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Citations for Millipore Sigma :
8251 - 8300 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Plasmids encoding shRNA against SYNJ2BP and AMPK α1 and α2 (TRCN0000139049, TRCN0000024000 and TRCN0000024046, respectively) as well as a control shRNA plasmid (TR30021) were purchased in pLKO from Sigma-Aldrich. PINK1-kinase dead-MS2-PP7 ...
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bioRxiv - Genetics 2023Quote: ... Tissues were then transferred into an enzymatic digestion buffer (200 µL, in a 24-well plate) containing Dispase II (2 mg/mL, Sigma Aldrich) and Collagenase Type P (0.4 mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... 1×105 cells were plated on a 12-well cell culture multiwell plate containing poly-D lysine-coated coverslips (Sigma-Aldrich). After 24 hours ...
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bioRxiv - Immunology 2023Quote: ... was added to the transfected culture to a final concentration of 4 mM as well as antibiotic-antimycotic solution (Sigma-Aldrich). The supplemented culture was further maintained for 6 days before the harvest of the cell supernatant ...
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bioRxiv - Microbiology 2023Quote: ... wells were incubated with anti-mouse IgG peroxidase-conjugated (1:1000 in PBS + 1% BSA, Sigma Aldrich, St. Louis, MO, USA) at RT for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were grown on coverslips placed in 24-well plates and treated with 10 μM of MG132 (M7449-200UL, Sigma Aldrich) diluted in fresh DMEM for 4 h followed by fixation with 4% PFA (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... cell colonies were grown on Matrigel-coated 6-well plates (Matrigel: Cat No. 354277, Corning; Plates: Cat No. CLS3516-50EA, Sigma Aldrich) in mTeSR Plus for at least three passages before being dissociated for downstream differentiation protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were seeded in 15-well IBIDI angiogenesis chambers during passaging and fixed 7-12 days later in 4% paraformaldehyde (PFA) (Sigma-Aldrich) for 60 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... for medium was refreshed for all cells and MCF7 cells were transfected with 2µg pcDNA3-PRB per well using X-tremeGENE HP DNA Transfection Reagent (Sigma Aldrich, #06366546001) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Human neuroblastoma SH-SY5Y cells (1 ×105) were seeded into a 24-well plate coated with poly-D-lysine (p6407, Sigma-Aldrich) one day before pseudo-lentiviral particle transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×105 target tumour cells were seeded in 6 well plates and infected with lentiviruses + polybrene (8 µg/mL) before selection of transduced cells using 4ug/mL puromycin (Sigma-Aldrich).
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bioRxiv - Biophysics 2023Quote: ... Observation wells were then washed with 5x50 μl KG7 and 50 μl imaging buffer (KG7 containing 2 mM Trolox (Sigma-Aldrich), 1 mg/ml glucose oxidase (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: The 12-well plates used for tumor sphere formation were pre-treated with 12 mg/ml poly HEMA (SIGMA, Catalog # P3932) and dried on a clean bench ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... The nodose ganglia were then transferred to a well containing 1 ml HBSS- and 100 µl Trypsin (Sigma; T9935-50 mg) and were incubated 5 min at 37°C ...
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bioRxiv - Physiology 2023Quote: ... Peroxidase-Glucose oxidase (PGO) enzyme plus color reagent was added to each well (Glucose Assay Kit: Sigma GAG020, PGO Sigma P7119), the plate was incubated at 37°C and the optic density was read at 450 nm using a Tecan Spark Microplate Reader ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... each well was coated overnight at 37°C with 1 mL of 100 μg/mL poly-L-ornithine hydrobromide (PLO, Sigma, P3655) prepared in filter sterilized 0.1 M borate buffer (Fisher Chemical ...
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bioRxiv - Cell Biology 2023Quote: Cells cultured in the 24-well plates in ESTEM-HE medium were treated with 100 nM vitaminD3 (D1530-10UG, Sigma-Aldrich)or 40 μM β-naphthoflavone (N3633-1G ...
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bioRxiv - Bioengineering 2023Quote: ... was then added to the construct and the posts containing the muscle construct was removed from the agarose mould and placed into a new 12-well plate with myoblast medium containing 33 µg/µl aprotinin (Sigma, A3428) at 37°C ...
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bioRxiv - Physiology 2023Quote: ... Peroxidase-Glucose oxidase (PGO) enzyme plus color reagent was added to each well (Glucose Assay Kit: Sigma GAG020, PGO Sigma P7119), the plate was incubated at 37°C and the optic density was read at 450 nm using a Tecan Spark Microplate Reader ...
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bioRxiv - Immunology 2023Quote: ... One well per NHP was stimulated with 10 pg/mL phorbol myristate acetate (PMA) and 1 μg/mL ionomycin (Sigma-Aldrich) (P+I ...
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bioRxiv - Genomics 2023Quote: ... Then cells in each well were washed with PBS carefully once and the fresh medium containing 10mM uridine (Sigma U3750-1G) was added ...
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bioRxiv - Bioengineering 2022Quote: ... 650 μl of the stromal gel mix was cast on 24-well plate (Corning® Costar ®, Sigma-Aldrich, Dorset, UK). The tumour mass was placed in the middle of the first stromal layer and covered by a second stromal layer of 650 μl of the stromal gel mix.
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bioRxiv - Bioengineering 2023Quote: ... K562 or Jurkat T cells were seeded into a 24-well plate at 2.5 × 105 cells/well in 1 ml cRPMI and transduced in the presence of protamine sulfate (8 μg/ml, Sigma-Aldrich). Transgene expression was assessed 72 hours post-transduction by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x 104 cells/well were seeded on a PLL coated (25 μg/ml Poly-L-lysine hydrobromide (Sigma-Aldrich: P1274)) 96 well plate ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was harvested every 4 h over a 48-h period using 0.25 ml/well of Trisole Reagent (Sigma-aldrich: T9424). For reverse transcription reactions ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp. ...
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bioRxiv - Neuroscience 2023Quote: ... The protein extracts were loaded in equal amounts into the wells of a 10% SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (EMD Millipore). The blots were incubated overnight with primary antibodies at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary cells of BC tissues were concentrated in six-well plates coated with 2% poly-2-hydroxyethyl methacrylate (Sigma-Aldrich) at a density of 1×104 cells/ml in serum-free DMEM/F12 (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: Virus medium mixed with fresh medium (1:1) was added to 10cm or 6-well plates with 10 µg/mL polybrene (TR-1003, Sigma Aldrich). 24 hours after infection the medium was discarded and changed to fresh medium ...
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bioRxiv - Systems Biology 2023Quote: ... single cells were sorted based on their GFP signal into 384-well plates containing 100 nl of unique CEL-seq2 compatible primer (7 ng/µl) and 5 µl mineral oil (Sigma-Aldrich). Following cell lysis and denaturation ...
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bioRxiv - Cell Biology 2023Quote: ... samples were aspirated from each well and kept on ice for analysis with a colorimetric glutamate assay kit (Sigma-Aldrich; MAK004), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Successfully injected blastocysts were transferred to a 60ml center well organ culture dish containing CO2-buffered culture medium (EmbryoMax KSOM medium (MR-106-D, Sigma-Aldrich) for mouse blastocysts ...
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bioRxiv - Cell Biology 2023Quote: ... OM SVF Lin–/TM4SF1+/MSLN– cells as well as cells subjected to siRNA-mediated knockdowns 48h post-transfection were collected into TRIzol (Sigma, #T3934). The direct-zol RNA kit (Zymo Research #R2062 ...
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bioRxiv - Immunology 2023Quote: ... Extracellular nitrite released from BMDMs (1×105 cells/well) stimulated with either HK-ST (MOI = 10) or 100 ng/ml LPS (Sigma, #L7895) was measured using a colorimetric assay based on the Griess reaction (Green et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 24-well glass-bottomed plates coated with Poly-L-ornithine/laminin (15 µg/mL Sigma Aldrich 10 µg/mL Sigma Aldrich), respectively ...
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bioRxiv - Microbiology 2023Quote: Cells were seeded the day before into 24-well plates containing sterile glass coverslips coated with poly-L-Lysine (Sigma-Aldrich). The seeding density per well was 150,000 cells for HEK293T and 50,000 cells for U2OS and Huh7 cells ...
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bioRxiv - Neuroscience 2023Quote: ... cells were suspended in plating medium either in 6-well plates coated with 0.05 M poly-D-lysine (Sigma, Cat# P7280) diluted in 0.05 M borate buffer (for protein extraction ...
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bioRxiv - Cell Biology 2023Quote: ... Dissociated neurons were then resuspended in warm DMEM supplemented with 10% FBS and plated in 6-well plates containing 25-mm sonicated glass coverslips pretreated with 50 μg/ml poly-L-lysine (PLL, Sigma-Aldrich). After 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... The inocula were removed from the wells and replaced with serum-supplemented medium containing 25 µg/mL of gentamicin (Sigma-Aldrich), to kill non-internalized bacteria ...
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bioRxiv - Microbiology 2023Quote: ... cells were seeded on coverslips in 12-well plate and challenged with 100 pg/ml LPS (Sigma, L4391; diluted in PBS) for 12 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were transduced for 48 h in 6 well plate in 1 ml of medium containing 8 µg/mL of polybrene (Sigma-Aldrich). VHL-NbGFP4-FLAG HelaS3 cell line was obtained after selection with 1 µg/mL of puromycin (Invivogen).
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bioRxiv - Bioengineering 2023Quote: ... Matrigel was first digested using 3 ml of dispase per well (PluriSTEM™ Dispase-II, 1 mg/mL, Millipore Sigma, SCM133) and incubated for 10 min in a 15 ml conical tube at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... substrates were placed in the 6-well plates and they were coated with poly-l-lysine (PLL, Sigma-Aldrich, MO, USA) solution prepared in ddH2O ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was gently disaggregated and neurons plated for 90 minutes with DMEM 10% HS medium in P6 well plates previously treated with 1 mg/ml poly-L-lysine (Sigma Aldrich) at a density of 300,000 cells/cm2 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 µg/mL, Sigma-Aldrich, St. Louis, MO) and cultivated in DMEM medium containing 10% FCS and 2 mM L-glutamine at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 4a-e, 4g, 4j, 5d-f) were cultured in 24-well plates (Falcon, 353047) on mixed cellulose ester Millicell inserts (Millipore, PIHA01250). Cells were plated at 100,000 cells/insert and cultured 6-8 weeks before experimental manipulations ...
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bioRxiv - Neuroscience 2023Quote: ... the neurons were plated in 4-well plates coated with poly-L-lysine (1 mg/ml) (Cat. #: P2636-500MG, Sigma-Aldrich) and homemade laminin (Chauvet et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then centrifuged at 200 rcf for 5 min and resuspended at a density of 2×105 cells/ml in ultra-low attachment 6-well plates (Sigma, CLS3471) using N2B27 medium (50% Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... and plated (1×106 per well) in M-199 medium and incubated for 5 min with 10 μg/mL propidium iodide (PI) (Sigma-Aldrich) to label the non-viable parasites ...