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Citations for Millipore Sigma :
8451 - 8500 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and 100 μL per well virus solution with or without 1μg/mL TPCK-treated trypsin (Sigma-Aldrich, St. Louis, MO, USA, Cat# 4352157) was added per well ...
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bioRxiv - Genetics 2023Quote: ... then blocking solution was added to each well consisting of PBS containing 0.5% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA). Cellswere incubated in blocking solution for 1 h at room temperature ...
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bioRxiv - Physiology 2024Quote: ... 8.0x104 cells/well were seeded the night prior on a 96-well plate and treated with 5mM 2-deoxy-D-glucose 2h prior to the initial seahorse reading (2-DG; Millipore Sigma cat. D2134). ATP production rates from glycolysis (glycoATP ...
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bioRxiv - Cell Biology 2024Quote: ... cells were dissociated using StemPro Accutase and reseeded in 8 well µ-Slides (IBDI #80826) pre-coated with 15 µg/mL poly-L-ornithine (Sigma #P-3655), 1 µg/mL laminin (Sigma #L4544) ...
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bioRxiv - Immunology 2024Quote: ... the apical surface was washed and transwells were inverted into fresh 24 well culture plates containing HBSS alone or HBSS containing 100 nM N-formyl-methionyl-leucyl-phenylalanine (fMLP; Sigma; uninfected positive control). Neutrophils were applied to the basolateral compartment and transmigration was allowed to proceed for 2h (H292 cells ...
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bioRxiv - Systems Biology 2024Quote: hCMEC/D3 cells were cultured on Lab-Tek™ Permanox™ 8-well chamber slides coated with calf skin collagen (Sigma-Aldrich, UK), prior to immunostaining according to standard protocols74 and using a primary antibody directed against zona occludens-1 (ZO-1 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were plated at a density of 3x105 cells/well in a 24-well plate and treated with either vehicle (sterile phosphate buffered saline [PBS]) or 10ng/mL of LPS (O111:B4 Sigma, L4391, Lot#0000185317) for 18 hours ...
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bioRxiv - Immunology 2020Quote: ... were collected and plated at 5 × 105 cells per well in triplicates per sample in 100 μL of RPMI-1640 (Sigma Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum and 1% Penicillin and Streptomycin ...
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bioRxiv - Neuroscience 2021Quote: ... at a density of 40,000 cells/well in 24-well plates for EdU incorporation assays or on 35-mm tissue culture dishes coated with poly-L-lysine (Sigma, P1524; 30 μg/mL) at a density 10×106 cells/dish for immunoblotting ...
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bioRxiv - Cancer Biology 2019Quote: ... were attached to wells in 12-well (up to two inserts) or 24-well (one insert) plates pre-coated with poly-D-lysine for 15 minutes (Sigma-Aldrich, St. Louis, MO). ∼8×105 cells were seeded to each chamber of the insert in 100 µL resulting in cells at ∼80-90% confluency ...
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bioRxiv - Genomics 2021Quote: ... U2OS cells in 6-well plates were transduced at 50% confluence with 200 μL of 10x concentrated lentivirus and 5 μg/mL polybrene (EMD Millipore TR-1003-G). Approximately 4 days after transduction ...
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bioRxiv - Neuroscience 2019Quote: ... were subcultured in 24-well plates at a density of 105 cells/well (in triplicate) and stimulated with LPS (final concentration of 100 ng/ml; Sigma Aldrich, St. Louis, MO), CpG ODN (final concentration of 2.5 uM ...
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bioRxiv - Cancer Biology 2021Quote: ... MM1.S and U266 myeloma cells were transduced with shRNA-eGFP-containing pLKO.1 lentiviruses in 24-well plates (10 x 104 cells per construct) and in presence of polybrene (Sigma; final concentration 8μg/ml). Medium was changed by centrifugation (5min ...
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bioRxiv - Cell Biology 2021Quote: Freshly sorted mature myoepithelial cells were plated on 16-well chambered coverglasses (Grace Biol-Labs Cat. # 112358) in FAD media supplemented with 10 μm ROCK Inhibitor (Y-27632) (Sigma-Aldrich Cat.# Y0503-1MG) and cultivated for 96 hrs ...
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bioRxiv - Neuroscience 2020Quote: ... as well as α-tubulin as a loading control were analyzed using indicated primary antibodies (α-Tubulin antibody 1:10,000; Sigma-Aldrich, Munich, Germany). After incubation with the appropriate secondary HRP-labeled antibody (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... and 12-well plates containing 18-mm glass coverslips (1 ml/plate) – all plates were coated overnight with poly-D-lysine (Sigma-Aldrich, A-003-E). The cells were cultured in GibcoTM NeurobasalTM Medium (Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... the molds were sterilized using 70 % ethanol and the wells were coated with a Poloxamere solution in ddH2O over night at 4 °C (5 % Pluronic® F-127, Sigma, St. Louis, USA) to render the surface non-adhesive ...
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bioRxiv - Immunology 2022Quote: ... and 50 μM 2-mercaptoethanol (D10 medium) in 12 well plates in the presence of adenosine (0–1 mM) (Sigma, St. Louis, MO, USA); in the presence of each adenosine receptor agonist (0–10 μM ...
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bioRxiv - Microbiology 2021Quote: ... and 10 ng per well in triplicate were used for qPCR using the FastStart™ Universal SYBR® Green Master [Rox] (Sigma-Aldrich, USA) as described [52] ...
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bioRxiv - Immunology 2022Quote: ... the cells were transferred to a single well containing a monolayer of mitomycin C (Cat no. 50-07-7, Millipore Sigma, Merck, Darmstadt, Germany)-treated OP9/ OP9-DL1 cells or were kept unaltered ...
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bioRxiv - Genomics 2022Quote: ... 70% - 80% confluent cells from one well of a 6-well plate were dissociated to obtain single cell suspension and then resuspended in freezing medium (10% DMSO (Sigma Aldrich, Cat. No. D2650), 20% FBS (Millipore ...
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bioRxiv - Biophysics 2022Quote: GUVs were observed either in ibidi 18-well μ-slides (Cat. # 81817) or in chambers created by mounting silicone spacers (Sigma Aldrich, Cat # GBL665106) on # 1.5 coverslips (Superior Marienfeld ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μL of PsVs was added to each well and incubated for 48h-60h in the presence of 10 μg/mL of polybrene (Sigma Aldrich, TR-1003-G). Infection levels were inferred from the amount of luminescence in relative light units (RLUs ...
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bioRxiv - Cell Biology 2020Quote: 8 well Nunc Lab-Tek Chambered Coverglass was coated with 1:100 dilution of fibronectin in DPBS (Millipore Sigma, Burlington, USA, Catalog #FC010-5MG) for 30 mins and then washed with DPBS (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... Pellet was resuspended in DMEM/F12 media and plated at 250,000 cells/mL on 6-well plates that had been pre-coated with 100 mg/ml PDL (Sigma-Aldrich, cat # 27964-99-4) at 37°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... each well of a 6-well plate (Axion Biosystems, catalog no. M384-tMEA-6W-5) was coated with 1 mg/mL Poly-L-lysine (Sigma, catalog no. P2636-100MG). The next day ...
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bioRxiv - Molecular Biology 2023Quote: Cells were grown in EMM or EMM with 0.2 g/L leucine to between 4 x 106 and 1.5 x 107 cells/ml and mounted in Ibidi chambers (8-well, 80827) coated with lectin (Sigma, L1395, at 50 µg/mL). Cells were imaged on a DeltaVision Elite microscope equipped with a PCO edge sCMOS camera ...
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bioRxiv - Cell Biology 2024Quote: Cells were plated in 24-wells with glass coverslips pre-coated with poly-D-lysine (50 μg/mL; MW 70-150 kDa, P6407, Sigma-Aldrich, Canada, Oakville, ON). Cells were treated with the A Disintegrin and Metalloprotease (ADAM)-10 inhibitor ...
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bioRxiv - Biophysics 2022Quote: ... the molds were sterilized using 70 % ethanol and the wells were coated with a Poloxamere solution in ddH2O over night at 4 °C (5 % Pluronic® F-127, Sigma, St. Louis, USA) to render the surface non-adhesive.
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bioRxiv - Microbiology 2023Quote: ... All well plates and culture flasks were coated prior to seeding with 7 µg/mL rat tail tendon collagen I (Sigma-Aldrich, St. Louis, MO) and rinsed with PBS 3 times ...
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bioRxiv - Plant Biology 2023Quote: ... in a petri dish and then placed in 12-well plates containing 90% liquid MS medium (1/4 strength) and 10% polyethylene glycol (PEG, MW 8000, SIGMA, cat # 25322-68-3) (p/v ...
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bioRxiv - Microbiology 2023Quote: ... and Δuge3 (5×104 conidia per well in colorless RPMI) were seeded on laminin-coated (20 µg/mL, Sigma Aldrich, St. Louis, MO, USA) 8-well coverslips and incubated overnight at 33 °C to enable germination ...
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bioRxiv - Molecular Biology 2023Quote: ... Then we added a series of two-fold dilutions of purified human SP-A (0-10 µg/ml, 100µl/well) in TBST containing either 5 mM CaCl2 (Sigma-Aldrich, Saint Louis, MO, USA) or 10 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Cells (5 × 104 cells/cm2) were grown in triplicate in 24-well plates pre-coated with 6 μg fibronectin (Sigma-Aldrich catalog number FC010) and transfected the next day with 500 ng pRL0381 or pRL0385 with TransIT-LT1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The solution in each well was changed every 1-2 days with FSW containing 60□μg/mL penicillin and 50□μg/mL streptomycin (Sigma-Aldrich # P3032 and #S6501).
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bioRxiv - Genomics 2024Quote: ... Pre-differentiated cells were subsequently counted and plated at 400,000-450,000 cells per well of a 12-well plate coated with Poly-L-Ornithine in 1 mL of Maturation medium with 20 uM trimethoprim (TMP) (Sigma-Aldrich, Cat No. 92131) to activate the CRISPRa machinery in these cells (TMP stabilizes the degron-tagged CRISPRa machinery) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mL/well cell-virus suspension was seeded into 50 µg/mL rat tail collagen type I (Sigma Aldrich, St. Louise, MO USA) coated 6-well plates ...
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bioRxiv - Microbiology 2020Quote: ... wells were coated with 100 ng of the respective protein in 100 µl per well coating buffer (Carbonate-bicarbonate buffer, catalogue number C3041, Sigma-Aldrich, St. Louis, MO, USA), then covered and incubated overnight at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... The lungs and metanephric kidneys were dissected in ice-cold PBS and cultured at the liquid-air interface (Grobstein, 1956) using a trans-well membrane culture system supplemented with explant culture medium (DMEM/F12, 10% FBS (Sigma-Aldrich Chemie GmbH; F9665-500ML), 1x GlutaMAX (Life Technologies Europe BV ...
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bioRxiv - Microbiology 2019Quote: ... Coupling validation was performed by incubating 50 µl of each bead suspension (2000 beads/well) with 50 µl α-mouse IgG-Biotin (B7401-1ML, goat anti-Mouse IgG-Biotin, Sigma Aldrich, St. Louis, MO) at 1:1000 dilution in a 96-well flat bottom plate for 2 hours in gentle agitation ...
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bioRxiv - Genomics 2021Quote: ... Proteins were isolated from RPE/choroid as well as from retinal tissue of laser-treated and control mice using RIPA buffer (R0278, Sigma-Aldrich, St. Louis, Missouri, USA) containing protease (Complete Tablets Mini ...
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bioRxiv - Bioengineering 2021Quote: ... GCaMP6f-expressing HEK cells were seeded on a twelve-well cell culture plates with 18-mm glass coverslips coated with poly-D-lysine (PDL) (10 μg/μl; P6407, Sigma-Aldrich, St. Louis, Missouri, USA) for 1-2h ...
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bioRxiv - Neuroscience 2021Quote: ... 500µL of conditioned media was collected from each 1mL well for AlphaLISA assay and macrophages were loaded with MitoSOX™ Red (Sigma Aldrich, St. Louis, Mo) mitochondrial superoxide indicator at a concentration of 1µM in complete ...
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bioRxiv - Molecular Biology 2020Quote: ... The media was then carefully removed from the 10 cm dish and the enriched myoblasts population was seeded in a 6 well-plate coated with Matrigel (Sigma-Aldrich, St. Louis, MO, USA) in plating media ...
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bioRxiv - Microbiology 2021Quote: ... wells were coated with 100 ng of the respective protein in 100 μL per well coating buffer (Carbonate–bicarbonate buffer, catalogue number C3041, Sigma-Aldrich, St. Louis, MO, USA). Following an overnight incubation at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... wells were coated with 100 ng of the respective protein in 100 μL per well coating buffer (Carbonate–bicarbonate buffer; Sigma-Aldrich, St. Louis, MO, USA). Following an overnight incubation at 4°C ...
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bioRxiv - Zoology 2021Quote: ... cardiac troponin I (cTnI) and hyperpolarization activated cyclic nucleotide gated potassium channel 4 (Hcn4) as well as nuclei using DAPI (Sigma-Aldrich, dilution 1:1000, D9542). For SMA we used the primary antibody RRID:AB_476701 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: Disks of 1 cm in diameter were cut from Aclar® foil (thickness 198 μm; TedPella, 10501-10) and placed in sterile Corning ® 12-well plates (Sigma-Aldrich, CLS 3737). Dissociated cells were plated on the these disks (cells from 1 blastoderm /disk ...
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bioRxiv - Molecular Biology 2020Quote: Six-day-old seedlings of SYT1-GFP/1xPHFAPP1 were incubated in wells containing 60 µM of PAO (Sigma, PAO stock solution 60 mM in DMSO) in one half strength liquid MS for 4 hours ...
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bioRxiv - Physiology 2023Quote: ... Isolation of 40-60 renal segments was performed according to localization in the kidney (cortex vs medulla) and well-defined morphologic characteristics under binocular loupes after kidney treatment with Liberase (Sigma-Aldrich, St Quentin Fallavier, France) as described in (25) ...