Labshake search
Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... Escherichia coli strain BL21 (DE3) cells (Novagen) were transformed using the expression vector and grown at 37°C in a 2× YT broth ...
-
bioRxiv - Microbiology 2023Quote: ... coli F583 (Sigma-Aldrich, St. Louis, MO).
-
bioRxiv - Molecular Biology 2023Quote: ... coli expression vector pET-28c (+) (Novagen, USA).
-
bioRxiv - Biochemistry 2023Quote: ... coli TUNER (DE3) cells (Novagen, Darmstadt, Germany) using the following culture conditions ...
-
bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) codon plus cells (Novagen) in LB rich or M9 minimal media supplemented with 15NH4Cl or 15NH4Cl and 13C-glucose ...
-
bioRxiv - Neuroscience 2023Quote: Lipopolysaccharides from Escherichia coli (BioXtra, Sigma Aldrich) were added to cells at 150 ng/ml for 30 minutes or 24 hours ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli strain Rosetta2(DE3)pLysS (EMD Millipore) was transformed with the pET-based Smt3-Cereblon-TBD expression plasmid ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Coli tRNA were purchased from Sigma-Aldrich. All buffers used are listed in Table S2 and S3 ...
-
bioRxiv - Biophysics 2022Quote: ... coli C43 (DE3) cells (EMD Millipore, Novagen), cells were collected by centrifugation ...
-
bioRxiv - Biophysics 2022Quote: ... coli C43 (DE3) cells (EMD Millipore, Novagen), cells were collected by centrifugation ...
-
bioRxiv - Microbiology 2023Quote: ... coli Rosetta DE3 cells (Novagen, Sigma-Aldrich), and the pQE-80L+pullulanase domain plasmid (henceforth referred to as pQE80L-PULL ...
-
bioRxiv - Microbiology 2023Quote: ... coli Rosetta DE3 cells (Novagen, Sigma-Aldrich), and the pQE-80L+pullulanase domain plasmid (henceforth referred to as pQE80L-PULL ...
-
bioRxiv - Microbiology 2023Quote: ... coli strain B-Type VIII (Sigma-Aldrich), was added at 0.25 μg/ml ...
-
bioRxiv - Immunology 2023Quote: ... coli serotype 0111:B4 (Sigma-Aldrich/Merck) was used as a control of macrophage stimulation ...
-
bioRxiv - Biochemistry 2023Quote: ... coli Rosetta cells (Millipore Sigma, catalogue #70954) were transformed with pET28b-CIITA 1-210 and a single colony was used to inoculate 5 mL LB medium with 50
-
bioRxiv - Biochemistry 2023Quote: ... coli Rosetta2(DE3)pLysS cells (EMD Millipore). Bacteria were grown at 37°C in baffle flasks to an OD600 of ∼0.6 ...
-
bioRxiv - Neuroscience 2024Quote: ... coli BL21(DE3) cells (Novagen, Madison, WI) under 0.5 mM IPTG induction for 4 hr ...
-
bioRxiv - Microbiology 2024Quote: ... coli strains were maintained in LB (Sigma) with antibiotic as needed ...
-
bioRxiv - Cell Biology 2024Quote: ... coli DNA (Sigma D-2001, type VIII), sheared as described (56) ...
-
bioRxiv - Neuroscience 2020Quote: ... All sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 1:1000). Secondary antibodies from Jackson Laboratories were used at 1:400.
-
bioRxiv - Neuroscience 2021Quote: ... The nuclei were stained with DAPI (1:1000, 4’, 6-diamidino-2-phenylindole dihydrochloride; Sigma). The absence of labeling when using sense probes was used to confirm the specificity of the anti-sense probes ...
-
bioRxiv - Neuroscience 2019Quote: ... monoclonal anti-mouse microtubule-associated protein 2 (MAP2; Millipore Sigma, Burlington, MA; 1:1000 dilution), and monoclonal anti-mouse primary conjugated-cy3 glial fibrillary acidic protein (GFAP ...
-
bioRxiv - Neuroscience 2022Quote: ... Nuclei were stained using 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich #D8417, 1:1000).
-
bioRxiv - Biophysics 2023Quote: ... Nucleus were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1:1000; Sigma-Aldrich) for 10 min ...
-
bioRxiv - Neuroscience 2023Quote: ... the fish were anesthetized or euthanized using 1:1000 or 1:500 2-phenoxyethanol (Sigma), respectively.
-
bioRxiv - Cell Biology 2023Quote: ... together with anti-rabbit F(ab′)2 fragment conjugated with CF568 (1:1000, Sigma, SAB4600310).
-
bioRxiv - Molecular Biology 2021Quote: ... with subsequent immunoblotting against either the FLAGepitope tag or TWIN-Strep epitope tag (Anti-FLAG M2, Sigma-Aldrich; THETM NWSHPQFEK antibody, GenScript).
-
bioRxiv - Microbiology 2019Quote: Bacterial strains used in this study (Table S2) were grown in Luria Bertoni (LB) (Sigma), brain heart infusion (BHI ...
-
bioRxiv - Genomics 2019Quote: ... Drosophila S2 cells from the DGRC were grown in Shields and Sang M3 (Sigma S3652) media supplemented with bactopeptone (BD Biosciences 211677) ...
-
bioRxiv - Cell Biology 2019Quote: For acute inhibition of Cdk1 in S2 and U2OS cells the Cdk1 inhibitor RO3306 (Sigma) was added in metaphase or at anaphase onset ...
-
bioRxiv - Biochemistry 2020Quote: ... S2 cells were allowed to settle on coverslips treated with Poly-L-Lysine (Sigma-Aldrich). After gentle washing ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Primers were designed using Primer3 (version 4.0.0) and were synthesized by Sigma (Supplementary information, S2). To ensure there was no spurious amplification ...
-
bioRxiv - Biophysics 2022Quote: Insect S2 cells from the Fehon Lab (UChicago) were maintained in Schneider’s insect media (Sigma) with insect media supplement (Sigma ...
-
bioRxiv - Biophysics 2020Quote: ... was generated by transfecting 25 ml of S2 cells grown in Ex-Cell420 medium (Sigma) with 10 % (v/v ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Oligonucleotide primers used in this study (Supplementary Table S2) were purchased from Sigma-Aldrich (USA). Plasmid DNA was isolated with QIAprep Spin Miniprep kit (Qiagen ...
-
bioRxiv - Cancer Biology 2022Quote: ... using gene-specific primers (Table S2) and FastStart Universal SYBR Green master mix (Millipore-Sigma). Reactions were run in duplicate and relative Ct values were normalized and calculated independently using the –ΔΔ Ct method for the expression of the housekeeping genes HPRT1 and RPL13A (all primers are listed in Table S1).
-
bioRxiv - Cancer Biology 2022Quote: ... using gene-specific primers (Table S2) and FastStart Universal SYBR Green master mix (Millipore-Sigma). Reactions were run in duplicate and relative Ct values were normalized and calculated independently using the –ΔΔ Ct method for the expression of the housekeeping genes HPRT1 and RPL13A (all primers are listed in Table S1).
-
bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
-
bioRxiv - Cell Biology 2020Quote: ... and S-tag (EMD Millipore, MAC112, WB: 1:5000). Rat monoclonal antibody ...
-
bioRxiv - Physiology 2020Quote: ... Plasma TAG was measured with a colorimetric assay (Sigma).
-
bioRxiv - Neuroscience 2019Quote: ... anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich), anti-tubulin # T8660 (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2020Quote: ... HRP-conjugated mouse anti-FLAG tag Ab (Sigma-Aldrich) was used to detect Fab/VH binding ...
-
bioRxiv - Cancer Biology 2020Quote: ... Antibodies to Flag-tag (F3165) were purchased from Sigma. Antibodies to pT308-Akt (ab38449 ...
-
bioRxiv - Plant Biology 2021Quote: ... or western blot using HA Tag Antibodies (Sigma-Aldrich).
-
bioRxiv - Biochemistry 2021Quote: ... FLAG-tag (M2) and α-Tubulin (T5168) from Sigma, RFP (600-401-379 ...
-
bioRxiv - Microbiology 2019Quote: ... The GST tag was cleaved with PreScission Protease (Millipore) and proteins were dialyzed overnight in 50 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Plant Biology 2021Quote: ... against a primary antibody (GST-tag Monoclonal antibody, Novagen), which is attached to GST-tagged TF proteins (Figure S4).
-
bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by thrombin protease (Sigma) in cleavage buffer (20 mM Tris-HCl [pH 8.0] ...
-
bioRxiv - Biochemistry 2021Quote: ... GST tag was cleaved by incubating with thrombin (Sigma) overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... The FLAG tag oligonucleotide was synthesized by Sigma-Aldrich as two complementary single-stranded oligonucleotides (Supplementary Table 1).