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Citations for Millipore Sigma :
8051 - 8100 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... or without (non-inducing) 0.5% galactose in a microtiter plate sealed with an air-permeable membrane (Breathe-Easy; Sigma-Aldrich, St. Louis, MO) in n=6 replicates ...
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bioRxiv - Cell Biology 2021Quote: ... Precultures of 100 μl were inoculated at an OD600 of 0.1 in round bottom microtiter plates and sealed with an air-permeable membrane (Breathe-Easy; Sigma-Aldrich, St. Louis, MO). The growth curves started at OD600 0.1 and incubated at 30°C for 72 h under constant shaking ...
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bioRxiv - Cell Biology 2020Quote: Equal amounts of lysed proteins were separated by 7.5% or 10% SDS-polyacrylamide gels (under reducing conditions for CX3CR1 and non-reducing for total AAT or AAT polymer analysis) prior to transfer onto polyvinylidene difluoride (PVDF) membranes (Meck-Millipore, Burlington, MA, USA). Membranes were blocked for 1 h with 5% low fat milk (Carl Roth ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to nitrocellulose membranes and probed with NY2403 anti Lamp1 (see below) at 1:5000 and anti αTubulin (Sigma, T5168; 1:10,000) as loading control.
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bioRxiv - Cancer Biology 2020Quote: ... Equal volumes of protein samples were separated by SDS-poly acrylamide gel electrophoresis and electro-transferred to PVDF membranes (Millipore, Billerica, MA, USA). After blocked with 5% non-fat milk dissolved in TBST (10mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... The GCN4-M87/LL-37 complex was concentrated by ultrafiltration using a 3,500 MWCO membrane (Millipore; 4,500 x g, 30 min, 15 °C) to 8 mg/ml ...
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bioRxiv - Microbiology 2022Quote: ... GCN4-M87 alone was concentrated to 10 mg/ml by ultrafiltration through 3,500 MWCO membrane (Millipore; 4,500 x g, 30 min, 4 °C) The complex was brought to RT before introduction into crystallization drops to overcome its low solubility at 4 °C.
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bioRxiv - Plant Biology 2022Quote: ... normalizing protein levels to the Rubisco large subunit visualized in membranes stained with Ponceau (0.1 % Ponceau S Sigma-Aldrich in 5 % acetic acid). The statistical differences between the average protein levels of each sample were inferred using a Student’s t-test.
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bioRxiv - Microbiology 2022Quote: ... and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... taken at different points along the river width were filtered through a vacuum filtration system under sterile conditions using a membrane filter (pore size 0.22 μm; Millipore, GV CAT No GVWP04700). To prevent filter rupture ...
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bioRxiv - Biochemistry 2022Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, Cat No A2179) in PBST ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amount of lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membrane (Merck Millipore, Burlington, MA, USA) in 25 mmol/L Tris ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filter-sterilized (0.2 μm pore size PES membrane) then clarified supernatants were concentrated by ultrafiltration at 40°C using a Millipore stirred ultrafiltration apparatus (Millipore-Sigma; Burlington, MA). Concentrated supernatants were then ultracentrifuged at 100,000 xg for 2 hrs at 40°C to pellet crude OMVs ...
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β-catenin signaling via astrocyte-encoded TCF7L2 regulates neuronal excitability and social behaviorbioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with secondary antibodies (anti-Rabbit IgG – peroxidase antibody, Sigma Aldrich, A0545; anti-Mouse IgG – peroxidase antibody, Sigma Aldrich, A9044) for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... were pooled and filtered through a vac-uum system under sterile conditions using a membrane filter (pore size 0.22 μm; Millipore, GV CAT No GVWP04700). To prevent rupture ...
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bioRxiv - Physiology 2020Quote: ... were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). For western blotting ...
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bioRxiv - Molecular Biology 2022Quote: ... equal amounts of protein extracts were subjected to separation SDS-PAGE before being transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA), and subsequent visualization by Western blotting with distinct antibodies as indicated ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Equal amounts of protein (25-35 μg) from retina and choroid samples were resolved by 10% SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Burlington, MA, USA). The antibodies against PRMT5 (rabbit ...
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bioRxiv - Microbiology 2022Quote: ... were loaded into each well of a 4% to 12% polyacrylamide gradient gel to allow electrophoretic separation of gonococcal constituents (under denaturing conditions) and transfer to Immobilon-P membranes (Millipore Corporation, Bedford, MA). Membranes containing separated gonococcal constituents were then incubated overnight (4°C with rotation ...
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bioRxiv - Cancer Biology 2022Quote: ... was applied in 5-20% SDS-polyacrylamide gel (SuperSep Ace; Cat. No. 199-15191, Fujifilm-Wako chemical) and transferred to the PVDF membrane (Immobilon-P; Millipore, Bedford, MA, USA). The membrane was blocked by 5% skimmed milk or 5% BSA for 1 h with shaking ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Biochemistry 2020Quote: ... Precultures of 100 μl were inoculated at an OD600 of 0.1 in microtiter plates and sealed with an air-permeable membrane (Breathe-Easy; Sigma-Aldrich, St. Louis, MO). The growth curves started at OD600 0.1 and incubated at 30°C for 72 h under constant shaking ...
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bioRxiv - Biophysics 2020Quote: ... sonicated for 25 min in a cold water bath and filtered first through a 0.22 µm and then through a 30 kD centrifu-gal membrane filter (Millipore Sigma, UFC30GV and UFC5030) as described previously (25) ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA, USA). After blocked with 5% non-fat milk ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were separated through electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel and then transferred onto an Immobilon-P membrane (Merck Millipore, Burlington, MA, USA). After blocking with 5% bovine serum albumin ...
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bioRxiv - Molecular Biology 2020Quote: ... were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to Polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA). Membranes were then blocked for 90 min with specific 5% non-fat dried milk and incubated with primary detection antibodies ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... membranes were incubated overnight on gentle agitation at 4°C with primary antibodies (TH, 1:1000; GAPDH, Sigma, G8795, mouse monoclonal, 1:10,000) diluted in antibody buffer (1:1 of Tris buffered saline (TBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Physiology 2021Quote: ... Fresh 5μm-thick cryosections were treated for five minutes with 0.25% Triton X-100 in phosphate-buffered saline and incubated for 45 minutes at room temperature with a mouse monoclonal MHC1-antibody (A.4840; Developmental Studies Hybridoma Bank, Iowa City, IO) and with the basal membrane marker laminin (L9393; Sigma, Zwijndrecht, The Netherlands). Thereafter ...
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bioRxiv - Cancer Biology 2021Quote: ... Exosomes were collected by a size-based EV isolation method with modifications19,61 using 50-nm membrane filters (EMD Millipore, VMWP02500 or Whatman, WHA110603) with holders (EMD Millipore ...
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bioRxiv - Immunology 2021Quote: ... Each membrane was re-used to for the loading control vinculin (mouse-a-hVCL, clone hVIN-1, 116 kDa, Sigma-Aldrich, cat#V9131). As secondary antibodies ...
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bioRxiv - Physiology 2020Quote: ... After transfer PVDF membranes were washed with tris-buffered saline (TBS) and then blocked with 5% bovine serum albumin (BSA, Sigma-Aldrich, Missouri, USA) in 1X TBS with 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were transferred to PVDF membranes and subjected to immunoblotting with anti-HIV Ig polyclonal serum (NIH ARRRP) and anti-FLAG monoclonal antibody (Sigma-Aldrich, Clone M2). For immunoblotting of MLV particles ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were centrifuged for 7 minutes at 2000rpm for higher transfection efficiency and were incubated for 1-3 days with coving by Breathe-Easy sealing membrane only (Sigma-Aldrich, # Z380059-1PAK). All assays were performed on Day 1-3 following transfection ...
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bioRxiv - Biophysics 2023Quote: ... Stretching is performed using 2x2 cm flexible polydimethylsiloxane (PDMS) membranes (Sylgard184; crosslinker-to-base ratios of 1:14; cured for 24 h at 65°C, Sigma-Aldrich, St. Louis) as described in (45) ...
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bioRxiv - Neuroscience 2023Quote: ... The same procedure was then applied to the same membranes with anti-tubulin antibodies (mouse anti-tubulin, 1:40000, Sigma Aldrich cat. # T16199). The molecular weight ladder corresponds to Invitrogen SeeBlue™ Plus2 (reference LC5925).
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bioRxiv - Physiology 2023Quote: ... Equal amounts of protein extracts were subjected to electrophoresis in 8–12% SDS- polyacrylamide gels and then transferred to PVDF membranes (EMD Millipore, Burlintgon, MA). The membranes were blocked with 5% non-fat milk at room temperature and then incubated with relevant primary antibodies at 4°C overnight ...
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bioRxiv - Evolutionary Biology 2023Quote: ... to remove large particles and the filtrates were then filtered through 0.22 μm pore-size membrane filters (Pellicon cartridge, Millipore Corp., Billerica, MA, USA) to collect microbial cells ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated overnight with primary antibody (rabbit anti-REST1, Millipore CS200555, 1:1000, or mouse anti-GAPDH, Millipore MAB374, 1:500) in TBST with 5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2022Quote: ... blood mixture was offered in 4 cm diameter petri dishes containing a triple membrane of PARAFILM® M (Sigma-Aldrich, catalog #P7543, USA) on top of the cages at 37 ºC by using heat packs for one hour ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein samples were then separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by transfer to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). The membranes were first blocked with 5% skim milk for 1 hour and then incubated with the primary antibodies of SARS-CoV-2(2019-nCoV)Spike RBD Antibody (Sinobiology ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). The antibodies are listed in Supplementary Table 4 ...
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bioRxiv - Biophysics 2022Quote: ... The imidazole was removed from the purified protein using an Amicon Ultra centrifugal filter with a regenerated cellulose membrane (Millipore Sigma, Burlington, MA). At least 3 washes with imidazole-free PBS buffer were done ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 30 μL from each sample was subjected to electrophoresis on 8% or 15% polyacrylamide gel for ARAP1 and ADAP1 each and transferred into an Immobilon®-P transfer membrane (Merck Millipore Ltd., Germany). Membranes were blocked with 5% skim milk (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2024Quote: ... A housekeeping protein was also detected using the same blot by mild stripping and re-probing the membrane with an anti-GAPDH rabbit polyclonal antibody (Sigma-Aldrich, G9545-200).
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bioRxiv - Neuroscience 2024Quote: ... each sample was separated on 5-20% gradient e-PAGEL mini gels (ATTO Corporation, Tokyo, Japan) and transferred to polyvinylidene fluoride membranes (Merck Millipore, Billerica, MA, USA). The membranes were incubated for 30 min with blocking buffer (Nacalai Tesque) ...
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bioRxiv - Pathology 2024Quote: ... The membranes were incubated after washing with an anti-rabbit IgG alkaline phosphatase secondary antibody (1:5000, Sigma– Aldrich, St Louis, MO, USA) for 1 h ...
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bioRxiv - Physiology 2024Quote: ... the excess of reagents was eliminated by performing several washing steps with Milli-Q water using 4 mL cellulose membrane centrifugal filters (Amicon, MilliPore, 100 kDa, Merck). All MNP suspensions were sterilised by filtration using 0.22 μm MilliPore® filters before addition to cell cultures ...
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bioRxiv - Microbiology 2024Quote: ... Samples were dried using nitrogen and re-suspended in HPLC grade methanol and filtered through a 4mm PVFD membrane 0.45 μM filter unit (Cat. No. SLHV004SL, Millipore Sigma, St. Louis, MO) before analysis ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...