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Citations for Millipore Sigma :
8251 - 8300 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and in vitro assays on SK-MEL-28 cells were as follows: 4-Hydroxyanisole (4-HA, Sigma-Aldrich, MO, USA), the canonical Wnt pathway antagonist IWR-1 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was used for FLAG purification in pull-down assays with Anti-Flag® M2 Magnetic Beads (Sigma-Aldrich), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: The cellular viability of the brain-like tissues was tested using a lactate dehydrogenase assay (LDH) (Sigma, cat.no. MAK066-1KT) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: The etiolated seeding triple response assay was performed with sterilized seeds on various concentrations of 1-aminocyclopropane-1-carboxylic acid (ACC, Sigma) plates (0 ...
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bioRxiv - Microbiology 2024Quote: ... Cytokines secreted by primary CF-HBECs were analyzed by the MILLIPLEX MAP Human Cytokine/Chemokine 48-Plex cytokine assay (Millipore). Cytokines were measure in the basolateral solution only since CF-HBEC were grown in air-liquid interface culture and there was insufficient apical fluid to measure cytokines.
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bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... Sigma-Aldrich)-coated 6-well plates (200,000 cells/well) for western blot experiments and in 96-well plates (10,000 cells/well) for MTT assay (#M2003, Sigma-Aldrich).
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bioRxiv - Immunology 2024Quote: ... After 24hrs supernatant was collected and the cytokine expression was analyzed by luminex bead array assay (Millipore Sigma, Burlington, MA). In separate cultures ...
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bioRxiv - Immunology 2024Quote: ... Each 1 ml collected fraction was analyzed for protein concentration using a Nanodrop spectrophotometer (absorbance at 280 nm) and pooled three fractions were further analyzed for protein concentration using Bradford assay (Sigma) and used to measure biological activity to induce Arg1 expression in RAW264.7 macrophage cell line ...
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bioRxiv - Microbiology 2024Quote: gGT-activity was determined by a colorimetric assay based on the release of p-nitroaniline from the substrate gGpNA (Sigma). The method was adapted from Meister et al ...
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bioRxiv - Immunology 2024Quote: WB analysis of protein levels was performed on whole-cell lysates prepared by lysis in radioimmunoprecipitation assay buffer (Sigma-Aldrich) supplemented with Benzonase (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... The ASA content of tomato fruits was determined using a titration assay and standard curve with commercial ascorbic acid (S88476, Sigma) and 2,6-dichloroindophenol (119814 ...
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bioRxiv - Biochemistry 2023Quote: ... the substrate stocks were diluted with assay buffer (50 mM Tris-HCl, pH 8.0) additionally containing 1 mg ml-1 gum arabicum (Sigma Aldrich). All values were determined in quadruplicates and corrected for autohydrolysis using the experimentally determined pH-dependent extinction coefficient of p-nitrophenol in microtiter plates (ε = 9.3024 mL µmol-1) ...
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bioRxiv - Biochemistry 2023Quote: ... The final experimental mix was prepared on ice: assay buffer supplemented with oxygen scavengers (0.1 mg mL-1 catalase (Sigma-Aldrich), 1 mg mL-1 glucose oxidase (Serva)) ...
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bioRxiv - Physiology 2024Quote: ... Triglyceride concentrations were determined using a spectrophotometric assay with a 4:1 Free Glycerol Agent / Triglyceride Agent solution (Cat#: F6428 & T2449, Sigma, Free-Glycerol reagents and Triglyceride ...
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bioRxiv - Microbiology 2023Quote: ... and the decanted supernatant was quantified with a microplate Bradford assay per manufacturer’s instructions (Sigma-Aldrich, Sant Louis, MO, USA).
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bioRxiv - Bioengineering 2023Quote: The viability of the encapsulated islets was assessed after 24 days of incubation using two different methods: the resazurin assay for quantification (Sigma) and the Live/Dead assay for visualization (Life Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... 50 µM sphinganine (2.5 µL 500 µM sphinganine in Assay Buffer containing 10% ethanol) and 50 µM palmitoyl-CoA (Sigma-Aldrich) (2.5 µL 500 µM palmitoyl-CoA in Assay Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich), 0.2 mg/ml glucose oxidase (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability in 2D monolayer cultures ± treatment was quantified using an XTT assay according to the manufacturer’s protocol (Sigma-Aldrich). Briefly ...
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bioRxiv - Plant Biology 2024Quote: Ethylene triple response assay was performed with sterilized seeds on MS plates containing various concentrations of 1-aminocyclopropane-1-carboxylic acid (ACC, Sigma) (0 ...
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bioRxiv - Cell Biology 2022Quote: In vitro histone acetylation assays were performed on 25 μg of mitotic chromatin (isolated as described above and quantified using the bicinchoninic acid colorimetric assay system (Sigma)) in 50 mM Tris-HCL ...
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bioRxiv - Cell Biology 2022Quote: ... HAT activity was quantified by: i) a colorimetric assay quantifying the release of Co-A using DTNB (2-nitrobenzoic acid) (Sigma) by measuring the absorbance at 412nm ...
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bioRxiv - Bioengineering 2022Quote: MSC derived small EVs were characterized for protein concentration using the bicinchoninic acid (BCA) assay (Sigma Aldrich, Oakville, ON, Canada). Nanoparticle tracking analysis was performed using ZetaView (Particle Metrix ...
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bioRxiv - Microbiology 2022Quote: Total protein was extracted from LT toxin-treated (100ng/ml) human differentiated enteroid monolayers (235D line) and untreated control using radioimmunoprecipitation assay (RIPA) buffer (Sigma) containing protease inhibitor cocktail ...
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bioRxiv - Plant Biology 2022Quote: ... the water was removed and replaced with ROS assay solution (100 μM Luminol [Millipore-Sigma], 20 μg/mL horseradish peroxidase [Millipore-Sigma] with or without immune elicitors) ...
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Synthetic Biology 2023Quote: HEK293T-LP cells sourced from Matreyek et al38 were plated into a 48 well plate according to the plate reader assay and treated with 0 to 2 ng/μl dox (Sigma) to induce BFP expression ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: Enzymatic Assays from skeletal muscle tissues were done from muscle lysates prepared in the assay buffer as per the manufacturer’s protocol (Sigma-Aldrich). Serum calcium levels were measured using calcium estimation kit (Abcam #ab102505 ...
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bioRxiv - Cancer Biology 2022Quote: ... No HCYTOMAG-60K) were quantified using Milliplex MAP Luminex microbead assays according to the manufacturer’s instructions (Merck Millipore, Darmstadt, DE). Samples were analyzed without dilution in duplicate ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected after centrifuging at 4°C for 10 minutes and the protein amounts were determined by BCA assay (Sigma). The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then incubated on a shaker for 10 minutes in radio-immunoprecipitation assay (RIPA) buffer (#R0278, Sigma, UK) and 1% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... were diluted in pre-warmed XF assay media to achieve the following final concentrations in the cell culture well: 1.5 μM Oligomycin A (Sigma, 75351); 1 or 0.5 μM FCCP (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration in the remaining supernatant was assessed using a Bradford reagent assay according to manufacturer’s protocol (Sigma-Aldrich - B6916), and normalized on collected fecal weight and on animal weight.
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bioRxiv - Immunology 2024Quote: ... The migrated neutrophils in the apical compartment were lysed with 0.5% Triton X-100 and neutrophil MPO activity was quantified using a colorimetric assay using citrate buffered 2,2’-azino-bis(3-ethylbenxothiazoline-6-sulfonic acid) diammonium salt (ABTS) solution (Sigma-Aldrich). After 10 min of incubation ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002 ...
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bioRxiv - Cancer Biology 2024Quote: ... The tubes were centrifuged at 6,000 rpm and the supernatants were collected for cytokine analysis by bead-based multiplex assay (MILLIPLEX MAP Mouse High Sensitivity T Cell Panel, Millipore) following the manufacturer protocol27.
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bioRxiv - Cell Biology 2024Quote: ... Knockdown efficiency and p130Cas over-expression levels were confirmed by Western blotting of cell lysates in radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich] ...
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bioRxiv - Plant Biology 2024Quote: ... the amount of protein in the sample was quantified by BCA assay using the Bicinchoninic acid solution (Sigma-Aldrich B9643) and CuSO₄.5H₂O ...
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bioRxiv - Physiology 2024Quote: ... after which the reaction mixture was used to measure ATP levels via a luciferase/luciferin based assay system according to manufacturer’s protocol (FLAA - Sigma Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... Primers were designed to target Nr3c1 exon 17 using PyroMark Assay Design software and synthesised by Sigma Aldrich (HPLC purified). The reverse primer was 5’ end biotin labelled ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed with PBS-T and 25 μL of detection antibody diluted in assay buffer added to each well: goat anti-human IgG-AP (1:1,000) (Sigma Aldrich) or goat anti-human IgG3-AP (1:1,000 ...
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bioRxiv - Immunology 2024Quote: The purified neutrophils (Ly6+ cells) were lysed in radioimmunoprecipitation assay (RIPA) buffer containing a protease inhibitor cocktail (Sigma-Aldrich, MO) and denatured at 95°C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Plasma samples from in vivo studies were previously diluted 1:20 while sera from field studies were diluted 1:10 in assay buffer (PBS-1% BSA-0.05% Tween 20, (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2024Quote: ... MCP1 and TNFα protein concertation in WT and KO-Hmox1 mice blood serum was measured by using Luminex (MILLIPLEX MAP Mouse Cytokine/Chemokine Premixed 32 Plex, Mouse Cytokine/Chemokine Magnetic Bead Premixed 32 Plex and the custom assay panel, Millipore). Assays were performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Australia) and the viability was assessed by the resazurin-based Alamar Blue assay (#R7017, Sigma-Aldrich, St. Louis, MO, USA) and the florescence intensity in each well was measured after 90 mins using a bottom-reading florescent plate reader (CLARIOstar Plus ...
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bioRxiv - Immunology 2024Quote: ... and perfusate levels of 71 analytes using two separate panels as per the manufacturer’s instructions for use: (1) the Human Cytokine 48-Plex Discovery Assay® (HD48; Millipore MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel Immunology Multiplex Assay ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then diluted to 1 mg/ml and processed by Eve Technologies using the Mouse High Sensitive 18-Plex Discovery Assay according to the manufacturer’s protocol (Millipore Sigma).
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bioRxiv - Bioengineering 2024Quote: ... and polyIC incubation was evaluated using the MTT (3-(4, 5dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). The cells were seeded into 96 well-format cell culture plates at a seeding density of 8ξ103 cells per well ...