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Citations for Millipore Sigma :
7951 - 8000 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cholesterol solubilization assays were performed by supplementing NGM containing 5 μg/mL cholesterol with either 0.1% Tergitol (Sigma-Aldrich) or 0.1% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... The mitochondrial import assay results were analysed by visualizing the proteins with the Coomassie stain (PhastGel Blue R, Sigma). The proteins were then fixed (20% ethanol and 10% acetic acid) ...
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bioRxiv - Evolutionary Biology 2020Quote: Extracellular protease production of clones from populations at generations 870 was determined by FITC-caseinase assays (Sigma-Aldrich PF0100) as previously described [21] ...
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bioRxiv - Neuroscience 2022Quote: ... AP-Sema3A concentration was determined by AP activity assay (using AP substrate buffer para-nitro-phenyl phosphate, Sigma Millipore) (Mlechkovich et al. ...
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bioRxiv - Neuroscience 2022Quote: ... AP-Sema3A concentration was determined by AP activity assay (using AP substrate buffer para-nitro-phenyl phosphate, Sigma Millipore) (Mlechkovich et al. ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Cells were cultured for 24 h and intracellular RT activity was determined with colorimetric reverse transcriptase assay (Sigma Aldrich) according to manufacturer’s instruction.
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bioRxiv - Genomics 2020Quote: The isolate was tested for presence of a cytochrome oxidase using the 70439 Oxidase assay (Sigma-Aldrich, MO, USA) according to instruction provided by the manufacturer ...
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bioRxiv - Bioengineering 2020Quote: ... Digestate was then assayed for sulfated-GAG content using 1,9-Dimethyl-Methylene Blue assay with a chondroitin sulfate standard (2.5-25 µg/mL; Sigma-Aldrich).(34 ...
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bioRxiv - Biochemistry 2020Quote: ... Cell viability was determined by MTT assay and measured at 570 nm according to the manufacturer’s instructions (EMD Millipore). All experiments were performed in triplicate ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was assessed 84h after the treatment start with Resazurin cell viability assays (16 µg/ml, Sigma-Aldrich). Dose response curves were simulated by nonlinear regression models and IC50 values were calculated using PRISM 9 (GraphPad Software Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was assessed 84h after the treatment start with Resazurin cell viability assays (16 µg/ml, Sigma-Aldrich) normalized to the respective controls (vehicle alone) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates containing FLAG-tagged motors were diluted in assay buffer [AB: P12 buffer (12 mM PIPES (Sigma P-1851), 1 mM EGTA (Sigma E-4378) ...
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bioRxiv - Cancer Biology 2020Quote: ... Viability was evaluated with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) colorimetric assay (Sigma, #M5655).
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bioRxiv - Biochemistry 2021Quote: ... at 25 °C with a volume of 85 μl per well in BLI buffer (assay buffer containing 0.2% (w/v) BSA (Sigma)) ...
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bioRxiv - Plant Biology 2020Quote: ChIP-seq assays were performed on 14 days old in vitro shoot seedlings using anti-H3K27me3 (Millipore 07-449) or anti-H3K27me1 (Millipore 07-448) ...
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bioRxiv - Microbiology 2020Quote: ... Concentration of stock was determined by plaque assay (described below) on MA-104 cells overlaid with M199 (Sigma-Aldrich) + 0.5% agarose and evaluated 5 days later using neutral red ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: We measured the glucose concentrations in cell culture supernatant by a glucose uptake assay test according to the manufacturer’s protocol and previous researches manufacturer’s (GAHK20, Sigma, USA). Results normalization was carried out according to the number of cells.
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bioRxiv - Microbiology 2020Quote: ... while for the plaque assay the infected cell monolayer was overlaid with Leibovitz-15 (L15) media supplemented with 0.9% agarose (DNA grade, Sigma), antibiotics and 2 μg mL−1 TPCK Trypsin ...
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bioRxiv - Biochemistry 2022Quote: ... or BT474 cells were scraped on ice in 200 µL ice-cold IDH Assay Buffer (Sigma, Catalog Number MAK062A), centrifuged at 13,000 x g for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.001% SDS to the volume of 90 µl per measurement and supplemented with 10 µl of 0.5mM proteasome substrates in the assay buffer: Substrate III (Suc-LLVY-AMC, chymotrypsin-like activity, Millipore), Substrate IV (Z-ARR-AMC ...
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bioRxiv - Physiology 2022Quote: Cell viability was measured by the colorimetric assay showing reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) as previously described (Denizot and Lang ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich) as described [29].
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bioRxiv - Microbiology 2021Quote: The following small molecule inhibitors and antibodies were used for TEER and EGL inhibition assays: ATN-161 (Sigma, SML2079), SB 431542 hydrate (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... and trypsin concentration was quantified using an enzymatic assay with Nα-Benzoyl-L-arginine ethyl ester (BAEE, Sigma-Aldrich) exactly according to Rocklin et al (Rocklin et al ...
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bioRxiv - Microbiology 2021Quote: ... The cell-free supernatants were stored at –80°C for total protein quantification using the BCA protein assay (Sigma).
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bioRxiv - Microbiology 2020Quote: ... Cell viability was determined by MTT assay and measured at 570 nm according to the manufacturer’s instructions (EMD Millipore). All experiments were performed in triplicate ...
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bioRxiv - Neuroscience 2021Quote: Proximity ligation assay (PLA) was performed in primary hippocampal neurons and N2a cells according to the manufacturer’s instructions (Duolink, Sigma). Until the PLA probe incubation step ...
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bioRxiv - Neuroscience 2022Quote: One cerebral hemisphere (without the cerebellum or olfactory bulb) was homogenized in radioimmunoprecipitation (RIPA) assay buffer (Sigma-Aldrich; R0278) containing Pierce protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... PLA assay was performed using custom labelled antibodies and the Duolink In Situ Detection Reagents Red (Sigma-Aldrich, DUO92008) as described by the manufacturer but with slight modifications ...
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bioRxiv - Systems Biology 2020Quote: ... Metabolic hormones were measured using MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay (HMHEMAG-34K, Millipore). Cardiovascular risk markers in plasma were measured using MILLIPLEX MAP Human Cardiovascular Disease (CVD ...
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bioRxiv - Pathology 2021Quote: Proximity ligation assay (PLA) was performed according to the manufacturer’s instructions (Duolink In Situ detection reagent-Red, Sigma Aldrich). Briefly ...
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bioRxiv - Physiology 2020Quote: ... the protein content of each sample was quantified using the Bradford assay (B6916, Sigma Aldrich, Saint Louis, MI, USA) with protein standards made from bovine serum albumin (ALB001.25 ...
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bioRxiv - Physiology 2020Quote: ... and 120-minutes thereafter using a colorimetric assay (PGO enzyme preparation and dianisidine dihydrochloride, Sigma-Aldrich, St. Louis, MO). Area under the curve measurements were calculated using the trapezoidal method [32].
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bioRxiv - Physiology 2021Quote: ... complete culture medium was exchanged for pre-warmed Seahorse Assay medium supplemented with 10mM glucose (Sigma-Aldrich, Poole, UK), 1mM sodium pyruvate (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Total protein concentration was determined using the Bradford assay with bicinchoninic acid (BCA) reagent (Millipore Sigma, St. Louis, MO). Total protein (40 μg ...
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bioRxiv - Genomics 2021Quote: ... and solubilized in IB buffer MB and HS fractions were estimated by Bradford assay or Direct Detect spectrophotometer (Millipore). Protein samples were solubilized in Laemmli buffer with 50 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... was prepared in USP30 activity assay buffer (50 mM Tris base, pH 7.5, 100 mM NaCl, 0.1 mg/ml BSA (Sigma, A7030), 0.05% Tween 20 ...
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bioRxiv - Pathology 2021Quote: ... of fresh frozen villous placental tissue samples were homogenized into the assay lysis buffer containing 0.1% Triton-X 100 (Sigma) and further processed per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were cultured for 72 h and then cell death was measured using the tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-terazolium-5-carboxanilide (XTT) assay (Sigma) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... Proliferation at corresponding time points was assessed by MTT (Methylthiazolyldiphenyl-tetrazolium bromide) assay according to the manufacturer’s recommendations (Sigma). For each time point ...
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bioRxiv - Cancer Biology 2022Quote: The PDX tissues were lysed in radioimmunoprecipitation assay (RIPA) buffer with protease and phosphatase inhibitor cocktail (Sigma-Aldrich: #P8340). The protein concentrations were calculated by BCA assay ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... as for the “Group two-choice assay” except using a single 3 cm diameter Petri dish containing 0.67% agarose and 150 mM sucrose (Sigma) onto which a non-accessible container covered with a fabric gauze and a perforated cap of a 15 ml Falcon tube (diameter 1 cm ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were assayed for secreted chemokines and cytokines using the Milliplex 38-plex Human Cytokine/Chemokine Assay (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The effect of CAPG depletion on viability of KO and siRNA-treated cells was assessed at several timepoints using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (11465007001; Sigma-Aldrich) per manufacturer directions ...
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bioRxiv - Microbiology 2022Quote: ... Cells from these cultures were then used for batch culture growth kinetic assays in Tibbles–Rawling Minimal Medium13,30 (TR medium) containing 0.1% (weight/volume) alginate (Sigma Aldrich). Sterile alginate solution was prepared using nanopure water and filter sterilized using 0.40 μm surfactant-free cellulose acetate filters (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... was propagated in Vero cells and titers were determined by plaque assay on Vero cells using 1% methylcellulose (Sigma)(Stojdl et al. ...
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bioRxiv - Immunology 2022Quote: Cytokines were measured in duplicate plasma samples using an HCYTMAG-60K-PX41 Premixed 41 Plex - Immunology Multiplex Assay (Millipore). The results were subsequently analyzed using GraphPad Prism Version 9.2.0 software (San Diego ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 cells (for proliferation assay) or 1000 cells (for 3D uptake) per 20μl drop containing 4.8mg/ml methylcellulose (Sigma-Aldrich) and 20μg/ml soluble collagen I (BioEngineering ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were washed twice with 100μL/well HBSS prior to addition of 80μL/well assay buffer (HBSS (Sigma H8264) + 0.1% BSA) ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were blocked for 1 h in AD before addition of assay samples for 1 h followed by incubation with 1/20,000 dilution Goat anti-human Fc-HRP (Sigma). All steps were conducted at 37°C and separated by three washes with PBS-T (0.05% Tween-20) ...