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Citations for Millipore Sigma :
9501 - 9550 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Membranes were then solubilized overnight at 4°C in presence of 0.5% n-Dodecyl β-D-maltoside (DDM) (Sigma, D4641). Lysates were used for co-immunoprecipitation using Dynabeads M-280 Streptavidin (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Slices were transferred to Millicell-CM membrane inserts (0.4 μm pore size, 30 mm diameter, 2-3 slices per insert; Millipore Corp) in six well tissue culture treated plates containing MEM with 25% horse serum and 6.5 mg/mL of glucose ...
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bioRxiv - Microbiology 2021Quote: ... 5 mM β-mercaptoethanol and 5% glycerol with minimum of three changes in buffer and concentrated using membrane concentrators (10-kDa MWCO; Millipore). The purified PfGet3-6×his was subsequently used for the custom generation of anti-PfGet3 antibodies or for in vitro binding experiments.
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bioRxiv - Developmental Biology 2020Quote: ... 0.75 μl of egg extract was subjected to SDS-PAGE, transferred to Protran nitrocellulose (Protran, Amersham) or Immobilon membranes (Millipore) and upon blocking using TBST /5% milk or TBST/5% BSA (when phospho-antibodies were used ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins from whole cell lysates (20–60 µg) were separated on 8-10-12% polyacrylamide gels and transferred to Immobilon-FL membranes (Millipore). Primary and secondary antibodies were diluted in blocking buffer (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... dissected hippocampi were cut into 400 μm slices with a tissue chopper and placed on a porous membrane (Millicell CM, Millipore). Cultures were maintained at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resulting multilamellar liposomes were extruded 11 times through two stacked polycarbonate membranes of 0.8 μm pore diameter (Whatman-Nuclepore, Sigma-Aldrich) using a custom-built high-pressure extrusion device or a syringe extruder (Avanti Polar Lipids). ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was resuspended in 50 µl LB and deposited on 22 mm filter membranes with 0.45 μm pores (Millipore, Merck) on a pre-dried LB agar plate ...
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bioRxiv - Immunology 2022Quote: ... 10-15 µg of proteins were loaded onto a 96-well plate with an activated and primed polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma). Proteins adsorbed to the membrane were trypsinized by incubation with the protease for 2 hours at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole-cell lysate was loaded and separated on a 7.5% polyacrylamide gel and transferred onto a PVDF membrane (Merck Millipore, IPVH07850). Membranes were blocked with PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was filtrated using Millex® HV membrane filter units with a pore size of 0.45 µm (Merck Millipore). Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μg of each fraction was separated by SDS-PAGE and transferred to 0.45 μm Polyvinylidene fluoride (PVDF) membranes (Immobilon-P, EMD Millipore).
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (lysate or immunoprecipitation samples) were separated by SDS-PAGE and transferred to 0.45 μm Polyvinylidene fluoride (PVDF) membranes (Immobilon-P, EMD Millipore). For affinity purification of endogenous or epitope-tagged binding proteins ...
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bioRxiv - Cancer Biology 2022Quote: ... were loaded onto a 10% or 12.5% SDS-PAGE gels and transferred to a polyvinylidene fluoride membrane (PVDF; Millipore-Sigma).
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bioRxiv - Cell Biology 2022Quote: ... Fraction 5/6 (found at the interface between 22.5% and 9.5% Nycodenz and enriched in ER and autophagosome membranes (Strømhaug et al., 1998)) was diluted 1.25X and loaded on a Percoll (Sigma-Aldrich; P4937)/Nycodenz gradient (110 μL of 22.5% Nycodenz on bottom ...
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bioRxiv - Cell Biology 2022Quote: ... liposomes were extruded with the LipSoFast-Basic extruder (Avestin) 21 times through 2 polycarbonate membranes (Sigma-Aldrich; WHA10417004 or WHA110405). For testing SMA ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were then subjected to SDS-PAGE (10%) and visualized by silver staining (Pierce) or transferred to PVDF membranes (Millipore). The membrane was blocked in TBS/Tween 0.1% (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... The SDS samples were subjected to SDS polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to Immobilon-P membranes (Millipore, IPVH00010). The membranes were blocked with 5% skim milk (Yukijirushi ...
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bioRxiv - Cell Biology 2022Quote: ... Enhanced chemiluminescence (ECL) signal was generated by incubating the membranes with Immobilon Forte Western HRP Substrate (Millipore. Cat. Nº: WBLUF0100). ECL and fluorescent emission were digitalized with the iBright imaging system (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were separated by 8–15% sodium dodecyl sulfate-polyacrylamide gels and transferred to a PVDF membrane (IPVH00010 Millipore Immobilon). After transfer ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by SDS-PAGE in 5-20% gradient gels (Extra PAGE One Precast Gel, Nacalai Tesque) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). After blocking with 5% skim milk (Nacalai Tesque ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... Twenty µl of each sample were loaded on precast SDS-PAGE gels (Thermo) and transferred to activated 0.2 μm PVDF membrane (Millipore Sigma) for Western blotting ...
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bioRxiv - Immunology 2022Quote: ... removed and faux adherent cells from the lower membrane washed into the bottom well before migrated cells were counted using a Guava EasyCyte 8HT Flow cytometer (Merck Millipore). Data was then presented as the total number of migrated cells or relative to vehicle controls (% control).
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bioRxiv - Immunology 2022Quote: ... resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Laemmli running buffer and transferred to PVDF membranes (Merck-Millipore) in a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... Cell lysates (30 μg/ml) was subjected to SDS-PAGE and transferred onto Immobilon polyvinylidene difluoride membranes (Millipore, Billerica, MA). Protein detection was carried out using anti-human LC3-I/II antibody (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2022Quote: ... After UV cross-linking using a Stratalinker 2400 UV Crosslinker (1,000 microjoules × 100 for 50s], the membranes were hybridized in 10 mL hybridization buffer (Sigma-Aldrich) containing 1 μM of biotin-conjugated RNA probes for 4 h at 42°C ...
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bioRxiv - Microbiology 2022Quote: ... ZIKV was concentrated using an Amicon Ultra-15 Centrifugal Filter Unit with an Ultracel-100 membrane (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Microbiology 2022Quote: ... The samples were boiled for 10 min and separated in a 10% SDS-PAGE gel and transferred to 0.45 μm nitrocellulose membrane (Millipore, USA). For detection of S protein ...
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bioRxiv - Molecular Biology 2022Quote: ... 32P-labeled probes that reverse complement to the targeted miRNAs were hybridized with membrane in PerfectHyb(tm) Plus Hybridization Buffer (Sigma) overnight at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 20 μg of protein extract was resolved in 4-20% polyacrylamide SDS–PAGE gel and transferred onto polyvinylidene difluoride (PVDF) membranes (Immunobilon-FL, Millipore). After blocking with BSA in PBST (Phosphate-buffered saline with 0.1% Tween-20) ...
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bioRxiv - Immunology 2022Quote: ... The membrane was then washed three times and incubated for one hour with HRP-conjugated goat anti-rabbit IgG (Sigma) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The resulting mixtures were then filtered through a Durapore PVDF 0.22-μ m membrane using Ultrafree centrifugal filters (Millipore, UFC30GV00), or MultiScreen Solvinert 96 Well Filter Plate (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The remaining 50 µl of each sample was filtered through a Durapore PVDF 0.22-μm membrane using Ultrafree centrifugal filters (Millipore, UFC30GV00), and 5 µl was injected into the LC/MS.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The lysate was centrifuged at 25000 xg for 30 minutes to remove cell debris and the supernatant was filtered through a 0.22μm membrane filter (Millipore Sigma, USA). Imidazole equivalent to 50 mM final concentration was added to the filtrate containing the MBP-Δ2C-(His)6 protein ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated on 10-12% polyacrylamide gels and transferred onto methanol-activated polyvinylidene difluoride (PVDF) Hybond-P membranes (Millipore). Membranes were blocked in Tris-buffered saline with 0.1% Tween-20 (TBS-T ...
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bioRxiv - Neuroscience 2022Quote: Samples were separated in standard SDS-PAGE gels and transferred to Immobilon-P PVDF membrane (Pore size 0.2 μM, EMD Millipore). The membrane was then blocked in 5% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of protein extract was resolved in 4-20% polyacrylamide SDS–PAGE gel and transferred onto polyvinylidene difluoride (PVDF) membranes (Immunobilon-FL, Millipore). After blocking with BSA in PBST (Phosphate-buffered saline with 0.1% Tween-20) ...
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bioRxiv - Molecular Biology 2022Quote: 15-30 μg of protein extract were resolved on an 8% or 12% polyacrylamide-SDS gel and transferred to a polyvinylidene difluoride membrane (PVDF, Millipore). Primary antibodies were used at the recommended dilutions in 5% milk or 3% BSA diluted in TBS-Tween ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were cut and placed in a 24-well plate and permeabilised in 0.2% Triton-X100 (Sigma-Aldrich, United Kingdom) in PBS for 20 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Pellet containing E64-treated schizonts were resuspended in cRPMI medium and filtered through a 2 µm Isopore polycarbonate membrane (Sigma) to purify free merozoites.
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bioRxiv - Microbiology 2022Quote: ... The cell membrane and nuclei of the midgut tissue were labeled for 30 min with FM1-43 (5 μg/ml) (Sigma) and DAPI (0.5 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated at 100°C for 5 minutes and then separated by SDS/PAGE and transferred to PVDF membranes (Millipore). Membranes were then probed with primary antibodies specific for murine caspase-11 (#C1354 ...
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bioRxiv - Microbiology 2022Quote: ... The resulting virus-enriched filtrate was concentrated by tangential flow filtration (TFF) over a 30 kDa filter (Biomax 30 kDa membrane, catalogue #: P3B030D01, Millipore). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: Immunoblots were performed by resolving 10 μg of RAB or RIPA fraction through 10% denaturing SDS-PAGE and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore). Blots were probed by primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein samples were separated by 10% SDS-PAGE gel and then transferred to a 0.22 μm polyvinylidene difluoride (PVDF) membrane (Millipore, USA) in a wet electron transfer device ...
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bioRxiv - Bioengineering 2024Quote: ... or general membrane cell tracker (Cellvue Claret Far Red, PKH26 Red Fluorescent Cell Linker, or PKH67 Green Fluorescent Cell Linker, Sigma) following the manufacture’s protocol to facilitate downstream analysis ...
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bioRxiv - Immunology 2023Quote: ... Immunoblot detection was achieved by exposure with a chemiluminescence imaging system (PXI Syngene, Ozyme) after membrane incubation with ECL (Immobilon Western, Millipore). The bands intensity values were normalized to that of β-actin using ImageJ software.
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then incubated overnight with primary antibodies in 1X TBST containing 5% BSA (Sigma-Aldrich, St Louis, MO, USA). Membranes with multiple probes were stripped using 0.2 M NaOH before probing with the next primary antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010) using the Mini Trans-Blot cell system (BioRad 1703930 ...