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Citations for Millipore Sigma :
9151 - 9200 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and membrane blocked overnight while shaking at 4°C in 5% milk (Marvel) containing 0.077% sodium azide (Sigma Aldrich). Primary antibodies were diluted in 5% milk/PBS/0.1% TWEEN 20 at concentrations as follows ...
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bioRxiv - Bioengineering 2020Quote: ... were cut with a 5100mz vibratome (Lafayette Instrument) and cultured on top of PFTE 0.4 µm transwell membranes (Millipore) in 6-well plates ...
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bioRxiv - Plant Biology 2021Quote: ... The solution was then dried using a speed vacuum (Centrivap concentrator, Labconco) and passed through a 0.2-mm membrane filter (Millex-GV filter unit, Merck Millipore). The filtrate was used for sucrose ...
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bioRxiv - Microbiology 2021Quote: ... The mobile phase buffer consisted of 150 mM AMAC in water and filtered using a 0.22 µm disposable membrane cartridge (Millipore) before use ...
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bioRxiv - Neuroscience 2020Quote: ... SCN tissue was isolated from 300 µm slices prepared using a McIlwain Tissue Chopper (RRID:SCR_015798) and cultured on a Millicell filter membrane (Millipore, RRID:SCR_015799) in 1 ml culture medium ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Liposomes were frozen and thawed in liquid nitrogen for 10 rounds and extruded through 0.4 μm polycarbonate membrane (Millipore). 200 μg/ml purified proteins were added into liposomes after incubation with 1% n-octyl-ß-D-glucopyranoside (ß-OG ...
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bioRxiv - Microbiology 2019Quote: ... samples were fixed in 2.5% (v/v) glutaraldehyde in HBSS on IsoporeTM membrane TMTP filters with pore size 5 µm (Merck-Millipore) for 30 min followed by washing thrice with HBSS buffer (10 min each) ...
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bioRxiv - Cancer Biology 2020Quote: ... Intact and well-shaped slices (approximately 5-10 mm diameter) were then placed on top of a porous membrane insert (0.4 µm, Millipore). Then the membrane inserts were placed into 6-well plates containing 1.5 mL maintenance medium consisting of F12/DMEM (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were incubated with 5% skimmed milk in PBS supplemented with 0.05% (v/v) Tween 20 (Sigma, PBS-T) for 30 minutes at room temperature (RT) ...
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miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... The PVDF membrane was sealed with 5% skim milk powder in the PBS containing tween (Sigma-Aldrich; Merck KGaA) for 3 h ...
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bioRxiv - Biophysics 2022Quote: ... Eluted fractions corresponding to the full ternary complex were gathered and spun concentrated to 14.5 mg/mL using an Amicon Ultrafree 10K NMWL Membrane Concentrator (Millipore). Crystals were grown 1-3 µL hanging drops by varying the ratio of protein to mother liquor from 0.5-2:0.5-2 respectively ...
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bioRxiv - Biochemistry 2022Quote: The cell media were made with DNase- and RNase-free water and filtered through 0.22-μm filter membranes (Millex GV, Millipore) before use ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria activity (membrane potential, PE channel) was measured using a tetramethylrhodamine ethyl ester (TMRE) fluorescent probe (Sigma-Aldrich, 87917) at 400 nM for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h.The membrane was incubated overnight in Pierce SuperBlock Blocking Buffer (Pierce #37353) with 0.1% Tween 20 (Sigma #P7949) and validated primary antibodies ...
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bioRxiv - Physiology 2022Quote: ... About 180 handpicked islets were placed onto a polycarbonate membrane (Nuclepore, Whatman) inside a polypropylene perifusion chamber (Swinnex, Millipore) and the oxygenated perifusion solution (modified Krebs-Ringer buffer as above ...
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bioRxiv - Plant Biology 2022Quote: ... The pellets were washed with cytosol extraction buffer for three times and resuspended in membrane extraction buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM DTT, protease inhibitor cocktail (Sigma), 0.5% NP40 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured overnight prior to collecting conditioned media that was filtered through a 0.22-μm pore membrane (Millipore) and treated with 1 mM sodium orthovanadate and Roche Complete Protease Inhibitor Cocktail tablets (according to the manufacturer’s instructions) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by wet transfer (stacking and resolving gel) in transfer supplemented with 20% methanol on 0.45mm PVDF membrane (IMMOBILON-P, Millipore). PVDF was selected over nitrocellulose as it shows an improved transfer of free SUMO (Castoralova et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Wet transfers were carried out onto PVDF membranes, blocked in 5% milk in TBS-T (TBS, 0.1% Tween-20 (Sigma)) and incubated with antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... for 45 s for nuclear staining and blocked in 5% bovine serum albumin (BSA, Biowest) for membrane staining or 5% BSA-0.3% Triton-X (Sigma) for nuclear staining ...
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bioRxiv - Bioengineering 2022Quote: ... Viral supernatant was harvested at 48 and 72 h post-transfection and filtered through a 0.45 μm PVDF membrane (Merck Millipore). For lentiviral infection ...
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bioRxiv - Cell Biology 2022Quote: ... Western blot signal was detected using BioRad ChemiDoc System upon soaking membranes in Luminata Crescendo HRP substrate (Millipore Sigma), ensuring that the signal was not saturated at any pixel.
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bioRxiv - Cancer Biology 2022Quote: ... Then the membranes were washed with TBST and incubated with Immobilon® Western Chemiluminescent HRP Substrate (EMD Millipore Corporation) or ImmunoStar® LD (Wako ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with primary antibodies diluted in 4% bovine serum albumin (BSA) (Sigma-Aldrich, St. Louis, MO, USA) in TBS-T overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg total protein/samples were loaded in each lane of each gel on 7.5-10% SDS-polyacrylamide gel and then transferred to nitrocellulose membrane (Immobilon-P, Millipore). After blocking ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were centrifuged at 12,000 x g for 10 min at 4°C and resultant supernatants were collected and were filtered through a 0.2 mm PTFE (polytetrafluoroethylene) membrane (Millipore). Abscisic acid (ABA)-d6 (gift from Dr ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were centrifuged at 12,000 x g for 10 min at 4°C and resultant supernatants were collected and were filtered through a 0.2 mm PTFE (polytetrafluoroethylene) membrane (Millipore).
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bioRxiv - Microbiology 2020Quote: ... Ten micrograms of the total lysate was separated in 10% SDS-polyacrylamide gels and transferred to PVDF membranes (Millipore). Primary antibodies used in this paper are mouse anti-CMV IE1/2 monoclonal antibody (MAB8131 ...
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bioRxiv - Cell Biology 2021Quote: ... Denaturated and fully-reduced proteins were resolved on Tris-glycine SDS-PAGE followed by western blot analysis onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using enhanced chemiluminescence to detect bound antibodies (Pierce) ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing gp66 were pooled and concentrated (Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane, EMD Millipore) to a final concentration 4 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Growth assays used 200μL culture volumes in 96- well plates (Greneir) sealed using Breathe-Easy® membranes (Sigma Aldrich) and measured attenuance (D ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were incubated in this solution for 30 minutes and then placed on a Millicell-CM membrane (Millipore) in culture medium (50% Minimum Essential Medium ...
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bioRxiv - Cell Biology 2021Quote: Samples were run on 12% SDS-polyacrylamide gels and subsequently transferred to 0.2 μm polyvinylidene difluoride membrane (PVDF) (Millipore). Membranes were washed with 1X TBS and incubated with a 5% solution of BSA for 1 hr at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... lysed in Laemmli SDS-loading dye and separated in 15% acrylamide gels and transferred to 0.45 μm hydrophobic Immobilon-P PVDF membrane (Merck Millipore). Immunoblotting was as described previously 19 ...
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bioRxiv - Microbiology 2020Quote: ... cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18) ...
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bioRxiv - Neuroscience 2021Quote: ... Punches were placed into 100 μm-deep membrane carriers and tissue was surrounded with 20% bovine serum albumin (Sigma) in cacodylate buffer to prevent air pockets ...
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bioRxiv - Microbiology 2020Quote: ... 1 OD600*mL of the donor was added to 1 OD600*mL of the recipient community or isolate and the mixture was plated on a 0.45 μm mixed cellulose ester membrane (Millipore) topping a plate of the recipient’s preferred media without DAP ...
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bioRxiv - Neuroscience 2022Quote: ... Each protein sample was electrophoresed on 10% (w/v) SDS-polyacrylamide gel electrophoresis (PAGE) and subsequently transferred onto 0.22 μm polyvinylidene difluoride (PVDF) membranes (Millipore). Then ...
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bioRxiv - Microbiology 2022Quote: ... The blocked membrane was incubated with the primary antibodies: mouse anti-HA (2,000×, Sigma-Aldrich HA-7, Massachusetts, USA), mouse anti-α-actin (20,000× ...
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bioRxiv - Neuroscience 2022Quote: ... 25 μg of protein were resolved by 8% dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then electrophoretically transferred to a 0.45 μm polyvinylidene difluoride membrane (PVDF; Immobilon, Merck Millipore). Membranes were blocked in blocking buffer (5% non-fat milk or 5% FBS diluted in TBS-T buffer (0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2022Quote: Protein samples were subjected to SDS-PAGE (14% gel) and wet-transferred to a 0.2 μm Immobilon-PSQ PVDF membrane (Millipore) at 110 V for 90 min ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was dried overnight at RT and blocked for 30min with 2%(W/V) BSA in PBS (Sigma), incubated while shaking ON at 4°C with rabbit anti-Histone H3(Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were subjected to SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore Corporation, Billerica, MA). After blocking with 5% milk or 5% BSA in Tris-buffered saline/0.1% Tween for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... detection was carried out by incubating the membrane with the secondary antibody goat anti-rabbit horseradish peroxidase conjugate (Sigma) diluted 1:3000 (or 1:4000 goat anti-mouse (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... Samples were run on a 9% (w/v) SDS-polyacrylamide gel and transferred to an Immobilon-P membrane (Millipore). Membranes were incubated for 1 hour at room temperature in 1xTBST + 5% non-fat milk with primary antibodies (1:2000 α-Myc 9E10 ...
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bioRxiv - Neuroscience 2019Quote: ... All membranes were stripped in Restore Plus buffer (Pierce) and re-probed with rabbit anti-synaptophysin antibody (Sigma-Aldrich) to detect synaptophysin that served as a general marker for presynaptic terminal proteins ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunoprecipitates and 70 μg inputs were analyzed by SDS-PAGE on 7.5% gels and transferred to an Immobilon-FL PVDF membrane (Millipore). Membranes were probed with α-FLAG (Sigma F7425 ...
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bioRxiv - Molecular Biology 2019Quote: ... equal volumes of each fraction were analyzed by SDS-PAGE on a 12.5% gel and transferred to Immobilon-FL PVDF membrane (Millipore). Membranes were probed with α-tubulin (Abcam ab6161 ...
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bioRxiv - Biochemistry 2020Quote: ... A 96-well dot blot apparatus (Schleicher & Schuell) was set up with a 0.45-μm-pore-size polyvinylidene difluoride (PVDF) membrane (Immobilon-P; Millipore), and each dot was rinsed with 500 μl of TBS ...
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bioRxiv - Microbiology 2019Quote: ... The NaCl 0.5 M-eluate (WGA-bound material) was ultradiafiltered against water (OS20LX) in a 0.05-µm VM membrane (Millipore®). The resultant preparations derived from wt-PCN and ov-PCN yeasts were stored at 4 °C ...