Labshake search
Citations for Millipore Sigma :
7801 - 7850 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Protein samples were separated in 4-15% polyacrylamide gels and transferred to 0.2 µm Immobilion-PSQ transfer membrane (Millipore, Cat. No ISEQ00010) using a Bio-Rad transfer system ...
-
bioRxiv - Cell Biology 2021Quote: ... About 20 ug total proteins in each sample were fractionated by 10% SDS PAGE gel and transferred to PVDF membranes (Millipore Corporation, USA) and immunoblotted with primary antibody anti-GFP (Invitrogen-Molecular Probes ...
-
bioRxiv - Cancer Biology 2021Quote: Proteins were separated using a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels and transferred onto Immobilon-FL PVDF membrane (Millipore). The membranes were blocked in 5% BSA for 1 hour ...
-
bioRxiv - Cell Biology 2021Quote: ... The proteins of tissue samples were resolved by 10% SDS-PAGE and transferred to the PVDF membrane (0.2 µm pore size, Millipore, Billerica, MA, USA). The membranes were immunoblotted with primary antibodies overnight at 4°C followed by secondary antibody incubation for 2 hours ...
-
bioRxiv - Cancer Biology 2021Quote: ... 15 μg of tumor protein was resolved on 12% SDS-PAGE gels and transferred to Immobilon-FL PVDF membrane (Millipore, catalog# IPFL00010). Membranes were blocked with 5% (w/v ...
-
bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10,000; anti-rabbit, Sigma-Aldrich A0545 or anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
-
bioRxiv - Cell Biology 2022Quote: ... the SDS samples were applied onto an Amicon filter unit (MWCO = 10 kDa; Ultracel-10 cellulose membrane, Cat.No; UFC201024, Merck Millipore, Billerica, MA, USA) to trap the proteins ...
-
bioRxiv - Cell Biology 2022Quote: ... the proteins were wet transferred onto ‘Amersham™ Protran™0.2 µm NC’ nitrocellulose membranes (GE10600002 Sigma-Aldrich Handels Gmbh, Vienna, Austria) at constant 80 V for 1,5 h ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant containing lentiviral particles was filtered through 0.45 µM membrane (Fisher, 9720514) and was supplemented with 8 µg/µL polybrene (Sigma, TR-1003-G) prior to infection ...
-
bioRxiv - Immunology 2022Quote: ... The inner surface of Transwell inserts (3.0 μm pore transparent polyester membrane) pre-coated with 100 μl of human collagen IV solution (34 μg/ml; Sigma-Aldrich, USA) were seeded with 100 μl of an enteroid fragment suspension ...
-
bioRxiv - Molecular Biology 2022Quote: RPA and RT-RPA reactions are performed on a variety of commercially available porous membranes (Millipore GF041, Whatman GF/DVA, Whatman Fusion 5, and Millipore PES GPWP04700). We first cut the membranes with a flatbed cutter (FCX4000-50ES ...
-
bioRxiv - Immunology 2022Quote: ... A 15 ul of lysate was resolved on 12% SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, MA). The membrane was blocked in 5% powder milk overnight in 4°C ...
-
bioRxiv - Pathology 2022Quote: ... Twenty micrograms of protein per lane were loaded onto an 8-12% polyacrylamide Gel and then transferred to PVDF membranes using an electrophoresis chamber (Millipore, Shanghai, China) for 1.5 h ...
-
bioRxiv - Microbiology 2022Quote: ... The samples with crystals were dissolved in 5 volumes of molecular grade water to dissolve all precipitated salt and then filtered through a hydrophilic polycarbonate membrane with a pore size of 0.2 μm (Millipore Sigma, Cat.: GTTP04700) using a glass filtration apparatus ...
-
bioRxiv - Bioengineering 2022Quote: ... and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (10%) and then transferred onto poly vinylidene fluoride membrane (EMD Millipore, MA, USA). The membranes were blocked in phosphate-buffered saline containing 5% fat-free milk for 2 h ...
-
bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated with anti-TSP1 (1:500, NeoMarkers catalog#MS-1066-P1) or anti-βactin (1:50000, Sigma-Aldrich catalog#A3854). Appropriate peroxidase-conjugated secondary antibodies (1:10000 ...
-
bioRxiv - Microbiology 2020Quote: ... The supernatant was filter-sterilized (0.2 μm pore size PES membrane) then clarified supernatants were concentrated by ultrafiltration at 40°C using a Millipore stirred ultrafiltration apparatus (Millipore-Sigma; Burlington, MA). Concentrated supernatants were then ultracentrifuged at 100,000 xg for 2 hrs at 40°C to pellet crude OMVs ...
-
bioRxiv - Developmental Biology 2021Quote: ... the membrane was then incubated overnight at 4°C with primary antibody 1:12000 mouse anti-α-Tubulin antibody (SIGMA, Cat. # T5168). Followed by 1h incubation with secondary antibody ...
-
bioRxiv - Molecular Biology 2021Quote: ... each sample with equal amount of protein was subjected to SDS-PAGE gel (10% or 15%) and transferred to a PVDF membrane (Millipore, Massachusetts, USA). The membrane was incubated with the specific primary antibody overnight at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... and HA-tagged proteins of samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to a PVDF membrane (Merck Millipore, Tokyo, Japan). The membrane was incubated with rabbit anti-N antiserum or mouse anti-GFP antibody ...
-
bioRxiv - Plant Biology 2021Quote: ... The dried extracts were dissolved in 350 μl purified water and filtered through MultiScreen PCR-96 Filter Plate membranes (Merck Millipore, Darmstadt, Germany) to remove high-molecular-mass compounds ...
-
bioRxiv - Cancer Biology 2020Quote: ... Gels were blotted onto nitrocellulose membranes and probed for each antigen using the following antibodies at the indicated dilutions: Human PEAK1 (Millipore 1:400), Mouse PEAK1 (Abgent 1:400) ...
-
bioRxiv - Microbiology 2022Quote: ... 5°6.7’ E) in September and October 2017 by filtering 2 L of seawater through PVDF membrane filters (Merck Millipore, Burlington, Massachusetts,USA), pore size ...
-
bioRxiv - Systems Biology 2020Quote: ... separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride membrane (PVDF, Millipore, Bedford, MA, USA). The PVDF membrane was blocked with 5% milk in Tris-buffered saline with Tween-20 (TBST ...
-
bioRxiv - Cell Biology 2020Quote: ... The pooled fractions were transferred to ice-cold PBS pre-rinsed Amicon Ultra-4 centrifugal filter units with Ultracel-30 membrane (Merck Millipore, Massachusetts, USA), then centrifuged with 3,200 x g at 4 °C for 10 min ...
-
bioRxiv - Plant Biology 2020Quote: ... The dissolved samples were filtered through 13-mm diameter Millex filters with a 0.22-μm pore size nylon membrane (Millipore, Bedford, MA, USA). Filtered extract (10 μl ...
-
bioRxiv - Pathology 2021Quote: ... 20 µg of total protein was separated by electrophoresis in a 10% SDS polyacrylamide gel and transferred to a PVDF membrane (Millipore, Billerica, MA) for western blot analysis.
-
bioRxiv - Microbiology 2020Quote: ... The total protein present in another half of supernatant was precipitated by using ammonium sulfate (80%) and dialysis using membrane tubing 2 kDa molecular weight cut-off (MWCO) (Sigma-Aldrich, India).
-
bioRxiv - Immunology 2020Quote: ... Cultured cell lysates (30 μg) were electrophoresed in an 8–12% SDS-PAGE gel and transferred to a PVDF membrane (Millipore, MA, USA). PVDF membranes were blocked with 5% skim milk in phosphate buffered saline with 0.1% Tween 20 (PBST ...
-
bioRxiv - Systems Biology 2020Quote: ... Resolved proteins were transferred to nitrocellulose membranes and detected by standard Western blotting using monoclonal anti-FLAG M2-mouse primary antibody (Sigma Aldrich, F1804) and HRP labelled anti-mouse IgG secondary antibody (Cell Signalling technology ...
-
bioRxiv - Biochemistry 2020Quote: ... The resulting cell free supernatant (CFS) was concentrated through a 10 kDa polyethersulfone membrane disc using an Amicon® Stirred Cell (Merck Millipore, Germany) to a final volume of 50 mL ...
-
bioRxiv - Bioengineering 2020Quote: ... the cells migrated through the membrane to the lower layer of each well were counted by H33342 staining (Sigma, St Louise, USA).
-
bioRxiv - Cell Biology 2020Quote: ... The membrane was probed against the endogenous proteins by incubation with primary Rabbit anti-myosin VI (1:500, Atlas-Sigma HPA0354863-100UL) or Mouse anti-RNAPII phospho Ser5 (1:500 ...
-
bioRxiv - Physiology 2020Quote: ... cell debris and sperm were removed from intact seminal plasma by centrifugation and > 220 nm vesicles were filtered with a 0.22 μm MF-Millipore™ membrane (Millipore, MA, USA). The filtered seminal plasma was centrifuged at 68,000 g for 90 min and regarded as exosome-free seminal plasma ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... of infected cells were separated on 10% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Sigma, Burlington, MA, USA). After being blocked with 3% BSA in TBS buffer containing 0.05% Tween 20 ...
-
bioRxiv - Biophysics 2020Quote: ... The otolithic membrane was gently removed after 8-min enzymatic dissociation with 50 μg/mL Collagenase IV (Sigma Aldrich, St. Louis, MO). For experiments in which calcium concentration of endolymph was varied ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... the AgNP suspension was washed with deionized (DI) water by ultrafiltration through a membrane with pore size of 3 kDa (Amicon Ultracel 3 KDa Ultrafiltration Discs, Millipore, Billerica, USA) under nitrogen gas pressure (1 bar ...
-
bioRxiv - Biochemistry 2021Quote: ... were heat-denatured in loading buffer and separated via 10% gel electrophoresis (SDS-PAGE) followed by transferred onto PVDF membrane (Millipore, Germany, IPVH00010). Then the membrane was blocked with 5% skim milk-TBST solution (20 mM Tris-HCl (pH 7.6) ...
-
bioRxiv - Cancer Biology 2021Quote: ... we used a Boyden chamber which consists of cell culture insert containing 8-μm pore polyethylene terephthalate membrane (EMD Millipore, Burlington, MA). The inserts were seated in 24-well companion plates ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... Approximately 40 μg of protein was separated on 12% SDS-polyacrylamide gel (SDS-PAGE) and the gel was transferred to polyvinylidene difluoride (PVDF) membrane (Millipore, MIT, USA). The membrane was blocked with 5% nonfat dry milk in TBST (Trisbuffered saline/0.1% Tween-20 ...
-
bioRxiv - Cell Biology 2021Quote: ... 8% SDS-PAGE was used for protein separation by electrophoresis and transferred to a PVDF membrane (Immobilon®-P, Millipore Corporation, IPVH00010). 10% BSA was used to block the membrane followed by protein detection using primary antibody of 1:1000 dilution such as anti-β actin (C4 ...
-
bioRxiv - Neuroscience 2021Quote: ... were separated by 7,5% or 10% SDS-polyacrylamide gel (SDS-PAGE) electrophoresis and transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore Corporation, USA). Western blot analysis was performed with the following primary antibodies ...
-
bioRxiv - Bioengineering 2021Quote: ... EVs were collected by a size-based EV isolation method [35, 36] using 50 nm membrane filter (Whatman, WHA110603) with holders (EMD Millipore, SX0002500). Holders with 50 nm membrane filters were connected to a vacuum manifold (Qiagen ...
-
bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were probed with anti-mouse-AID (1/25, eBioscience, 14-5959-82) and anti-mouse-tubulin (1/5000, Sigma-Aldrich, T9026). Then ...
-
bioRxiv - Bioengineering 2020Quote: ... and buffer-exchanged with phosphate-buffered saline (PBS) using an Amicon Ultra centrifugal filter unit with a 10 kDa cutoff membrane (Millipore, Burlington, MA). To determine the protein concentration ...
-
bioRxiv - Microbiology 2021Quote: ... Each water sample collected (~ 10 L) was immediately filtered through a 0.22 μm polycarbonate membrane (diameter 45 mm; Millipore, Bedford, MA, USA) using a peristaltic pump ...
-
bioRxiv - Microbiology 2020Quote: ... One hundred mL of sewage samples were filtered with a 0.22-μm-pore-size mixed cellulose ester membrane (Merck Millipore, Billerica, MA, USA). DNA was extracted from the filtered membranes and from 150–200mg of the nonhuman fecal samples using the ZR Fecal DNA MiniPrep™ Kit (Zymo Research ...
-
bioRxiv - Immunology 2021Quote: ... Lysates were separated by SDS-PAGE using Tris-Glycine gels loaded with about 1×106 cell equivalents per well and transferred onto PDVF membrane (Millipore, Burlington, MA). Blots were blocked in either 5% Milk (Nestle ...
-
bioRxiv - Genomics 2021Quote: ... Total protein (40 μ g) was electrophoresed on a 10% polyacrylamide gel by SDS-PAGE and transferred to PVDF membranes (Millipore, USA), followed by blocking with 5% milk for 2 h ...