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Citations for Millipore Sigma :
7901 - 7950 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... in Spectra/PorTM 1 RC Dialysis Membrane Tubing (SpectrumTM 132650) and concentrated in Amicon ®Ultra-2 mL centrifugal filters (Millipore UFC205024).
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of the extracted protein were electrophoresed in a 15% SDS polyacrylamide gel for 1 hour at 150V and transferred to a PVDF membrane (Millipore, MA, USA) for 1 hour at 72V ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell-free supernatants were centrifuged at 4,000 rpm for 10 min and subjected to filtration with a 0.22-μm polyethersulfone membrane (#SLGPR33RS, Sigma, St. Louis, MO, USA). The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor ...
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bioRxiv - Cancer Biology 2022Quote: ... sEV fractions were collected by a size-based EV isolation method using 50-nm porous membranes (Whatman, WHA110603) with holders (EMD Millipore, SX0002500) by applying a vacuum pressure ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were then blocked with a Tris-Buffered Saline (TBS) solution supplemented with 0.1 % vol / vol polysorbate 20 (Sigma-Aldrich. Cat. Nº:P1379) (TBST ...
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bioRxiv - Microbiology 2022Quote: ... Assembled plasmid was dialyzed on a Type-VS Millipore membrane (MF type, VS filter, mean pore size = 0.025 μm, Millipore, Inc. #VSWP 02500) and electroporated into Endura electrocompetent cells ...
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bioRxiv - Neuroscience 2022Quote: ... Then membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase conjugated secondary antibodies (1:3000, Sigma Aldrich, St. Louis, MO) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... Permeabilization of the membrane and blocking of unspecific binding sites were performed in one step by adding 0.5% Triton X-100 (Sigma-Aldrich, Buchs, Switzerland) to the blocking solution (PBS - Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... skim milk/TBS and the membrane was washed 5x with 1x TBS before incubation with streptavidin-conjugated alkaline phosphatase (dilution 1:1,000, Sigma-Aldrich; Taufkirchen, DE) in 5% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3x with 1x PBS and incubated with appropriate primary antibody (Table 4) diluted in 4% BSA (Sigma, A9418-50G) in 1x PBS overnight at 4C ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting ROS membranes pellet was resuspended in 90 mL ConA buffer containing one tablet of protease inhibitor and the membranes were solubilized at room temperature with lauryldimethylamine-oxide (LDAO, Sigma-Aldrich, USA) to a final concentration of 1 % ...
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bioRxiv - Biophysics 2023Quote: ... We washed the membrane 3 times with TBST before incubating with a 1:1 ratio of horseradish peroxidase substrate and enzyme mixture (Millipore, Burlington, Massachusetts). We imaged using a Biorad Chemidoc camera set to detect chemiluminescence ...
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bioRxiv - Neuroscience 2023Quote: ... 15 µg of sample was mixed with 1 % NativePAGE™ G-250 sample additive followed by electrophoresis on NativePAGE™ Novex® 4-16% Bis-Tris Gels in 1X NativePAGE™ Running Buffer then transfer using 1X NuPAGE® Transfer buffer and 0.45 µm PVDF membranes (Millipore, IPVH00010) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301 ...
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bioRxiv - Cancer Biology 2023Quote: ... were separated by PAGE and transferred to nitrocellulose membranes that were subsequently probed with antibodies against total histone H3 (cat# 05-499, Millipore-Sigma, Burlington, MA), pan-Acetyl histone H3 (cat# 06-599 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation (1 hr, 20°C) in TBST containing skimmed milk or bovine serum albumin (5%, Sigma, Cat# A9418). TBST (Tris-buffered saline with Tween ...
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bioRxiv - Developmental Biology 2023Quote: ... were dissected from distal regions of the lung and placed on Nucleopore Track-Etched Membrane disks (13 mm, 8 µm pore, poly- carbonate) (Sigma, Cat#WHA110414) floating on top of 500 µl of human lung ALI explant media (Advanced DMEM/F-12 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to a 0.45 µm polyvinylidene difluoride (PVDF) membrane (Millipore, MA, USA, #IPVH00010). The membranes were blocked with 3% of bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2024Quote: The total proteins from the ovaries were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Millipore, Bedford, MA). The membrane was blocked in TBST buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... lysing the host cells via syringing with 20 and 25-gauge needles and filtering through a 3 μm Isopore™ membrane filter (Millipore; # TSTP02500). 3.5×105 parasites from each sample were then added into a T175 flask containing confluent HFFs in D10 medium ...
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bioRxiv - Cell Biology 2024Quote: ... Viral supernatant was passed through a 0.45 µm PVDF membrane filter and cells were transduced for 48 h with 10 µg/mL polybrene (Sigma #TR-1003-G).
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bioRxiv - Systems Biology 2024Quote: ... transferred onto nitrocellulose membrane (Amersham #10600041) in transfer buffer (8 mM Tris, 30 mM Glycine, 20 % Methanol) and stained with Ponceau S (Sigma-Aldrich, #P7170). Membranes were blocked in TBS-T + 5% BSA for 1 hr at RT and incubated overnight at 4°C in primary antibodies prepared in TBS-T + 5% BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µL of protein extract were loaded on a denaturing polyacrylamide gel before being transferred to a nylon membrane and probed with an anti-flag M2 antibody (Sigma Aldrich F1804). An anti-β-actin antibody (Sigma Aldrich A2066 ...
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bioRxiv - Physiology 2024Quote: ... 20 µg total protein extracts were separated by 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and cut into 2-3 parts ...
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bioRxiv - Microbiology 2024Quote: Immunoblotting assays were realized in PVDF membranes using Strep-Tag® II monoclonal antibodies (at 1:1000 dilution from a 200 μg/ml solution) (Novagen®) and immunoglobulin G-alkaline phosphatase conjugates (at 1:5000 dilution) ...
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bioRxiv - Microbiology 2024Quote: ... and propidium iodide (PI, indicator for compromised cell membranes and cell walls; Ref. P4170; Lot. WXBD4453V; Merck KGaA - Sigma-Aldrich, Darmstadt, Germany) was applied to live cells ...
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bioRxiv - Immunology 2024Quote: ... Samples (20 μg of protein per lane) were resolved on 8%-15% SDS-PAGE gels and transferred to polyvinylidene difluoride membranes (Sigma Cat#IPVH00010) using a wet transfer system ...
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bioRxiv - Microbiology 2024Quote: ... The transferred proteins on the membrane were incubated with blocking solution [5% (w/v) bovine albumin serum (BSAV-RO, Sigma-Aldrich, USA) in 1X PBS] for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Samples (20 μg of protein per lane) were resolved on 8%-15% SDS-PAGE gels and transferred to polyvinylidene difluoride membranes (Sigma Cat#IPVH00010) using a wet transfer system ...
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bioRxiv - Bioengineering 2024Quote: ... were grown overnight in 100 µL LB (supplemented with 34 µg/mL chloramphenicol and 50 µM IPTG) in a 96-well plate sealed with Breathe-Easy ® sealing membrane (Z380059, Sigma-Aldrich) at 30 °C with 1100 rpm on a table top shaker (Thermomixer comfort ...
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bioRxiv - Cell Biology 2024Quote: ... filtered through a 0.45 μm membrane and diluted 1:1 with complete medium containing 10 ug/ml polybrene (Millipore, TR-1003-G). Target cells (hTERT-RPE1 Plk1flox/Δ ...
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bioRxiv - Immunology 2024Quote: ... the nitrocellulose membranes containing the purified recombinant fusion proteins were incubated with mouse anti-His antibody [clone HIS.H8] (Millipore Sigma, Burlington, Massachusetts). HRP-conjugated rabbit anti-mouse IgG antibody (SouthernBiotech ...
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bioRxiv - Microbiology 2024Quote: ... Samples were run on an SDS-PAGE gel before being transferred to Immobilon-FL PVDF membrane paper (Millipore Sigma, 0.45 um, #IPFL00010). Blots were blocked with 5% milk in TBS-T (Tris-buffered saline with 0.1% Tween ...
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bioRxiv - Molecular Biology 2024Quote: ... was used for blot imaging on the high sensitivity setting with incremental image capture after the membrane had been treated with chemiluminescent HRP substrate (Millipore Sigma #WBLUF0100). ECL signal capture times displayed varied with target from ∼1–5min and were free of pixel saturation in any signal displayed in the figures ...
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bioRxiv - Molecular Biology 2024Quote: ... were separated by 8-10% SDS-PAGE under reducing conditions and subsequently transferred onto Immobilon-FL PVDF membranes (Millipore, cat no# IPFL00010). The membranes were blocked with 3% skimmed milk in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were decorated with primary antibody (rat anti-CD9, eBioscience, 14-0091, 1:1000; rabbit anti-calnexin, Sigma-Aldrich, C4731, 1:3000) diluted in 5% milk/TBS-T at 4°C over night ...
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bioRxiv - Plant Biology 2024Quote: ... The seedlings were cut perpendicularly in the upper hypocotyl with an injection needle tip (NN-2613S; TERUMO, Tokyo, Japan) on a membrane filter (HAWP04700; Merck Millipore, Darmstadt, Germany). The obtained scion was connected with the partner rootstock through a 0.4-mm silicon microtube (1-8194-04 ...
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bioRxiv - Synthetic Biology 2024Quote: ... A thin film of excised bronchial tissue from rats was evenly distributed in the space between a pair of semi-penetrating membrane filters (Merck Millipore, 2.0 μm) placed between the donor and acceptor chambers ...
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bioRxiv - Cancer Biology 2024Quote: ... Western blotting was performed (refer to “Western Blotting” section for more information) and membranes were incubated in anti-puromycin antibody (Millipore, Cat#MABE343). Quantification of bands was performed by ImageJ.
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bioRxiv - Bioengineering 2024Quote: ... PAMAM dendrons were then purified by size exclusion chromatography on Sephadex G25 followed by membrane ultrafiltration (MWCO 10 kDa, Millipore, Billerica, MA). Streptavidin (SAV ...
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bioRxiv - Bioengineering 2024Quote: ... SAV-PAMAM G4 dendron conjugates were purified by ultrafiltration across a molecular weight cut-off membrane of 50 kDa (Millipore, Billerica, MA). SAV-conjugated G4 was linked to biotinylated recombinant APP2-Fab as described previously30 ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of proteins per sample were subjected to SDS-PAGE and transferred to Immobilon-PSQ PVDF transfer membrane (Merck Millipore, Darmstadt, Germany) with Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... The hydrophilicity of the PDMS base membrane was adjusted through treatment with a 50 mg/mL aqueous solution of tannic acid (Sigma- Aldrich, 403040) in deionized water ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were then blocked in 5% milk for 1Lh and immunoblotting was performed using antibodies to Vinculin (1:1000; Millipore, Catalog# CP74), Tom20 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei extraction was performing using the Nuclei Isolation Kit: Nuclei PURE Prep Kit (Millipore Sigma) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Plant Biology 2020Quote: ... and purified with a commercial kit according to manufacturer’s instructions (His-bind buffer kit, Millipore).
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bioRxiv - Neuroscience 2024Quote: ... Commercially available kits were used for determination of triglycerides (Serum triglyceride determination kit; TR0100; Sigma), non-esterified fatty acids (NEFA-HR(2) ...
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bioRxiv - Plant Biology 2020Quote: ... water was removed and replaced by 100 µl of the assay solution: 17 µg/mL Luminal (Sigma-A8511, USA), 10 µg/ml Horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2020Quote: ... The GTPase assay (20 ul in 25mM HEPES pH7.5, 15mM KCl, 2mM MgCl2) was performed in presence of 1mM GTP (Sigma) using 1μM protein for 0-30 min at 37°C ...