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Citations for Millipore Sigma :
9051 - 9100 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: TEM grids functionalized with Strep-AuNPs were blocked with a 3 % (w/v) of bovine serum albumin (BSA) solution (Sigma-Aldrich) diluted in PBS for 15 min to reduce non-specific capture ...
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bioRxiv - Bioengineering 2024Quote: ... Culture supernatants were harvested at 3 days post-transfection and filtered through a 0.45 μm filter (Merck Millipore, Burlington, MA, USA). All proteins were purified with a Talon metal affinity resin (Takara Bio USA ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs were seeded into the collagen gel channel with a media containing 3% dextran (Leuconostoc spp., Mr 450 000–650000, Sigma-Aldrich) at a density of 1 × 107 cells/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed myotubes were blocked and permeabilized in 3% (m/m)-BSA solution with 0.1% (m/m) Digitonin (D5628, Sigma-Aldrich, Germany) in DPBS (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma) in washing solution for 1h ...
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bioRxiv - Cell Biology 2024Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS, 840035), and β-casein from bovine milk (>98% pure, C6905) were purchased from Sigma. BODIPY-TR-C5-ceramide ...
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bioRxiv - Cell Biology 2024Quote: ... GV oocytes were collected from the ovary of 3-4 weeks old C57BL/6 females 48 h after PMSG injection and then were incubated in M2 medium (Sigma, #M7167) supplemented with 10 μM Cilostamide (TargetMol ...
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bioRxiv - Cancer Biology 2024Quote: ... cytoplasmic (cytosolic + microsomal fractions (as described in “cell fractionation” of Methods section) cell extracts were digested with Benzonase (Millipore 70746-3) and precleared with protein-sepharose beads for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cytoplasmic (cytosolic + microsomal fractions (as described in “cell fractionation” of Methods section) cell extracts were digested with Benzonase (Millipore 70746-3) and DNase I (Thermo Fisher Scientific EN0521 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were harvested and enzymatically dissociated into a single-cell suspension in PBS supplemented with 3% fetal bovine serum (#FB-1101, Biosera) and 2 mM EDTA (#ED2SS, Sigma=:Aldrich). After 15 minutes of incubation with 5 nM SYTOX™ Red Dead Cell Stain (#S34859 ...
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bioRxiv - Microbiology 2024Quote: ... and methyl N-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-N-methoxycarbamate (i. e. pyraclostrobin, pesticide analytical standard, CAS 175013-18-0, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Molecular Biology 2024Quote: ... containing a sgRNA sequence (5’-TCATCGCTCACAACCAAGTG-3’) targeting exon 7 of ERBB2 (cloned by Twin Helix) and selected with puromycin (Sigma-Aldrich) for 4 days (1 μg/ml puromycin for 72 hours ...
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bioRxiv - Molecular Biology 2024Quote: In vivo expression of OSKM in all reprogrammable mouse strains was induced by continuous or 3-4 days administration of doxycycline (Sigma, D9891) in drinking water (1 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were dissected free of the eye and incubated with primary antibodies in PBS with 3% Triton X-100 (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
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bioRxiv - Physiology 2024Quote: ... and then transferred for 3 days into new tubes in the presence of food supplemented with 60 µM methoprene (Sigma-Aldrich, St Louis ...
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bioRxiv - Cell Biology 2024Quote: ... They were then IV injected via the common cardinal vein52 with 3 nl of PEVs along with loading buffer (sterile-filtered, endotoxin-free phenol red solution in DPBS; BioReagent, Sigma-Aldrich) giving a nominal dosage range of 105 - 106 particles per bolus estimated by NTA ...
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bioRxiv - Cell Biology 2024Quote: C57/BL6 mice at 9 months of age that were administrated with ABT263 or control vehicles at 3 months of age were injected with 5 IU of eCG (Sigma, G4877). The ovaries were collected at 44 hours after the eCG injection ...
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bioRxiv - Cell Biology 2024Quote: We assessed mitochondrial activity using the 3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (Sigma-Aldrich, Cat# M6494). As a tetrazolium dye ...
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bioRxiv - Immunology 2024Quote: ... 100,000 to 200,000 T-allo10 cells were cultured in a 96-well plate for 6h in total of 200 uL of the base T cell medium (see Primary Cells section) with 3 ug/mL brefeldin A (Sigma Aldrich), 2 uM monensin (BD ...
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bioRxiv - Immunology 2024Quote: ... B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol) FBS and 1% penicillin-streptomycin and activated with 3 μg/mL LPS (Sigma-Aldrich), 10 ng/mL recombinant mouse IL-4 (Tonbo Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... parasite pellets were placed on ice for 5 min before addition of 50 μl chloroform followed by 200 μl methanol:ultrapure water (3:1, including scyllo inositol as an internal standard, 1 nmol, Sigma-Aldrich, I8132). Extraction was facilitated through vigorous vortexing ...
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bioRxiv - Microbiology 2024Quote: For the leukopenic model mice were immunosuppressed with 150 mg/kg of cyclophosphamide (Baxter) on days −3 and −1 plus 1 subcutaneous injection of 250 mg/kg hydrocortisone 21-Acetate (Sigma-Aldrich) on day −1 ...
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bioRxiv - Immunology 2024Quote: The viabilities of the PBMCs or U-937 macrophages were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Microbiology 2023Quote: ... Virus supernatant was removed at 8 hr post-transduction and cells were maintained in fresh growth medium for 48 hr before undergoing 3 µg/mL puromycin (Sigma-Aldrich) selection ...
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bioRxiv - Cell Biology 2023Quote: ... 1.7 μM Acquity UPLC BEH C18 Column with a gradient of solvent A (97:3 H2O:methanol, 10 mM TBA (Sigma Aldrich #90781), 9 mM acetate (Fisher #11350) ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 1% BSA, 1 mM DTT, 0.1 U/µl Sigma Protector RNase Inhibitor) was then added to the cell lysis and the nuclei were pelleted by centrifuging at 500xg for 5 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% TritonX100 in 2 × SSC), anti-bleaching buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 3 mM Trolox (Sigma 238813), 0.8% D-glucose (Nacalai Tesque 168060-25) ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 μM or 1 mM from a 0.5 M stock solution of 3-MB (Sigma-Aldrich, St Louis, MO, USA) solubilized in 0.5 M NaOH ...
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bioRxiv - Plant Biology 2024Quote: ... a similar reaction was performed with both enzymes combined with 10 units phosphoglycerate kinase and 10 units glyceraldehyde-3-phosphate dehydrogenase (both Sigma-Aldrich), alongside 9 mM phospho-enol-pyruvate ...
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bioRxiv - Molecular Biology 2023Quote: ... Freshly resected left ventricular tissue (~3-5 mg per sample) was pulled into fibers and permeabilized in the presence of saponin (50 μg/mL, Sigma, 47036) 30 min at 4°C in BIOPS buffer containing the following ...
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bioRxiv - Systems Biology 2023Quote: ... The first antibody cocktail was prepared in 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich) and filtered through a 0.1 μm centrifugal filter (Millipore ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues were then washed with wash buffer and blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Neuroscience 2023Quote: Experiments were designed to explore the effects of directly applying PCA (Sigma Aldrich, ref. P-5630, CAS number 99-50-3) to the brain (15 mg/kg dissolved in artificial cerebrospinal fluid –ACSF-with 1% dimethyl sulfoxide -DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... for co-immunoprecipitation analysis and into 96-well cell culture plates (3×104 cells/well) coated with 0.01% poly-L-lysine (Sigma-Aldrich, #P9404) for ELISA ...
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bioRxiv - Neuroscience 2023Quote: ... or vehicle (PBS containing cAMP phosphodiesterase inhibitor (100 μM Ro-20-1724; Sigma, #557502 and 500 μM 3-Isobutyl-1-methylxanthine; Sigma, #I5879) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The AAV was concentrated by centrifugation at 2000g for 3-5min at 4°C using an 100k Amicon Ultra Centrifugal Filter Unit (Millipore #UFC810024). After centrifugation ...
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bioRxiv - Bioengineering 2023Quote: ... 100 mM NaCl with 0.1% n-Dodecyl-B-D-Maltoside to remove excess SDS by using an 30 kDa amicon centrifugal filter (1/10 dilution, 3 times; EMD Millipore) before a final overnight dialysis (1/500 dilution ...
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bioRxiv - Microbiology 2022Quote: Seventy-two hour post-fertilised larvae were first anaesthetized for 2 - 3 min in embryo medium E3 containing 0.2 mg/mL Tricaine (Sigma-Aldrich, USA). After which ...
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bioRxiv - Microbiology 2023Quote: Cross-linking experiments were performed by incubation of 2 mM and 5 mM of suberic-bis-acid ester (3-sulfo-N-hydroxyuccinimy (BS3, Sigma-Aldrich) with the purified recombinant protein in 25 mM sodium phosphate pH 7.4 for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Then the slides were washed in running tap water for 3 min before counterstaining in Hematoxylin Solution (Sigma-Aldrich, Cat# 105174) for 10 s.
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bioRxiv - Molecular Biology 2023Quote: ... 500k Day 25 hiPSC-CMs and 100k human Hs27a stromal cells were mixed with 3 U/mL thrombin from bovine plasma (Sigma, T4648) and 5 mg/mL bovine fibrinogen (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... the wells were filled with 200 µl fresh M63 media with or without 3 µM of human transferrin (Sigma-Aldrich #T1147) and incubated at room temperature for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... a catheter was inserted and the infrarenal part was perfused with sterile isotonic saline containing type I porcine pancreatic elastase (2.5-3 U/ml Sigma Aldrich, #E1250) or 0.9% NaCl (sham surgery ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 3 hours and then incubated overnight at 4°C in block with primary antibodies: mouse anti-acetylated tubulin (Sigma, T6793) at 1:250 or rabbit anti-phosphorylated Smad1/5/8 (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... while another portion was concentrated (about 100X) and buffer exchanged to PBS using an Amicon stirred cell with a 3 kDa MWCO cellulose membrane (Sigma-Aldrich). The concentrated product contained T ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed with vDISCO washing solution for 3 hours and 3DISCO clearing60 with 50-70-90-100-100% tetrahydrofuran (THF) (Sigma, 186562), 1h each ...
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Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... Anhydrous 100% ethanol solution was prepared by adding 15 g of molecular sieve beads (Sigma 208582, 3 Å, 8-12 mesh) to 500 mL 100% molecular grade ethanol (Sigma E7023) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... diluted in corn oil (CO, used as vehicle) at 3 mg/kg for 4 days while CO mice received corn oil only (Sigma Aldrich). One cage of n=6 mice per group was used ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.74 mmol of 3 or 4 azidoacids were stirred for 10 minutes at 40°C in minimal dimethylformamide (Sigma-Aldrich, 319937). The solution was then added with 0.74 mmol of DCC (Sigma-Aldrich ...