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Citations for Millipore Sigma :
8201 - 8250 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... faecalis wild-type OG1RF and OG1RF ΔmptD were sub-cultured to OD600 of 0.01 in a 24-well microtiter plate containing 1 mL of Tryptone Soya Broth without dextrose (TSBd, Sigma-Aldrich, USA) supplemented with or without mannose (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... The apical bud-internode unit along with each of the next 23 axillary bud-internode units was placed in a well with 1 ml of water as treatment control or 1 µM GA3 (Sigma-Aldrich) solution ...
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bioRxiv - Neuroscience 2022Quote: ... Micro-islands were generated as described previously (Meijer et al., 2012) by plating 8000/well rat glia on UV-sterilized agarose (Type II-A; Sigma, A9918) coated etched glass coverslips stamped with a mixture of 0.1 mg/ml poly-D-lysine (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated in 24-,12- or 6-well plates pre-coated with 10 μg/ml of poly-L-lysine (Sigma-Aldrich) and maintained at 37°C in a humidified atmosphere containing 5% CO2.
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bioRxiv - Neuroscience 2022Quote: HEK293 cells stably expressing FLAG-tagged D3R were grown in DMEM with 10% FBS in 6-well plates coated with 400 µl ECM (Sigma E1270) to 80-90% confluence ...
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bioRxiv - Molecular Biology 2023Quote: ... 450 μL media was removed from each well and replaced with 500 μL complete neurobasal media supplemented with 10 μM 5-fluorodexoyuridine (FUDR, Sigma-Aldrich) to inhibit glial cell growth ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed in cold DPBS three times and lysed directly in the cell culture wells with RIPA lysis buffer supplemented with 1x protease inhibitor cocktail (Sigma-Aldrich). Lysates were cleared via centrifugation at 20,000 rcf at 4 °C for 10 minutes ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plates was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plate was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Immunology 2022Quote: ... neutrophils were plated in 24-well plates containing glass coverslips covered with 0.01% poly-L-lysine solution (Sigma-Aldrich; cat. P8920), fixed with PFA 4% at RT for 10 min ...
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bioRxiv - Genomics 2022Quote: ... K562 or Jurkat cell lines expressing CRISPRi effectors of interest were infected with lentivirus containing sgRNA expression vectors by centrifugation at 1000 × g for 1 h in 24-well plates in the presence of 8 µg/mL polybrene (Sigma-Aldrich). RPE1 ...
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bioRxiv - Neuroscience 2022Quote: ... containing 4 mL EM per well with either 0.1% DMSO (drug carrier alone) or 0.1% DMSO + 10 μM dexamethasone (Sigma-Aldrich, Cat# D1756) for 48 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured on poly-D-lysine-coated coverslips in 24-well plates and were differentiated by adding 10 µM all-trans retinoic acid (Sigma; R2625) on day 2 and conducting a media exchange to brain-derived neurotrophic factor (BDNF)-containing media (DMEM with 50 ng/ml BDNF ...
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bioRxiv - Neuroscience 2024Quote: ... Controls included the library internal negative controls ath-miR416 and cel-miR-243 as well as pooled non-specific siRNAs [60,61] that were transfected into cells and then either treated with DMSO (Sigma, D4540-500ML), with 0.7 µM CCCP ...
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bioRxiv - Cell Biology 2023Quote: ... Dissociated neurons were then resuspended in warm DMEM supplemented with 10% FBS and plated in 6-well plates containing 25-mm sonicated glass coverslips pretreated with 50 μg/ml poly-L-lysine (PLL, Sigma-Aldrich). After 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... The wells were subsequently washed by PBS and washed with crystal violet (CV. The wells were washed and solubilized with 95% of ethanol (Sigma Aldrich) prior to the biomass measurement at OD600nm by the Floustar Omega plate reader ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then centrifuged at 200 rcf for 5 min and resuspended at a density of 2×105 cells/ml in ultra-low attachment 6-well plates (Sigma, CLS3471) using N2B27 medium (50% Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... and plated (1×106 per well) in M-199 medium and incubated for 5 min with 10 μg/mL propidium iodide (PI) (Sigma-Aldrich) to label the non-viable parasites ...
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bioRxiv - Microbiology 2024Quote: ... Each well contained 1 µl fluorescent dye indicator SYPRO Orange (Sigma Aldrich, 5000 x in DMSO, 1:40 diluted with H2O), 1 µl of 5 mg/ml DptM and 18 µl of buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were counted using a Neubauer Chamber and seeded on coverslips into multi-well culture dishes (Falcon) pre-treated with 0.1% poly-D-lysine (Sigma-Aldrich, #P7280) at a density of 12.500 cells/cm2 in plating medium containing antibiotics (streptomycin/penicillin 100 µg/ml ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 24 h in 24-well plates in complete culture media supplemented with 50 ng/mL PMA and 1 μg/mL ionomycin (Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... Two hundred thousand cells per well were seeded into a 12-well plate pre-coated with poly-D-lysine (5 μg/ml) in 0.1 M borate buffer (Sigma-Aldrich, #P7280). Cultures were grown in an incubator at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... approximately 7×105 cells growing on 6-well plates were washed with PBS and harvested with Tri Reagent (Sigma Cat#T9424). Total RNA was extracted ...
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bioRxiv - Cancer Biology 2024Quote: 0.5 to 1 million cells were plated into 6-well plate and treated with 0.5 or 1 mg/ml Dox (Sigma-Aldrich #D9891) to induce FGFR4 overexpression or knockdown for 48 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... were inserted into 24-well culture plates (Falcon) and coated with 100 μg/mL poly-D-lysine solution (Sigma-Aldrich #1804) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were cultured in feeder-free conditions on tissue-culture treated 6-well plates (Falcon® 353046) coated with 0.1% gelatine (Sigma G1393), in KnockOut DMEM (Gibco™ 10829018 ...
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bioRxiv - Genomics 2023Quote: ... sections were incubated with appropriate secondary antibodies as well as directly conjugated primary antibodies at concentrations according to vendor’s guidelines with DAPI (Sigma Aldrich D9542) at a final concentration of 1μg/ml in Agilent Antibody Diluent Solution for 1 hour at room temperature and washed again three times for 5 minutes with Agilent Wash Buffer Solution ...
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bioRxiv - Microbiology 2024Quote: ... Greiner Bio-One 96-well U-bottom microtiter plates were inoculated with bacterial strains at an initial OD600 of 0.02 in a final well medium volume of 150 μL and sealed with a Breathe-Easy® sealing membrane (Sigma Aldrich). Inoculated plates were incubated at 37°C with 300 RPM shaking ...
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bioRxiv - Immunology 2024Quote: ... coated 15μ-Slide 8 well (ibid) and incubated for 2h at 37LJ in RPMI 1640 supplemented with 10% BSA (Sigma-Aldrich). Cells were fixed using 1% [w/v] paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2024Quote: ... for differentiation induction 100.000 cells were plated in a 6-well plate and maintained in complete media supplemented with 20μM retinoic acid (Sigma-Aldrich, R2625) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... 1×106 cells were seeded on 0.5 mg/ml poly-D-lysine-coated sterile 12-mm diameter coverslips or aligned 700-nm diameter nanofibers in 24 well plates (Sigma, Z694533). Growth medium was changed on days in vitro (DIV ...
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bioRxiv - Cell Biology 2024Quote: MSCs were seeded overnight on 24 or 6 well plates in normal α-MEM supplemented with 10% fetal bovine serum (FBS, Sigma), 2 mM glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... THP-1 cells were plated to 24-well plate at a concentration of 2 × 105 viable cells per well and incubated for 48 h in 1 ml/well of media supplemented with 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for differentiation into macrophages ...
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bioRxiv - Immunology 2024Quote: ... was added to a well of a 24 well plate and a 6.5 mm Transwell® Polycarbonate Membrane insert with a 5.0 µm pore (Costar #3421/Sigma # CLS3421-48EA) was added to each well ...
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bioRxiv - Immunology 2024Quote: ... cells were seeded in a 6-well plate and transduced with 10 μL of concentrated lentivirus in the presence of 10 μg/mL polybrene (Millipore-Sigma; H9268) and replaced with fresh media 24 hrs ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... The THA hydrogels were directly prepared in the wells and were incubated for 1 hour with endotoxin free H2O (Sigma-Aldrich). An endotoxin standard stock solution was prepared by adding endotoxin free H2O (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were seeded in 15-well Ibidi chambers after passaging and fixed 3-4 days later in 4% Paraformaldehyde (Sigma-Aldrich) for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... cells were plated at ∼0.25-0.5 ×106 cells per well the day before treatment and reactivated at 70-80% confluency with 5 μg/mL doxycycline (Millipore Sigma) and 1 mM sodium butyrate (Sigma) ...
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bioRxiv - Biophysics 2023Quote: Mechanically passaged hiPSCS were plated on 10 µg/mL Vitronectin XF in 6 well TC plates in 1 μM all-trans-RA (Cat. No. R2500-25MG, Sigma-Aldrich) to NutriStem® hPSC XF Medium (Growth Factor-Free)(Cat ...
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bioRxiv - Cell Biology 2024Quote: ... 106 THP-1 cells were transferred to each of the six-well plates and cultured with 100ng/mL phorbol 12-myristate 13-acetate (PMA) (P8139, Sigma-Aldrich) for 24h ...
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bioRxiv - Microbiology 2023Quote: ... the wells were filled with 200 µl fresh M63 media with or without 3 µM of human transferrin (Sigma-Aldrich #T1147) and incubated at room temperature for 30 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... set to “high” shake speed and linear shaking mode in a 24 well microtiter dish plate sealed with a Breathe-EasyⓇ transparent membrane (Sigma-Aldrich). For the data in Supplementary Figure 9 ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-L-Lysine and Laminin coating of 24-well plates was performed by 48 h incubation at 37°C using Tissue Culture water (Sigma, W3500) supplemented with 1 μg/ml Poly-L-Lysine (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then plated at a density of 30,000 cells/well onto coverslips coated with poly-D-lysine (0.1 mg/mL; Sigma, St. Louis, MO). One to three days later ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for the immune gene of interest as well as the housekeeping gene and 250 nM of the respective probes (Sigma Aldrich), 3.74 μl nuclease-free water (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmids encoding shRNA against SYNJ2BP and AMPK α1 and α2 (TRCN0000139049, TRCN0000024000 and TRCN0000024046, respectively) as well as a control shRNA plasmid (TR30021) were purchased in pLKO from Sigma-Aldrich. PINK1-kinase dead-MS2-PP7 ...
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bioRxiv - Genetics 2023Quote: ... Tissues were then transferred into an enzymatic digestion buffer (200 µL, in a 24-well plate) containing Dispase II (2 mg/mL, Sigma Aldrich) and Collagenase Type P (0.4 mg/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded on 6-well cell culture plate wells at a density of ∼2 x 105 cells/well with 2 mL of the culture medium containing 103 units/mL ESGRO Recombinant Mouse Leukemia inhibitory factor (LIF) (Millipore #ESG1107) and 2i (1.0 µM PD0325901 Toris #4423 and 3.0 µM CHIR99021 Wako #038-23101) ...
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bioRxiv - Neuroscience 2023Quote: ... cut into small explants as above using a tungsten needle and either cultured on poly-L-ornithine/laminin coated wells (both from Sigma-Aldrich) for growth cone morphology analysis or embedded in rat tail collagen (BD Bioscience ...