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Citations for Millipore Sigma :
8001 - 8050 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Proximity Ligation Assay (PLA) was performed using the Duolink® PLA reagents according to the manufacturer’s instructions (Sigma Aldrich). An EVOS M7000 imaging system (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: To assess localisation immunofluorescence assays were performed as described previously (31) using anti-HA (1:500, anti-rat,Sigma), anti-MYS (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... cell lysates were tumbled for one hour at 4°C with 20uL of RAS assay reagent (Millipore; #14-278).
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bioRxiv - Cell Biology 2023Quote: ... All following steps of the proximity ligation assay were performed according to suppliers’ instructions (Duolink® PLA; Sigma-Aldrich). Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436 ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (60) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.1.1.27) activities and concentrations of pyruvate and lactate were measured in samples via enzymatic assays (all from Sigma-Aldrich). All assays have been optimized for the current sample types as explained in the previous studies [37,48] and specific protocol settings for each assay can be found in [37] ...
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bioRxiv - Physiology 2023Quote: ... Triacylglycerol concentrations were determined using a spectrophotometric assay with a 4:1 Free Glycerol Agent / Triacylglycerol Agent solution (Sigma Triglyceride and Free-Glycerol reagents ...
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bioRxiv - Neuroscience 2023Quote: Protein lysates were collected from primary human and murine spinal cord astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Experimental evolution and all root colonization assays were performed in half strength Murashige and Skoog (MS) basal medium (2.22 g L−1, Sigma) supplemented with 0.05% glycerol ...
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bioRxiv - Immunology 2023Quote: Mouse sera were diluted 1:100 in an assay buffer of 0.05% PBS-T supplemented with 3% (w/v) BSA (Sigma) and transferred into a 96-well plate ...
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bioRxiv - Microbiology 2023Quote: Cellular cytotoxicity was measured by a colorimetric assay that makes use of thiazolyl blue tetrazolium bromide (MTT: Sigma, USA). Sub-confluent monolayer culture of RAW264.7 ...
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bioRxiv - Molecular Biology 2023Quote: ... The plate was incubated for 4 h at room temperature and the assay then harvested onto a GF/C filter plate (PE) that had been presoaked in 0.3% polyethyleneimine (Sigma) using a Packard Filter Mate Harvester ...
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bioRxiv - Microbiology 2023Quote: The effect of drug treatments on cell viability was assessed using the MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (the MTT powder was obtained from Sigma Aldrich). VTN or Huh-7 cells were seeded the day before treatment at a concentration of 7L×L103 cells per well in DMEM supplemented with 10% FBS and 1% Pen/Strep ...
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bioRxiv - Bioengineering 2022Quote: ... Another part of the solution was used to perform a GAG assay with 1,9-dimethylmethylene blue (DMMB) (Sigma-Aldrich) dye ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were then spun at 21,000 g for 15 min at 4°C and protein concentration determined using Bradford assay (Sigma). Approximately 3-4mg of total cell extract was mixed with 20μl of M2-anti-FLAG agarose beads (bed volume ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (Pizzato Scomazzon et al. ...
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bioRxiv - Cancer Biology 2023Quote: T98G cells treated with DMSO (vehicle) or CHIR99021 (8.6 μM) for 24 h were harvested in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor (Sigma). Proteins were separated by 12% SDS-PAGE according to [29] ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 200 µL assay buffer supplemented with 0.6 mg∕mL 3× Flag® peptide (Sigma Aldrich), and analysed by Western blotting.
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bioRxiv - Cancer Biology 2023Quote: ... Raw ECAR and OCR measurements taken by the instrument from individual wells were normalised using crystal violet staining of plates immediately upon completion of the assay as follows: cells were fixed in 4% PFA and stained using 0.5% crystal violet (Sigma) before solubilisation in 2% SDS (Severn Biotech ...
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bioRxiv - Developmental Biology 2024Quote: Proximity ligation assay (PLA) was carried out with Duolink® In Situ Detection Reagents Far Red (Sigma Aldrich, #DUO92013) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The intracellular ATP concentration in each sample was determined after recovery by using a luciferase assay according to the manufacturer’s instructions (Sigma). ATP concentrations were estimated by using a calibration curve (ATP disodium salt ...
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bioRxiv - Immunology 2024Quote: ... D562 cells were maintained in assay medium (Dulbecco’s Modified Eagle Medium with 10% fetal bovine serum, and 1x Pen-Strep (Sigma)) ...
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bioRxiv - Bioengineering 2024Quote: Cell metabolic activity in dense collagen gels was analysed by alamarBlue® assay (Sigma-Aldrich, St. Louis, MO, USA) very week up to day 21 of culture ...
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bioRxiv - Biochemistry 2024Quote: ... The positive control for the glucosylceramide N-deacylase assay was recombinant Sphingolipid ceramide N-deacylase (SCDase; Sigma-Aldrich, S2563).
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bioRxiv - Biochemistry 2024Quote: ... The positive control for the glycolipid N-deacylase assay was recombinant Sphingolipid ceramide N-deacylase (SCDase; Sigma-Aldrich, S2563) (76) ...
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bioRxiv - Immunology 2024Quote: ... and pellets were resuspended in 75 µL radioimmunoprecipitation assay (RIPA) buffer supplemented with 100x protease inhibitor cocktail (Sigma-Aldrich) and lysed through sonication ...
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bioRxiv - Pathology 2024Quote: ... The final volume in this assay was 100 μl in 1× mitochondrial storage buffer (MITOISO2®, Sigma-Aldrich, Germany). Supernatant fractions were obtained by pelleting the mitochondria (16,000 ×g ...
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bioRxiv - Pathology 2024Quote: ... Successful anemia induction was verified by a quantitative colorimetric assay for determining hemoglobin concentration in whole blood using Drabkin’s reagent (Sigma). Iron deficiency was chosen as our primary anemia model as it is the underlying driver for common causes of chronic anemia (e.g. ...
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bioRxiv - Immunology 2024Quote: ... ELISAs were performed as instructed by the manufacturing procedure (Insulin: Invitrogen; Cortisol: Arbor Assays; LDH: Sigma-Aldrich, PF4: Invitrogen). Plates were read by a BioTek Synergy H1 Plate reader (Agilent).
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bioRxiv - Cell Biology 2024Quote: ... 4°C for 5 minutes before being resuspended in ALDEFLUOR™ assay buffer containing 0.2 μM DAPI (Sigma-Aldrich). Cells were sorted using a FACS-ARIA II (BD Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized with 0.3% Triton-X-100 in PBS for 10 min at RT and then the proximity ligation assay protocol was performed according to the manufacturer’s instructions (Duolink, Sigma). The following primary antibodies were used ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluates were resolved on a 10% SDS-PAGE gel and the proteins were electroblotted onto PVDF membranes (Millipore, Immobilon-P, Cat # IPVH20200) for probing with following primary and secondary antibodies ...
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bioRxiv - Biophysics 2021Quote: ... sonicated for 25 min in a cold water bath and filtered first through a 0.22 µm and then through a 30 kD centrifugal membrane filter (Millipore Sigma, UFC30GV and UFC5030).
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bioRxiv - Cancer Biology 2021Quote: ... Twenty micrograms of total protein were separated on 10% SDS- PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore Co., Bedford, MA). Protein transfer was checked by staining the membrane with Ponceau S red (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... Approximately 20–30 μg total lysates of cell lines or IP-eluted samples were separated by 10% or 12% SDS-PAGE gels and subsequently transferred to PVDF membranes (Millipore, Billerica, MA, USA). Membranes were blocked in 5% skim milk in TBST (0.05% Tween20 ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein was separated by 12% sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) electrophoresis and then was transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA), which was followed by incubation with antibodies against STAP1 ...
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bioRxiv - Molecular Biology 2020Quote: ... were separated by 8% and 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) before transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% skim milk compound with TBST for 3h and incubated with corresponding primary antibodies and appropriate secondary antibodies before the protein bands were detected with ECL-chemiluminescent kit (ECLplus ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were blocked for 1 h in Tris-buffered saline (TBS) with 5% milk and 0.05% Tween 20 (Sigma-Aldrich, St. Louis, MO) and washed 3 times (5 min each ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The flow-through was concentrated using an Amicon 15mL-volume centrifugal concentrator unit equipped with a 3kDa MWCO membrane (EMD-Millipore, Burlington, MA, USA) before being injected onto a Superdex 75 16/600 HiLoad column (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (30 μg) from the cell lysate were resolved by SDS–PAGE and transferred to Immobilon-P membranes (Millipore Corp., Billerica, MA, USA). Membranes were incubated with anti-NLRP3 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 3.104 primary fibroblasts were seeded on the outer face of polycarbonate membrane of cell culture inserts (Millipore, Sigma-Aldrich, Saint-Louis, MO, USA) and cultured for two days ...
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bioRxiv - Cell Biology 2021Quote: ... 3.104 primary fibroblasts were seeded on the outer face of polycarbonate membrane of cell culture inserts (Millipore, Sigma-Aldrich, Saint-Louis, MO, USA) and cultured for two days ...
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bioRxiv - Cancer Biology 2020Quote: ... 2uL of lysate (approximately 2ug) was spotted directly onto 0.2 um nitrocellulose membranes and blotted with primary antibodies against puromycin (Sigma MABE343 1:25,000 dilution) and vinculin (Abcam ab18058 ...
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bioRxiv - Neuroscience 2020Quote: ... Slots were washed with one volume of DPBS before washing the whole membrane with TBS and blocking with 5% skimmed milk powder (Sigma-Aldrich, Munich, Germany) in TBS for 1 hour ...
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bioRxiv - Systems Biology 2020Quote: ... 15 μg of proteins were taken from all samples and transferred to centrifugal filter units (Microcon-30kDa Centrifugal Filter Unit with Ultracel-30 membrane, Millipore Sigma, Billerica, MA). The samples were then processed following the filter aided sample preparation method [109] ...
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bioRxiv - Microbiology 2020Quote: ... were pooled and filtered through a vacuum system under sterile conditions using a membrane filter (pore size 0.22 μm; Millipore, GV CAT No GVWP04700). To prevent rupture ...
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bioRxiv - Plant Biology 2021Quote: ... Aliquots were run on a 4–12% SDS-PAGE gradient gel and electrophoresed proteins were transferred to a PDVF membrane (Immobilon-P, Millipore, Burlington, MA, USA). To facilitate detection ...
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bioRxiv - Microbiology 2020Quote: Czapek minimal media was prepared and sterilized and the solution of ellagitannin was sterilized separately by passing through a membrane filter (pore size 0.22 μm, Millipore Corporation, Bedford, MA, USA) and was added to Czapek Dox’s minimal medium to a final concentration of 1% ...
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bioRxiv - Microbiology 2021Quote: ... in water for injection containing 3% sterile-filtered sucrose using a 0.22-μm Millipak®-200 polyvinylidene fluoride membrane (PVDF; Millipore, Billerica, MA, USA) [11].