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Citations for Millipore Sigma :
8301 - 8350 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were cultured in feeder-free conditions on tissue-culture treated 6-well plates (Falcon® 353046) coated with 0.1% gelatine (Sigma G1393), in KnockOut DMEM (Gibco™ 10829018 ...
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bioRxiv - Microbiology 2024Quote: ... The wells were subsequently washed by PBS and washed with crystal violet (CV. The wells were washed and solubilized with 95% of ethanol (Sigma Aldrich) prior to the biomass measurement at OD600nm by the Floustar Omega plate reader ...
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bioRxiv - Neuroscience 2023Quote: ... 6-well plates in maintenance media (base culture media further supplemented with 150 µM ascorbic acid (AA, Sigma, cat. no A4544), 3 µM CHIR-99021 (Axon Medchem ...
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bioRxiv - Microbiology 2023Quote: The deep-well plate with the drugs and communities was sealed with an AeraSeal breathable membrane (Sigma-Aldrich, cat. No. A9224) and incubated anaerobically at 37 °C for 24 h ...
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bioRxiv - Genetics 2024Quote: ... The samples were then transferred to an enzymatic digestion buffer (200 µl, in a 24-well plate) containing Dispase II (2 mg/mL, Sigma Aldrich) and Collagenase Type P (0.4 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mM β-Mercaptoethanol as well as freshly added 0.1 % Triton X-100 and protease inhibitor (cOmplete ™ EDTA-free protease inhibitor cocktail, Sigma-Aldrich) and lysed via French Pressure Cell (Thermo IEC) ...
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bioRxiv - Bioengineering 2024Quote: ... The mouse-depleted tumor cells of NIBRX-1362 were plated at 1250 cells per well with 40 μL of DMEM+2%FBS (Gibco, 11965092; Sigma, F2442) in 384-well opaque plates (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... or 12-well (1x105 cells/well) plates for western blotting and quantitative real-time PCR (qRT-PCR) experiments or on 0.2% gelatin (Sigma Aldrich, G1890)-covered 96-well plate (Grainer Bio-One ...
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bioRxiv - Immunology 2024Quote: ... The wells were rinsed three times with PBS containing 0.05 % Tween 20 and horseradish-peroxidase-labeled rabbit anti-human or anti mouse IgG (100 μL/well; Sigma-Aldrich, Germany) was added to each well and incubated for 1 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... EBs at day 5 were transferred to a new 6-well plate coated with PDL (Millipore. Catalog. MILL-A-003-E)/Laminin (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were seeded at a density of 30000 cells/well for regular experiments or 125,000 cells/well for transfection experiments in 4-well chamber slides (Lab-Tek II, 155382) coated with poly-L-lysine (20 µg/mL, Sigma, P1399) and laminin (2 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded at a density of 0.5 × 106 on 6-well plates precoated with 50 μg/mL poly-L-ornithine (Sigma #P-3655) and 1 µg/mL fibronectin in DMEM F12 with 1 % N-2 supplement (Invitrogen #17502048) ...
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bioRxiv - Neuroscience 2024Quote: ... 500’000 cells per well were seeded as single cells onto new 6-well plates (6 WPs) coated with vitronectin and cultured in mTESR+ medium supplemented with thiazovivin (SML1045, Sigma Aldrich). 2 μL of each viral construct (TetO-Ngn2-P2A-puromycin ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed three times with 200 μL of PBS-T and developed using 100 μL/well of o-phenylenediamine dihydrochloride (OPD, Sigma P8287) for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Plates were subsequently washed three times with 300 µl/well of PBS containing 0.05% Tween 20 (PBS-T) (Sigma-Aldrich, USA). Non-specific binding sites were blocked using 300 µl/well of 3% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were stored in 24-well plates in PBS (100 mH, pH 7.4 with 0.05% Sodium Azide (Sigma-Aldrich, pH 7.4) for the IHC experiments.
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bioRxiv - Cell Biology 2024Quote: Cells were cultured in 6-well plates with EGM-2 medium supplemented with 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392). Hemodynamic flows were applied to using a 1o tapered stainless steel motorised cone (UMD-17 (Arcus20 Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... Transwells were placed in wells with media containing 10% FBS and 5ug/mL fibronectin (Cat. No. f2006, Sigma-Aldrich, St. Louis, MO). Cells were fixed onto the transwell membrane in 10% formalin with 0.01% Triton X-100 for 10 minutes and stained with DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... transfected HeLa cells were grown overnight on 12-well glass slides at 37°C and treated with 250 nM dexamethasone (Dex) (Sigma-Aldrich, D4902) to induce RGG nuclear import for the indicated times ...
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bioRxiv - Cell Biology 2020Quote: ... bacterial suspension was aspirated and replaced with 500 μl/well of fresh infection media supplemented with gentamicin at 50 μg/mL (Sigma-Aldrich, G1397). This was defined as the time-point T = 0 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... bacterial suspension was aspirated and replaced with 500 μl/well of fresh infection media supplemented with gentamicin at 50 μg/mL (Sigma-Aldrich, G1397) and Erastin at 4 μg/ml as indicated.
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bioRxiv - Cell Biology 2020Quote: Concentration of molecules (OPG,RANKL, Syndecan-1) in the supernatant (200µl) and cell layer (1 well, after washing with PBS buffer (Sigma-Aldrich, #D8537-500ML) and lysis using RIPA buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... the glass coverslips were removed from the wells with the aid of tweezers and added to glass slides with 5 μL of Glycerol (Sigma Aldrich G5516). The primary antibodies used were ...
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bioRxiv - Molecular Biology 2020Quote: ... cells within a well were synchronized by incubating (45 min, 37°C) with 100 μl recording media (per litre: DMEM powder (Sigma cat#D2902), 1.9 g glucose ...
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bioRxiv - Genomics 2020Quote: ... 1-4 PCR wells were combined in a tube and 40 μL of 97% 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma-Aldrich #370533) was added ...
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bioRxiv - Genomics 2019Quote: ... 2×105 HEK293T cells were plated onto each well of a 25 ug/ml poly-D lysine (EMD Millipore, A-003-E) coated 24-well plate ...
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bioRxiv - Neuroscience 2019Quote: ... well-fed L3 worms were mounted on 1% agarose pads with 5 mM sodium azide (Sigma-Aldrich, CAS No. 26628-22-8), whereas dauers were mounted on pads with 9 mM sodium azide (Simpkin and Coles ...
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bioRxiv - Genomics 2021Quote: ... A1413301)-coated 12-well plates at a density in the range of 3-7×105 cells per well in iPS-Brew + 2μM Thiazovivin (Sigma, cat. SML1045-5MG). Alternatively ...
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bioRxiv - Neuroscience 2021Quote: ... iPSC-derived neuron progenitors were expanded using 6-well plates coated with 15 μg/ml Poly-L-Ornithine (Millipore Sigma Cat# P4957) and 10 μg/ml laminin (Invitrogen Cat# 230171015 ...
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bioRxiv - Neuroscience 2020Quote: ... 48-well MEA plates (Axion Biosystems #M768-KAP-48) were coated with 50 µg/mL poly-D-lysine (Sigma-Aldrich #P0899-50MG) in borate buffer ...
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bioRxiv - Cell Biology 2021Quote: Aligned electrospun PDO fibres were stretched and secured over the surface of a 24-well plate Cell Crown™ insert (Sigma-Aldrich). The whole construct was sterilised in 100% ethanol for 60 mins and rinsed five times in sterile 1 x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... The middle region of the cochlea was cultured in a well on a cover glass coated with 1:1 poly-L-ornithine (0.01%; Sigma-Millipore #P4957, USA) and laminin (50 μg/mL ...
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bioRxiv - Microbiology 2021Quote: Cell migration and invasion assays were performed using 24-well transwell chambers with filter membranes 12 µm pore size (Millipore Corporation, PIXP01250). Cells were detached with trypsin-EDTA ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were resuspended and plated on 24-well plates containing cover slips coated with 10 μg/ml poly-D-lysine (Sigma-Aldrich, Germany) at a density of 0.8×105/ml in RPMI medium (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... after 3 days of growth membranes/biofilms were transferred to fresh agar wells containing 20uL of DNase 1 (2 Kunitz units / µL) (Sigma-Aldrich #D4263) spotted directly underneath the biofilm ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were added for 48 hours at 4°C and included the mouse monoclonal antibody described above (31G10) (5) as well as commercially available cell type-specific markers including: choline acetyltransferase (ChAT; AB143; rabbit polyclonal; Millipore, Burlington, MA); COUP TF1-interacting protein 2 (CTIP2 ...
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bioRxiv - Biochemistry 2022Quote: ... cells were detached and re-plated at 60,000 cells per well into a poly-L-ornithine-coated (Sigma-Aldrich; cat. no. P3655) white 96-well plate (Thermo scientific ...
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bioRxiv - Immunology 2022Quote: ... The wells were gently covered with 500 µL of MEM semi-solid medium containing 1.5% CMC (Carboxymethylcellulose Medium viscosity; Sigma Aldrich, cat: C4888), 2% SFB ...
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bioRxiv - Cancer Biology 2022Quote: HEK293T cells at 80-90% confluency in a 12-well plate were transfected using 6 μL GeneJuice Transfection Reagent (Sigma-Aldrich, 70967) and 100 μL RPMI 1640 medium (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... in a 24-well plate were coated with 4 mL per plate or 500 μL per well of 50 μg/ml poly-D-lysine (PDL, Sigma, catalog #: P6407) at 4°C overnight ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... HEK293T cells were seeded in a 6-well plate so that they were 70-90% confluent the next day (∼0.8x10^6 - 1x10^6 cells/well in 2 mL of standard growth media containing 10% FBS (Millipore, TMS-013-B) and 1x Penicillin-Streptomycin (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... or 900-fold (SARS-CoV-1) diluted plasma samples before addition of THP-1 cells (20,000 cells per well; Millipore Sigma, Burlington, MA). After 19 h incubation at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded in 6-well plates the previous day and treated either with 200ug/ml Cycloheximide (CHX) (Sigma-Aldrich # C-7698) in fresh growth media for 2 ...
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bioRxiv - Neuroscience 2019Quote: ... 48-well MEA plates (Axion Biosystems #M768-KAP-48) were coated with 50 µg/mL poly-D-lysine (Sigma-Aldrich #P0899-50MG) in borate buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The non-adherent cardiomyocytes were seeded in complete Cardiomyocyte plating medium supplemented with 10% fetal calf serum and antibiotics at a density of 4-5 × 105 cells/well on Poly-L-Lysine (Sigma P-7890) and Gelatin (Sigma G9891)-coated coverslips in 6-well plates ...
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bioRxiv - Biochemistry 2021Quote: ... incubated for ~3 h at room temperature and finally the wells were thoroughly rinsed with phosphate-buffered saline (PBS; Sigma Aldrich, UK). The coverslips remained functional for at least two weeks when stored at 4°C in plastic pipette tip box containing a layer of deionised water at the bottom ...
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bioRxiv - Bioengineering 2021Quote: ... the hydrogels were removed from the molds and placed into wells containing freshly prepared 1.25 mg/mL aqueous solution of 1,4-dithiothreitol (DTT, Sigma-Aldrich, Saint Louis, MO) on a shaker at RT for 8 min ...
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bioRxiv - Bioengineering 2020Quote: ... the cells migrated through the membrane to the lower layer of each well were counted by H33342 staining (Sigma, St Louise, USA).
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bioRxiv - Bioengineering 2020Quote: ... Purified CD34+ EC progenitors were then seeded onto a 0.2 mm ibidi μ-slides or an ibidi 24-well μ-plate precoated with 10 μg/mL Collagen IV (Sigma Aldrich). 24 hours after the cells were seeded ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Coating solution was then removed by flicking and plates were washed with OptiMEM and blocked with 25 ul/well of assay buffer (1% Ovalbumin (Sigma #A5503-10G) in OptiMEM containing 1X Pen-Strep (Gibco #15140-148)) ...