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Citations for Millipore Sigma :
7851 - 7900 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Proteins were separated by 8.5% Sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Millipore, Immobilon-P, IPVH00010) using a semi-dry transfer unit (PEQLAB) ...
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bioRxiv - Cell Biology 2022Quote: ... 10% or 12% polyacrylamide gels before being transferred to PVDF Immobilon-FL membrane with pore size 0.45 μm (Millipore Billerica, MA, USA) using a Mini Trans-Blot electrophoretic transfer system (BioRad ...
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bioRxiv - Microbiology 2023Quote: Proteins in the cell and viral lysates (5 μg of total protein and 10 ng of p24 antigen) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat# IPVH00010). Membranes were blocked with 5% milk in PBS containing 0.1% Tween 20 (0.1% PBST ...
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bioRxiv - Microbiology 2023Quote: ... 700 µL supernatant was transferred to a new 1.5-mL tube and concentrated using Amicon Ultra 1 mL 10K membrane filters (Millipore, catalog number: UFC501096) to ≈40 µL by centrifuging at room temperature at 14,000 x g for ≈45 min ...
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bioRxiv - Microbiology 2023Quote: ... was added to the samples and were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore--Sigma, Marlborough, MA, USA). The membrane was blocked with Intercept® (PBS ...
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bioRxiv - Microbiology 2023Quote: ... was added to the samples and were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore--Sigma, Marlborough, MA, USA). The membrane was blocked with Intercept® (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Four small insertions were made and the retina was flat mounted on a filter paper annulus (1 mm inner radius; 0.8 µm hydrophilic MCE MF-MilliporeTM membrane filter, Merck Millipore Ltd., Tullagreen, Ireland). The retina was then mounted photoreceptor cell side up on a perforated 60-electrode MEA chip (60pMEA200/30iR-Ti ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... BDE-99 was dissolved in corn oil and filtered using a 0.22 μm Millipore Express Plus Membrane filter (EMD Millipore, Temecula, California). All mice used in this study were housed according to the Association for Assessment and Accreditation of Laboratory Animal Care International guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... The upper part of the membrane was incubated with a primary monoclonal mouse-anti-HA antibody (1:1000; H3663; Sigma-Aldrich, USA) to detect HA-tagged TRPM2 channel domain and the lower part of the membrane was incubated with rabbit-anti-FLAG (DYKDDDDK ...
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bioRxiv - Neuroscience 2023Quote: ... to 300 μm thickness and grown on Millipore Millicell inserts containing hydrophilic polytetrafluoroethylene (PTFE) membranes with 0.4 μm pores (#PICMORG50; Millipore Sigma, Burlington, Massachusetts) in 6-well dishes over media (1x MEM (Millipore-Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were separated by SDS-PAGE using 10% agarose gel and transferred to transfer membranes (Millipore-Sigma, St. Louis, MO, USA), which were incubated in 5% nonfat milk (A600669 ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were separated by SDS-PAGE using 10% agarose gel and transferred to transfer membranes (Millipore-Sigma, St. Louis, MO, USA), which were incubated in 5% nonfat milk (A600669 ...
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bioRxiv - Plant Biology 2023Quote: ... The flow-through was concentrated by syringe filtration using a 0.20-μm Micropore Express Plus membrane placed within a removable filtering syringe tip adapter (Millipore, Billerica, MA, U.S.A.). Filters were removed from the adapter and immediately frozen in LN2 until processing.
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Microbiology 2023Quote: ... skim milk/TBS and the membrane was washed 5x with 1x TBS before incubation with streptavidin-conjugated alkaline phosphatase (dilution 1:1,000, Sigma-Aldrich; Taufkirchen, DE) in 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... gentle peristaltic filtration (30 ml•min-1) was used to collect all cells and particles on a 0.22 μm Sterivex filter (PES membrane, Millipore, Burlington, MA, USA). Microbial biomass on the Steviex filter was used for 16S rRNA gene amplicon microbial community diversity analysis ...
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bioRxiv - Microbiology 2024Quote: ... 20-30 μg of protein for each sample was separated by 8-15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (Millipore, cat# IPVH00010) using Trans-Blot Systems (Bio-rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... the solution was diluted with warm PBS (40°C) and dialyzed (with 12-kDa molecular weight cut-off membrane; Sigma Aldrich, USA) against distilled water for 5-7 days at RT to remove excess methacrylic acid and other impurities ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then run on NuPAGE 4-12% Bis-Tris Protein Gels (Thermo #NP0335BOX) and transferred to a PVDF membrane (EMD Millipore #IPVH00005). Blots were probed with primary antibodies overnight at 4°C in 5% blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein samples were separated on ammediol SDS-PAGE [30] and transferred to a PVDF membrane (polyvinylidene difluoride, EMD Millipore, Burlington, MA, USA) in transfer buffer (10 mM N-cyclohexyl-3-aminopropanesulfonic acid ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were blocked with 5% skim milk in TBST and target proteins were detected with ANTI-FLAG® M2 antibody (Sigma-Aldrich) and anti-α-tubulin (SantaCruz).
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bioRxiv - Plant Biology 2022Quote: ... a drop of pollen germination media was covered with a 1×1cm piece of dialysis membrane (Sigma-Aldrich catalog number, D9652-100FT). Fresh ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant that contained the soluble fusion protein was filtered over Millex-GP syringe-driven 0.22-µm PES membranes (Millipore Sigma, Burlington, MD). The fusion protein was purified by fast protein liquid chromatography (FPLC ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing lentiviral particles was filtered through a 0.45 µM membrane (Fisher, 9720514) and was supplemented with 8 µg/µL polybrene (Sigma, TR-1003-G) prior to infection ...
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bioRxiv - Biochemistry 2024Quote: ... the insoluble fraction is removed by transferring TPP-PISA treated samples to a hydrophilic polytetrafluoroethylene 96-well filter plate with a 0.45 μm membrane pore size (MSRLN0410, Millipore, Burlington, Massachusetts, USA). The filter plate is pre-wetted with 100 μl of PBS and centrifuged for 2 minutes at 500xG ...
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bioRxiv - Biophysics 2024Quote: ... The phages were then purified with two different protocols: Protocol 1: Phage lysates were filtered through 0.22 µm polysulfone membrane filters (Merck Millipore, Billerica, MA, USA) and were used for subsequent purification and concentration by cross-flow filtration using Sartorius Hollow fiber cartridges ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... 20 µg proteins were separated by SDS-PAGE (10– 12% gels) and were blotted onto a nitrocellulose membrane (Millipore, Billerica, MA, USA). The membrane was blocked with 5% BSA in TBST at room temperature for 1h ...
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bioRxiv - Molecular Biology 2024Quote: ... the lysates were loaded onto a FuturePAGE™ 4–20% 15 Wells SDS page (ACE Biotechnology) and transferred to an Immobilon®-NC Transfer Membrane (Millipore). Blots were then blocked using Protein Free Rapid Blocking Buffer (Epizyme Biotech ...
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bioRxiv - Microbiology 2024Quote: ... while urine samples were concentrated through a polystyrene membrane in an Amicon ultracentrifugation system with a 100kDa cutoff (Millipore, Billerica, MA, USA) and then centrifuged at 15,000×g ...
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bioRxiv - Cancer Biology 2023Quote: ... Fifty micrograms of the respective samples were separated on 13% SDS-PAGE gel and transferred to an Immobilon®-P PVDF (polyvinylidene difluoride) membrane (Merk Millipore, IPVH00010) using XCell II™ Blot Module (Thermo Fisher Scientific ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... SDS samples containing 4∼8 μg total proteins were separated with 12% HEPES-Tris PAGE gel (MEILUNBIO, MA0243) and transferred to polyvinylidene difluoride membranes (Millipore, 70584-3). The membrane was blocked with 5% skim milk in TBST buffer (20mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The prepared proteins were separated by SDS-PAGE gel electrophoresis (Yazyme, Shanghai, China) and transferred to activated PVDF membranes (Millipore, MA, USA). The PVDF membranes were then gently rinsed with TBST and placed in 7% skim milk powder solution and closed on a shaker at room temperature for 3 h ...
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bioRxiv - Pathology 2023Quote: ... An equal amount of protein (10 µg) was subsequently separated by 12% SDS-PAGE and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, Bedford, MA). The membrane was blocked with a 5% skimmed milk solution prepared in Tris-buffered saline containing 0.1% Tween-20 (TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... was used following manufacturer’s instructions to perform a semi-wet transfer onto a 0.45 µm Amersham™ Protran® nitrocellulose membrane (Sigma, Cat# GE10600002). For blocking ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Transwell inserts were fixed with 100% methanol and the membranes were mounted on microscope slides using a medium containing DAPI (Fluoroshield, Sigma-Aldrich, USA). 10 images from different regions of the membrane were acquired with a fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera).
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bioRxiv - Developmental Biology 2023Quote: ... and blotting on a polyvinylidene difluoride (PVDF) membrane probed with rabbit monoclonal anti-histone H3 (Cat# 07690, EMD Millipore, 1:25000-50000), mouse monoclonal anti-alpha tubulin (Cat# NB100-690 ...
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bioRxiv - Microbiology 2023Quote: ... Other part of filtrate was filtered through a 0.22-μm-pore-size polycarbonate membrane (45 mm diameter; Millipore, Bedford, MA, United States) to obtain the fractions water containing viruses ...
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bioRxiv - Microbiology 2023Quote: ... Corresponding water samples were taken by filtering 2-L of tank water through 0.22-μm pore-size cellulose acetate membranes (Millipore Sigma, Burlington, MA). Samples were stored at −80°C until processing.
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bioRxiv - Microbiology 2023Quote: ... Each plate was inoculated with 100 μl per well of the respective prepared solution and then sealed using Breathe-Easy® sealing membranes (Sigma-Aldrich). Plates were then transferred to a Biostack Microplate Stacker (Agilent ...
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bioRxiv - Bioengineering 2024Quote: ... Amicon Ultra-0.5 (PLBC Ultracel-3 membrane, 3 kDa) and Amicon® Ultra-15 Centrifugal Filters (3 kDa MWCO) were from Millipore (Tokyo, Japan). Calcofluor-white and imidazole were from Sigma-Aldrich (Tokyo ...
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bioRxiv - Neuroscience 2023Quote: ... and 40 μg of lysates were loaded onto 10% Tris/glycine PAGE gels followed by transfer onto PVDF membranes (EMD Millipore, # IPFL00010) at 30V for 1hr) ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was incubated 2h at 4°C with ANTI-FLAG antibodies® M2-peroxydase (Sigma-Aldrich, used at 1/ 1000 dilution). The antibodies were detected by using Lumi-Light Western Blotting Substrate (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... and then clarified via passing through Millex® -GP Fast Flow & Low Binding Millipore Express® PES Membrane 0.22 µm syringe filter unit (SLGP033RS, Merck Millipore, USA).
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bioRxiv - Microbiology 2024Quote: Approximately equal amounts of GST fusion proteins and alanine-mutated GST fusion proteins were separated by 12% SDS-PAGE and transferred to PVDF transfer membranes (Millipore, MA, USA). The membranes were blocked with 5% skimmed milk powder for 2 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Cell-free extracts were then resolved by SDS-PAGE using 12.5%-15% polyacrylamide gels and subsequently electro-blotted onto a 0.45 μm polyvinylidene difluoride (PVDF) Immobilon®-P membrane (Merck Millipore, Sigma Aldrich) at 100 V for 90 minutes ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked in PBS-T or TBS-T complemented with 5 % (w/v) bovine serum albumin (BSA, Sigma-Aldrich, A3294-50G) for up to 3 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... electrophoresed through 10% acrylamide (Nacalai Tesque) or 10−20% gradient gels (ATTO, Tokyo, Japan) and blotted onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA). PVDF membranes were detected using anti-hDectin-1 polyclonal antibodies (AF1859 ...
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bioRxiv - Developmental Biology 2022Quote: ... and aliquots of 15 μg protein were separated by SDS-PAGE and blotted onto a polyvinylidene fluoride (PVDF) membrane (Millipore, MA, USA). Membranes were blocked at room temperature for 15 min in QuickBlock™ Western (Beyotime) ...