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Citations for Millipore Sigma :
7951 - 8000 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Oocyte live cell imaging was done in 4-well Labtek chambers (ref. 155383) in 4 μl drops of M16 medium covered with mineral oil (Sigma) in a 5% CO2 environmental microscope incubator at 37 °C (Pecon) ...
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bioRxiv - Immunology 2021Quote: ... total mononuclear cells isolated from the epithelium were plated in 48-well plates and incubated at 37° C with 100ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma), 200ng/mL ionomycin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... and the organ was cultured in a well on a cover glass coated with 1:1 poly-L-ornithine (0.01%; Sigma-Millipore, USA) and laminin (50 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... 25.000 NSPC per well were seeded into 12-mm coverslips coated with laminin (L2020, Sigma-Aldrich, St Louis, MO, USA) as previously described (Silvestre et al. ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing dissociated cortical neurons was seeded on 12 or 24 well plates containing coverslips (Neuvitro Corporation) precoated overnight with 50 µg/ml poly-D-lysine (Sigma). Twelve-well plates contained 18 mm coverslips seeded at a density of 200’000 cells per well and 24 well plates contained 12 mm coverslips seeded at a density of 100’000 cells per well ...
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bioRxiv - Neuroscience 2022Quote: ... The coverslips were washed three times with PBS 1X at room temperature and fixed in 1 mL/well of PFA 4% (Sigma) under the chemical hood for 10 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... 40μg of total protein were loaded per well in SDS-acrylamide/bisacrylamide gel and proteins were electrophoretically transferred to a PVDF membrane (Millipore). For treatment with MG132 and ABA ...
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bioRxiv - Cancer Biology 2022Quote: ... and additional wells with 20% biotinylated PLL-g-PEG and FNIII(7-10) were supplemented with 1 µM staurosporine (Merck Millipore) to serve as a positive control for apoptosis ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mM PMSF and 0.25 mg/ml lysozyme as well as a cOmplete™ Protease Inhibitor Cocktail Tablet (Millipore Sigma) for every 50 ml) ...
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bioRxiv - Bioengineering 2022Quote: ... was assembled around the region containing photopatterned ssDNA features and wells were incubated in 10-20 μg ml-1 bovine serum fibronectin (1 mg ml-1, F1141, Sigma) diluted in sterile 50 mM HEPES (pH 8.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.5×105 B16-F10 cells were plated into a 6-well dish and stimulated with growth media supplemented with 10 or 20 μM forskolin (Sigma) and 100 μM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Bioengineering 2022Quote: ... the wells were rinsed 3 times with PBS and covered with 500 μL 2% Alizarin Red solution (Sigma-Aldrich, 05600) diluted in UPW for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 100 mg fragments were incubated in a 12-well plate with M199 media ± 7 nM insulin (Actrapid, Novo Nordisk) and 25 nM dexamethasone (D4902, Sigma). After 24 hr incubation ...
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bioRxiv - Cell Biology 2022Quote: ... to sort each well containing 80 nuclei per well into new four 96-well plates (in total of 384 wells) containing 8 μL primer dilution buffers (15 pmol primer i7, 15 pmol primer i5, 200 ng BSA (Sigma), 0.027% SDS ...
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bioRxiv - Immunology 2022Quote: ACH2 cells with blocked expression of SLFN11 and SLFN12 (as described above) were seeded in a 24-well plate and treated with DMSO (0.01%, Sigma-Aldrich) or SAHA (Vorinostat ...
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bioRxiv - Immunology 2022Quote: ... Progenitors were spinfected in 6-well plates at 23-32°C for 30 minutes at 1000 x g with 4 µg/mL polybrene (Sigma) and 1 µM β-estradiol at 0.25 × 106 cells/mL for single gene knockouts ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 1 hour at 37 °C/5% CO2 and washed three times with 50 µl per well of PBS (Sigma). Immortalised human umbilical vein endothelial cells (Ea.Hy 926 cells ...
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bioRxiv - Genomics 2022Quote: ... The probe mix was then removed and the wells were washed with 100μl of 0.1X SSC (SSC, Sigma-Aldrich, USA). To covalently attach the now hybridized 16S probes onto the surface probes ...
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bioRxiv - Genetics 2022Quote: ... 0.2 × 106 mESCs were seeded per 12-well and transduced the next day with 50 μl of concentrated viral supernatant and 8 ng/μl polybrene (Sigma). Antibiotic selection was started 2 days after transduction and kept until all cells in the non-transduced control were dead (typically within 1-2 passages).
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bioRxiv - Microbiology 2022Quote: ... Duplicate samples of 20 µL of a 2.5 µg/mL solution of a purified RBD-mi3 nanoparticle in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: 20 µL of a 2.5 µg/mL solution of an affinity purified His-tagged RBD in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then fixed for 1 hr with 1mL/well of 10% neutral buffered formalin (NBF) (CellPath) and stained for 1 hr with 0.1% Toluidine blue (Sigma-Aldrich) dissolved in H2O.
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bioRxiv - Microbiology 2022Quote: ex-vivo mouse fecal experiments with sterile and non-sterile mouse fecal media were conducted aerobically at 37°C in 48 well plates (Sigma) with shaking (210 rpms) ...
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bioRxiv - Microbiology 2022Quote: ... The wells were then washed with ice-cold PBST and incubated with HRP-linked goat anti-chicken antibody (Sigma-Aldrich) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were seeded into 12-well tissue culture trays at a density of 2×105 cells per well and pretreated with 10-8 M phorbol 12-myristate 13-acetate (PMA) (Sigma) for 72 h ...
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bioRxiv - Physiology 2022Quote: 24-well tissue culture plates were coated overnight at room temperature with 0.5mg/mL Poly-L-lysine (Sigma Cat# P2636) in 0.5M borate buffer then washed twice with sterile PBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A total of 1 × 105 cells (treated with vehicle or 6.4 µM THZ-P1-2 for 24h) were seeded per well in poly-L-lysine (Sigma-Aldrich) coated Seahorse XF96 plates in 200 μL XF Assay Medium (Modified DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... using 0.5 mM EDTA and either re-seeded with split ratios (1:3 to 1:8) onto 6-well plates or cryopreserved using CryoStor CS10 Cryopreservation Reagent (Sigma). iPSC lines were maintained in 5% CO2 ...
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bioRxiv - Synthetic Biology 2023Quote: HEK293T-LP cells sourced from Matreyek et al38 were plated into a 48 well plate according to the plate reader assay and treated with 0 to 2 ng/μl dox (Sigma) to induce BFP expression ...
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bioRxiv - Neuroscience 2022Quote: ... slices were kept at room temperature in wells containing 0.3% Triton X-100 in PBS and 4% normal goat serum (Sigma-Aldrich) for 3 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated at a density of 30,000 cells/well on glass coverslips (12mm diameter, Carolina Biological Supply 633029) treated with poly-D-lysine (Sigma P6407) and laminin (R&D 340001001 ...
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bioRxiv - Physiology 2022Quote: ... was loaded into wells in triplicate followed by 250 μL of Bradford reagent (B6916, Sigma Aldrich, St. Louis, MI, USA). After incubating for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Nunc six-well plates were treated with poly-D-lysine hydrobromide (1 mL, 100 µg/mL by Sigma Aldrich, P6407) and incubated (37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3×105 cells were seeded into 12-well plates that had been coated with 1 mg/mL poly-L-ornithine (Sigma) for 60 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were cut and placed in a 24-well plate and permeabilised in 0.2% Triton-X100 (Sigma-Aldrich, United Kingdom) in PBS for 20 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Parasitemia was adjusted to 0.2-0.5% and 2% hematocrit and the culture was distributed in triplicates in 6-wells plate with various treatments: 400pM of the mimic (HMI0513-5NMOL, Sigma) or 400pM of the inhibitor (cat# 4464084 ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 1 x 106 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 μg per ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Cell Biology 2022Quote: ... At least one well on each plate served as a negative control by addition of 25μM cytochalasin B (Sigma Aldrich) to block all transporter-mediated glucose uptake ...
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bioRxiv - Developmental Biology 2022Quote: ... 12-well plates at an approximate cell density of 40,000 cells/cm2 in cSFDM with 2 μM Retinoic acid (Sigma, #R2625). From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... was added to the media and cells were harvested at indicated times by washing quickly with 2 ml PBS per well and adding TriPure reagent (Sigma). Cells at time 0 did not receive ActD ...
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bioRxiv - Cancer Biology 2024Quote: ... U2OS cells were transfected 12–24 h after seeding into eight-well Imaging Chamber CG (Zell-Kontakt) with XtremeGENE HP (Sigma) according to the manufacturer’s instructions and incubated for 48 h prior to imaging.
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bioRxiv - Bioengineering 2024Quote: ... THP-1 were seeded into a 6 well plate at 2 x 105 cells/well and treated with 20 ng/mL phorbol-12-myristate 13-acetate (PMA, Sigma) in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Cell Biology 2024Quote: ... each RPE flat mount was individually transferred into a well created by attaching a silicone gasket (Sigma Aldrich #GBL665104-25EA) to a SuperFrost Plus microscope glass slide (Fisher Scientific #12-550-15) ...
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bioRxiv - Molecular Biology 2024Quote: ... were coated with 100 ng/well of each venom diluted in carbonate-bicarbonate buffer (50 mM, pH 9.6, Sigma, C3041) and incubated for 1 hour at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed and incubated with 100µL per well of substrate containing 3,3′,5,5′-Tetramethylbenzidine dihydrochloride (Cat # T3405, Sigma-Aldrich, MO, USA) and hydrogen peroxide for 10 minutes ...
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bioRxiv - Genomics 2024Quote: ... Embryos were then incubated overnight at 37°C under 5% CO2 in humidified air in 4-well culture dishes containing KSOM media (Millipore). Experiments were approved by the SIMR IACUC and were performed following the committees’ guiding principles.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... THP-1 monocytes were seeded (20,000 cells/well) in medium containing 25 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 48 h and afterwards rested for 24 h ...
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bioRxiv - Immunology 2024Quote: Affinity purified His-tagged RBD produced by Expi293 transient transfection was diluted in 0.1 M NaHCO3 pH 9.8 (20 µL of a 2.5 µg/mL solution) and coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...