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Citations for Millipore Sigma :
8301 - 8350 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Four-day old adults were randomly collected during paralysis assays and stained with 1 mM X-34 (Sigma, Cat # SML1954) 10 mM Tris-HCl pH 8.0 for 2 hours as previously described (Link et al. ...
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bioRxiv - Microbiology 2024Quote: ... the virus titre was determined by plaque assay on MDCK cells as described previously using a final concentration of 1 µg/ml of TPCK trypsin (SIGMA)[22].
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bioRxiv - Plant Biology 2024Quote: ... water was removed and replaced with ROS assay solution (100 μM Luminol [Millipore-Sigma], 20 μg/ml horseradish peroxidase [Millipore-Sigma]), with or without immune elicitors ...
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bioRxiv - Pathology 2024Quote: ... the AI algorithm was validated against a standard low-resolution image-based caspase-3/7 assay in a proof-of-concept toxicity study where BBB organoids were treated with staurosporine (S5921, Sigma) to induce apoptosis27 ...
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bioRxiv - Neuroscience 2024Quote: ... or on coverslips (for immunocytochemistry assay) previously coated with poly-D-lysine (0.1 mg/mL) with neuronal plating medium (MEM supplemented (Sigma-Aldrich), 0.6% glucose and 1 mM pyruvic acid ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cytokine and chemokine levels of cell supernatants were measured using a MCYTOMAG-70K Mouse Cytokine Magnetic Multiplex Assay (Millipore Sigma) using a Luminex 200 analyzer instrument (Luminex Corp ...
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bioRxiv - Biochemistry 2024Quote: ... a reaction mixture consisting of 12nM FatA in assay buffer (50mM Tris HCl pH 8.0, 150mM NaCl, 12nM beta-casein (Sigma C6905)) was combined with test compounds in black 384-well microtitre plates (Greiner ...
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bioRxiv - Biophysics 2024Quote: To perform the osmotic-force-extension assay consecutive osmotic shocks were performed using commercial microfluidic plates (BA04, CellASIC, Millipore-Sigma) controlled by the ONIX microfluidic platform ...
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bioRxiv - Biophysics 2024Quote: To perform the osmotic-force-extension assay consecutive osmotic shocks were performed using commercial microfluidic plates (BA04, CellASIC, Millipore-Sigma) controlled by the ONIX microfluidic platform ...
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bioRxiv - Developmental Biology 2024Quote: ... the cartridge was removed from the incubator and each port was loaded as follows: For the mitochondrial stress test assay – 1st injection port – 20mM Oligomycin A (75351, Sigma); 2nd injection – 20uM FCCP (C2920 ...
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bioRxiv - Plant Biology 2020Quote: ... Thirty µg of total protein of each sample were separated on 15% SDS–polyacrylamide gels and transferred to ImmobilonTM PDVF membranes (Millipore Co., Bedford, MA, USA). The membrane was blocked for 1 h in PBS-T with 5% non-fat dried milk at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate [EMD Millipore AP160P], Rabbit IgG-HRP linked [EMD Millipore 12-348]) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... antibody was used as a pan-neuronal marker and membranes were reprobed with mouse monoclonal anti-β-actin antibody (1:10,000; Sigma, Saint-Quentin Fallavier, France) to confirm equal protein loading ...
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bioRxiv - Biochemistry 2021Quote: ... according to the Laemmli method [Laemmli U.K., 1970] and visualized with CBB (Coomassie Brilliant Blue R-250, Roth, Karlsruhe, Germany) or transferred to nitrocellulose membrane (Sigma-Aldrich, St. Louis, MO). The PageRuler Prestained Protein Ladder (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... protein samples were separated on a sodium dodecyl sulfate– polyacrylamide gel electrophoresis and transferred onto Immobilon-P PVDF membrane (EMD Millipore, Billerica, MA, USA). The membrane was incubated for 1 h in blocking buffer (Tris-buffered saline ...
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bioRxiv - Cell Biology 2021Quote: ... transferred to nitrocellulose membranes, and immunoblotted with α-Psd1 (1:1000, antibody kind gift from S. Claypool) or α-G6PDH (1:2000, Sigma-Aldrich A9521). Anti-rabbit antibody conjugated to DyLight 800 (1:10000 ...
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bioRxiv - Plant Biology 2021Quote: ... CPDs were subsequently labelled on the membrane by using a primary antibody against CPDs produced in mouse (H3 clone 4F6, Sigma Aldrich, St. Louis, USA). Detection was conducted by peroxidase coupled to the secondary antibody (Anti-Mouse IgG (whole molecule)-Peroxidase ...
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bioRxiv - Cell Biology 2022Quote: ... transferred to a nitrocellulose membrane, and probed with the following antibodies: anti-FLAG M2-HRP (HRP, horseradish peroxidase) conjugate (Sigma, A8592; dilution 1:10,000), rabbit anti-dynein heavy chain DYNC1H1 (Bethyl Laboratories ...
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bioRxiv - Biochemistry 2022Quote: The proteins were visualized by incubating membranes for 30 minutes in BCIP®/NBT Alkaline Phosphatase Substrate (B5655, SIGMAFAST™ BCIP®/NBT, Sigma-Aldrich). The intensities of the detected bands were analyzed with a FluorChem 8800 Imager (Alpha Innotech ...
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bioRxiv - Cancer Biology 2022Quote: Western blot analysis was done on a 4-12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore-Sigma, Immobiolon-P, Cat # IPVH20200) as previously described [1] ...
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bioRxiv - Microbiology 2020Quote: ... were boiled in SDS-PAGE loading buffer and subjected to SDS-PAGE and subsequently transferred to a polyvinylidene fluoride membrane (Millipore-Sigma, St. Louis, MO, USA) using a semidry transfer apparatus (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated by size using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a methanol-activated 0.2 μm PVDF membrane (Millipore Sigma, Burlington, MA; Cat #IPVH08100) using the Trans-Blot® Turbo Transfer System™ (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed 3 times with PBST and the protein bands were visualized by enhancing TMB substrate solution (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Immunology 2020Quote: ... In short, CEM-NKr cells (NIH AIDS Reagent Program, catalog #458) were double-labeled with PKH-26-cell membrane dye (Sigma-Aldrich, St. Louis, MO) and a cytoplasmic-staining dye (Vybrant CFDA SE Cell Tracer Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore; Thermo Fisher Scientific). PVDF membrane was treated with primary and secondary antibodies ...
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bioRxiv - Physiology 2022Quote: ... The supernatants were filtered through a 0.22 μm Cellulose Acetate filter tube (Corning® Costar® Spin-X® centrifuge tube filters cellulose acetate membrane; SIGMA-ALDRICH CLS8160) at 16000 g for 5 min and the filtrates were directly used for HPLC ...
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bioRxiv - Developmental Biology 2023Quote: ... The signal was compared to antibody stainings of loading controls (H3 and β-Catenin) and the membranes were quality controlled by Ponceau (Sigma Aldrich, #P7170-1L) staining ...
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bioRxiv - Plant Biology 2024Quote: ... The generated suspension was filtered through a nylon membrane (50 µm mesh) and 25 µL of propidium iodide solution (1 mg mL-1; Sigma-Aldrich, St. Louis, MO) and 5 mL RNAse (Amresco ...
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bioRxiv - Molecular Biology 2023Quote: ... One membrane was used to probe for HURP (HURP antibody described in this paper, 4 μg/mL) and γ-tubulin (Sigma: T6557, 1:1000). The second membrane was used to probe for XMAP215 (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were transferred to polyvinylidenedifluoride membranes that were blocked in 5% non-fat milk or 5% BSA (Sigma-Aldrich, St. Louis, MO, USA) in Tris-buffered saline with 0.1% Tween 20 (TBS-T) ...
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bioRxiv - Biochemistry 2022Quote: ... Actin retained on the resin was eluted with 12 mL G-buffer without actin and all elution fractions were combined and concentrated with a 30 kDa cut-off membrane (Millipore Sigma, St. Louis, MO) to 0.9 ml ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of the membrane-proximal PLIN3 constructs was repressed by cultivating cells in the presence of doxycycline (1 μg/ml; Sigma-Aldrich, St Louis, MO) and expression was induced by shifting cells to media lacking doxycycline ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with TBST and incubated with blocking buffer containing Rabbit IgG HRP Linked Whole Ab (Sigma cat no. GENA934-1ML) at 1:5000 for two hours with gentle mixing ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were resolved on sodium dodecyl sulfate polyacrylamide gels under denaturing conditions and blotted onto PVDF membranes (Immobilon-P; Millipore, Burlington, MA, United States) by wet transfer for 1–2 h at 90 V ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were treated with 0.2 M NaOH for 30 minutes at 30°C before probing for PP2AC subunit (Millipore Sigma 05-421, clone 1D6).
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bioRxiv - Cancer Biology 2024Quote: ... Crystal violet was then eluted from the membranes by adding 500 µl of 33% v/v acetic acid (Millipore Sigma, Cat. No. AX0073-75) solution and shaking for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The gel was transferred to a positively charged Amersham Hybond N+ membrane (Cytiva, Cat. No. RPN303B) overnight using capillary action with 20x SSC buffer (Sigma-Aldrich, Cat. No. 33220). Following transfer ...
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bioRxiv - Cell Biology 2024Quote: ... Each lysate containing the same amount of proteins was separated on 8–12% SDS-polyacrylamide gels (PAGE) and electro-transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA, #IPVH00010). The membrane was incubated at 4°C overnight with the following primary antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was blocked in 5% non-fat milk in 1X TBST and detected with mouse monoclonal anti-HA (H9658, Sigma-Aldrich, St. Louis, MO) and anti-actin (A0480 ...
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bioRxiv - Bioengineering 2024Quote: ... and transfected 24 h with EGFP-fused mouse brain membrane protein IgSF8 (Uniprot G3UYZ1) using X-treme GENE 9 DNA Transfection Reagent (Sigma Aldrich, cat. no. 6365787001). Cells were lysed for 2 h at 4 °C on a rocking platform with 0.5 mL per well of 20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... One gel was stained with coomassie brilliant blue to reveal MBP and MBP-BIM1 and the other was transferred to a PVDF membrane perform western blot analysis using primary antibody anti-HIS made in mouse (SIGMA, H1029 dilution 1:3000) and the secondary antibody anti mouse IgG used for the CoIPs.
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated overnight at 4 °C on a rocker with the following antibodies in Blocking buffer: β-actin (Sigma-Aldrich, Gillingham, UK A5441), LC3B (Cell Signaling Technology ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich, MA, USA), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Molecular Biology 2023Quote: ... TUNEL staining was performed using a commercial kit (In Situ Cell Death Detection Kit, TMR (Sigma, 12156792910)) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... osteoclasts were evaluated by TRAP staining using a commercially available kit (Acid Phosphatase TRAP kit, Sigma–Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... primary antibodies were removed with PBS and cells were subjected to the Duolink™ Proximity Ligation Assay protocol according to manufacturer instructions (Sigma: DUO92008 ...
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bioRxiv - Cancer Biology 2021Quote: Cell viability was determined using a commercial 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) viability assay (Sigma Aldrich, Rehovot, Israel). That assay was used to determine how highly 4T1 proliferation was stimulated by Norepinephrine (NE ...
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bioRxiv - Genetics 2021Quote: ... Telomere length was measured by two orthogonal methods: 1) qPCR8 and 2) telomere restriction fragment (TRF) analysis using TeloTAGGG Telomere Length Assay (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2022Quote: All enzyme activity were determined by measuring the release of phosphate and formation of a green complex formed between malachite green oxalate with phosphomolybdate and using the malachite green assay as previously described (55) with slight modifications and manufacturer specifications (Sigma-Aldrich). 40 μL of M87SpastinA2C and VPS4BS2C in ATPase buffer (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... ATP-hydrolysis rates were measured over the first ~2 min by monitoring the loss of absorbance at 340 nm using a coupled NADH–ATP assay (Burton et al., 2001) with 5 mM ATP (Sigma-Aldrich), pyruvate kinase (Sigma-Aldrich ...