Labshake search
Citations for Millipore Sigma :
9651 - 9700 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... Total extracted proteins (20 μg) were derivatized according to the manufacturer’s instructions (Millipore). 2-mercaptoethanol was used to reduce samples ...
-
bioRxiv - Cell Biology 2019Quote: ... S-protein-agarose beads and S-tag antibody were purchased from EMD Millipore. Endo H ...
-
bioRxiv - Cell Biology 2020Quote: Protein loading quantity was controlled using a monoclonal anti-α-tubulin antibody (Sigma). Secondary antibodies were from Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: Primary antibodies against the following proteins were used: actin (rabbit, Sigma-Aldrich, #A2066), cMyc (mouse ...
-
bioRxiv - Cell Biology 2019Quote: ... 2 μl lambda protein phosphatase (800 U) and buffer components as instructed (Sigma) and the reaction was terminated after 30 min at 30 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... 300µg of proteins were incubated with 30µl of Streptavidin-Agarose beads (Sigma, CS1638) on a rotating wheel at 4°C for 3hr ...
-
bioRxiv - Immunology 2021Quote: ... The deglycosylated protein was desalted and concentrated using Amicon filter (Merck Millipore, USA). The native protein and enzyme treated proteins were separated through SDS-PAGE and the proteins were transferred to the Nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio Rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... the supernatant was mixed with 30 μL of protein A Sepharose (Sigma P3391) and rotated for 2 h at 4°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma) and Protein G Mag Sepharose (GE healthcare) ...
-
bioRxiv - Immunology 2021Quote: ... and filtering through 0.65 µm low protein binding filter plates (Millipore, Burlington, USA).
-
bioRxiv - Microbiology 2021Quote: ... Purified protein was further concentrated using Amicon Ultra Centrifugal Filter Units (Merck Millipore).
-
bioRxiv - Immunology 2021Quote: ... Priming was done with 50μg of protein in complete Freund’s adjuvant (Sigma, India) per mouse ...
-
bioRxiv - Developmental Biology 2020Quote: ... Proteins associated with 3xFLAG-tagged MBD3 were purified using anti-FLAG sepharose (Sigma) and processed for mass spectrometry as described (Smits et al. ...
-
bioRxiv - Developmental Biology 2020Quote: ... 50μL of pre washed Magna ChIP protein A+G magnetic beads (Millipore Sigma) were then added to the samples and rotated at 4°C for 2 hours to elute the chromatin:antibody complexes ...
-
bioRxiv - Immunology 2022Quote: ... were coated with 20μg/ml OVA protein (Sigma Aldrich, St Louis, MO, USA) or 4μg/ml CII (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with 100 μg/ml of 3×FLAG peptide (Sigma). The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius ...
-
bioRxiv - Molecular Biology 2019Quote: ... and proteins were eluted by boiling with Sample Buffer Laemli 2X (Sigma-Aldrich) for 5 min at 95°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... The captured DNA-RNA hybrids were enriched by protein A-agarose beads (Millipore). The purified DNA was used to construct a library with the NEXTflex Rapid DNA-seq Kit (PerkinElmer).
-
bioRxiv - Molecular Biology 2020Quote: ... The eluates were incubated with 40 μl of S-protein-agarose beads (Millipore) for 2 hr at 4 °C and then washed three times with NETN buffer ...
-
bioRxiv - Cancer Biology 2020Quote: ... FLAG-tagged fusion proteins were immunoprecipitated using Anti-FLAG M2 magnetic beads (Sigma) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... Fractions containing protein were concentrated in 50-kDa-cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in dry ice and stored at -80 °C ...
-
bioRxiv - Bioengineering 2022Quote: ... Eluted proteins were buffer exchanged and concentrated with PBS using Amicon tubes (Millipore). The proteins were further purified by size exclusion chromatography (SEC ...
-
bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 125 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... Supernatants were precleared with 20 μL of protein A/G magnetic beads (Millipore) at 4°C for 1 hour ...
-
bioRxiv - Microbiology 2022Quote: ... the protein content and purity were evaluated with SDS-PAGE and InstantBlue (Sigma) gel staining (Extended Data Fig ...
-
bioRxiv - Immunology 2021Quote: ... proteins were concentrated on a 0.5 mL 10 kDa filter (UFC501024, Merck-Millipore) by ultracentrifugation and purity was controlled by SDS-PAGE.
-
bioRxiv - Cancer Biology 2019Quote: ... The respective protein bands were visualized using the immunobilion chemiluminescence system (Millipore, MA). The densitometry analysis of immunoblotting images was performed using Quantity One Software (Bio-rad) ...
-
bioRxiv - Cell Biology 2019Quote: ... Samples were precleared by a 30 minute-incubation with Protein G agarose (Sigma), and then incubated for 2 hours at 4°C with Protein G agarose and 5 µg of indicated antibodies ...
-
bioRxiv - Immunology 2019Quote: ... Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore, Watford, UK) visualized on Hyperfilm™ (GE Healthcare ...
-
bioRxiv - Cell Biology 2020Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore cat#WBKLS0500) [7].
-
bioRxiv - Bioengineering 2019Quote: ... Antibodies were purified from medium using protein G gravitrap (Sigma, 28-9852-55) according to manufacturer protocol ...
-
bioRxiv - Microbiology 2019Quote: ... proteins precipitated on the beads were eluted using Laemmli’s buffer containing dithiothreitol (Sigma) boiled 5 min at 95 °C ...
-
bioRxiv - Developmental Biology 2020Quote: ... protein synthesis was inhibited by treating cells with 350 µM cycloheximide (Sigma Aldrich).
-
bioRxiv - Cell Biology 2019Quote: ... and DNA loaded proteins were released by incubation with S7 nuclease (Sigma Aldrich) in complete CSK at room temperature (RT ...
-
bioRxiv - Immunology 2019Quote: ... Streptomyces hyaluronlyticus hyaluronidase (HA’se) and biotinylated HA-binding protein (HABP) were from Millipore, and streptavidin was from Thermo Fisher Scientific or eBioscience ...
-
bioRxiv - Molecular Biology 2019Quote: ... FLAG-CTCF-6xHis protein was induced in Rosseta-Gami B-cells (EMD Millipore) with 0.2 M of IPTG at room temperature and was purified with Ni-NTA resin (Qiagen ...
-
bioRxiv - Biochemistry 2019Quote: ... The protein was then concentrated with a 10K Amicon Ultra-15 concentrator (Millipore) and diluted into Storage Buffer (50 mM Tris pH 7.5 ...
-
bioRxiv - Immunology 2020Quote: CD200 and 41BB FAST proteins were digested overnight with TEV protease (Sigma # T4455) at 4 °C in PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... The protein fractions were isolated and concentrated using 3kDa centrifugal filters (Merck Millipore). LC MS/MS was performed on the concentrated culture CMed and spectral analysis was performed ...
-
bioRxiv - Biochemistry 2019Quote: ... and protein concentration was measured using a Bradford assay (Sigma-Aldrich, Taufkirchen, Germany). Proteins were separated with a 10% SDS-PAGE ...
-
bioRxiv - Plant Biology 2020Quote: ... cells were harvested and lysed using BugBuster Protein Extraction Reagent (Novagen, Nottingham, UK), soluble fractions were used over gravity flow Ni2+-NTA sepharose columns (1 ml ...
-
bioRxiv - Genomics 2019Quote: ... and then protein bands were detected using Horseradish peroxidase-conjugated secondary antibodies (Sigma) and Clarity Western ECL Substrate according to manufacturer’s instructions (BioRad) ...
-
bioRxiv - Cell Biology 2019Quote: ... Total protein bands were reversibly stained with Fast Green FCF (Sigma-Aldrich, F7252) and visualized on ImageQuant LAS 4000 (GE Healthcare Life Sciences ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein was eluted using purification buffer supplemented with 5 mM D-desthiobiotin (Sigma). Following affinity purification ...
-
Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... FLAG and Myc tagged-proteins were detected with mAb-FLAG M2 (Sigma-Aldrich) and mAb-Myc 1-9E10.2 (ATCC) ...
-
bioRxiv - Biochemistry 2021Quote: ... bound protein was eluted with binding buffer containing 2.5 mM d-Desthiobiotin (Millipore) and dialyzed into dialysis buffer (50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was treated with > 10-fold excess of TCEP in DMSO (Sigma) and incubated for an hour in the anaerobic chamber at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... The concentrated protein samples were filtrated through Millex 0.22-μm syringe filters (Millipore) and loaded onto a Superdex 200 HR 10/30 gel-filtration column (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated with a 100 MWCO Amicon centrifugal filter unit (Millipore) and purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare ...