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Citations for Millipore Sigma :
9401 - 9450 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: The membrane was washed in TBST and incubated with a peroxidase-conjugated secondary antibody (goat, anti-mouse IgG, Sigma-Aldrich) diluted 1 ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of 2 ml of each recipient and donor strains were combined (three mating pairs in total) and filtered through sterile 0.22 µm membranes (Merck Millipore, GSWP02500), which were incubated over TSA plates for 8 days at 22°C ...
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bioRxiv - Neuroscience 2020Quote: ... whole cell lysates were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a 0.2μm pore-size nitrocellulose membrane (Millipore-Sigma, Burlington, MA). After transfer ...
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bioRxiv - Microbiology 2021Quote: ... Collected flow-throughs were pooled for replicates of the same sponge species and concentrated with Pellicon XL50 tangential filtering cassette with 100 kDa cutoff membrane (Millipore). The resultant concentrate (15-20 mL ...
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bioRxiv - Microbiology 2021Quote: ... Equal volumes of total lysates were analyzed using SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore). Rabbit anti-BMV 1a antiserum (1:10,000 dilution ...
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bioRxiv - Physiology 2020Quote: ... Protocol 2 (Pilocarpine): Six participants had 2 intradermal microdialysis membranes inserted and perfused with 1.67 mg/ml pilocarpine (Sigma-Aldrich) prior to the same B2R antagonist and agonist approach described in Protocol 1 (Figure 1B) ...
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bioRxiv - Microbiology 2021Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Plant Biology 2019Quote: Proteins were separated on 12% or 15% SDS-PAGE gels run at 80-120 V for 2 hr and electroblotted on PVDF membrane (Millipore) at 85 V for 3 hours at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Samples taken from the reactor for analysis of acetate and ammonium were immediately centrifuged and filtered with a 0.22 µm pore size filter (PVDF membrane, Millipore, Ireland). PHB and biomass dry weight measurements are performed on 15 mL bioreactor samples ...
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bioRxiv - Molecular Biology 2021Quote: The high molecular weight fraction of 0.33 mg dragline silk was dissolved in the ionic liquid and filtered through a 50k MWCO membrane (Amicon Ultra, Millipore), collected in 100 µL of lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... wet sediment (0.5 g) and the washing buffer collected by filtration through a 0.22 μm membrane filter (Millipore, MP, USA) were used for DNA extraction ...
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bioRxiv - Neuroscience 2021Quote: ... The DIG-labeled probes were hybridized to the membrane in Roche DIG Easy Hyb hybridization buffer (Millipore Sigma cat# 11603558001) at 49°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked in the blocking buffer of 5% BSA in TBST (137 mM NaCl, 2.7 mM KCl [Sigma-Aldrich P9541] ...
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bioRxiv - Biophysics 2021Quote: ... dechorinated with bleach and mounted between a semipermeable membrane (Lumox film, Starstedt, Germany) and a coverslip while embedded in Halocarbon 27 oil (Sigma). Excess oil was removed with absorbent paper from the sides to flatten the embryos slightly ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... the nitrocellulose membranes were blocked with 5% non-fat dry milk in phosphate-buffered saline (PBS) 1X/0.1% Tween 20 (Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 coverslips and Transwell membranes containing both bEND3 and HIFko cells were cut and mounted on Mowiol 4-88 (Sigma) and imaged on a Zeiss LMS880 confocal microscope.
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by size by SDS-Polyacrylamide Gel Electrophoresis (SDS–PAGE) on 10% w/v gels and electroblotted onto a Polyvinylidene fluoride or polyvinylidene difluoride (PVDF) membrane (3010040001, Sigma). Luminata Crescendo Western HRP substrate (10776189 ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected and filtered through a 0.45μm membrane via centrifugation (Durapore PVDF centrifugal filters, Millipore, Billerica, MA, USA). The filtrate was kept on ice until analysis ...
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bioRxiv - Biochemistry 2022Quote: ... Membrane translocation in live cells was induced by the addition of 500 nM phorbol 12-myristate 13-acetate (PMA; Sigma) and monitored by imaging in 20–30 s intervals ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded solution was left static over one day at 4 °C and concentrated with a compressed nitrogen-gas (or air) driven ultrafiltration device (350 ml Amicon Stirred Cell, 3 kDa NMWL membrane, Millipore), during which time cysteine in the solution gradually react to form cystine and precipitated out and could usually clog the ultrafiltration membrane at the end phase of ultrafiltration ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then transferred to a prewet immobilon PVDF transfer membrane (Merck Millipore Ltd; #IPFL00010; Pore Size 0.45 μm) and sandwiched between wet filter paper and cassette holder ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with an alkaline phosphatase-conjugated secondary antibody directed against the first antibody (Sigma Aldrich, Taufkirchen, Germany) for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were separated by electrophoresis in NuPAGE MOPS SDS running buffer and transferred in Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris, 39 mM glycine, 20 % methanol) onto Immobilon-P PVDF membrane (Millipore) using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... dialyzed in buffer C and then concentrated in a centrifugal concentrator with a 10-kDa molecular weight cutoff membrane (Merck Millipore).
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bioRxiv - Physiology 2022Quote: ... SDS-PAGE was performed as previously described.[28] Western blotting using fluorescence detection was performed using Immobilon-FL PVDF membrane (Millipore), Chameleon Duo pre-stained protein ladder ...
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Core Microbiota Drive the Prevalence of Extracellular Antibiotic Resistome in the Water CompartmentsbioRxiv - Microbiology 2022Quote: All of the water samples were initially filtered by a vacuum filter with 0.2-μm pore-size polycarbonate membranes (Millipore, USA). The filters were used to extract iDNA by FastDNA™ Spin Kit for Soil (MP Biomedicals ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was transferred onto a polyvinylidene difluoride (PVDF) membrane and blocked with Tris-Buffered Saline supplemented with 0.1% (vol/vol) of Tween 20 (Sigma®) (TBST ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were electrophoretically transferred to a 0.45 μm nitrocellulose membrane (Amersham) and their presence was analysed using HRP-conjugated anti-Flag (Sigma, A8592) and anti-α-tubulin (Abcam ...
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bioRxiv - Physiology 2022Quote: ... were coated with laminin (100 µg/mL from Engelbreth-Holm-Swarm murine sarcoma basement membrane diluted in PBS, Sigma-Aldrich) and stored in a 12-well plate (VWR) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells having invaded the bottom part of the membrane were counted after paraformaldehyde fixation and nuclei staining with DAPI (Sigma), as described (Renault-Mihara et al 2006).
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bioRxiv - Microbiology 2022Quote: ... Proteins were the resolved in a 12% SDS-PAGE gel and transferred on a 0.2 µm nitrocellulose membrane and then immunobloted using mouse FlagM2 antibody (Sigma-Aldrich) diluted at 1/500.
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of sample were separated on 10-12% SDS-PAGE gels prior to transfer onto Immobilon-P PVDF membranes (Millipore). Membranes were blocked in 5% milk and incubated overnight with primary antibodies diluted in 5% BSA ...
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bioRxiv - Biochemistry 2022Quote: ... SN1 was filtered using an Ultrafree-HV microcentrifuge filtration unit (0.45 μm pore size Durapore membrane, Millipore, Prod. No. UFC30HV00). This was accomplished by loading 500 μl aliquots of SN1 and centrifugation at 12,000g for 30 sec using one filtration unit per ml of SN1 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then fractionated by 12% sodium dodecyl sulfate (SDS)/polyacrylamide gel electrophoresis (PAGE) on polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Immobilon-FL, Millipore). Membranes were blocked with Odyssey Blocking Buffer at room temperature (RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amount of proteins (20 μg) were resolved by 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). 5% skim milk powder or 5% bovine serum albumin in TBST (20 mM Tris-base ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were equilibrated at 4 °C and incubated for 30 min in the presence of 2 mg/ml iodoacetamide (Sigma). Membranes were then solubilized in 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Proteins from SDS-polyacrylamide gels were electrophoretically transferred to nitrocellulose membranes (Schleicher and Schuell) and subsequently stained with Ponceau S (Sigma) to check the loading of the lanes ...
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bioRxiv - Neuroscience 2019Quote: ... 10 µg per sample using two independent microglial lysates per genotype were loaded on a bis-tris acrylamide gel (8% or 12%) and subsequently blotted onto either a PVDF or nitrocellulose membrane (Millipore) using the following antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of cell lysate were resolved on 10% to 12% SDS-polyacrylamide gels and subsequently transferred to a polyvinylidene difluoride membrane (Millipore). The membrane was incubated with the following primary antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... separated in a 5-20% (w/v) gradient SDS/PAGE gel (SuperSep Ace; FUJIFILM) and transferred to a PVDF membrane (Millipore). Blots were blocked with Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2019Quote: ... The immunoprecipitates and 5% inputs were resolved on 10% SDS-PAGE gels and transferred to Immobilon-FL PVDF membranes (Millipore). The following antibodies were used to probe the membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was then blocked with 5% non-fat milk in phosphate buffer saline (PBS)-0.1% Tween20 (Sigma-Aldrich, P1379) for 1h at RT and probed with specific primary antibodies (α-p21 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... dissected hippocampi were cut into 400 μm slices with a tissue chopper and placed on a porous membrane (Millicell CM, Millipore). Cultures were maintained at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore) for 1.5 h ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing EfgA were pooled and concentrated in an Amicon centrifugal filter concentrator with a 10 kDa cutoff membrane (Millipore). The concentrated protein was then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed with PBS-Tween 20 0.05% and incubated with peroxidase-conjugated anti-human IgG antibody (Sigma, USA) at a dilution of 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Cold dissection media was used to separate and rinse the slices before placing them onto Millicell-CM membranes (Sigma-Aldrich). Slices were maintained in culture medium consisting of 25 % (vol/vol ...
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bioRxiv - Immunology 2021Quote: ... equal amounts of cell lysates and immunoprecipitants were resolved by 10-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and were then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore). After incubation with primary and secondary antibodies ...