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Citations for Millipore Sigma :
9351 - 9400 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were blocked in 1% BSA (in PBS) for 1 hour at room temperature and processed for PLA using the DuoLink in situ Red Starter Kit (Millipore Sigma #DUO92101) per manufacturer’s instructions ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in the Antibody Diluent Reagent from the kit [mouse anti-c-myc (clone 9E10; 1:200, Sigma-Aldrich #11667149001) and rabbit anti-HA (1:200 ...
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bioRxiv - Immunology 2023Quote: ... The first kit used was the Milliplex® SARS-CoV-2 antigen panel 1 IgA assay (Millipore Sigma, Cat No. HC19SERA1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Bioengineering 2023Quote: We used T7 cysteine constrained heptapeptide (CX7C) phage libraries previously made in our lab(16, 18) using T7Select415-1 cloning kit (Novagen, catalog #70015) to select against CF pHBECs to discover mucus and cell-penetrating peptides ...
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bioRxiv - Microbiology 2020Quote: ... Aspartate- and Coenzyme A Assay Kits (all kits obtained from Sigma Aldrich) utilizing the entire ...
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bioRxiv - Microbiology 2023Quote: ... Aspartate- and Coenzyme A Assay Kits (all kits obtained from Sigma Aldrich) utilizing the entire ...
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bioRxiv - Molecular Biology 2024Quote: ... A commercial cell proliferation kit (Millipore Sigma, Cell Proliferation Kit I (MTT)) was used for mesenchymal stem cells and endothelial cells ...
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bioRxiv - Plant Biology 2022Quote: ... detached leaves were incubated for 30 min in lactophenol Trypan Blue solution (water: glycerol: lactic acid: phenol [1:1:1:1] + Trypan Blue solution [1 mg mL-1; Sigma-Aldrich]). Thereafter ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with primary antibodies (Table 3) diluted in Intercept (TBS) blocking buffer with 0.05% Tween-20 (Sigma-Aldrich) overnight at 4°C on an orbital shaker.
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bioRxiv - Microbiology 2020Quote: ... Fifty µg of protein from each sample were resolved in a 12% (w/v) SDS–PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The phosphorylated fractions of the MAP kinase ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated by SDS-PAGE gel electrophoresis, transferred to Polyvinylidene fluoride (PVDF, immobilon) membranes and blocked in 5% skimmed milk (Sigma) in TRIS-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with either Odyssey Blocking Buffer (Licor Cat #927-40000) or 5% skimmed milk in TBST buffer (SIGMA) and incubated for 1h at room temperature or overnight at 4° ...
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bioRxiv - Microbiology 2019Quote: A sample (10 mL) was removed from the Gambierdiscus culture and filtered through 3-μm membrane (Merck Millipore, Darmstadt, Germany). One hundred microliters of the filtrate ...
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bioRxiv - Microbiology 2019Quote: ... balechii culture was collected from each treatment and was filtered using a 0.22-μm nitrocellulose membrane (Merck Millipore, Darmstadt, Germany). The membrane was then transferred into a 2-mL micro-centrifuge tube containing 1 mL DNA lysis buffer (containing 0.1 M EDTA ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were separated by SDS-PAGE using a 10% polyacrylamide gel (Super SepTM Ace, Wako) and transferred to a PVDF membrane (Millipore). After transfer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 µg of protein lysates were loaded onto an SDS-PAGE gel and wet-transferred to 0.45 µM Immobilon-P PVDF membrane (Millipore, IPVH00010) at 200 mA for 90 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The AFM experiments of NG108-15 cell membranes were performed in the Ca2+-free Ringer solution supplemented with 10mM TCEP (Cat# C4706, Sigma). The TCEP can breakdown the disulfide bonds ...
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bioRxiv - Plant Biology 2022Quote: ... The sample was then centrifuged twice at 13000 g for 10 min at 4°C and the supermnatent was passed through Millex® GV 0.22 μm PVDF membrane filter (Merck Millipore). Next ...
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bioRxiv - Neuroscience 2021Quote: ... maintained for at least 1h at 4°C and then transferred onto semiporous membranes inserts (47 mm diameter, 0.45 µm pore size; Millipore FHLP04700) which were placed in six well tissue culture plates containing 1.1 ml medium per well ...
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bioRxiv - Neuroscience 2021Quote: ... The resolved proteins were then transferred to a nitrocellulose membrane at 220mA for 2 hours and the transfer efficiency was verified by Pounceau staining (Sigma). The membranes were then blocked for 1hour in 5% milk prepared in Tris Buffered Saline with 0.1% Tween-20 (TBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were loaded and run on SDS-PAGE gels, transferred to PVDF membranes, and immunoblotted with antiKv2.1 antibody (K89/34, NeuroMab) and anti-α tubulin antibody (Sigma). The blots were detected using chemiluminescence reagent on a Bio-Rad Chemidoc MP and Image J was used for quantification of detected bands.
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were separated by Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) (814%) and transferred onto Polyvinylidene difluoride (PVDF) membranes (Millipore). Afterwards ...
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bioRxiv - Molecular Biology 2019Quote: Samples were resolved on 12% SDS-PAGE gel and were either silver stained or transferred to PVDF membrane (Millipore IPVH00010). The membranes were probed with Lamin Dm0 (DSHB ADL101 ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 min and separated by 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) before being transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were blocked in 5% nonfat milk for 1 h and incubated overnight at 4 °C with the appropriate diluted primary antibodies or anti-flag/GAPDH antibody ...
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bioRxiv - Microbiology 2020Quote: ... Samples were heated for 5 min at 95°C prior to loading on 12% polyacrylamide gels followed by transfer onto NC membrane (Millipore). 3% BSA in phosphate buffer saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples (5μg) were resolved by electrophoresis on 12% SDS–polyacrylamide gels and electrophoretically transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Corporation) for 20 min at 25 V ...
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bioRxiv - Cell Biology 2019Quote: Cells were seeded onto 24-well insert plates with PET) permeable membranes with 1.0 uM pore size (Millipore product # PSRP010). Seeding volume was 300uL cells at density of .4×106 cells/mL (or 120,000 cells/well ...
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bioRxiv - Immunology 2019Quote: ... the protein lysates or immunoprecipitate samples were separated on SDS-PAGE gels by electrophoresis and then transferred onto polyvinylidene fluoride membranes (Millipore). The membranes were first blocked with 5% (wt/vol ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins from worm lysate were separated using SDS-PAGE gel electrophoresis and transferred to a 0.45 μm PVDF membrane (EMD Millipore) as previously described (Ellenbecker and others 2019) ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were separated by SDS-PAGE on 4-20% gradient polyacrylamide gels and transferred to Immobilon-P PVDF membranes (Millipore) according to standard procedures (Taylor ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lysates were size fractionated on 8 or 15% SDS-PAGE gels using Tris-Glycine-SDS Tank buffer (25 mM Tris, 0.192 M Glycine, 0.1% SDS) and transferred to Immobilon-PVDF membrane (Millipore, Billerica MA). Wet transfer was performed using 1X transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... Western blotted PDVF membranes were blocked in 5% (w/v) non-fat milk powder or 4% (w/v) Bovine Serum Albumin (BSA, Sigma) in PBS-T ...
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bioRxiv - Plant Biology 2020Quote: ... The sterilized eggs were collected from the membrane and placed on 25-μm-pore sieves in 0.01M 2-morpholinoethanesulfonic acid buffer (Sigma-Aldrich) under aseptic dark conditions for 3 days ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by loading whole cell lysates onto an 8%-10% SDS-PAGE gel for electrophoresis and then transferred to a PVDF membrane (Immobilon-P, Millipore). Membranes were incubated with primary antibodies in TBST (TBS ...
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bioRxiv - Cancer Biology 2020Quote: ... media of the 293T/T17 cultures was replaced and following 18 hours of incubation media containing viral particles were harvested and filtered through a 0.45 µm Durapore PVDF Membrane (Millipore SE1M003M00). Viral transfections were carried out over 72 hours ID8 parental cells and transduced cells were selected by their resistance to 2 μg/mL puromycin (MP Biomedicals 0219453910) ...
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bioRxiv - Molecular Biology 2019Quote: ... were subjected to denaturing SDS-PAGE (regularly 12% polyacrylamide gels) resolution and then transferred to PVDF membranes (Immobilon-FL, Millipore). After blocking 1h-3h at room temperature with 5% (w/v ...
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bioRxiv - Plant Biology 2019Quote: ... Pelet was dissolved in 2 ml DDW and passed through a 0.2-μm membrane filter (Millex-GV Filter Unit, Merck Millipore, Ireland). The filtrate was used for sucrose ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were heated and separated on sodium dodecyl sulfate (SDS)-PAGE or Tris-Acetate-PAGE gel-electrophoresis and transferred to PVDF membranes (Millipore). After blocking in phosphate-buffered saline (PBS)/Tween-20 containing 5% nonfat milk ...
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bioRxiv - Bioengineering 2019Quote: The ab-and adsorption of small molecules by the membranes was quantified by immerging them in 10µM rhodamine B (Sigma-Aldrich) in PBS for 2h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The porous membranes were coated on both sides with collagen type IV from human placenta (0.5 mg/mL in water; Sigma-Aldrich) at room temperature overnight ...
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bioRxiv - Microbiology 2021Quote: ... was added to the culture (62) and cells were harvested by collecting the culture on a 0.22-micron membrane (Millipore GTTP). The filter membranes with cells were then cut into small pieces and stored at -80 °C until RNA extraction.
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bioRxiv - Neuroscience 2020Quote: ... ADAM10 and BACE1) or a Novex 10-20% Tris-Tricine gel (Aβ, C83 and C99) followed by blotting on nitrocellulose membrane (Millipore) using the following antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... the samples were separated by 5–20% gradient SDS-polyacrylamide gel electrophoresis (Nagaiki precast gels; Oriental Instruments, Ltd.) and transferred to polyvinylidene difluoride membranes (Millipore). After blocking with Odyssey Blocking Buffer-TBS (LICOR Biosciences ...
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bioRxiv - Biochemistry 2020Quote: SDS samples were run on 10 % SDS-PAGE and transferred to 0.45 μm Immobilon-P PVDF Membrane (Millipore Sigma, IPVH00010) and immunoblotted using anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: Fractions were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, Sigma Aldrich). For all variants except CLC ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked in 5% BSA TBS-T (5% BSA in 1x Tris Buffered Saline, 0.1% Tween 20, Sigma Aldrich) for 1 hour and incubated with primary antibodies overnight in 5% BSA TBS-T (MMP1 1:1000 ab137332 ...
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bioRxiv - Biophysics 2020Quote: ... The virus band was extracted and buffer exchanged to the NTE buffer using an Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-100 membrane (Millipore).
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bioRxiv - Cell Biology 2021Quote: ... the Phos-tag gels were incubated 4 × 10 min in 100 mM EDTA before electrophoretic transfer onto Nitrocellulose membrane (Sigma). Proteins were detected by immunoblotting using the following primary antibody concentrations in 5% milk (VWR)/Tris Buffered Saline -Tween20 (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... mitochondrial or synaptosomal fractions were prepared in Laemmli buffer and separated by SDS-PAGE before transfer to a PVDF membrane (Immobilon-P, Millipore). Membranes were blocked in 5 % non-fat milk in TBS-Tween 20 (0.1 % ...
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bioRxiv - Neuroscience 2019Quote: ... 20 μg per lane Figure S3a) were separated in 11% SDS-polyacrylamide gel before electrophoretic transfer onto Immobilon-P membranes (#IPVH00010; Millipore). Membranes were cut horizontally ...