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Citations for Millipore Sigma :
8151 - 8200 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 2-3 mm3 tumor pieces were cut and placed in a 24-well plate containing RPMI medium supplemented with 10% human AB serum (Sigma-Aldrich), 6000 IU/ml human recombinant IL2 (Peprotech) ...
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bioRxiv - Systems Biology 2020Quote: ... one well containing peripheral blood monocyte- or human pluripotent stem cell-derived macrophages were stimulated with 10ng/ml Lipopolysaccharide (LPS) (Sigma-Aldrich; Salmonella enterica serotype minnesota ...
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Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: HEK293 cells were seeded into a 96-well plate at a density of 4×104 cells/well with DMEM/F-12 medium (Fujifilm Wako Pure Chemical) containing 10% fetal bovine serum (FBS, Sigma-Aldrich) and 0.5% Gibco penicillin-streptomycin mixture (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... and 1 mM Tris-2-carboxyethyl-phosphine) containing ATP-regeneration and oxygen-scavenging systems as well as 1 mg/mL bovine serum albumin (Sigma-Aldrich). The beads were trapped by two 1064-nm lasers in a passive-force clamp mode ...
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bioRxiv - Genomics 2019Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B-27 with penicillin/streptomycin, and 5 mg/mL aminocaproic acid, Sigma-Aldrich). 3D-EHTs were supplied with 2.5 mL/well of fresh 3D-EHT media three times per week.
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bioRxiv - Cell Biology 2019Quote: Confluent HeLa cells grown in 6- or 12-well plates were treated with deferiprone (DFP; 379409, Sigma-Aldrich; working concentration, 1mM) for the time durations as indicated.
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bioRxiv - Systems Biology 2019Quote: ... were plated into a LN511-coated 12 well plate in the standard media supplemented with 8 μg/mL polybrene (Sigma-Aldrich). Each well received a virus amount equal to MOI = 0.3 ...
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bioRxiv - Immunology 2019Quote: ... and seeded (2×105/well at memory time point or 2.5 x105/well at effector time point in respective experiments) in serum-free unbuffered RPMI 1640 medium (Sigma-Aldrich # R6504) onto Cell-Tak (#354240 ...
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bioRxiv - Immunology 2020Quote: ... miR-511-3p transfected cells as well as controls were stimulated on day 6 with RSG in the presence or absence of LPS (Sigma Aldrich) for 24 hours before harvesting ...
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bioRxiv - Cell Biology 2020Quote: ... Thirty micrograms of protein per well were run on 10% SDS-polyacrylamide (sodium dodecyl sulphate polyacrylamide) gel electrophoresis and then transferred to PVDF membranes (Millipore, USA). Blots were blocked in 5% bovine serum albumin at room temperature for 1 hour and incubated with primary antibodies overnight at 4° followed by incubation with corresponding secondary antibodies at room temperature for 1 h ...
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bioRxiv - Biophysics 2020Quote: ... The created wells were washed three times with PBS buffer and then incubated with BSA-biotin (0.5 mg/mL, Sigma-Aldrich, USA) and NeutrAvidin (0.2 mg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were dispersed with Accutase at d45 of differentiation and plated at a concentration of 3×105 cells/well into MEA plates in 50 μL of culture media containing 10 μg/mL laminin (Sigma, 11243217001). One hour after plating ...
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bioRxiv - Neuroscience 2021Quote: ... slices were transferred to a 12-well plate with 1 mL freshly oxygenated ACSF and incubated with either 1mM anisomycin (Sigma #A9789) or vehicle (0.5% DMSO ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in 3 ml StemFlex containing 10 μM Thiazovivin (Millipore, #S1459). The cells were subjected to a 48 hour cold shock at 32 °C to enhance homology directed repair (HDR) ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in StemFlex containing 1 μM H1152 (Millipore, #555550). On the next day ...
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bioRxiv - Developmental Biology 2020Quote: ... electroporated retinas were placed on a 35-mm glass bottom dish (Pelco, Wilco Wells) and incorporated in collagen prepared as follow: 100 mg of rat tail collagen type VII (Sigma-Aldrich) was dissolved in 6ml of 16.7 mM acetic acid overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... was performed in QuantStudio 5 384 Optical well plate system (Applied Biosystem) in a standard 10 ul with the 2X SYBR FAST qPCR Master Mix (Sigma-Aldrich) with gene specific primers (Supplementary Table 1 ...
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bioRxiv - Genetics 2021Quote: ... cells were inoculated into TAP medium lacking nitrogen and 200 μl-aliquots were tranferred daily to a multi-well plate and mixed with Nile Red (Sigma, 72485) to a final concentration of 1 μg ml−1 (Msanne et al. ...
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bioRxiv - Microbiology 2020Quote: ... the biofilm-coated glass beads were gently picked and submerged in 0.9% NaCl solution for 3 times using sterile tweezer to remove the unbound cells before transferring to new 24-well plates that consist of different concentrations of colistin sulfate (Sigma-Aldrich) and auranofin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: Vero E6 cells were seeded in 48-well plates and at the next day preincubated in DMEM (supplemented with 10 % inactivated FBS) with the different inhibitors (Sigma, Germany) for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... or 3 × 104 cells/well for surface staining on 12-mm coverslips in 24-well plates coated with 20 μg/ml poly-(D)-lysine (Sigma-Aldrich). Neurons were maintained in serum-free Neuralbasal™A media (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The plates were incubated for 3 days at 37°C and the cytopathic effect determined by staining with 100 µl/well crystal violet solution (spatula tip (∼4 mg) crystal violet powder (Sigma Aldrich) solved in 30 ml 37% formalin and 120 ml PBS (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B27 with penicillin/streptomycin, and 5 mg/ml aminocaproic acid; Sigma-Aldrich). 3D-EHTs were supplied with 2.5 ml/well of fresh 3D-EHT media three times per week.
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bioRxiv - Molecular Biology 2021Quote: ... half of the wells were cultured in E6 medium supplemented with 200 ng/mL FGF9 (RD systems) and 1 μg/ml heparin (Sigma-Aldrich) until day 7 (anterior intermediate mesoderm (AM)) ...
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bioRxiv - Molecular Biology 2021Quote: FSHD immortalized myoblast cells were seeded in 6-well plates pre-coated with extracellular matrix gel from Engelbreth-Holm-Swarm murine sarcoma (Sigma, UK). Following transfection with PMOs ...
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bioRxiv - Immunology 2020Quote: ... splenic mononuclear cells at 1×106 cells/well were stimulated with 25 ng/ml of phorbolmyristate acetate (PMA) and 1 µg/ml of ionomycin (Sigma-Aldrich) in complete RPMT 1640 medium in the presence of 3 µg/ml of BFA for 5 hours at 37 °C in 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... and Grace Bio-Labs reusable CultureWellTM gaskets (3 mm x 1 mm, well capacity 3-10 ul, Sigma-Aldrich, GBL103250-10EA) were cleaned 3 times sequentially in 100% isopropanol and Milli-Q H2O ...
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bioRxiv - Biochemistry 2021Quote: ... and 500 μL) were added to each well along with a no virus control with 7 μg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight ...
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bioRxiv - Synthetic Biology 2021Quote: ... Carboxyl groups on the photo-activated regions were activated with 50 µL/well of 0.05 g/mL N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (Sigma-Aldrich, 03450) and N-Hydrosuccinimide (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cell medium was removed and replaced with 200 µL/well of 2 µM DiI in DPBS with Mg2+ and Ca2+ (Sigma-Aldrich) to stain the cell membranes ...
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bioRxiv - Cell Biology 2021Quote: ... as well as secondary antibody of 1:5000 dilution in conjunction with Luminata™ Classico Western HRP substrate (Millipore Corporation, WBLUC0500).
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Cas9 viral supernatant was added to 1×106 cells growing in a 6-well plate plate in 3mL of complete media with polybrene (4mg/mL) (Sigma, H9268). The cell-virus mixture was centrifuged at 3000rpm at 25°C for 1h followed by incubation at 37°C overnight ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... a polytetrafluoroethylene mold made by milling was used to cast 36 hemispherical wells on a thin layer of 2% agarose (Cell culture grade, Sigma-Aldrich) in ddH2O in a 35×10mm petri dish ...
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bioRxiv - Developmental Biology 2021Quote: ... 5×104 cells were seeded onto ultra-low adherence 6-well culture plates in 2 mL of serum-free DMEM-F12 media (Sigma-Aldrich), supplemented with 10 µg/mL Putrescine ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded into 96-well plates and transfected with E2F1 luciferase reporter plasmids (50 ng/well) using X-tremeGENE HP DNA Transfection Reagent (#06366236001, Sigma-Aldrich). After 12 hours ...
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bioRxiv - Bioengineering 2021Quote: ... Plates were washed thrice with PBS-T followed by the addition of 20 μL per well of Streptavidin-Peroxidase polymer (Sigma #S2438) diluted in dilution buffer to 1.25 ng/μL ...
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bioRxiv - Neuroscience 2020Quote: ... a single organoid per well was transferred on 6 well plates on day 77 and 120 in Diff2 medium with 20 ng/ml LPS serotype O111:B4 (Sigma # L2630) and incubated for 24 h ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were seeded in 96 well plates at 1.5×106 cells/ml in the presence or absence of 10ng/ml of lipopolysaccharide (LPS; Sigma-Aldrich, USA). The aqueous extracts from the plants were added to the stimulated or unstimulated cells at three different concentrations of 50 ...
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bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) added to the wells ...
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bioRxiv - Immunology 2021Quote: ... GC B cells were added starting from 3000 cells/well to 1 cell/well through 3-fold dilution steps in the presence of 30 μg/ml of LPS (Salmonella typhimurium, Sigma-Aldrich) and 2 ng/ml of IL-21 (Peprotech) ...
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bioRxiv - Biophysics 2020Quote: ... MEFs transfected with LIM-protein plasmids were plated in 4-well Strex chambers (STB-CH-4W, 2 cm2 × 4 chambers) coated with 50 μg/mL fibronectin (Millipore, FC010), and allowed to recover overnight ...
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bioRxiv - Cell Biology 2021Quote: ... medium was removed from the wells and cells were washed once with PBS and fixed in prechilled 10% trichloroacetic acid (TCA) (Sigma-Aldrich) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Brains and spinal cords were dissected and placed in 6 well plate with cold dissection buffer (HBSS no cations with 5ug/ml actinomycin, 10uM triptolide, and 27ug/ml anisomycin (Sigma A9789)) protected from light ...
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bioRxiv - Immunology 2022Quote: ... the primary oligodendrocytes were plated at a density of 100,000 cells per 24 well plate on 10% PLL (Sigma, P4832-50ML) and 1.5% laminin (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Cell number and viability were examined each day in triplicate wells using trypan blue exclusion and cell proliferative assay (# Cat. no. T8154, Sigma-Aldrich). The assay was performed for 10 d post-seeding i.e. ...
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bioRxiv - Genomics 2022Quote: ... and 40 h later treated with 2 μg/mL doxycycline hyclate or seeded in 96-well plates at 3e3 cells per well and 24 h later treated with 1 μg/mL doxycycline hyclate and either DMSO or 10 μM MG132 (Sigma-Aldrich). Caspase 3/7 activity was measured 16 and 24 h later using the Caspase-Glo 3/7 Assay System (Promega ...
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bioRxiv - Bioengineering 2022Quote: ... Filtered particles were either immediately introduced to cells (105 target cells plated in a 6-well plate) in antibiotic-free culture medium supplemented with 8 μg ml-1 polybrene (TR-1003-G, EMD Millipore) or stored in single use aliquots at −80ºC ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... 17504044)] and counted using a hemocytometer and plated at appropriate densities onto chambers or wells that were incubated with laminin (Sigma, L2020). CGNPs were cultured at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 10 μl of a 100-fold dilution of beads–protein was incubated 2h at 37°C with 100μl 900-fold diluted plasma samples before addition of THP-1 cells (25,000 cells per well; Millipore Sigma, Burlington, MA). After 19h incubation at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... cells seeded in 12-well plates were pre-treated with 500 mU/mL α2-3/6/8 sialidase from Clostridium perfringens (Sigma-Aldrich) for 3 hours at 37°C before infection ...