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Citations for Millipore Sigma :
8351 - 8400 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: The sections from two biological replicate experiments were stained using a Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay (Millipore S7165), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Guanosine nucleotide hydrolysis was assessed through synthetase assays performed as above using guanosine 5’-β-thio-diphosphate trilithuim salt (GDPβS) as the only nucleotide phosphoacceptor (Millipore Sigma). The reactions were caried out for 60 mins at 37°C and the spots were autoradiographed as reported above.
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bioRxiv - Microbiology 2021Quote: ... moscoviensis was assessed by measuring total cell protein with the enhanced test tube protocol of the BCA protein assay (Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was assayed for glucose concentration using glucose oxidase/peroxidase colorimetric glucose assay with 45 μg/ml o-dianisdine dihydrochloride (Sigma-Aldrich), and absorbance measured at 450 nm ...
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bioRxiv - Biochemistry 2021Quote: Growth for the heme trafficking dynamics assay was accomplished by culturing HS1-expressing cells with or without 500 μM SA (Sigma-Aldrich) in SC media lacking leucine ...
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bioRxiv - Immunology 2021Quote: ... the cells were lysed for 30 minutes on ice in Radioimmunoprecipitation assay (RIPA) buffer (Sigma Aldrich, St. Louis, MO, USA, #R0278) supplemented with cOm-plete™ EDTA-free Protease Inhibitor Cocktail (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... The concentration of inorganic phosphate released by hydrolysis of ATP was measured by tracking absorbance at 620 nm of malachite green binding to phosphate ions using the ATPase/GTPase activity colorimetric assay (Sigma-Aldrich) as described (14).
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bioRxiv - Cell Biology 2021Quote: Transferrin uptake assays were performed by serum-starving cells in DMEM/f12 with 0.5% (w/v) BSA (Sigma Life Sciences A9647) for 30 minutes followed by adding fresh DMEM/f12/ BSA with alexa-fluor 647-labeled transferrin at a concentration of 10 micrograms/milliliter (Jackson Immunoresesarch 009-600-050) ...
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bioRxiv - Cell Biology 2021Quote: ... were equilibrated in 500 μl of assay buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Microbiology 2020Quote: ... J774A.1 cells were maintained in DMEM supplemented with heat inactivated serum and the assay was complemented with 1 μg/mL C3 (Sigma, C2910) or C3b (Sigma ...
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bioRxiv - Microbiology 2020Quote: Cell lysates were prepared using Radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with protease inhibitors (cOmplete Protease inhibitor cocktail, Sigma-Aldrich, 11697498001) and 50mM 1,4-Dithiothreitol (DTT) ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were homogenized in 10 volumes of ice-cold radioimmunoprecipitation assay buffer containing protease inhibitor cocktail (Sigma-Aldrich, St. Louis, Missouri), phosphatase inhibitor cocktail (PhosStop ...
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bioRxiv - Physiology 2020Quote: ... Samples were then incubated for 10 min at room temperature with assay buffer including glucose oxidase (0.25 U/mL, Sigma-Aldrich, G7141) and horseradish peroxidase (0.17 U/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... The effect of the anti-angiogenic compounds were measured using the 3-[4,5-dimethylthiazol- 2yl]-diphenyltetrazolium bromide (MTT) colorimetric assay (Sigma, Lyon, France) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell lysates of cultured cells or MDS BM MNCs were prepared with standard radioimmunoprecipitation assay buffer in the presence of protease (Sigma, cOmplete) and phosphatase (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... ATP to be used for ATPase activity assays was purified after diluting 20 μCi ATP γ-P32 (Perkin-Elmer) in 100 µl of 100 mM unlabeled ATP (Sigma) by passing through a 3 ml P2 resin size-exclusion column equilibrated with a buffer containing 50 mM HEPES·KOH pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: Two immortalized cell lines were used to validate the ACE2 receptor, TMPRSS2 and NRP1 assays: Human oligodendroglioma cells (HOG, Cat# SCC163) were obtained from Millipore Sigma-Aldrich (Billerica ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells (4×104) were washed twice with PBS and re-suspended with 175 μL of assay medium containing bicarbonate-free RPMI1640 (Sigma-Aldrich) supplemented with 25 mM glucose ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant human IL6 protein had been diluted in assay diluent (PBS with 5 mM EDTA, 100 μg/ml single-stranded salmon sperm DNA (Sigma Aldrich), 0.1% BSA ...
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bioRxiv - Physiology 2022Quote: PKA activity assay was performed using whole cell lysate from iPSC-CMs treated with either PBS or 10μM forskolin (Sigma-Aldrich, F6886) for 30min at 37°C prior to assay ...
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bioRxiv - Cell Biology 2022Quote: ... of SPTAN1-kRas and SPTAN1-Raf1 were identified using the Duolink® proximity ligation assay (PLA) in situ red for mouse/rabbit antibodies (DUO92101, Sigma). THP-1 cells were fixed onto microscope slides and permeabilized as outlined in the ICC/IF section ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transferred to black-walled 96-well plates (Falcon) 4 h before the assay and incubated with EBM or 200nM Staurosporine (Sigma Aldrich) for 4 h ...
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bioRxiv - Bioengineering 2022Quote: ... GAG content in co-cultured constructs was measured using a dimethyl methylene blue (DMMB) assay [79] with shark cartilage chondroitin sulfate (C4284, Sigma-Aldrich) as a reference ...
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bioRxiv - Neuroscience 2022Quote: ... United States) according to the manufacturer’s instructions.\Individual assays were performed for total tau (Tau-5, # 577801, Millipore, Australia, 1:50), h-tau (p-Ser202/Thr205-tau (AT8) ...
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bioRxiv - Neuroscience 2022Quote: ... A portion of the frozen somatosensory cortex\tissue was homogenized in radioimmunoprecipitation assay (RIPA) buffer with protease and phosphatase inhibitors (SigmaFast, S8820, Sigma-Aldrich, USA and PhosStop ...
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bioRxiv - Bioengineering 2022Quote: ... Protein content was measured by the BSA (cells) or microBSA (EVs) protein assay according to the manufacturer’s instructions (Sigma Aldrich, USA).
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bioRxiv - Cancer Biology 2022Quote: ... with 1X protease and phosphatase inhibitor cocktail (Fisher, USA), and 1X DTT (Fisher, USA) and was quantified via a bicinchoninic acid assay (Sigma, USA). The lysates were then separated using SDS–PAGE and electroblotted onto polyvinylidene difluoride membranes ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed twice in cold sterile phosphate-buffered saline (PBS) and lysated using ice-cold radio-immunoprecipitation assay (RIPA) buffer supplemented with 1% protease inhibitor cocktail (Sigma-Aldrich) and 1% phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... this TREx/ItypOrco-expressing cell line was tested in a fluorescent calcium assay (described below) against the Orco agonist VUAA1 (>98 % purity, Sigma-Aldrich), which directly activates the Orco protein in nearly all insect species tested to date43,48,65 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... retina and choroid tissues dissected out from the L-CNV and untouched control mice (four eyes) were lysed for 20 min on ice in radioimmunoprecipitation assay (RIPA) buffer (Sigma #R0278) with protease inhibitors (cOmplete mini ...
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bioRxiv - Molecular Biology 2022Quote: Cell proliferation assays were performed with insect cell lines (Aag2 [A. aegypti], AsE01 [A. suspensa], and S2 [D. melanogaster]) using the WST-1 assay (Sigma-Aldrich). Cells (Aag2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The expression level of each enzyme was quantified using radioactive leucine incorporation assays as previously described.(Jewett et al., 2008) All reagents and chemicals were purchased from Sigma-Aldrich unless otherwise specified.
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bioRxiv - Cancer Biology 2020Quote: ... Luciferase and Renilla activity were determined in a Tecan plate luminometer with freshly reconstituted assay reagents (0.5 mM D-Luciferin (Sigma-Aldrich, L9504), 20 mM tricine ...
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bioRxiv - Pathology 2020Quote: ... The amount of attached cells (from the washed plate) and the total cells (from the unwashed plate) was determined using XTT assay (Sigma, # 11465015001) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... and MILLIPLEX MAP Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 1-Cardiovascular Disease Multiplex Assay (#MCVD1MAG-77K-02, all from Millipore Sigma) were used to analyze cytokines and chemokines from mouse plasma ...
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bioRxiv - Cell Biology 2020Quote: ... Cell counts and viability were checked at the time of EV harvest using the trypan blue exclusion assay (0.4% Trypan blue solution; SIGMA; cat T8154).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Larvae were collected and prepared for the assay as detailed above except that they were kept in food with 40 μM all trans-retinal (ATR, Sigma-Aldrich) and in dark for 72 hours ...
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bioRxiv - Neuroscience 2021Quote: ... The biochemical assay of GCase activity was carried out in a buffer containing 4-methylumbelliferyl beta-d-glucopyranoside substrate (Cat. M3633, Sigma Aldrich); after 1 hour at 37°C the reaction was stopped with 0.25 M glycine buffer pH 10.4 and the fluorescence emission of the 4-methylumbelliferyl generated (4-MU ...
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bioRxiv - Plant Biology 2020Quote: Spin trapping assays with the spin probe 4-pyridyl-1-oxide-N-tert-butylnitrone (4-POBN; Sigma-Aldrich, St. Louis, USA) to detect the formation of hydroxyl radicals24 were carried out using cyanobacterial cells (OD730 = 0.65 ...
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bioRxiv - Microbiology 2021Quote: ... permeabilized with 0.2% Triton X-100 (in Phosphate Buffered Saline [PBS]) and subjected to an immunofluorescence assay (IFA) using a monoclonal antibody (MAb) anti-ACE2 (Sigma-Aldrich), and then incubated with a goat anti-rabbit IgG (goat anti-rabbit IgG ...
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bioRxiv - Microbiology 2020Quote: Alkaline phosphatase activity (APA) was evaluated through fluorometric assay following the hydrolysis of the substrate (S) 4-methylumbelliferyl phosphate (MUF-P, Sigma-Aldrich) to 4-methylumbelliferyl (MUF) ...
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bioRxiv - Microbiology 2020Quote: ... For the phenotypic and proteomic assays after treatment with hydrogen peroxide a final concentration of 5 mM and 10 mM were selected (Sigma-Aldrich). For susceptibility assays C ...
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bioRxiv - Immunology 2021Quote: ... obtained from the NA activity assay of the PV to a standard curve of 4-Methylumbelliferone sodium salt 4-MU(SS) (Sigma M1508). NA activity is based on the production of 4-MU over time (60 minutes at 37 °C for the standard assay) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged and protein concentration measured using a bovine serum albumin standard curve and a bicinchoninic acid (BCA) assay (Sigma-Aldrich). The WES capillary electrophoresis system (ProteinSimple ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells grown in hypoxic chambers were frozen at the time points mentioned in the results section and harvested all together by lysing in Radioimmunoprecipitation assay buffer (RIPA buffer) containing 1× protease inhibitor cocktail (Sigma-Aldrich). For each sample ...
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bioRxiv - Immunology 2022Quote: ... Cell number and viability were examined each day in triplicate wells using trypan blue exclusion and cell proliferative assay (# Cat. no. T8154, Sigma-Aldrich). The assay was performed for 10 d post-seeding i.e. ...
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bioRxiv - Cell Biology 2022Quote: ... pulverized in liquid nitrogen and protein was extracted using a 1x radioimmunoprecipitation assay buffer (50mM Tris-HCl pH 8.0, 150 mM NaCl, 1% Nonidet®40 Substitute (74385; Sigma-Aldrich), 0.5% sodium deoxycholate ...
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bioRxiv - Bioengineering 2022Quote: ... Hydroxyproline content as a measure for collagen was quantified using a chloramine-T assay (Huszar et al., 1980) with trans-4-hydroxyproline (H54409, Sigma-Aldrich) as reference ...
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bioRxiv - Cell Biology 2022Quote: ... The following small molecules required for the MTS assay were ordered individually and 10 mM stocks prepared and stored at −20°C: (CCCP, Sigma, C2920), (Oligomycin ...