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Citations for Millipore Sigma :
9301 - 9350 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... After loading the mixed solution into 96-well MultiScreen Filter Plates (product number MSHVN4510, 0.45 μm hydrophilic PVDF membrane, Millipore), filtration was performed by centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto 0.45 µm Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Neuroscience 2023Quote: ... slices were transferred onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 µm pores, Millipore) and kept in six-well culture dishes (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ROS production in BMDMs was measured using the membrane-permeable fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFHDA) (Sigma, #D6883). BMDMs were plated in 96-well plates at 1×105 cells/well ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA was purified by the drop dialysis method using the MF-Millipore Membrane Filter (Merck Millipore, Cat. No. #VCWP04700). Length distribution of the genomic DNA was analyzed by pulsed-field gel electrophoresis ...
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bioRxiv - Genomics 2023Quote: ... 501977679) containing 10% methanol and 0.05% sodium dodecyl sulfate (SDS) onto an activated 0.45um PVDF membrane (Millipore sigma IPVH09120) for 2 hours at 15V except for loading control which was transferred in 1X transfer buffer with 20% methanol at 12V for 90 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was sequentially filtered through a tangential flow filtration cassette with 0.45 µm pore size (PVDF membrane; Pellicon XL 50, Millipore) and concentrated on a 1,000 kDa MWCO tangential flow filtration cassette (regenerated cellulose membrane ...
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bioRxiv - Cell Biology 2023Quote: ... 15 μl of each sample was resolved by 9% SDS-PAGE and transferred to a PVDF membrane (Millipore IPFL00010) and blotted using an anti-EYFP antibody (Thermo MA5- 15256 ...
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bioRxiv - Cell Biology 2022Quote: ... Both types of gel were used throughout study and gels were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, #IPVH85R). The membrane was blocked in 5 % milk-TBST (150 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... The beads were washed 3 × 1 mL with membrane resuspension buffer for KRas before the beads were eluted with membrane resuspension buffer supplemented with 0.2 mg/mL 3X FLAG peptide (SAE0194, Sigma). Aliquots of samples were collected at each stage (input to anti-FLAG resin ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were resolved on 10% SDS-PAGE and transferred to PVDF membrane (Immobilon-FL, Millipore Corp., cat. no. IPFL00010) using a ThermoFisher Powerblotter (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were washed three times for 5 min with Tris-buffered saline with 0.05% Tween 20 (Sigma-Aldrich) and incubated with the secondary (Santa Cruz Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... The cell extracts and SN were resolved in 15% SDS-PAGE gels and transferred to PVDF membranes (#ISEQ00010, Millipore) at 100V during 3 h ...
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bioRxiv - Immunology 2023Quote: ... The diluted reaction mixture was then purified by UF/DF using 10K MWCO PES membrane (Millipore Pellicon 2 Mini) against PBS buffer (30X ...
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bioRxiv - Microbiology 2023Quote: ... and equal amounts of cell extracts were separated by SDS-10 % PAGE and transferred onto a nitrocellulose membrane (Sigma). Samples were then analyzed by western blot using the desired antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... The upper layer was removed and filtered with a 0.45 μm pore size Durapore membrane Ultrafree-HV microcentrifuge filtration unit (Millipore). Samples were mixed with an equal volume of 0.3% NaPTA and 2% sarkosyl and incubated at 37 °C for 10 min before centrifugation for 90 min at 16 ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were then washed three times with 1x PBS supplemented with 0.1% Tween 20 (Sigma Aldrich, P1379; PBST) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... USA) The membrane was stripped and re-stained using an anti-β-actin AC-15 antibody (Sigma Aldrich, USA). Western blot bands were quantified relative to the β-actin loading control using Fiji (ImageJ).[101]
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated overnight with primary antibodies diluted in 5% BSA in TBS (Fisher) with 0.1 % Tween20 (Sigma-Aldrich). Membranes were incubated with secondary antibodies (anti-mouse or anti-rabbit ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 2.5 μL (OD600 ∼0.5) was used to seed sterile 13-mm polycarbonate membrane (WHA10417401, Sigma-Aldrich) on TSA plates and incubated at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in TBS+0.1% tween and milk or 5% bovine serum albumin (Sigma-Aldrich, cat# A3912-50G) in TBS+0.1% tween (phospho-protein samples ...
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bioRxiv - Neuroscience 2023Quote: ... The input sample and washed beads sample were subjected to 8% SDS-PAGE gels and transferred to 0.45 μm PVDF membranes (Millipore), the membrane was blocked by 5% Nonfat dry milk (BIO-RAD ...
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bioRxiv - Microbiology 2023Quote: ... Depending on the culture density up to 1 L of sample was filtered through each (n = 5 per sampling day) 0.22 µm Sterivex polyethersulfone membrane filter unit (Millipore) to obtain biomass for DNA extraction and following amplicon sequencing of 16S and 18S small subunit ribosomal RNA (SSU rRNA ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated for 2 h at room temperature with a monoclonal anti-FLAG antibody (Sigma Aldrich, F1804) diluted 1:1,000 in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... and separated on SDS-PAGE gels (10–12.5% polyacrylamide) and electro-transferred to the polyvinylidene difluoride (PVDF) membrane (Millipore). The membranes were then blocked in 5% nonfat dry milk (NFDM ...
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bioRxiv - Microbiology 2023Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre- soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies bound to the cell mono-layers were detected by the addition of 3,3’,5,5’-tetramethylbenzidine liquid substrate system for membranes (Sigma-Aldrich). The cell culture infectious dose 50% (CCID50 ...
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bioRxiv - Cell Biology 2023Quote: ... SDS samples were subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilon-P membranes (Millipore, IPVH00010). The membranes were blocked with 5% skim milk (Megmilk Snow Brand ...
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bioRxiv - Microbiology 2023Quote: ... Membrane attack complex (MAC) binding was assessed by staining with rabbit anti-human C5b-C9 antibody (Catalog #204903, Sigma) at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903 ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were subjected to SDS-PAGE and transferred onto a polyvinylidene difluoride membrane (IPVH00010; Merck Millipore, Burlington, MA, USA). Antibodies against SPNS1 (HPA041995 ...
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bioRxiv - Developmental Biology 2024Quote: ... centrifuged at 4500 x g at 4°C for 15 mins and filtered using 11 μm filter paper (Whatman) and then 0.65 μM PVDF filter membrane (Millipore). Recombinantly expressed protein was purified by affinity chromatography using Strep-Tactin XT 4Flow High-Capacity resin (IBA LifeSciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA (10 µg) was separated by denaturing agarose gel electrophoresis and transferred onto Hybon-N+ membrane (Sigma-Aldrich) and probed with [32P]dCTP-radiolabelled DNA fragments ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred to a nitrocellulose blotting membrane (Amersham 0.45 µm, 10600016) and blocked in 5% milk in PBS/0.1% Tween (Sigma, P1379) followed by primary antibody incubation overnight in a cold room ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were then centrifuged to remove cell debris and filtered through a 0.45 µm PVDF membrane (Millipore Sigma, SE1M003M00) prior to concentration using a 100 kDa Amicon centrifugal filter concentrator (Millipore Sigma ...
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bioRxiv - Microbiology 2024Quote: ... a 1 μl bait inoculum was spotted on an approximately 5 mm x 5 mm sterile 0.22 μm pore size nitrocellulose membrane (Millipore) positioned at the usual bait inoculation location ...
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bioRxiv - Microbiology 2024Quote: ... A 5 mL of the fixed co-culture was filtered on a polycarbonate membrane filter with 0.1 μm pores (VCTP, 25mm, Merck Millipore). Cells trapped on the filter were washed by passing through 500 μL of pure water five times ...
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bioRxiv - Microbiology 2024Quote: ... 150 to 2,000 mL (Table S1.1) of pre-filtered water were filtered onto 0.22-µm membranes (PES, Millipore Express, Germany). Filters were flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Protein lysates were resolved on 4-12 % precast Bis-Tris gels (Thermo) or poured 7% Bis-Tris gels and transferred to 0.2 μm PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk in TBST and then probed overnight with primary antibodies in 5% non-fat milk in TBST at 4°C ...
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bioRxiv - Physiology 2024Quote: ... to detect newly dead cells from the damage to the integrity of the cell membrane and DAPI (Sigma-Aldrich) to label cell nuclei ...
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bioRxiv - Cell Biology 2024Quote: ... To induce mitochondrial membrane depolarization MEF and HeLa cells were treated with 25µM or 10µM CCCP (Sigma-Aldrich #C2759), respectively ...
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bioRxiv - Microbiology 2024Quote: ... which was then vacuum filtered and dialyzed for 72 hours in water with a cellulose dialysis tubing membrane (Sigma). The colloidal chitin was then homogenized using a Bosch SilentMixx Pro blender ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked using 5% skimmed milk (American Bioanalytical) in TBS with 0.1% Tween 20 (TBST; Sigma-Aldrich) and incubated in TBST overnight at 4°C with the following primary antibodies diluted 1:2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatant containing lentivirus was collected 48 and 72 hr post-transfection and filtered through a 0.45-μm PVDF membrane filter (Millipore). To infect cells ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed for 1 min in 4% formaldehyde and stained for AP activity using the Alkaline Phosphatase Detection Kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with a 1:3 molar ratio of heavy chain and light chain expression vectors using the Expi293 Transfection kit (Sigma-Aldrich; L3287) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: The ability of SHP2 to dephosphorylate the phosphopeptides was evaluated through a malachite green phosphatase assay (PTP assay kit 1 Millipore, MA, USA). The BTAM peptide and the following monophosphorylated peptides derived from known SHP2 substrates were used for comparison ...
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bioRxiv - Neuroscience 2021Quote: ... PDMS with the standard 1:10 weight ratio of crosslinker to base (SYLGARD 184 Silicone Elastomer Kit, Dow Chemical) was diluted with hexane (Sigma-Aldrich, 34859) in a volume ratio of 1:9 ...
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bioRxiv - Genomics 2021Quote: ChIP was performed thrice independently for each antibody using 1×106 SK-N-SH cells for each transcription factor using the EZ-Magna ChIP kit (Millipore Sigma, USA), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Templates used were either bacterial DNA extracted with the GenElute™ Bacterial Genomic DNA Kit or phage DNA extracted from phage lysates that had been precipitated as previously described and then extracted using the GenElute™ Bacterial Genomic DNA Kit with an extended proteinase K incubation of 1 hour (Sigma-Aldrich). 50 ng of template was used for each PCR ...