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Citations for Millipore Sigma :
7851 - 7900 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... host cells were transduced with viral particles in 6-well dish along with polybrene (hexadimethrine bromide; 8μg/ml) (Sigma-Aldrich) to increase the transduction efficiency ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by adding 100μl/well of Stop Reagent for TMB Substrate (450 nm, Sigma-Aldrich, Taufkirchen, Germany). Absorbance was measured at 450 nm ...
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bioRxiv - Cell Biology 2021Quote: ... GMPS were sorted as described above from CD45.1 and cells were cultured in a 12 well plate pre-coated with human fibronectin (EMD Millipore) in RPMI1640 media + 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells from each group of coated wells were incubated in the above media in the presence or absence of 150μM H2O2 (Sigma-Aldrich) for a further 24h ...
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bioRxiv - Cancer Biology 2021Quote: ... Viral vectors for shRNA expression as well as empty-cassette plasmids used as vector controls were obtained from the Mission TRC library (Sigma) via the McGill Platform for Cellular Perturbation (clone information and sequences in Supplementary Table 2) ...
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miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... 20 μL of the MTT solution was added to each well (5 mg/ml, Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). Then ...
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bioRxiv - Cell Biology 2020Quote: ... The 8 well dishes were plasma activated for 30 seconds and coated with either 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) in PBS or 20 μg/ml laminin (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... The 8 well dishes were plasma activated for 30 seconds and coated with either 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) in PBS or 20 μg/ml laminin (Merck Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Flag (M2) and anti-MAP2 (HM-2) antibodies as well as polyclonal rabbit anti-Flag antibodies (F3165, F7425) were from Sigma. Anti-Xpress antibodies were from Invitrogen (46-0528).
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bioRxiv - Cell Biology 2021Quote: SK-N-SH-N cells were plated onto 6-well tissue culture plates coated with poly-L-lysine (Sigma-Aldrich). Cells were plated at 3×105 cells/ml in MEMα medium containing a very low-serum level (2% FBS) ...
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bioRxiv - Neuroscience 2022Quote: The monolayer tightness in 12-well inserts was also assessed by measuring LY (Lucifer Yellow CH dilithium salt, Sigma-Aldrich) permeability as previously described (Molino et al. ...
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bioRxiv - Neuroscience 2022Quote: ... selective astrocytic toxin L-α-aminoadipate (L-AA) as well as Gabapentin were purchased from Sigma-Aldrich (St. Louis, MO). Caspase-1 siRNA was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Bioengineering 2022Quote: ... CD14+ monocytes were plated in wells of a 24-well tissue culture plate at a density of 150,000 monocytes per well and cultured in macrophage differentiation media consisting of RPMI-1640 (Sigma-Aldrich) supplemented with 10 % FBS ...
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bioRxiv - Molecular Biology 2022Quote: Collagen gels were made in a 24-well cell culture dish using a 1mg/mL collagen solution (Sigma, 08-115) and seeded with ~65,000 fibroblasts isolated from adult mouse hearts as described [20] ...
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bioRxiv - Microbiology 2022Quote: ... and seeded onto collagen IV-coated 96-well plates at the number of approximately ∼105 cells/well in the CMGF(+) or IntestiCult media supplemented with ROCK inhibitor Y-27632 (10 μM, Sigma) for 2 days.
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bioRxiv - Microbiology 2022Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Cell Biology 2022Quote: Freshly isolated MuSCs were plated in wells that were pre-coated with poly-D-lysine (0.1 mg/mL, EMD Millipore) and ECM (25 µg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Medium was removed from the wells and the cells were pre-treated with media containing NH4Cl (ammonium chloride) (Sigma-Aldrich) (2 ...
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bioRxiv - Microbiology 2022Quote: MDCK-SIAT1 cells were infected with the PR8 NP:WT/F346S viruses at an MOI of 5 TCID50/cell in a 6 well plate in the presence of 100µg/mL of cycloheximide (Sigma-Aldrich). Infected cells were harvested at 2 and 6hrs post-infection ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed as before and 100μl/well rabbit anti-mouse IgG1 or IgG2a conjugated to HRP (Sigma-Aldrich, Germany) diluted to 1 ...
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bioRxiv - Biophysics 2022Quote: ... The coverslips were subsequently placed on the 100X oil-immersion objective and a self-adhesive silicone culture well (Sigma Aldrich GBL103250 ...
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bioRxiv - Bioengineering 2022Quote: ... Twenty-thousand reporter cells were added to wells using 200 µL of DMEM media containing 2 µg/mL of doxycycline (Sigma) to induce Notch-Gal4 expression ...
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bioRxiv - Plant Biology 2022Quote: ... 30 seedlings of each genotype were transferred to 24 well-plates containing 1 ml 0.5X MS supplemented with 50 μM MG132 (474787, Sigma-Aldrich). Plates were sealed with surgical tape and placed on a shaker at 55 rpm for 16 h at 23°C under continuous light ...
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bioRxiv - Genomics 2022Quote: ... desired number of cells was resuspended in differentiation medium and 8*105 cells per well were seeded in a fibronectin-coated (10 □g/mL, Millipore) 6-well plate in differentiation medium ...
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bioRxiv - Neuroscience 2021Quote: ... The cell medium was aspirated from the wells and fresh medium with one of the factors (Bafilomycin, 200 nM, Merck Millipore; Rapamycin ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times by adding and removing 300 μl of wash buffer to each well (PBS + 0.05%Tween-20; Sigma, UK) and incubated for 1 hour with blocking buffer (Reagent Diluent ...
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bioRxiv - Neuroscience 2020Quote: Neurospheres were dissociated into single cells and 50.103 cells were plated onto 24-well Glass Slips (Millicell EZSLIDE, PEZGS0896) coated with 0.1mg/ml Poly-D-Lysine (Sigma-Aldrich), in culture medium without EGF/FGF2 for 1 h ...
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bioRxiv - Immunology 2020Quote: ... fixed antibodies were detected with horseradish peroxidase-conjugated goat anti-mouse IgA (100 µL / well - 1.5 µg/mL; Sigma-Aldrich) for 1h30 at 37°C and the reaction revealed with of 3,3’-5,5’-tetramethylbenzidine peroxidase substrate (100 µL/well - KPL ...
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bioRxiv - Immunology 2020Quote: PBMCs were rested overnight in complete medium and seeded at 2 × 105 cells/well in MultiScreen HTS Filter Plates (Merck Millipore) pre-coated with anti-IFN-γ (clone 1-D1K ...
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bioRxiv - Immunology 2020Quote: ... IELs were plated in 48-well plates and incubated at 37°C with 100ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma) and 200ng/mL ionomycin (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... coated glass slides in a 12-well plate O/N and then were stressed with 0.25 mM Sodium Arsenite (NaArs, S7400-100G Sigma-Aldrich) for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... the well content was transferred into a culture flask containing PYG medium supplemented with 20 μg/mL of G418 (Sigma) for two weeks ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells were seeded on 12-well plates with glass coverslips (Marienfeld Superior) coated with poly-L-lysine (Sigma-Aldrich) one day prior to transfection ...
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bioRxiv - Neuroscience 2021Quote: ... or glass coverslips in 24-well plates (for immunocytochemistry) coated with Poly-D-Lysine (5 μg/ml, PDL, A-003-E; Millipore) and laminin (1 mg/ml Sigma L2020) ...
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bioRxiv - Neuroscience 2021Quote: ... 1mL of HEK 293F cells from each batch was mixed together in a 12-well Corning Costar Not Treated Plate (Millipore) and incubated at 37°C with shaking on Orbi-Shaker (Benchmark ...
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bioRxiv - Neuroscience 2020Quote: ... Cortical neurons were plated in 6-well plates on coverslips coated with 100μg ml-1 poly-D-lysine (Sigma- Aldrich) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... the viral supernatant was harvested and used to transduce 25,000 mouse primary keratinocytes seeded in 48-well plates in the presence of 8 µg/ml polybrene (Sigma Aldrich). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... cells were approximately 80% confluent and passaged at 1:4 from 12-well plates to 15µg/mL Poly-L-Ornithine (Sigma-Aldrich) + 10µg/mL Laminin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The shRNA targeting the HPS-1 gene (TRCN0000292556) as well as the packaging plasmids pCMV-VSV-G and pCMV-dR8.91 were obtained from Sigma Aldrich. The plasmids encoding the shRNA sequences were pooled in equal concentrations and mixed with the packaging plasmids pCMV-VSV-G and pCMV-dR8.91 to generate lentiviral particles ...
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bioRxiv - Neuroscience 2021Quote: ... in either 4-well plates or P35 dishes (ThermoScientific) precoated for 4 h with 50 μg/ml poly-D-lysine (Sigma). Neurons were plated in DMEM containing 10 unit/mL penicillin ...
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bioRxiv - Neuroscience 2021Quote: ... and re-plated onto 13 mm glass cover slips in 24 well plates coated with 100 μg/ml Poly-L-Lysine (Sigma) and 1 μg/ml Laminin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... The plate was carefully transferred to 37°C incubator to allow the Matrigel to get solidified for 20 min following the addition 500 μL/well of pre-warmed media (DMEM advanced (Sigma), Wnt (25%) ...
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bioRxiv - Neuroscience 2021Quote: ... liquid media was added to the top of each well containing paralytic with DMSO and/or with 10uM MTZ (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... Experiments required the preparation of 24-well plates coated with a 1mg/ml poly-D-lysine hydrobromide (Sigma-Aldrich, P0899) solution for two hours and washed off with distilled water before cell plating ...
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bioRxiv - Microbiology 2022Quote: ... for 5 minutes at room temperature in individual wells of a 24-well tissue culture plate filled with 500 µL 20% sodium dodecyl sulfate or 20% sodium deoxycholate (Sigma). After disc placement ...
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bioRxiv - Microbiology 2022Quote: ... cells were replated in 6-well plates with RPMI medium containing 1mg/mL G-418 (Sigma-Aldrich, St. Louis, MO). As we described previously (52) ...
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bioRxiv - Microbiology 2022Quote: ... were seeded in a 24-well cell culture dish (pre-coated with 0.1 mg/mL poly-L-lysine (P9155-5MG, Sigma-Aldrich) for HEK-ACE2 ...
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bioRxiv - Microbiology 2022Quote: ... were seeded in a 24 well plate pre-coated with 0.1 mg/mL poly-L-lysine (P9155-5MG, Sigma-Aldrich) and 24 hours later used for transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated on 96-well plate in micro islands (35,000 cells/well) and maintained with dopamine neuron medium (DPM) containing 11.5 mM D-glucose (Sigma, G8769-100ML), 2 mM L-glutamine (Gibco ...
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bioRxiv - Immunology 2022Quote: ... TZM-bl cells were added to each well and incubated for 48 h at 37°C in presence with 12.5 μg/mL DEAE-Dextran (Sigma-Aldrich). Prior to data acquisition ...