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Citations for Millipore Sigma :
9701 - 9750 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Eluted proteins were buffer exchanged and concentrated with PBS using Amicon tubes (Millipore). The proteins were further purified by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2021Quote: ... All proteins were concentrated by ultrafiltration with Amicon Ultra (10 kDa, Millipore, Germany) and protein purity was confirmed by SDS– PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated to > 150 μM using centrifugal filters (Amicon Ultra; Merck Millipore), aliquoted and snap-frozen and stored at −80 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein transfer was confirmed with Ponceau S stain (0.1% w/v Sigma-Aldrich). Membranes were blocked for 5 minutes using undiluted EveryBlot Blocking Buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... and total protein was detected with Amido Black (Sigma-Aldrich, St. Louis, MO) as previously described 51 ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was transferred onto PVDF paper (Millipore Sigma, Cat # IVPH00010, Billerica, MA, USA) for 90 mins at 0.5 amps with a Power Pac HC power supply (Bio Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Dried protein hydrolysates were re-dissolved in 16µL HPLC grade pyridine (Sigma 270407) prior to derivatization with 20µL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert–Butyldimethylchlorosilane (Sigma 375934 ...
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bioRxiv - Biochemistry 2021Quote: ... Viral supernatants were filtered (0.45 μm low protein binding filter unit (EMD Millipore)) and concentrated 10-20-fold (Amicon Ultra-15 concentrator with a 100,000-dalton molecular mass cutoff) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then resolved by SDS-PAGE and immunoblotted using GST (Sigma Aldrich), 6His (Sigma Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were released from protein backbone by PNGase F incubation (Sigma Aldrich) for 12 h at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: Thermophilic Odinarchaeota proteins were expressed in Rosetta (DE3) pLysS Escherichia coli cells (Novagen). Cultures were grown at 37°C to an OD600 of 0.3 then cooled to 25°C and further grown to an OD600 of 0.6 and induced overnight with 0.33 mM IPTG ...
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bioRxiv - Plant Biology 2021Quote: Total protein was extracted with 2X Laemmli buffer (Sigma-Aldrich, cat no. 33401) containing 5% β-mercaptoethanol by boiling at 95°C for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected by Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, MA, USA) and a LAS-3000 mini chemiluminescence imaging system (Fujifilm ...
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bioRxiv - Plant Biology 2021Quote: ... FLAG-tagged proteins were detected with Monoclonal ANTI-FLAG M2-Peroxidase (Sigma #A8592) at a dilution of 1:5,000 ...
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bioRxiv - Microbiology 2020Quote: ... The collected HemATN protein samples were concentrated using an Amicon ultrafiltration cell (Millipore) and dialyzed into dialysis buffer (50 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration was determined using a Direct Detect® Infrared Spectrometer (Millipore Sigma).
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bioRxiv - Plant Biology 2021Quote: ... Secreted MIK2ECD-6xHIS and BAK1ECD-6xHIS proteins were purified using Ni-NTA (Novagen) and size-exclusion chromatography (Hiload 200 ...
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bioRxiv - Neuroscience 2020Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Immunology 2021Quote: ... Bound protein was eluted in presence of 1M methyl α-D-mannopyranoside (Sigma). The protein was dialyzed against PBS and subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 GL (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Eluted protein was desalted using PD-10 desalting columns (Sigma-Merck, U.S.A/Germany). Concentration of the desalted protein was estimated using Bradford’s method and the protein was stored at -80 °C until needed.
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bioRxiv - Plant Biology 2021Quote: ... The purified proteins were concentrated using an Amicon Ultra-4 10 K (Millipore).
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration was determined using a Direct Detect® Infrared Spectrometer (Millipore Sigma).
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... the protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). A90C α-synuclein was labelled with 1.5-fold molar excess Alexa Fluor 488 C5 maleimide (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was concentrated using Amicon Ultra-15 centrifugal filter units (Millipore, Germany) to around 8 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were reduced with 10 mM Tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich) for 1 h at room temperature followed by alkylation of cysteine residue side chain thiol groups with iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... Protein was expressed as a His6-tag fusion in BL21(DE3) trxB (Novagen) using autoinduction24 ...
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bioRxiv - Biophysics 2021Quote: ... The protein fractions were pooled and concentrated using 3kDa cutoff ultracentrifugal device (Millipore). The protein was analyzed on 12% SDS-PAGE and mass spectrometry ...
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bioRxiv - Biophysics 2020Quote: ... The protein was then concentrated via centrifugal filters (MWCO 10 kDa; Millipore Sigma) and the buffer was exchanged into final buffer by PD-10 desalting column (GE Healthcare) ...
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bioRxiv - Genomics 2021Quote: ... Dialyzed Tn5 protein was concentrated by using ultracel 30-K column (Millipore, UFC903024) and the quantity of Tn5 was measured by Bradford assay and visualized on NuPAGE Novex 4-12% Bis-Tris gel (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% glycerol with 5000x diluted SYPRO® Orange protein gel stain (Sigma-Aldrich) in total reaction volume 25 μl ...
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bioRxiv - Cell Biology 2021Quote: ... Equal total proteins loads were confirmed by Ponceau red (0.1% Ponceau, Sigma-Aldrich), 5% acetic acid (v/v)] staining of the membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge and flash frozen in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... The protein complexes were eluted with wash buffer containing 10 mM desthiobiotin (Sigma) and treated with TEV protease at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... individual plasmids were transformed into BL21 Rosetta (DE3) protein expression cells (EMD Millipore).
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bioRxiv - Biochemistry 2022Quote: ... the protein was first digested at 16°C with an immobilized pepsin (Sigma) cartridge (2 mm x 2 cm ...
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bioRxiv - Cell Biology 2022Quote: ... bead-bounded proteins were reduced with 100 mM Dithiothreitol (DTT) (Sigma-Aldrich, 43815) and alkylated using 100 mM iodoacetamide (Applichem ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Microbiology 2022Quote: Coding sequences for M proteins were cloned into a modified pET28b vector (Novagen) that contained sequences encoding an N-terminal His6-tag followed by a PreScission protease cleavage site (5) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Proteins were then precipitated upon addition of 50% trichloroacetic acid (TCA, Sigma-Aldrich) to a final concentration of 5% ...
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bioRxiv - Neuroscience 2022Quote: Total protein from A1 was extracted using RIPA buffer (#R0278, Sigma Aldrich, USA) and protease inhibitor cocktail kit (#78410 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were incubated with 3ul anti-FLAG antibody (Clone M2, Sigma-Aldrich) and 50ul Protein-G magnetic beads (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and proteins were transferred to a polyvinylidene difluoride (PVDF; Millipore, Bedford, MA, USA). Membranes were blocked with bovine serum albumin (1 % ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were digested by addition of 20 μL of proteinase K (Sigma, P6556) at 20 mg/ml to the samples and incubation for 2 hours at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated using 10kDa Amicon filter (Merck Millipore, Burlington, MA, USA). The protein concentration in each fraction was estimated using the Bradford reagent (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was quenched with a Roche EDTA protein inhibitor tablet (Sigma-Aldrich) at 1X concentration and the solution was then passed over a CaptureSelect™ IgG-CH1 column (ThermoFischer Scientific ...
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bioRxiv - Microbiology 2022Quote: ... GST-tagged proteins were purified under native conditions on gluthatione-agarose resin (Sigma). The recombinant proteins were then washed 3 times with Buffer A (100mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... and MBP-tagged and associated proteins were eluted in 60 mM maltose (Sigma) for 30 minutes on ice.
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bioRxiv - Cancer Biology 2022Quote: ... filtered through a low protein binding filter unit (PVDF, 0.45 um, Sigma-Aldrich), and stored at 4 degrees C for up to 5 days prior to use ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved E protein was concentrated using a 10 kDa MWCO concentrator (Millipore Sigma) to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare ...