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Citations for Millipore Sigma :
9451 - 9500 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Supernatant recovered after 48h from 293T transfected cells was filtered by a 0.45□μm pore membrane and added to MDA-MB-231 plated cells supplemented with 4□μg/mL polybrene (Sigma-Aldrich). After viral infection ...
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bioRxiv - Neuroscience 2020Quote: ... three organoids embedded in Matrigel (at day 21) and three unembedded organoids were manually dissected from transwell membranes and enzymatically digested with Accumax dissociation reagent (Sigma) with manual trituration ...
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bioRxiv - Neuroscience 2022Quote: ... was cut at the level of the somatosensory cortex and plated on Millicell-CM organotypic culture membrane inserts (Merck Millipore). Slices were cultured in a medium containing 50% Optimem-1 ...
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bioRxiv - Neuroscience 2022Quote: ... dissected hippocampi were cut into 400 μm slices with a tissue chopper and placed on a porous membrane (Millicell CM, Millipore). Cultures were incubated at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore, Massachusetts, USA). The membrane was blocked with either 5% BSA (SRL ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged at 15,000 x g for 15 min at 4 °C and the supernatants were separated in SDS–PAGE and transferred onto Immobilon-FL membranes (Millipore). Following blocking with 5% skim milk in PBS containing 0.05% Tween-20 (PBST ...
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bioRxiv - Genetics 2022Quote: ... Prepared cellular extracts were separated by 13 or 15 % SDS-PAGE and subsequently transferred to an Immobilon-PSQ Membrane (Millipore). The membrane was blocked by 1 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts (in micrograms) of lysates were separated on 4-15% SDS-PAGE gel and transferred to Polyvinylidene difluoride (PVDF) membrane (Millipore). Subsequently ...
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bioRxiv - Cancer Biology 2022Quote: ... Horseradish peroxidase conjugated secondary antibodies were added to the membranes (anti-rabbit, Invitrogen; 31460 and anti-mouse EMD Millipore; AP308P). Membranes were developed with ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and cell surface biotin was cleaved off by incubating the cells on ice with 100 mM of membrane-impermeable reducing agent MESMA (2-mercaptoethane sulphonic acid (Sigma) as described (Nitzsche et al. ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking in 5% BSA, membranes were incubated with Anti-FASN rabbit antibody (#3189, CST) antibody or mouse anti-tubulin antibody (Sigma). Protein was next incubated with HRP-conjugated secondary antibodies and was detected using chemiluminescence.
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bioRxiv - Plant Biology 2019Quote: ... and 10 µg (except for Fig S12 (3 µg)) was loaded on SDS-PAGE before transferring to PVDF membrane (Merck Millipore). SlMai1-myc or synSlMai1-myc proteins were detected using anti-Myc antibodies (GenScript ...
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bioRxiv - Bioengineering 2020Quote: ... the cells migrated through the membrane to the lower layer well were counted by H33342 staining (Sigma, St Louise, USA).
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bioRxiv - Molecular Biology 2019Quote: ... Small RNAs were crosslinked to the membrane by chemical crosslink using N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC, Sigma-Aldrich). Membrane was pre-hybridized for 20 min with PerfectHyb(tm ...
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bioRxiv - Microbiology 2019Quote: ... the sporozoites were separated from unhatched oocysts by filtration using 3.0 µm nitrocellulose membranes (Merck Millipore Ltd., County Cork, Ireland). Fluorescence of filtered samples was evaluated ...
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bioRxiv - Genetics 2019Quote: ... The DNA-bound nuclear extracts were resolved using native 3.5% polyacrylamide gel electrophoresis and were transferred to Immobilon-Ny+ charged nylon membrane (Millipore Sigma) at 400 mA for 1 hr on a Trans-blot SD semi-dry transfer cell (Bio-Rad) ...
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bioRxiv - Bioengineering 2019Quote: Cell lysates were resolved by reducing SDS-PAGE and stained with colloidal Coomassie Brilliant Blue (Carl Roth) or transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore) using the TransBlot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM CaCl2) and concentrated ∼10-fold on an Amicon Ultra-4 centrifugal filter unit with a 10-kDa membrane cutoff (Millipore). PCSK9 in conditioned media preparations was quantified by western blot analysis using purified PCSK9 as a standard and stored in single-use aliquots at −80°C.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 3.4 × 105 cells/cm2 were seeded on Multiscreen™ plates with 0.4 μ polycarbonate Isopore™ membranes (Millipore, MA, USA) in DMEM/High glucose (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... gels were transferred onto 0.45 μm PVDF membranes using a semidry transfer apparatus before being subjected to immunoblot analysis using specific primary antibodies and secondary antibodies (Sigma). Protein detection was performed using ECL chemiluminescent reagent (GE Healthcare ...
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bioRxiv - Developmental Biology 2019Quote: ... the membrane was hybridized with a 32P-labelled probe (TTAAGGG)4 and exposed overnight to Carestream BioMax MR film (Sigma).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples from 24 mice of both strains (n = 3 per group) were separated by SDS-PAGE (8-20%) and transferred onto PVDF membranes (Millipore). Afterward ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were electrotransferred during a 30-min interval from the gel onto an Immobilon-P membrane of pore size of 0.45 µm (Merck Millipore, USA) under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 45-μm polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204 ...
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bioRxiv - Microbiology 2020Quote: ... The unilamellar vesicles were prepared by extrusion of the hydrated lipid mixture through a 0.1 μm Whatman Nuclepore Track-Etched polycarbonate membrane (Sigma, USA) assembled in a LiposoFast Basic apparatus (Avestin ...
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bioRxiv - Neuroscience 2021Quote: ... were loaded on 10% acrylamide SDS-PAGE gels for separation by electrophoresis and then transferred onto PVDF membranes using a transblot (Millipore). The membranes were blocked with 5% milk in Tris-Buffered saline solution with Tween (TBST ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins (40 μg) from cell homogenates were separated on a 10% SDS-polyacrylamide gel and electrotransfered to polyvinylidene fluoride membranes (Millipore). Each membrane was washed with Tris-buffered saline Tween-20 ...
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bioRxiv - Molecular Biology 2020Quote: ... The prepared samples were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). The PVDF membrane was blocked in 5% skimmed milk in Tris-buffered saline containing Tween for 1 hour and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... along with the membrane-impermeable nuclear dye Cytotox Green (Essen BioScience Inc. Ann Arbor, MI) and cell-permeable Hoechst stain (Sigma). Cytotoxicity was measured on an ImageXpress Pico (Molecular Devices ...
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bioRxiv - Microbiology 2020Quote: ... Mosquito homogenates were clarified by centrifugation at 20,000×g (4 °C for 30 min) and filtered through a 0.45 μm membrane filter (Millipore, Billerica, USA). Supernatants were collected and stored at −80 °C ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 120 mL of water sample was also collected per time point and filtered through a 0.2 µm PES membrane filter (Millipore, Inc.) for shotgun metagenomic analyses ...
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bioRxiv - Immunology 2020Quote: ... MS2-FP8 VLPs were concentrated from SEC purified fractions by ultra-centrifugal filtration using Amicon® Ultra-15.0mL 100 K membrane (Merck Millipore Ltd. ...
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bioRxiv - Immunology 2021Quote: ... The CTCS was concentrated by centrifugal filtration (Centricon Plus-70, Ultracel-PL Membrane, 100 kDa size exclusion, Millipore Sigma # UFC710008), with ∼120 mLs CTCS concentrated to ∼0.5 mLs ...
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bioRxiv - Neuroscience 2020Quote: ... dissected hippocampi were cut into 350 μm slices with a tissue chopper and placed on a porous membrane (Millicell CM, Millipore). Cultures were maintained at 37°C ...
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bioRxiv - Systems Biology 2019Quote: Equal amounts of protein were resolved on a 12% SDS-PAGE gel and then transferred to a polyvinylidene difluoride Immobilon-FL membrane (PVDF; Millipore). Membranes were blocked for 30 min at room temperature with 5% skim milk in TBST (50 mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were stained with methylene blue and hybridized to 32P radio-labeled DNA probes (DecaLabel, ThermoFischer) in PerfectHyb™ (Sigma) over night at 65 ºC ...
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bioRxiv - Neuroscience 2019Quote: ... Samples fractionated on Bio-Rad Criterion TGX Precast 4-15% gradient gels were transferred to Immobilon-P transfer membranes (Millipore). Membranes were blocked with 5% milk in TTBS before overnight incubation with primary antibody and visualization using HRP-tagged secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Developmental Biology 2019Quote: ... and non-specific binding sites were blocked by immersing the membranes in blocking buffer containing 5% non-fat milk (70166, Sigma) in TBST buffer for 1 h on a shaker at room temperature or overnight at 4°C ...
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bioRxiv - Bioengineering 2019Quote: For Flag-tag detection membranes were incubated for two hours with the monoclonal anti-Flag M2 antibody from mouse (F3165, Sigma) in a 1:5000 dilution (in TBS-T ...
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bioRxiv - Biophysics 2019Quote: ... All buffers were prepared fresh and filtered through a 0.22 μm polyethersulfone membrane Express™ Plus from Millipore (Billerica, MA). Compressed N2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... subjected to sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS–PAGE) and electro transferred to polyvinylidene difluoride (PVDF) membrane (Millipore, Germany). The membranes were then blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Physiology 2021Quote: ... Frozen sections were used to visualize cardiomyocyte cell membranes by staining with FITC-conjugated wheat-germ agglutinin (Sigma-Aldrich, USA), as described before Zhao et al ...
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bioRxiv - Microbiology 2020Quote: Schleicher & Schuell Minifold II Slot Blot System SRC072/0 44-27570 Manifold was used for transferring samples from sucrose gradient fractions to PVDF membranes (Immobilon PSQ, Merk Millipore). Shortly ...
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bioRxiv - Developmental Biology 2021Quote: ... Followed by covering them in halocarbon oil 27 and sandwiched between the membrane and the cover glass slide (cover glass washed and cleaned with pure ethanol, slide #1.5, Sigma BR470045). The data collection was performed using a custom-built two-photon microscope using 970 nm laser excitation for green (EGFP and mNeonGreen tagged protein and MS2 loops respectively ...
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bioRxiv - Biochemistry 2021Quote: ... After blocking the membrane with 2 % non-fat dry milk dissolved in PBS + 0.1 % (v/v) tween-20 (PBST; Sigma-Aldrich), membranes were incubated with primary antibodies for either 2 hours at room temperature or overnight at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated with primary antibodies specific for TSC22D4 (home-made, by PINEDA Antibody Service, Flag-HRP (#A8592, Sigma), HA (#2999 ...
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bioRxiv - Cancer Biology 2021Quote: 0.08 mg of Cy5.5 in 100 μL water was mixed with 0.2 mg of PLL in 1 mM 100 μL NaHCO3 in the dark at room temperature for 30 min (vortexed every 10 min) and filtered through molecular-weight cutoff membrane filters (10 kDa, Millipore). The resulting PLL-Cy5.5 was collected and washed several times with sterilized water until the color of the filtrate was clear ...
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bioRxiv - Cell Biology 2021Quote: Cell lysates were separated by SDS-gel electrophoresis (15% polyacrylamide for Arfs) and transferred to Immobilon-P PDVF membranes (Millipore). Membranes were blocked with TBST (20 mM Tris ...
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bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...