Labshake search
Citations for Millipore Sigma :
8101 - 8150 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... An aliquot of 30 μL from each sample was subjected to electrophoresis on 8% or 15% polyacrylamide gel for ARAP1 and ADAP1 each and transferred into an Immobilon®-P transfer membrane (Merck Millipore Ltd., Germany). Membranes were blocked with 5% skim milk (Nacalai Tesque ...
-
bioRxiv - Cancer Biology 2024Quote: ... A housekeeping protein was also detected using the same blot by mild stripping and re-probing the membrane with an anti-GAPDH rabbit polyclonal antibody (Sigma-Aldrich, G9545-200).
-
bioRxiv - Neuroscience 2023Quote: ... The plates were centrifuged for 7 minutes at 2000rpm for higher transfection efficiency and were incubated for 1-3 days with coving by Breathe-Easy sealing membrane only (Sigma-Aldrich, # Z380059-1PAK). All assays were performed on Day 1-3 following transfection ...
-
bioRxiv - Biophysics 2022Quote: ... Proteins were transferred to PVDF membranes and subjected to immunoblotting with anti-HIV Ig polyclonal serum (NIH ARRRP) and anti-FLAG monoclonal antibody (Sigma-Aldrich, Clone M2). For immunoblotting of MLV particles ...
-
bioRxiv - Biophysics 2023Quote: ... Stretching is performed using 2x2 cm flexible polydimethylsiloxane (PDMS) membranes (Sylgard184; crosslinker-to-base ratios of 1:14; cured for 24 h at 65°C, Sigma-Aldrich, St. Louis) as described in (45) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... to remove large particles and the filtrates were then filtered through 0.22 μm pore-size membrane filters (Pellicon cartridge, Millipore Corp., Billerica, MA, USA) to collect microbial cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated overnight with primary antibody (rabbit anti-REST1, Millipore CS200555, 1:1000, or mouse anti-GAPDH, Millipore MAB374, 1:500) in TBST with 5% bovine serum albumin (BSA ...
-
bioRxiv - Physiology 2023Quote: ... Equal amounts of protein extracts were subjected to electrophoresis in 8–12% SDS- polyacrylamide gels and then transferred to PVDF membranes (EMD Millipore, Burlintgon, MA). The membranes were blocked with 5% non-fat milk at room temperature and then incubated with relevant primary antibodies at 4°C overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... The same procedure was then applied to the same membranes with anti-tubulin antibodies (mouse anti-tubulin, 1:40000, Sigma Aldrich cat. # T16199). The molecular weight ladder corresponds to Invitrogen SeeBlue™ Plus2 (reference LC5925).
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were then washed with PBST for 5 min and incubated with the following primary antibodies: GAPDH (EMD Millipore, #MAB 374, 1:2000), β-catenin (EMD Millipore ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins in 65 μl of conditioned media and cell lysates per lane were separated on 6% polyacrylamide gels using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred on a poly-vinylidene difluoride (PVDF) membrane (Immobilon-FL, Merck Millipore Ltd., Burlington, MA) using the MiniProteas Blotting Module (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
-
bioRxiv - Cancer Biology 2023Quote: ... were separated by PAGE and transferred to nitrocellulose membranes that were subsequently probed with antibodies against total histone H3 (cat# 05-499, Millipore-Sigma, Burlington, MA), pan-Acetyl histone H3 (cat# 06-599 ...
-
bioRxiv - Cell Biology 2022Quote: Treated cell and tissue lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) for separation and were then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA), which were incubated with the appropriate primary antibodies (all diluted 1:1000 in 1.5% bovine serum albumin (BSA) ...
-
bioRxiv - Cell Biology 2023Quote: ... Gels were run in 1x running buffer and then the proteins were transferred from the gel in ice cold 1x Buffer onto Immobilon®-P Transfer membranes (Merck Millipore, Cat.No. IPVH00005). Transfer membranes were air dried to dehydrate and stored until use ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein samples (were separated by electrophoresis on a polyacrylamide gradient gel, and subsequently transferred to a PVDF membrane (Immobilon FL, EMD Millipore, Billerica, MA). To prevent non-specific binding ...
-
bioRxiv - Neuroscience 2023Quote: ... were subjected to 5% or 13% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (PVDF, Millipore, Bedford, MA, USA). The following primary antibodies were used in this study ...
-
bioRxiv - Physiology 2023Quote: ... Endotoxemia was induced by intraperitoneal (i.p.) injection of purified LPS extracted from the membrane of Escherichia coli 0111:B4 (Sigma-Aldrich, Saint Louis, USA) at a dose of 30 mg/kg as described previously (Zhao et al. ...
-
bioRxiv - Cell Biology 2023Quote: The gels images were acquired using enhanced chemiluminescence (ECL) by treating the membranes in plastic wrap with Immobilon Forte Western HRP Substrate (Millipore. Cat. No.: WBLUF0100) and imaging on Amersham Imager 600 or iBright imaging system ...
-
bioRxiv - Cell Biology 2023Quote: ... was electroblotted (25 V and 1 mA for 20 min) under semi-dry conditions onto a positively charged Hybond®-N+ hybridization membrane (Millipore Sigma, GERPN203B) using a Trans-Blot® TurboTM Transfer System (Biorad ...
-
bioRxiv - Microbiology 2023Quote: ... and concentrated 20-fold using Amicon Ultra Centrifugal Filter Units with a 10-kDa molecular mass cutoff membrane (Millipore Sigma, Burlington, MA, USA) to obtain the ALF physiological concentration within the lung (1 mg/mL of phospholipid) ...
-
bioRxiv - Neuroscience 2024Quote: ... the membrane was washed with TBST buffer and the membrane was set for 2 hours at room temperature with the secondary goat anti-rabbit IgG HRP antibodies (Sigma Aldrich; 1:10000) diluted in a TBST buffer with 3% PVP ...
-
bioRxiv - Microbiology 2024Quote: ... The buffer for the M68N100 mixture was exchanged by ultrafiltration through a 3,500 MWCO membrane (Millipore; 4,500 x g, 30 min, 4 °C) to 20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2024Quote: Jurkat T cells suspended in culture medium were transferred into Costar® Transwell® polycarbonate membrane inserts with 5 μm pore size (Millipore Sigma). Each insert was fitted into
-
bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated by SDS-PAGE at 165V for 70-80 min and then electrophoretically transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA) at 20 V for 1 hour ...
-
bioRxiv - Microbiology 2024Quote: ... The membranes were blocked and then incubated overnight at 4°C with the primary antibodies: Tubulin (Sigma, Cat.#T6074; Cell signaling, Cat.#2144), HA-tag (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2024Quote: ... Retinal mounted membranes were then transferred to 24 well plates (Corning® Costar® TC-Treated Multiple Well Plates, Catalog Number: CLS3524, Millipore Sigma) filled with 1 mL of retinal explant media (50% DMEM ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the following primary antibodies diluted in 5% TBST: GluA1-C (Millipore, Cat# AB1504, 1:1000), GluA2-C (NeuroMab ...
-
bioRxiv - Physiology 2024Quote: ... Follicular membranes were removed from isolated oocytes by collagenase treatment (2 mg ml−1; type 1; Sigma-Aldrich or Collagenase-P from Roche) for 3 hr ...
-
bioRxiv - Plant Biology 2024Quote: ... The FPLC fraction containing NCR13 was concentrated and desalted using an Amicon Stirred Cell with a 3-kD cutoff membrane (Millipore, Cat No: PLBC04310) and further purified by reverse phase C18-HPLC ...
-
bioRxiv - Biochemistry 2024Quote: ... Membrane was blocked with 1% milk solution and probed with Anti-6 x His Primary mouse antibody (Millipore #05-949, 1:1000 dilution) overnight at +4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein were subjected to 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore, Bedford, MA, USA) activated with 100% methanol ...
-
bioRxiv - Biochemistry 2024Quote: ... Pooled fractions were concentrated to 20 mg mL-1 using a 10-kDa cut off filtration membrane (Cat. UFC501096, Merck Millipore, Burlington, MA, USA). Sitting drop crystallization trials were set up by depositing 150 nL of the precipitant solution on top of 150 nL of protein with 70 μL of the reservoir using a Crystal Gryphon LCP robot (Art Robbins Instruments) ...
-
bioRxiv - Biochemistry 2024Quote: ... Equal amounts of protein were subjected to 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore, Bedford, MA, USA) activated with 100% methanol ...
-
bioRxiv - Cancer Biology 2024Quote: ... added with 50 mM dithiothreitol (DTT) and proteins were separated on 13% polyacrylamide gel and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA, USA) for immunoblot analysis ...
-
bioRxiv - Developmental Biology 2024Quote: ... The proteins were separated on a 4–12% SDS-polyacrylamide gel and transferred to a PVDF membrane (Immobilon FL, EMD Millipore, Billerica, MA). The membrane was blocked using Immobilon (Immobilon® Signal Enhancer ...
-
bioRxiv - Developmental Biology 2021Quote: TUNEL analysis was performed using the In Situ Cell Death Detection Kit (Kit #11684795910, Sigma Aldrich) following manufacturer’s recommendations and fluorescein-dUTP ...
-
bioRxiv - Cancer Biology 2020Quote: ... instructions provided by the kit (Annexin V-FITC Apoptosis Detection Kit, Sigma, Catalog No. APOAF-50TST) was followed ...
-
bioRxiv - Microbiology 2022Quote: LDH was measured using an LDH Cytotoxicity Kit (Pierce, 88953) or Cytotoxicity Detection Kit (Sigma, 11644793001) per the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: The Apop-Tag Fluorescein Kit (Millipore) was used for TUNEL staining according to manufacturer’s instruction with minor modifications ...
-
bioRxiv - Biophysics 2021Quote: The Citrate Synthase Kit (Sigma-Aldrich) was adapted for use in this assay (Chakrabortee et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... Chemiluminescence was generated by ImmunoStar detection kit (Wako) or Immobilon (Millipore) and detected by LAS-3000 mini system (FUJIFILM).
-
bioRxiv - Neuroscience 2020Quote: ... and the Immobilon Western kit (Millipore). The chemiluminescence signals were detected by Luminograph II (ATTO ...
-
bioRxiv - Cell Biology 2022Quote: Duolink PLA Kit (Sigma-Alrich, 92101) was used based on the manufacturer’s procedure ...
-
bioRxiv - Developmental Biology 2022Quote: ... and TRAP staining kit (Sigma, USA), respectively ...
-
bioRxiv - Cell Biology 2022Quote: ... Leukocyte (TRAP) Kit (Sigma-Aldrich, 387A) with fast green counterstaining.
-
bioRxiv - Genetics 2022Quote: ... the Immobilion® chemiluminescence kit (Millipore) was used according to the supplier’s recommendations.
-
bioRxiv - Cell Biology 2022Quote: ... coli using plasmid preparation kits (Sigma) and sequence verified by Sanger sequencing (QuintaraBio).
-
bioRxiv - Neuroscience 2022Quote: ... The ApopTag TUNEL stain kit (Millipore) was used for Figures 3B ...
-
bioRxiv - Bioengineering 2022Quote: ... Standard ATP/ADP kits (Sigma-Aldrich) containing D-luciferin ...