis, MO, cat # C3956).
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bioRxiv - Plant Biology 2021Quote: ... and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... The GST tag was cleaved with thrombin (commercial thrombin, Sigma Aldrich), at 200 units per mL for 2h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Mouse anti-histidine tag 1:200× (Sigma-Aldrich, St. Louis, MO) and HRP-conjugated goat anti-mouse IgG 1:1,000× (Seracare Life Sci. ...
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bioRxiv - Biochemistry 2022Quote: ... the anti-Myc tag antibody clone 4A6 (Millipore, cat# 05-724), and the anti-RNA polymerase II antibody clone CTD4H8 (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The forward primer contained a 6-FAM (6-carboxyfluorescein) tag (Sigma). EMSA reaction mixtures (25 μL volume ...
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bioRxiv - Molecular Biology 2024Quote: ... and validated by Western Blotting for 3xFLAG tag (Antibody M2, Sigma).
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG tag (Merck, Darmstadt, Germany /Sigma Aldrich, St. Louis, MO, USA), Spot tag (ChromoTek ...
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... and a mouse monoclonal anti-FLAG epitope-tag antibody (Sigma #F7425). For the detection of endogenous Pdlim5 ...
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...
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bioRxiv - Microbiology 2023Quote: ... rabbit polyclonal anti-FLAG-tag (#F7425, Sigma-Aldrich, dilution 1:1,000), rabbit polyclonal anti-EGFP (#2555 ...
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bioRxiv - Neuroscience 2023Quote: The StrepII–GFP tag was cleaved by HRV 3C protease (Novagen) added at 10 U/mg of BmGr9 overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Flag tag (F3165) and C14ORF43 (HPA003111 for WB) from Sigma-Aldrich. Monoclonal antibodies against TFII-D subunits were generous gifts from Dr ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies against the VSV-G epitope tag (Sigma, batch # 0000143676) were used at 1:5,000 overnight at 4°C and primary antibodies against LasR (25 ...
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG-tag mAb (F1804-200UG) was purchased from Sigma-Aldrich, St ...
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bioRxiv - Immunology 2022Quote: ... Spike-transfected CEM NKR-Luc cells were stained with PKH26 kit according the manufacturers protocol (Sigma) prior to performing the ADCC assay ...
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bioRxiv - Biophysics 2020Quote: ... Cells were treated with a spike of 25 nM Tetramethylrhodamine ethyl ester perchlorate (TMRE, Sigma 87917) for 30 minutes before imaging ...
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bioRxiv - Genomics 2022Quote: ... These 16 spike-in oligomers were purchased in two separate batches (Table S1) from Sigma Aldrich and resuspended in 10 mM Tris (pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1:1000 in blocking buffer) and 0.5–0.8 μg/mL DAPI (4,6-diamidino-2-phenylindole, Sigma-Aldrich, D9542) for 1 h at room temperature ...
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bioRxiv - Physiology 2022Quote: ... a 4 uL suspension of 2 μm red-fluorescent polystyrene microspheres (Sigma-Aldrich; 1:1000 dilution in PBS) was apically applied to native mucus ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The pellets containing crude nuclei were resuspended in 1000 μl Staining Buffer (PBS, 2% BSA (Sigma cat. # SRE0036), 80 U/ml Protector RNase Inhibitor) ...
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bioRxiv - Physiology 2023Quote: ... the gonads were incubated overnight in the dark at 4 °C with secondary goat anti-rabbit IRDye 680RD-conjugated antibody (LiCor) 1:1000 in PBST/0.1% BSA and 1 ng/µl 4,6-diamidine-2-phenylindole (DAPI, Sigma). The gonads were mounted on 2% agarose pads in Fluoromount-G (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... b-mercaptoethanol and 1000 U ml-1 LIF) and 2 ug ml-1 doxycycline (cat. 33429 Sigma-Aldrich). MEFs were reprogrammed in the presence of doxycycline for 12 days and without doxycycline for the last 5 days ...
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bioRxiv - Neuroscience 2019Quote: Adult brain dissection was performed in ice-cold S2 cell culture medium (Schneider’s Insect Medium, Sigma Aldrich, #S0146) and brains were fixed with 4% PFA (v/w ...
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bioRxiv - Cell Biology 2021Quote: WT S2 cells (1.5×106) were plated on coverslips and permeabilized with 10 μg/ml digitonin (Sigma- Aldrich) in KRB for 2h at 26°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The Drosophila S2 cells were maintained in HyClone™ SFX-Insect Cell Culture Media containing 10% FBS (Sigma).
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bioRxiv - Neuroscience 2022Quote: ... Quantitative PCR was performed for the amplification of cDNAs using the appropriate primers (Sigma-Aldrich, see Table S2) and the GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Neuroscience 2019Quote: Adult brains dissection was performed in ice-cold S2 cell culture medium (Schneider’s Insect Medium, Sigma Aldrich, #S0146) and brains were fixed with 4% PFA (v/w ...
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bioRxiv - Genetics 2021Quote: ... Drosophila S2R+ (CVCL_Z831, DGRC Cat # 150), S2 DGRC (CVCL_TZ72, DGRC Cat # 6) cells were grown in M3 media (Sigma) containing Bactopetone ...
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bioRxiv - Developmental Biology 2021Quote: Total mRNA from ds- egfp and ds-hyx-treated S2 cells were extracted using TRI Reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Two independent guide RNAs for ITGA5 (Table S2) from the Brunello sgRNA library (49) were synthesized (Sigma- Aldrich) and cloned in to the pLentiCRISPRv2-blast plasmid (#98293 ...
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bioRxiv - Neuroscience 2023Quote: ... and dissected in cold S2 media on a 9-well glass plate coated with silicone (Sylgard 184, Sigma) using forceps (Dumont #5 ...
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bioRxiv - Cell Biology 2019Quote: ... Rabbit polyclonal antibody against EphB1 sequence (AA 528-541, DDDYKSELREQLPL) (anti-EphB1 pAb) was custom produced by Sigma-Aldrich (St. Louis, MO). Anti-EphB1 mouse monoclonal against EphB1 sequence (AA 528-541 ...
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bioRxiv - Cell Biology 2019Quote: The intracellular ATP content in the HGs was measured with an Adenosine-5’-triphosphate bioluminescent assay kit (FL-AA, Sigma Aldrich Inc.). Bioluminescent luciferase-luciferin reactions provide the basis of simple ...
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bioRxiv - Immunology 2024Quote: ... AAS99264.1 and AAO88201.1 respectively) and divided into 3 pools (15-mers overlapping by 10 aa, Sigma-Aldrich, United States or Mimotopes, Australia). For each serotype ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...