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Citations for Millipore Sigma :
8101 - 8150 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and corresponding secondary antibodies as well as the nuclear dye Hoechst and fibrillar Aβ dye Thiazine red (ThR 2 μM, Sigma Aldrich). Full sections were analyzed by tile scan mode on a Leica SP5 II confocal microscope ...
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bioRxiv - Biochemistry 2020Quote: ... Identical microarrays were printed on 16 isolated wells in each pGOLD biochip with a total of 4 biochips resembling a 64-well plate using the FAST frame incubation chamber (Millipore Sigma). The SARS-CoV-2 antigen-printed biochips were vacuum sealed and stored at −80 °C until use.
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bioRxiv - Neuroscience 2021Quote: ... the electrode surfaces of 6-well Axion plates (Axion Biosystems, CytoView MEA 6) were coated with 10 mg/mL poly-D-lysine (Sigma, P7280) at room temperature overnight ...
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bioRxiv - Neuroscience 2021Quote: TEER across cellular monolayers was measured with chopstick electrodes in 24-well Transwell inserts using the Millicell® ERS-2 Voltohmmeter (Merck Millipore). The absolute TEER of eGFP-hCldn5-MDCK II cells before treatment was recorded as a baseline reading ...
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bioRxiv - Cancer Biology 2021Quote: ... each NBC population was thawed into a 6-well plate and infected with a unique barcode-containing lentivirus supernatant in medium with Polybrene (Sigma Aldrich) at a concentration range from 1:200 to 1:50 ...
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bioRxiv - Cancer Biology 2021Quote: ... Coring 6.5 mm Transwell with 8.0 um pore polycarbonate membrane insert in 12 well plates were used (Sigma Aldrich, cat# CLS3422). The insert membrane was treated for optimal cell attachment ...
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bioRxiv - Biophysics 2021Quote: ... The glass surface was blocked prior to GUV addition by incubating the wells with 200 μL 2g/L BSA (Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... 40,000 and 80,000 cells) of the above-mentioned cell lines were seeded in duplicate into a 48-well plate (Corning Costar, Sigma-Aldrich) required for the EpiQ chromatin assay ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcriptase qPCR was performed in a Framestar-480/384 well plate containing 5 µl of 2x SYBR Green JumpStart Taq ReadyMix (Sigma-Aldrich), 2 µl cDNA ...
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bioRxiv - Biochemistry 2021Quote: ... All wells were then incubated with 3,000-fold diluted goat anti-human IgG-alkaline phosphatase conjugate (Sigma-Aldrich, St. Louis, MO) for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... alkaline phosphatase staining was completed within each well using a commercially available Leukocyte Alkaline Phosphatase staining kit (Sigma Aldrich 86R-1KT). Whole well images were collected through brightfield microscopy and quantification of colony forming units (CFUs ...
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bioRxiv - Microbiology 2020Quote: ... The wells were further blocked with 15 µl of 10 µM biotin in 3% skim milk in PBST (Sigma, cat# 1.15363.0500) for ten min at R.T ...
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bioRxiv - Neuroscience 2021Quote: ... Three wells of BMEC-like cells per condition were then lysed in PBS containing 5% Triton X-100 (Sigma-Aldrich #T8787), and one well per condition was fixed in ice cold 100% methanol ...
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bioRxiv - Neuroscience 2020Quote: ... and the organ was cultured in a well on a cover glass coated with 1:1 poly-L-ornithine (0.01%; Sigma-Millipore, USA) and laminin (50 μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... The middle region of the cochlea was cultured in a well on a cover glass coated with 1:1 poly-L-ornithine (0.01%; Sigma-Millipore #P4957, USA) and laminin (50 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... of a nucleic acid extract of each water type as well as into reagent-grade water (Milli-Q, Water Purification System, Millipore, Ontario) to serve as a positive control and subject to PCR as described above ...
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bioRxiv - Microbiology 2020Quote: ... from the well were filter using a sterile membrane filter (pore size 0.22 μm, GV CAT No GVWP04700, Millipore, Darmstadt, Germany). The filters were collected and then resuspended in Dorn mineral medium [50] with methanol 25 mM as a sole source of carbon and energy ...
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bioRxiv - Cancer Biology 2020Quote: ... 90 μL phenol red free DMEM supplemented with 10% FBS was added to each well and followed with the addition of 10 μL of diluted MTT reagent (Sigma-Aldrich, UK ...
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bioRxiv - Cell Biology 2021Quote: HUVEC were seeded at 5×104 cells or 2×105 cells in 24-well plates on coverslips coated with 10 μg/ml fibronectin (from human plasma, Sigma Aldrich) and incubated overnight in complete EBM-2 medium ...
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bioRxiv - Cancer Biology 2021Quote: ... viroid’s containing open reading frames of dCas9-VP64 and MS2-P65-HSF1 as well as target specific guideRNA expression constructs were purchased (SAMVP64BSTV, SAMMS2HYGV and LV06 respectively, Sigma Aldrich) and used to stepwise transduce and select PC-9 cells ...
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bioRxiv - Cell Biology 2021Quote: HEMs or MNT1 cells seeded in a 6-well plate at 50-80% confluency were lysed in 1% Triton X-100 (T8787, Sigma-Aldrich) and centrifuged at 14,000 RPM for 30 min at 4°C to separate solubilized protein from melanin ...
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bioRxiv - Cell Biology 2021Quote: ... media was removed from wells and cells were incubated with MTT from the Cell Proliferation Kit (Sigma Aldrich, catalogue no. 11465007001), and the colorimetric assay was performed following manufacturer’s directions ...
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bioRxiv - Cell Biology 2022Quote: CD15+ neutrophils were seeded at 20,000 cells per well in in HBSS with or without 15 μM Cytochalasin D (Sigma Aldrich, Burlington) black walled 96 well plates (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μL of PsVs was added to each well and incubated for 48-60 hours in the presence of 10 μg/mL of polybrene (Sigma Aldrich). Unless stated otherwise ...
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bioRxiv - Microbiology 2022Quote: ... cells/cm2 in a 24-well plate with 2 wells for 0.1% BSA/PBS treatment and 2 wells for 500 U/mL IFN-ß (dissolved in 0.1% BSA/PBS, Millipore Sigma IF104) treatment ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell count of three random wells was taken every day up to 5 days using trypan blue (Cat # T6146, Sigma, U.S.A). The doubling time was calculated using the equation - Td = TX log2/log(N/N0) ...
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bioRxiv - Bioengineering 2022Quote: ... The two-post array supporting the newly formed hydrogels was then lifted out of the casting plate and transferred to a fresh 24-well plate containing Lonza growth medium supplemented with 2 g/L aminocaproic acid (Sigma-Aldrich) to inhibit fibrinolysis ...
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bioRxiv - Bioengineering 2022Quote: ... a 96-well plate was seeded with 1×104 L929 murine fibroblast cells per well and incubated for 24 h to allow for cell adhesion and spreading (L929 cell line was purchased from Sigma-Aldrich, Poznań ...
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bioRxiv - Bioengineering 2022Quote: ... the top well of m-μsims was coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma Aldrich) and human fibronectin (5 µg/cm2 ...
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bioRxiv - Immunology 2022Quote: 8-well Nunc Lab-Tek II chambered coverglass (Thermo #155360) were cleaned by submersion in 5% Hellmanex III (Sigma-Aldrich #Z805939). Flask containing chamber in solution was microwaved for 25 sec and then allowed to clean at room temperature overnight ...
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed and wells filled with DMEM supplemented with 10% FBS and antibiotics to induce D2R expression (Hygromycine 2 μl/ml (Sigma Aldrich); Blasticidine 1,5 μl/ml (Cayla Invivogen ant-bl-1) ...
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bioRxiv - Microbiology 2020Quote: ... 24-well plastic plates were seeded with THP-1 cells at a density of 30 ⨯ 104 cells per well in complete medium after being treated with 30 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich). These 24-well plastic plates where then incubated for 48 hs to induce maturation mediated by PMA toward adherent macrophage-like cells ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were collected in PBS in 24-well plates and processed for free-floating immunocytochemistry using primary polyclonal antibodies of phosphorylated Tau pSer214 (, rabbit, 1:1000 Sigma, USA) and NOS (Cayman Chemical,1:1000 ...
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bioRxiv - Neuroscience 2021Quote: The resultant dissociated endothelial cells were washed in working buffer and plated at 500,000 cells/well on collagen IV and fibronectin (100µg/ml and 50µg/ml respectively; Sigma-Aldrich; overnight, 4°C) coated coverslips in a 24-well plate in 1ml Endothelial Growth Media-2 Bullet Kit (EGM-2 ...
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bioRxiv - Cell Biology 2021Quote: U2Os cells were transfected with EB1-mNeon and seeded on poly-D-lysine−coated borosilicate cover glass in six-well culture plates (Sigma-Aldrich) 24 hr prior to imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... a single fluorescent cell was sorted per well of a fibronectin-coated (10 μg/mL Human Plasma Fibronectin Purified Protein (Sigma Aldrich) in 1x PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 48-well MEA plates consistent of 16 electrodes per well were precoated with 0.1-mg/ml poly-D-lysinehydrobromide (PDL SIGMA #P7886-50MG) overnight (ON ...
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bioRxiv - Microbiology 2020Quote: ... We grew donor strains in 96-well deep-well plates (1-mL cultures in TY medium in 2-mL wells) for at least 5 h shaking at 37 °C with a Breath-easy (Sigma-Aldrich) film covering the plate ...
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bioRxiv - Microbiology 2020Quote: ... 48-well plate) were infected with varying dilutions of viral stocks in the presence of 8 μg/mL polybrene (Sigma Aldrich). 48 h following infection ...
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bioRxiv - Immunology 2021Quote: ... coated channels of a 48-well microfluidic plate (Bioflux, Fluxion Biosciences) and then injured by flowing a 50ug/mL solution of hematoporphyrin (Sigma-Aldrich) with exposure to blue light using the HXP-120 C light source with 475-nm excitation and 530-nm emission filters as previously described (51) ...
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bioRxiv - Microbiology 2020Quote: ... A volume of 100 μL of inoculum was then added to each well containing 100 μL of two-fold serial dilutions of PZA (Sigma, P7136) ranging from 4 to 512 mg/L in complete Middlebrook 7H9 broth ...
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bioRxiv - Microbiology 2021Quote: ... Tissues in each well were resuspended in 1.2 mL of a commercial DMSO-free cryopreservation medium (CryoSOfree™, C9249; Sigma-Aldrich), divided into 200 μL aliquots ...
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bioRxiv - Cancer Biology 2020Quote: ... The ports of the sensor cartridge were sequentially loaded with 20 μl per well of the appropriate compound: the ATP coupler oligomycin (Sigma, O4876), the uncoupling agent carbonyl cyanide 4-(trifluoromethoxy ...
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bioRxiv - Neuroscience 2021Quote: Cortical synaptosomes were incubated for 90 min at 4 °C with gentle shaking on Nunc Lab-Tek 8-well chamber slides (Sigma-Aldrich) coated with poly-D-lysine (ThermoScientific) ...
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bioRxiv - Microbiology 2021Quote: ... The cells were dissociated from the brush by gentle agitation before seeding into a single well of a collagen (PureCol from Sigma Aldrich) coated plate ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were plated on polyester membrane inserts (0.4 μm pore) in 6 well culture plates (Corning Costar CLS3450-24EA, Sigma-Aldrich, UK), 2-3 slices per insert ...
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bioRxiv - Systems Biology 2022Quote: ... OP9 cells expressing PPARγ-mCitrine were plated at a density 10,000 cells/well on a fibronectin-coated (10ug/ml) (Sigma Aldrich, F0895) 96-well glass imaging plate (Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... or Forskolin as described above then cells were trypsinized and resuspended in drug containing media at 30,000 cells per well in a XF cell plate coated with 0.05% poly-L-lysine then incubated overnight (Sigma-Aldrich, 4707).
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bioRxiv - Genetics 2022Quote: ... coated slides containing 12 wells (Carlson Scientific cat# 101206) were treated by pipetting 30 μl 0.01% poly-L-lysine (Sigma Cat# p4707) into each well and incubating at RT for 15 min ...