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Citations for Millipore Sigma :
8401 - 8450 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... NSC-34 or C2C12 cells were plated in 24-well plates containing poly-D lysine (P-7280, Sigma-Aldrich, Saint Louis, MI, USA) coated coverslips and then transfected with wild type or mutant BAG3-GFP constructs as described above for WB and FRA experiments ...
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bioRxiv - Cell Biology 2021Quote: The cells attached to the 48-well plate were fixed for 10 min with 10% buffered formalin Plus (Sigma, Saint Louis, Missouri, USA) and then permeabilized for 15 min with 0.01% Tween-20 (Serva ...
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bioRxiv - Cell Biology 2020Quote: ... The PDMS coated surface of the 50 mm FluoroDish dish as well as the side of the coverslip bearing the PDMS pillars structures were incubated with 100 μg/ml BSA (Sigma-Aldrich, #A9647-100G) in PBS for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.105 Met-5A cells were plated in Ibidi µ-Slide 8 Well (Clinisciences #80826) coated with collagen type I (Sigma-Aldrich #C3867-1VL) and incubated for 48h at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were transferred to 6 well culture dishes and rehydrated with MilliQ water and labeled in H2O for 5 min with Dapi (Sigma-Aldrich, Cat # 9542) 1:10,000 dilution of 1 mg/ml aqueous stock and Syber Gold 1:20,000 dilution of DMSO stock (ThermoFisher Cat # S11494) ...
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bioRxiv - Biochemistry 2022Quote: ... were dissected from E13.5 wild-type mice and plated in a 8-wells chamber coated with 10µg/ml poly-d-lysin (Sigma-Aldrich, St Louis, MO) and 10µg/ml mouse Laminin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were plated in 24-wells coated with a 40 mg/mL solution of Poly-HEMA (Poly 2-hydroxyethyl methacrylate; Sigma Cat. no. P3932) in complete media ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 100 μL of medium in each well was removed from the top and 100 μL of 1% collagenase (Sigma, cat. no. C9263-1G) in FBS free medium was added into the well and incubated at 37 °C for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: 4T1 cells were plated at a density of 1.0 × 105 cells per well in 6-well tissue culture plates and culture medium was replaced after 24hr with medium containing 8µg/mL of polybrene (Sigma-Aldrich, Lismore, NSW, Australia). The cells were infected overnight with the concentrated virus at 1:5 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples from each well were concentrated to ∼30-μL with Amicon Ultra 10-KDa molecular weight cutoff spin filters (EMD Millipore Cat#UFC501008) for 2-hrs before loading into a 4-12% Bis-Tris SDS-PAGE gel (Invitrogen Cat#NP0322BOX) ...
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bioRxiv - Cell Biology 2021Quote: ... The wells were subsequently blocked for 1 hr in blocking solution (25 µL of 1% BSA [Sigma], 0.2% Tween-20 in 1x PBS) then incubated with 25 µL of the Click-iT EdU detection mix (prepared as per manufacturer’s instructions using Alex Fluor 455® ...
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bioRxiv - Cancer Biology 2020Quote: Ovarian cancer cell lines (5 × 104 cells per 24 well) in complete media containing 8 μg/mL Polybrene (Millipore-Sigma: TR-1003-G) were transduced with high-titer Luc-mCherry lentivirus (64) ...
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bioRxiv - Cancer Biology 2020Quote: Ovarian cancer cell lines (5 × 104 cells per 24 well) in complete media containing 8 μg/mL Polybrene (Millipore-Sigma: TR-1003-G) were transduced with high-titer Luc-mCherry lentivirus (64) ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were performed in 6 μL volumes on 384-well plates in reaction buffer containing 3 μL of 2 x Kapa Probe Fast qPCR Master Mix (Sigma-Aldrich, Dorset, UK). All probes were supplied by Thermo Fisher Scientific (Massachusetts ...
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bioRxiv - Cancer Biology 2021Quote: ... larvae were anesthetized in 1x Tricaine and embedded in a 96-well ZF plate (Hashimoto Electronic Industry Co, Japan) with 0.5 % ultra-low gelling agarose (Cat. No. A2576-25G, Sigma-Aldrich Chemie GmbH, Germany). The Operetta CLS high-content imager (PerkinElmer ...
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bioRxiv - Cell Biology 2022Quote: ... All iBAT pieces from one mouse were transferred into a well of a 12-well dish containing 0.5 ml high-glucose DMEM supplemented with 2% FA-free BSA (A6003, Sigma-Aldrich, St. Louis, MO) and 0.4 µCi [14C(U)]-D-glucose (ARC0122 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells (2-5 × 105) were seeded in a six-well plate in the presence of polybrene (10 µg/ml; Sigma-Aldrich; TR-1003G) and 25 µl of lentivirus was added to the well ...
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bioRxiv - Bioengineering 2020Quote: The effect of HMGB1 on inducing the migration of rat BMSCs were examined using a Trans-well plate (Millipo Sigma, St Louise, USA). The rat BMSCs in DMEM at passage 1 were seeded into the upper layer of insert well of the trans-well plate ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Worms were cultured in 6 well dishes (4-5 mature male worms or 8-10 immature worms in 3mL media per well) at varying concentrations of praziquantel (PZQ, Sigma-Aldrich cat. # P4668) or DMSO vehicle control and imaged to record phenotypes.
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bioRxiv - Cancer Biology 2021Quote: The migration of two TNBC cells (MB231, MB468) were performed using a 24-well transwell chambers (8 µm pore size; Millipore, Billerica, MA, USA). The cells were plated in triplicates into the upper chamber (1 × 105 cells/well ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... boric acid and ascorbic acid as well as reagents required for 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) synthesis were obtained from Sigma-Aldrich (Bangalore, India). Acetone was obtained from Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... untagged human α-synuclein (100 μg/well) —purified as previously described [17]—and 10 μM ThT in dPBS (Sigma-Aldrich, St. Louis, MO) were combined in a final volume of 95 μl of dPBS ...
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bioRxiv - Immunology 2020Quote: Approximately 5×104 mononuclear cells/well were plated in duplicates per sample in 100μl of RPMI-1640 culture media (Sigma Aldrich, St. Louis, MO) supplemented with 10 % fetal bovine serum and 1 % Penicillin and Streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... 1ml of the medium/well) were incubated at 37°C for 24 hours with 0.1 nM retinoic acid (Sigma-Aldrich, St. Louis, MO) and 2 ng/ml recombinant mouse TGF-β (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... 100uL per well of culture medium was replaced with 100uL fresh complete media supplemented with 50U/mL IL-2 (Millipore Sigma, Cat # 11147528001) on days 1,3 and 5 unless otherwise stated ...
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bioRxiv - Neuroscience 2021Quote: ... were collected in RIPA buffer supplemented with 1x cOmplete protease inhibitor cocktail as well as phosphatase inhibitor cocktail 2 at 1:100 dilution (#P5726, Sigma-Aldrich, MO, USA). Samples were stored at −70°C until analysis ...
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bioRxiv - Cell Biology 2022Quote: ... or 1 ml (6-well plates) of lysis buffer (Tris 50 mM; NaCl 150 mM; pH 7.3; Triton X-100 1 % (Sigma-Aldrich. Cat. Nº: T9284); Leupeptin 5 µM (Focus Biomolecules ...
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bioRxiv - Cancer Biology 2022Quote: Cells were grown to near confluency on 12-well plates and treated on the following day with 2 μg/mL Doxycycline (Sigma-Aldrich, cat# D9891) or vehicle ...
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bioRxiv - Immunology 2022Quote: ... we washed the wells and treated them for 1 h at RT with 1:30,000 diluted Extravidin-ALP conjugate (Sigma, St. Louis, MI, USA). After washing ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RBD (2.5 µg/mL) was adsorbed overnight at 4°C to a 384-well Nunc MaxiSorp ELISA plate (Millipore Sigma, St. Louis, MO). The RBD was removed via aspiration and the plate blocked with 3% BSA in TBST for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were cultured on a 6-well to confluency and degradation was induced by adding 500 nM dTag-13 (Sigma-Aldrich, SML2601-1MG) or 500 µM auxin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... A third set of duplicate wells was identical to the second but with addition of 10ng/ml interferon-γ (IFN-γ; Sigma-Aldrich) to the media.
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 μg ml-1, Sigma-Aldrich, St. Louis, MO, USA) and cultivated in DMEM medium (PAA Laboratories GmbH ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were seeded at a density of 8 x 106 cells/well in DMEM without antibiotics on 10 cm plates coated with γ-irradiated poly-D-lysine hydrobromide (Sigma Catalog #P7405). Cells were cotransfected using TransIT 2020 (MirusBio Catalog #5404 ...
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bioRxiv - Neuroscience 2024Quote: ... 2 mM Glutamax (gibco 35050-061)) onto prepared 12 or 24 well plates coated with 60 mg/ml Poly D lysine (Millipore A-003-E)) in borate buffer (50mM boric acid ...
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bioRxiv - Neuroscience 2024Quote: ... disaggregated cells of the secondary SVZ neurospheres were cultured in 48 wells pretreated with poly-D-lysine (Sigma-Aldrich, St. Louis, MO, USA) and a differentiation medium (DMEM/F12 supplemented with 1% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... astrocytes were thawed and plated at 20,000 cells/well in MEM with 10% Horse Serum and 6.7% glucose onto 24 well plates (Nest Scientific, 70201) containing 25mm coverslips pre-coated with 1mg/ml of Poly-L-ornithine (Sigma-Aldrich, P4957-50ML) for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... and cultured at density 1.25×106 cells/ml in wells coated with anti-CD3 antibody (0.5 μg/ml; OKT3, Sigma-Aldrich, Saint Luis, Missouri, USA), in RPMI medium (Gibco ...
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bioRxiv - Immunology 2024Quote: ... at a density 1.3x10^5 cells/well and treated with 100 nM bafilomycin A1 (Baf-A1, dissolved in DMSO at 0.16 mM; Sigma Aldrich, Cat. No. SML1661) for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... and either CK2α or a D156A analogue was added to each well (1 μM) in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). Phosphorylation reactions were allowed to proceed for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: One day before plating, 6-well MEA plates (Axion Biosystems, M384-tMEA-6W) were coated with poly-D-lysine (Millipore Sigma P6407-5MG) in borate buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... 100ul of ddH2O was added to each well followed by 25ul of 50mg/ml N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (Sigma, St Louis, Missouri) and 25ul of 50mg/ml N-Hydroxysuccinimide (Sigma ...
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bioRxiv - Microbiology 2023Quote: HeLa cells grown on glass coverslips in 24 well plates were pretreated with DMEM containing 30mg/ml of DEAE-Dextran (Sigma, St Luis, MO) for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... the isolated ganglia were immediately transferred into a 24-well plate containing digestion solution (1 ml high glucose DMEM and 100 μl Collagenase Type IV (Sigma; C1889-50 mg) and incubated at 37°C for 40 min ...
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bioRxiv - Cancer Biology 2023Quote: 4×103 SW756 or HeLa cells were cultured in medium according to (51) in 48-well plates coated with 1% low attached agarose (Sigma Aldrich, Taufkirchen, Germany). For heterospheroids 4×103 SW756 and 2×103 Th17 cells were used ...
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bioRxiv - Microbiology 2023Quote: Highly synchronized ring stage parasite cultures of line MD3-pfama1-GFP-BirA and Percoll-purified immature gametocyte cultures of the MD3-pffnpa-GFP-BirA parasite line as well as WT NF54 as control were treated with biotin (Sigma-Aldrich; Taufkirchen, DE), at a final concentration of 50 µM for 24 h to induce the biotinylation of proximal proteins by the BirA ligase ...
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bioRxiv - Neuroscience 2023Quote: ... 315-319 mOsm) with a ceramic blade and placed directly onto 6-well Millipore Millicell cell culture inserts (Millipore Sigma PICM0RG50, Burlington, Massachusetts) over 1 mL warmed neuronal media (1x MEM (Millipore-Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... The wells were then stained with 1.5 mL of SRB solution 0.057% in 1% acetic acid (ref 341738, Sigma Aldrich, Saint Louis, Missouri, USA) for 30 min at room temperature under gentle agitation ...
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bioRxiv - Microbiology 2023Quote: ... monolayers in 24-well plates at 70% confluency followed by the addition of semisolid media containing 0.8% methylcellulose (Sigma #M0512. Viscosity 4,000 cP) DMEM containing 2% fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 100,000 nuclei were sorted at a ratio of 70% NeuN+ and 30% NeuN-into a single well of a PCR plate (Sigma-Aldrich, EP0030129504-25EA) containing 50 µL FANS buffer (see Table S8) ...