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Citations for Millipore Sigma :
9201 - 9250 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... 5 liters of surface seawater were collected in a sterile container and filtered through a 0.22 µm membrane (Millipore). The membrane was stored at −20 °C until processing.
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bioRxiv - Neuroscience 2019Quote: ... Samples were separated by 15% SDS-PAGE gel and transferred onto the 0.45 μm pore size Immobilone-FL Membrane (Millipore) for 1 h at 20 V ...
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bioRxiv - Immunology 2020Quote: ... denatured cell lysates were separated by 10% SDS polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane (Millipore) for probing with specific primary antibodies and HRP-conjugated secondary antibodies ...
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bioRxiv - Developmental Biology 2019Quote: ... then the membranes were incubated overnight at 4 °C under agitation with anti-histone H3 antibody (Sigma, cat. # H0164) diluted 1:10,000 in TBS-T + 1% BSA solution ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions 7-9 were individually collected (500 μL/fraction) and concentrated by Amicon Ultra-0.5 centrifugal filter unit with 10 kDa membrane (Merck Millipore) with 14,000 x g at 4 °C for 8 min centrifugation to have final volume less than 100 μL ...
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bioRxiv - Microbiology 2020Quote: ... 24-hr old biofilms were formed on sterilized polycarbonate membrane filters with a 0.2 µM pore size (Millipore Sigma) by diluting an overnight culture to an OD600 of 0.001 and pipetting 100 µl on 4 membranes on a tryptic soy agar (TSA ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation at 40,000 x g for 30 min at 4°C and the supernatant filtered through a syringe-driven 0.45-μm PVDF membrane (Millipore). To prepare the beads ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were electrophoresed on denaturing SDS-polyacrylamide gels and transferred onto Immobilon-P PVDF membranes (Merck Millipore, USA). The membranes were then blocked using 5% non-fat milk in PBS for 30 minutes followed by overnight incubation at 4°C with primary antibody against protein of interest at the desired dilution in 2% non-fat milk prepared in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Proportional volumes of bacterial and yeast cells were mixed and filtered through 0.45 µm mixed cellulose membrane (Merck, Millipore) before transferring onto IM agar supplemented with 200 µM acetosyringone ...
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bioRxiv - Immunology 2020Quote: ... was then used to block empty sites on the AuNP and filtered via a 0.22 μm PVDF membrane (Millipore). Coated particles were subsequently added to the test antibody solution and incubated for 2 hours at RT before measuring absorbance from 510 to 570 nm on a plate reader ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were blocked with 5% nonfat milk in phosphate-buffered saline with 0.1% Tween 20 (Sigma-Aldrich, P9416; PBST). The blots were incubated overnight at 4°C with primary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... US28-expressing membrane extracts were coated in a 96 well MicroWell™ MaxiSorp™ flat bottom plate (Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The culture medium containing the secreted proteins was harvested by centrifugation (5000 g for 20 min at RT) and filtered through a 0.22 µm membrane filter (Millipore). Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore) in buffer comprising 20 mM sodium phosphate (pH 6 ...
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bioRxiv - Immunology 2021Quote: ... and two pools of S2 peptides (pools 3 and 4) (final concentration of 2μg/mL) on IPVH-membrane plates (Millipore) coated with 5μg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Immunology 2020Quote: ... donkey serum TBS IHC wash buffer and filtered using a centrifugal filter with a 0.1 mm PVDF membrane (Ultrafree-MC, Merck Millipore). Sections were stained with the antibody mix ...
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bioRxiv - Immunology 2019Quote: ... The membranes were blocked with blocking solution [Tris-buffered saline (TBS) (pH 7.4) containing 0.1% Tween 20 (Sigma-Aldrich) and 5% BSA] for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Culture medium containing the secreted fSmChE proteins was harvested by centrifugation (5000 g for 20 min at RT) and filtered through a 0.22 μm membrane filter (Millipore). Recombinant proteins were purified by IMAC using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... ∼500-ml cell suspension containing the secreted IgG was clarified by centrifugation and subsequent filtration through a 0.45 µm HAWP membrane (Merck Millipore). The filtrate was mixed with an equal volume of binding buffer (20 mM Na2HPO4/NaH2PO4 ...
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bioRxiv - Molecular Biology 2019Quote: ... For gel assay reaction mixtures were loaded onto 12% SDS PAGE and then transferred onto PVDF membrane (Merck Millipore) and immunoblotted with antibodies as mentioned ...
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bioRxiv - Microbiology 2019Quote: ... three aliquots of 500 mL of well-mixed seawater taken from the field were filtered through a 0.22 μm mixed cellulose esters membrane (Merck Millipore) using sterile techniques ...
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bioRxiv - Physiology 2020Quote: ... Membranes were then washed using a commercial HRP Substrate solution (Immobilion Forte Western HRP, #WBLUF0100, Millipore Sigma, Oakville, Canada). After protein quantification ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 5.8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol (method described in Table 2) ...
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bioRxiv - Microbiology 2022Quote: ... and equal amounts of cell extracts were separated by SDS-12 % PAGE and transferred onto a nitrocellulose membrane (Sigma). Samples were then analyzed by western blot using the desired antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... the membrane was washed four times with TTBS and secondary antibody goat-anti rabbit-HRP (catalog no. A7058, Sigma) was added at 1:60,000 in 5% milk for 2 hours at RT ...
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bioRxiv - Immunology 2020Quote: ... splenocytes were stimulated with S peptide pools at a final concentration of 2μg/ml on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (clone AN18 ...
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bioRxiv - Neuroscience 2021Quote: ... The SC explants were quickly cut on a stereomicroscope and transferred to culture membranes (Millipore Millicell-CM, Bedford, MA) and cultured in a phenol-red-free Dulbecco’s modified eagle medium (DMEM ...
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bioRxiv - Biophysics 2021Quote: ... The mobile phase buffer consisted of 150 mM AMAC in water and filtered using a 0.22 µm disposable membrane cartridge (Millipore) before use ...
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bioRxiv - Immunology 2020Quote: ... concentrated and desalted 5X using a 10 kDa Amicon® 8400 Ultra filter membrane (Sigma Aldrich, St. Louis, MO). The purified and dialysed native CP extracellular protein was stored at −20 °C until further use.
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bioRxiv - Immunology 2020Quote: ... concentrated and desalted 5X using a 10 kDa Amicon® 8400 Ultra filter membrane (Sigma Aldrich, St. Louis, MO). The supernatant was collected as the cell wall protein fraction and stored −20 °C until further use.
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bioRxiv - Biophysics 2020Quote: Immunoprecipitation samples were fractionated on 6% or 15% SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF) (Millipore). Membranes were blocked in TBS solution (0.05% Tween-20 in PBS containing 5% milk) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were separated on a 12.5% SDS-PAGE gel and transferred to a polyvinylidenedifluoride (PVDF) membrane (Immobilon-P, Millipore). Blots were blocked with 3% non-fat milk-0.05% Tween-20 in Tris buffer saline (TTBS ...
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bioRxiv - Microbiology 2021Quote: ... Lentiviral supernatants were harvested at 72 h post-transfection and filtered through a 0.22 μM membrane (Millipore, Burlington, MA). One day before transduction ...
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bioRxiv - Microbiology 2021Quote: ... The viscous materials including cells after the second step of culture were collected by filtration using a 1.0-μm pore PTFE membrane (Millipore). The trapped materials were gently and carefully recovered from the membrane using MilliQ water with the aid of flat-tip tweezers ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were then subjected to wet transfer (30% MeOH transfer buffer) of histones to 0.22 μm PVDF membranes (Millipore) and protein was detected by Western blot analysis using primary αRb H3K27me3 antibody (Millipore #07-449) ...
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bioRxiv - Microbiology 2020Quote: ... 70 L of groundwater were filtered onto a 0.1 μm pore-size PTFE membrane filter (Merck Millipore, Darmstadt, Germany). The flow-through was collected in a sterilized container ...
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bioRxiv - Immunology 2021Quote: ... peptide cocktails were combined with membrane disrupting antimicrobials effective against relevant pathogens beginning at: 10 μM Cecropin A (Sigma), 500μg/mL ampicillin ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting total membrane fraction was homogenized in TBS buffer containing 1.5% Lauryl-dimethylamine oxide (LDAO) (Sigma, UK). Membrane proteins were extracted by stirring (60 minutes ...
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bioRxiv - Immunology 2020Quote: ... Semidry transfer was performed at 20 volts for 45 minutes onto a polyvinylidene difluoride (Immobilon-FL) membrane (EMD Millipore). Li-Cor Odyssey® blocking buffer (Li-Cor ...
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bioRxiv - Immunology 2020Quote: The assay for membrane depolarization activity of rBjRPS15 and BjRPS1545-67 was performed with the membrane potential-sensitive dye 3,3’-dipropylthiacarbocyanine iodide (DiSC3-5; Sigma) and the bacteria hydrophila ...
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bioRxiv - Immunology 2021Quote: ... The supernatant containing pseudo-virus was centrifuged at 3000g and filtered through a 0.45μm sterilized membrane (Millipore, Burlington, MA). The titer of virus generated by engineered Zhou-COVID-19-Spike plasmid is ten-fold higher than non-engineered Spike protein sequence (data not shown) ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated by electrophoresis on 12.5% SDS-polyacrylamide gels and then transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon; Millipore) using a wet transfer tank (Hoefer Instruments) ...
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bioRxiv - Cell Biology 2021Quote: ... samples were cooled to room temperature and ∼50 µg of proteins were resolved on a 10% SDS-PAGE gel and transferred using a wet transfer setup onto a PVDF membrane with 0.45 µm pore size (Immobilon, Millipore). Membranes were blocked using Tris-buffered saline containing 5% nonfat dry milk and 0.1% Tween 20 (TBST) ...
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bioRxiv - Neuroscience 2021Quote: ... IP samples were resolved on 8% or 10% SDS-PAGE gels and transferred onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were run on SDS-polyacrylamide gels (SDS-PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore). We blocked protein-containing membranes with Odyssey blocking buffer (LiCor ...
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bioRxiv - Neuroscience 2020Quote: ... and the retina was mounted on a nitrocellulose membrane (type AAWB, 0.8 micron pore size, Millipore, Burlington, MA, USA) and fixed by immersion in 4% paraformaldehyde for 30 mins ...
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bioRxiv - Microbiology 2022Quote: ... washed twice with ice-cold buffer A (50 mM Tris-HCl, 200 mM KCl, pH 8.0 at 4 °C, filtered through a 0.22-μM Millipore membrane), resuspended in buffer A with 20 mM imidazole added ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were separated using Sodium dodecyl-sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (IPVH00010, Millipore) at 80V for 3h at 4°C ...
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bioRxiv - Physiology 2022Quote: ... onto PVDF membrane then blotted overnight at 4°C with anti-RBM20 antibody (Myomedix) or anti-γ-tubulin (Sigma) antibodies ...