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Citations for Millipore Sigma :
8051 - 8100 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: NKX3.1 expression was inhibited using siRNA against the NKX3.1 gene (WD10664038) as well as universal negative control SiRNA#1 (SIC001) was purchased from Sigma Aldrich. 70-90% of confluent cells were replaced with Opti-MEM ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... at a density of 80,000 cells per well after pre-coating with 25 µg/mL fibronectin (Cat: F1141, Sigma Aldrich). Cells were treated with specified concentrations of MβCD for 75 minutes in 0.2% BSA medium ...
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bioRxiv - Cancer Biology 2023Quote: 1×105 U2OS IRE1-HaloTag cells were grown on 24 h in Millicell EZ Slide 4-well chamber slides (Millipore), fixed in 4% PFA and permeabilized with 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... and the cells were plated at a density of 75,000 per well of 13 mm diameter onto glass coverslips previously coated with poly-L-lysine (10 µg/mL, Sigma). Cultures were maintained in neurobasal medium supplemented with B-27 ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then washed 3x with 200μLs of PBS-T per well and anti-mouse IgG HRP-conjugated secondary (Sigma, #AP308P) diluted at 1:3000 in blocking solution was added to each well and incubated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... and plated in 24-well plates containing 12-mm glass coverslips pretreated with 50 µg/ml poly-L-lysine (Sigma) in Borate buffer (1.24 g boric acid (Fisher) ...
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bioRxiv - Biophysics 2023Quote: ... 50 µL/well of a 37 °C prewarmed alkaline phosphatase yellow (pNPP) liquid substrate (Sigma-Aldrich, Cat # P7998, Lot # SLCJ1764) was added ...
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bioRxiv - Cell Biology 2023Quote: ... and plated in 24-well plates containing 12-mm glass coverslips pretreated with 50 µg/ml poly-L-lysine (Sigma) in Borate buffer (1.24 g boric acid [Fisher] ...
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bioRxiv - Cell Biology 2023Quote: 2×10^5 Vero E6 cells or HEK293-ACE-2 were cultured in 24-well plates in Dulbecco’s Modified Eagle medium (DMEM, Sigma D5796) containing 10% of fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2024Quote: ... of 6 mm in diameter and 3 mm in thickness at a density of 2 × 106 cells per scaffold in 12 well plates coated with 1 % agarose (Sigma) for chondrogenic differentiation and in a 12-well plate for osteogenic differentiation ...
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bioRxiv - Cell Biology 2024Quote: ... and then seeded at 2000 cells per well in enriched pseudoislet media supplemented with 2 μM 4-hydroxytamoxifen (4-OHT; Sigma) or vehicle control (0.1% ethanol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The wells were washed four more times and 200 μL of OPD (o-phenylenediamine dihydrochloride) substrate (P8412 Sigma-Aldrich, France) was added in each well ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated (10-30 minutes) with 200 μL of aqueous color reaction buffer: 0.1M Citric acid (#C0759, Sigma-Aldrich), 0.2M Na2HPO4*2H2O (#71643 ...
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bioRxiv - Cancer Biology 2024Quote: ... were diluted in pre-warmed XF assay media to achieve the following final concentrations in the cell culture well: 1.5 μM Oligomycin A (Sigma, 75351); 1 or 0.5 μM FCCP (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... A549 shPARP14 as well as shp62 knockdown cells were generated in the lab using lentiviral shRNA constructs TRCN0000290897 and TRCN0000007237 (Sigma). All cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... cells in a single well were trypsinized and live cell count obtained by staining with 0.4% trypan blue (Sigma, T8154) and counted using the BioRad TC20 automated cell counter ...
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bioRxiv - Cell Biology 2024Quote: ... wells designated in the Torin-1 treatment arm were replaced with fresh complete medium containing 1µM Torin-1 (Sigma #475991), and treated for 4 hours ...
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bioRxiv - Microbiology 2024Quote: ... 20 µL from each condition were sampled from 24-well plate and serially 10-fold diluted in 180 µL of phosphate-buffered saline (PBS) (Millipore) Then ...
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bioRxiv - Neuroscience 2024Quote: 1x105 cells/well primary striatal astrocytes and iHA were plated onto 12mm glass coverslips (Thermo-Scientific) in 24-well plates coated with Poly-lysine (Sigma) or in 50% laminin 111 (LN111 ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 10,000 cells were seeded into 12-well plate pre-coated with 100 μg/ml poly-D-lysine (Sigma Aldrich) and 10 μg/ml laminin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were plated in in 24-or 6-well plates previously coated with Poly-L-ornithin (0,1 mg/ml, Sigma-Aldrich) at a density of 250,000 cells/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... A 96-well plate was filled up with 200 µl per well of XTT salt solution (1mg/ml in PBS) and menadione solution (1 mM in acetone; Sigma) and incubated for 3 h at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: After a well-fed gravid hermaphrodite was immobilized in the egg salt solution (ESS) with 10% tetramisole (Sigma, St. Louis), a cut was made across the vulva using a 26G subcutaneous needle controlled by a micromanipulator (ROE-200 ...
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bioRxiv - Physiology 2024Quote: ... tardigrades were transferred to a glass dish without agar (Agar Scientific) and were washed well with sterilized Milli-Q water (Millipore). Then ...
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bioRxiv - Plant Biology 2023Quote: ... the slides were mounted in a plastic cassette and sections incubated in pre-permeabilization solution21 (48 μl Exonuclease I buffer, NEB, cat no. B0293S; 4.5 µl of BSA, Sigma-Aldrich, cat no. A7039-100G; and 2% (w/v) PVP40 ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then collected and re-plated at 500 cells/well with either 5 μM 4-nitroquinolone N-oxide (4NQO) (Sigma, N8141) or a dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2021Quote: ... hCEcto stem cells were grown on collagen-coated six well-plate to 50% confluence before they were treated with virus solution and Polybrene (1μg/mL) (Sigma-Aldrich, # H9268) overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... ARPE-19 cells were seeded at semi-confluence in 12-wells plates and next day medium was replaced for growth medium containing 2mM thymidine (Sigma-Aldrich). After 24 hrs ...
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bioRxiv - Cancer Biology 2021Quote: ... SMF cells and EMT6 cells were transduced at 50% confluency in 6-well plates with 1 mL of concentrated lentivirus and 6 µg/mL polybrene (Sigma 107689) at 1000xg and 33°C for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Egg chambers of interest were then transferred into a mutli-well plate with 500 μl fresh media with the addition of MG132 (EMD Millipore) or DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed once with PBS and lysed by adding 250 μl/well of 0.1 % Igepal CA-630 (Sigma-Aldrich, I8896) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 24 well plate) to the adherent osteoblast precursors in presence of DM-medium supplemented with 1 µM PGE2 (Sigma-Aldrich, #P6532) and 10 nM 1,25(OH)2D3 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: Cells were cultured on a coverslip place onto a 48-well plate and after 48 hours they were washed with PBS and fixed with 4% Paraformaldehyde (PFA) (Sigma Aldrich) for 30 minutes at RT ...
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bioRxiv - Immunology 2021Quote: ... 50 μl/well of 3,3’-5,5’-tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (T040, Sigma-Aldrich Co. LLC., St. Louis, MO) was added to the plate ...
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bioRxiv - Developmental Biology 2020Quote: For virus titration in a relevant cell type, neural NE4C cells (ATCC, CRL2925™) were cultured on a 6-well plate in MEM (M5650, Sigma) supplemented with 10% FBS and 1% Pen/Strep ...
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bioRxiv - Cancer Biology 2022Quote: ... 6×105 cells were infected in 6-well plates with 125uL of high titer virus in standard media with 5ug/mL Polybrene (Sigma-Aldrich). Media was changed after 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... or cells with non-targeting or LDLR targeting gRNAs were seeded on 96-well plates as 4000 cells/well and treated with GSK-J4 in the presence or absence of exogenous cholesterol the next day (250µM, Sigma-Aldrich, USA). Cell viability was measured after 72h of the treatment.
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bioRxiv - Neuroscience 2021Quote: ... GCaMP6f-expressing HEK cells were seeded on 12-well cell culture plates with 18mm glass coverslips coated with PDL (10μg/μl; Sigma-Aldrich P6407) for 1-2h ...
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bioRxiv - Molecular Biology 2020Quote: ... The embryos were extracted from gravid adults by bleaching and the eggs were introduced into the well of a corning plate (CLS3513, Sigma Aldrich) containing OP50 at an OD of 1.0 diluted in S-basal medium (For 1 Liter ...
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bioRxiv - Genomics 2020Quote: ... The complete reaction mix was again removed and a slide wash with one 10 minute wash of the wells with 2X SSC/0.1% SDS (Sigma-Aldrich, USA), followed by 1 minute wash with 0.2X SSC and finally 0.1X SSC was performed ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed six times and incubated with 100 μL/well horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Fc-specific, Sigma Aldrich) diluted 1/5000 in PTA buffer ...
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bioRxiv - Systems Biology 2019Quote: ... Streptavidin-biotin complexes were further generated by adding 1 μL/well of 1 mg/mL of streptavidin solution (Sigma-Aldrich, #85878). The reconstituted luciferase enzymatic activity was monitored by injecting 50 μL/well of the Nano-Glo reagent (Promega ...
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bioRxiv - Cell Biology 2019Quote: COS-7 or HeLa cells grown in 6-well plates were cultured in the presence or absence of doxorubicin (DOX) (44583: Sigma-Aldrich) or Actinomycin D (AcD ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed and developed for 15 min with 100 μl/well of a Sigmafast p-nitrophenyl phosphate solution (Sigma-Aldrich). Plates were read at 405nm ...
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bioRxiv - Cell Biology 2019Quote: ... 4,000 cells per cm2 in passage 2 were seeded on a 12 well plate coated with fibronectin (2 μg/cm2; Sigma Aldrich) and allowed to grow confluent ...
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bioRxiv - Biochemistry 2019Quote: ... After 6 or 7 days cells were resuspended in enzyme-free EDTA buffer and replated in untreated 6-well plates (BD Falcon, Sigma-Aldrich) at 1 M cells per well in unconditioned medium with or without priming in 25 U IFN-γ (PeproTech ...
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bioRxiv - Biochemistry 2019Quote: ... After 24 h cells were resuspended in enzyme-free EDTA buffer and replated in untreated 6-well plates (BD Falcon, Sigma-Aldrich) at 1 M cells per well in unconditioned medium ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with PBST and 100 μl/well of goat anti-human immunoglobulins-peroxidase IgG (FC specific; Sigma-Aldrich), IgM (μ-chain specific ...
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bioRxiv - Microbiology 2019Quote: ... The cell pellet was resuspended in about 800 μL of 1x M2 salts and mixed well with percoll (900 μL, Sigma Aldrich). Swarmer cells (bottom layer ...
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bioRxiv - Genomics 2021Quote: ... and sorting G1 cells based on Hoechst staining on an Influx FACS machine into 384 well plates containing 5 μl sterile filtered mineral oil (Sigma Aldrich) per well ...