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Citations for Millipore Sigma :
9551 - 9600 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010) using the Mini Trans-Blot cell system (BioRad 1703930 ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was mixed with loading dye and separated on SDS -12% polyacrylamide gel and transferred onto PVDF Membrane (Millipore) before incubation with rabbit anti-OPTN (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... protein was loaded onto a 10% or 8% SDS-PAGE gel and was transferred to Immobilon-FL PVDF membrane (IPFL00005, Millipore). The blot was blocked for one hour and hybridized with primary antibody overnight in 0.5% fish gelatin (G7041 ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then washed with TBS-T 0.1% three times and developed with Immobilon Forte Western HRP Substrate (Millipore, WBLUF0500). All blots were imaged via chemiluminescence on a ChemiDoc Touch (cat#12003153 ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was filtered through a 4mm PVFD membrane 0.45 μM filter (Cat. No. SLHV004SL, Millipore Sigma, St. Louis, MO) to eliminate proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates (30 μg) were loaded onto a 10% SDS-PAGE and transferred to a nitrocellulose membrane (Millipore, Cat#Z746010). The membrane was blocked with 5% (w/v ...
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bioRxiv - Immunology 2024Quote: Net ALI membrane resistance for each sample was determined by measuring the sample resistance with a MillicellERS-2 Voltohmmeter (Merck Millipore) then subtracting the blank sample resistance reading ...
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bioRxiv - Neuroscience 2024Quote: ... and these were immediately transferred into cell culture inserts over membranes of 0.4 µm pore size (PIC50ORG, Millipore, MA, USA), placed in 6-well culture dishes (Falcon ...
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bioRxiv - Microbiology 2023Quote: ... The elution buffer was further exchanged with Thermo Scientific BupH Tris Buffered Saline (TBS) by using Amicon Ultra-15 Centrifugal Filter Units with Ultracel-10 membrane cutoff (Merck Millipore) and protein concentration was measured with a Qubit 2.0 fluorometer.
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bioRxiv - Microbiology 2024Quote: ... Supernatants were concentrated using Centricon Plus-70 centrifugal filter units with a membrane pore size of 100,000 Da (EMD Millipore) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a PVDF membrane 0.2 μm (cat#10600021, Amersham) and blocked in 5% Bovine Serum Albumin (BSA, cat#A9418, Sigma-Aldrich) for 20 min ...
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bioRxiv - Immunology 2023Quote: ... Immunoblot detection was achieved by exposure with a chemiluminescence imaging system (PXI Syngene, Ozyme) after membrane incubation with ECL (Immobilon Western, Millipore). The bands intensity values were normalized to that of β-actin using ImageJ software.
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bioRxiv - Cell Biology 2024Quote: ... were separated by 8 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore) at 120 V for 2 hours using transfer buffer (25 mM Tris-base ...
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bioRxiv - Immunology 2024Quote: ... the samples underwent sodium dodecyl sulfate-polyacrylamide gel electrophoresis for separation and were then transferred to a polyvinylidene fluoride membrane (Millipore). The membrane was blocked using the primary antibody-blocking solution and then incubated overnight at 4 °C with primary antibodies against P16 ...
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bioRxiv - Immunology 2024Quote: ELISpot assays for OVA-specific IgE and IgG1 antibody-secreting cells (ASCs) were performed using Multiscreen white 96-well filter plates with Immobilon-P PVDF membranes (Millipore). Plates were prepared by pre-wetting with 30 µL of 35% ethanol in water for 1 minute ...
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bioRxiv - Microbiology 2024Quote: ... Protein samples were separated in SDS-PAGE and transferred onto the Immobilon-P PVDF membrane (0.45-µm-pore-size, EMD Millipore). PSB15 protein was detected using affinity purified antibody (1:2000 ...
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bioRxiv - Immunology 2024Quote: ... in RPMI 1640 without serum for indicated times and the cell culture medium (supernatant) was collected and filtered using a membrane filter unit (SLGPR33RS, Millipore). Chloroform methanol purification of proteins from the filtered supernatant was performed and dried proteins were eluted with 1× SDS loading sample buffer and boiled at 96□°C for 10□min.
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bioRxiv - Bioengineering 2024Quote: ... or general membrane cell tracker (Cellvue Claret Far Red, PKH26 Red Fluorescent Cell Linker, or PKH67 Green Fluorescent Cell Linker, Sigma) following the manufacture’s protocol to facilitate downstream analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then incubated overnight with primary antibodies in 1X TBST containing 5% BSA (Sigma-Aldrich, St Louis, MO, USA). Membranes with multiple probes were stripped using 0.2 M NaOH before probing with the next primary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
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bioRxiv - Biophysics 2023Quote: ... 21 passes each through a 200nm and 100nm poly-carbonate Nuclepore Track-Etched Membrane (Sigma-Aldrich, St Louis, MO, USA).
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked using 5% nonfat milk and probed with anti-poly-His primary antibody (cat. no. H1029; Sigma-Aldrich) at 1:3,000 dilution or anti- GST primary antibody (cat ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were resolved under denaturing and reducing conditions using 4 to 12% bis-tris gels (NuPAGE) and transferred to a polyvinylidene fluoride membrane (Immobilon-P, Millipore). Membranes were blocked with 5% nonfat dried milk and incubated with CAMKIIα (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... was prepared by filtering the spent media through a membrane filter with a 0.22 μm pore size (Merck Millipore, USA). MColorpHast pH indicator strip (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Direct blue staining was performed as follows: PVDF membranes were incubated for 10 minutes in 0.008% Direct Blue 71 (Sigma-Aldrich) in 40% ethanol and 10% acetic acid ...
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bioRxiv - Neuroscience 2022Quote: ... The retina was dissociated from the eyecup and mounted RGC side-up as a whole retina or cut in three or four pieces on black nitrocellulose membrane (0.8 mm pore size, Millipore, Ireland).
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bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% bovine serum albumin (BSA) (Sigma-Aldrich, MN, USA) in Tris buffered saline (TBS ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting cell-modified graphite electrodes were encased in a dialysis membrane (14 kDa molecular weight pore size, Sigma-Aldrich) that was previously soaked in deionized water for 1 h before insertion in the electrochemical cell ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was washed twice with TBST and stained with Ponceau S stain [0.5% (w/v) Ponceau S (Sigma-Aldrich) in 1% (v/v ...
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bioRxiv - Biophysics 2023Quote: ... Samples were then transferred to a PVDF membrane and analyzed by Western blot using a mouse-anti-myc primary antibody (Sigma) and HRP-coupled goat-anti-mouse secondary antibody (Jackson ImmunoResearch) ...
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bioRxiv - Developmental Biology 2023Quote: ... The membrane was blocked with 5% milk or BSA (PAN-Biotech, P06-1391100) in Tris-Buffer Saline 0.2% Tween (Sigma, P9416) (TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... All primary antibodies were incubated overnight with membranes in TBS blocking buffer supplemented with 0.1% Tween-20 (Sigma-Aldrich # P1379), while secondary antibodies (HRP conjugate ...
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bioRxiv - Immunology 2023Quote: ... The eluted IgGs were buffered in 1 M Tris (pH=8.0) and then underwent buffer exchange to phosphate-buffered saline (PBS) and concentration using Amicon 30 kDa spin membranes (Millipore). The purified IgGs were stored at 4°C until further use.
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bioRxiv - Microbiology 2023Quote: ... The indexed PCR products were again normalized by loading 15 µl indexed PCR products onto Sequal Prep TM Normalization plates and were pooled and concentrated using a Amicon® Ultra 0.5 ml centrifugal filter (30K membrane, Merck Millipore). A NanoDrop™ One Microvolume Spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Neuroscience 2023Quote: ... the primary neuronal cells X-irradiated with 50 Gy were put into co-culture with unirradiated BV2 microglial cells in NEUROBASAL MED SFM medium using a transwell co-culture membrane insert with pore size of 0.4 μm (Millipore, USA) until analysis.
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bioRxiv - Molecular Biology 2023Quote: ... Switzerland) and further processed by tangential flow filtration (30 kDa MWCO, PES membrane; Pellicon XL 50; Millipore, Merck, Darmstadt, Germany), autoclaved ...
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bioRxiv - Physiology 2023Quote: Fractions were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). For all variants in pET28b except CLC ...
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bioRxiv - Physiology 2023Quote: Purified GS proteins were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). Proteins were dialyzed at 25 °C for 4 hours against 25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were loaded in SDS-PAGE and transferred to Immobilon®-PSQ Transfer Membrane (0.2 μM, Merck Millipore Ltd.) in 1x transfer buffer using a Bio-Rad transfer apparatus ...
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bioRxiv - Developmental Biology 2023Quote: ... 2–3 endodermal explants (2PP or 3/4PP endoderm) were combined with 2–3 mesenchymal explants on Nucleopore membrane filters (Millipore) supported by fine meshed metal grids (Goodfellows) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electrophoretic separated on 4-20% SDS-PAGE precast gels (Beyotime) and transferred to a Hybond P polyvinylidene difluoride membrane (Millipore). The membrane was blocked in 5% skim milk for 2 h at room temperature then incubated with primary antibodies (Rabbit monoclonal to ATPB at 1/50000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... The beads were spun down and the supernatant was passed through a C8 membrane (3 M Empore™, Sigma-Aldrich), to remove any residual beads ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled using a 4 ml Amicon spin concentrator with a 100-kDa molecular weight cutoff membrane (Millipore) and used immediately for grid preparation or hydrolysis measurements.
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bioRxiv - Microbiology 2023Quote: ... The resulting broth MSP was then filter-sterilized by using a 0.22-μm membrane filter unit (Millex®-GP, Merck Millipore Ltd ...
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bioRxiv - Molecular Biology 2023Quote: ... were subjected to SDS-PAGE with 8% or 15% Tris-Glycine gels and transferred to Immobilon- P PVDF membranes (Millipore) using a TransBlot SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS/PAGE on a 12% (vol/vol) acrylamide gel and transferred to PVDF Immobilon-P membranes (Millipore). Primary antibodies specific for caspase-4 (4450S ...
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bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS-PAGE on a 12% (vol/vol) acrylamide gel and transferred to polyvinylidene difluoride (PVDF) Immobilon-P membranes (Millipore). Primary antibodies specific for human IL-18 (MLB International PM014) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts (40 μg) were separated via electrophoresis on 10% sodium dodecyl-sulfate-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Millipore). Membranes were blocked in 2% milk-phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore) using the Trans-Blot Turbo Transfer System (BioRad ...