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Citations for Millipore Sigma :
7651 - 7700 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... biofilm supernatants (either single or dual-species biofilms grown for 72 hrs) were recovered and filter sterilized using 0.2-micron polyether sulfone membrane filters (Millipore, Massachusetts, USA), then mixed at equal ratio (v/v ...
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bioRxiv - Microbiology 2024Quote: ... Cell lysates were subjected to SDS-polyacrylamide gel electrophoresis (PAGE) then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). Proteins were incubated with primary antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was filtered through a hydrophilic polytetrafluoroethylene membrane filter (Millex-LG, 0.2 um × 4 mm, Merck Millipore, Darmstadt, Germany) and transferred to a screw-top glass vial ...
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bioRxiv - Biochemistry 2024Quote: ... of the three cell lines were separated in a 12% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a polyvinylidene difluoride membrane (PVDF) (EMD Millipore) at 100V for 1h ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were equilibrated in pre-chilled transfer buffer with 15% methanol and then protein was transferred to a 0.45 µm nitrocellulose membrane (Sigma catalog# 10600002) via wet electroblotting on ice for 1 h at 300 mV ...
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bioRxiv - Molecular Biology 2024Quote: ... and the membrane blocked with 5% skim milk powder dissolved in PBS-T (1x PBS with 0.1% Tween-20 (Sigma-Aldrich #P1379)) for ∼1 h at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Microtiter plates were incubated at 30 °C for the duration of the time course at “high” shake speed setting and linear shaking mode in a 96 well microtiter plate sealed with a Breathe-Easylll transparent membrane (Sigma-Aldrich). Three biological replicates were processed by growthcurver R package and doubling times were found for all strains (Sprouffske and Wagner 2016).
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bioRxiv - Immunology 2024Quote: ... Proteins from cell lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride membranes (Millipore IPVH00010). For native blots ...
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bioRxiv - Microbiology 2024Quote: ... Equivalent amounts of total protein were loaded into a 12% SDS-PAGE for electrophoresis and then transferred into a PVDF membrane (Millipore, USA). After blocking nonspecific binding with 5% skimmed milk in TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 μL of each sample was loaded onto a 12% SDS-PAGE gel for electrophoresis and transferred to Immobilon-FL PVDF membrane (Millipore, IPFL00010) using a Power-Blotter Semi-dry transfer system (ThermoFisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 30 μg of protein from each sample were electrophoresed on a 4-12% SDS gradient gels and transferred to polyvinylidene difluoride membranes (Nitrocellulose; Millipore Corp). 1% BSA was used as blocking buffer for all phosphate proteins ...
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bioRxiv - Neuroscience 2024Quote: Protein samples were separated using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Millipore, IPVH00010). The membranes were then blocked with 5% skimmed milk (BBI ...
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bioRxiv - Physiology 2024Quote: ... To better visualize the inner membrane structures ultrathin cryosections were contrasted and stabilized with a mixture of 3% [w/v] tungstosilicic acid hydrate (Sigma-Aldrich) and 2.5% [w/v] polyvinyl alcohol (Sigma-Aldrich)47 ...
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bioRxiv - Biophysics 2024Quote: ... A syringe was prepared by stopping it with an end cap and a Whatman polycarbonate membrane filter with 10μm pore size (Sigma-Aldrich, USA) was cut to fit in the bottom to prevent the Sepharose beads from coming out ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of total proteins were separated using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... swab samples in VTM were centrifuged at 3200 RPM for 15 minutes and the resulting liquid was passed through a 0.22 µm filter (Membrane Solutions, USA) and then the filtrate was treated with a mix of nucleases: Benzonase (Sigma-Aldrich, USA), Turbo DNAse ...
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bioRxiv - Physiology 2024Quote: ... transporters on the apical membrane of blood-brain barrier cells.36 We used the dye Rhodamine B (Rho B) (Sigma R6626) to probe transcellular permeability ...
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Enhancing efficacy of the MEK inhibitor trametinib with paclitaxel in KRAS-mutated colorectal cancerbioRxiv - Cancer Biology 2024Quote: ... Cell lysates were prepared in a radioimmunoprecipitation assay buffer with protease and phosphatase inhibitors as described previously.32 The extracted proteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis following a standard protocol and transferred to Immobilon polyvinylidene membranes (EMD Millipore). For 1 hour ...
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bioRxiv - Physiology 2024Quote: ... All images were normalized to total protein from the same corresponding membrane using Amido Black total protein stain (Cat. No. A8181) (Millipore Sigma).
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bioRxiv - Bioengineering 2024Quote: ... Microbubble production was started by rehydrating the lipid film in 5 mL PBS which was saturated with C4F10 (F2 Chemicals) and addition of 125 µL BioTracker 400 Blue Cytoplasmic Membrane Dye (SCT109, Sigma-Aldrich) to fluorescently label the microbubble coating ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted fraction was concentrated using an ultrafiltration centrifugal membrane unit (Amicon Ultra 15 MWCO 10 kDa; Millipore, Billerica, MA, USA) and loaded onto a gel filtration chromatography column (HiLoad 16/60 Superdex 200 pg ...
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bioRxiv - Biophysics 2024Quote: ... The end was stopped with an end cap and a Whatman polycarbonate membrane filter of 10µm pore size (Sigma-Aldrich, USA) was cut and placed in the bottom of the syringe to prevent the Sepharose from running out ...
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bioRxiv - Cell Biology 2024Quote: ... The delipidated fetal bovine serum (dFBS) was sterilized by filtration with a 0.22 µm membrane (Millex-GV, Millipore S.A., Molsheim, France).
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bioRxiv - Bioengineering 2024Quote: The preparation of the Na+-selective membrane cocktail was conducted using a composition that included Na ionophore X (1% w/w, Sigma-Aldrich), sodium tetrakis[3,5-bis(trifluoromethyl)phenyl] borate (Na-TFPB ...
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bioRxiv - Plant Biology 2024Quote: ... Nylon membranes used in this work had a porosity of 0.45 μM and were 47 mm in diameter (Sigma-Aldrich, Z290807). Prior to imaging ...
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bioRxiv - Developmental Biology 2024Quote: ... 10-30 μg total protein was loaded for each sample and separated by 10% SDS-PAGE gel and transferred to PVDF membranes (Millipore; GVHP29325). Then ...
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bioRxiv - Biophysics 2024Quote: ... 500 ng of BiP were separated via SDS-PAGE on 10 % polyacrylamide gels (homemade) and transferred on Immobilon®-P PVDF membranes (Merck-Millipore) for 1.5 h at 230 mA using a Biometra Fastblot B44 (Analytik Jena ...
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bioRxiv - Biochemistry 2024Quote: ... and the samples were then filtered through a 0.1μm polyvinyldene fluoride membrane spin-filter (Amicon, Merck Millipore Cooperation, Billerica, MA, USA) before being transferred to liquid chromatography vials for analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amount of proteins (30 µg) were resolved on 10% SDS-PAGE and then transferred onto polyvinylidene fluoride membranes (EMD Millipore). Nonspecific antibody binding sites were blocked in 5% nonfat milk in TBS with Tween-20 (TBST ...
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bioRxiv - Biochemistry 2024Quote: ... A total of 20 µg was separated on 10 or 15% SDS-PAGE gels and then transferred onto a PVDF membrane 20 or 45 μm (Millipore, Inc.). After blocking with 5% Difco Skim Milk (BD Pharmingen ...
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bioRxiv - Cancer Biology 2024Quote: ... 50μg of each protein sample was run on a 10% SDS-PAGE gel followed with a 90V transfer at 4 °C for 90 minutes to a PVDF membrane (Millipore #IPFL00010). Membranes were blocked for one hour with Intercept PBS blocking buffer (LiCor #927-40000 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 μg of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Merk-Millipore IPFL00010). Next ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Proteins were loaded on PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Samples were heated at 100 °C for 10 min in a 4X loading buffer ...
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bioRxiv - Immunology 2021Quote: ... Arginase activity in the CECs or splenocytes lysates and cell supernatant was determined using Arginase Activity Assay (Sigma) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Plaque assays were undertaken in vero E6 cells using a 1.2% (w/v) microcrystalline cellulose overlay (Sigma-Aldrich). Cytokines/inhibitors were used at the following concentrations ...
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bioRxiv - Molecular Biology 2020Quote: Annexin V-FITC apoptosis assay was carried out as described in manufacturer’s protocol (APOAF, Sigma. St. Louis, MO). After transfected with Npac RNAi or control RNAi plasmid in 6-well dishes and selected for 4 days ...
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bioRxiv - Immunology 2021Quote: ... Then the medium was collected and replaced with stimulation medium (basal assay medium containing 100 µM histamine, Sigma) for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... The assay was developed by addition of o-phenylenediamine dihydrochloride (OPD, Cat n. 34006, Sigma Aldrich, Gillingham, UK) for 10 minutes and reactions were stopped with 2 M H2SO4 ...
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bioRxiv - Bioengineering 2020Quote: ... GAG was quantified using a modified dimethyl methylene blue (DMMB) assay [42] with Shark chondroitin sulfate (Sigma, C4348) as a standard ...
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bioRxiv - Biochemistry 2021Quote: GFAT’s amidohydrolysis activity was measured with a coupled enzymatic assay using bovine glutamate dehydrogenase (GDH, Sigma-Aldrich G2626) in 96 well standard microplates (F-bottom ...
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bioRxiv - Biochemistry 2020Quote: GFAT’s amidohydrolysis activity was measured with a coupled enzymatic assay using bovine glutamate dehydrogenase (GDH, Sigma Aldrich G2626) in 96 well standard microplates (F-bottom ...
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bioRxiv - Plant Biology 2021Quote: ... The stress assays were performed on CM plates (Holliday 1974) supplemented with 750 µM calcufluor white (Sigma-Aldrich), 3 mM hydrogen peroxide (H2O2) ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed a final time and the assay was developed using the SigmaFast pNpp reagent (Sigma Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... and for flippase specific assays in the presence of an ATP regeneration system (0.5 mM Phosphoenolpyruvate (Sigma-Aldrich) and 100 U ml−1 pyruvate kinase (Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2021Quote: ... Measurement of IDH activity on the enriched mitochondrial fractions was conducted using an IDH activity assay (Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Cell pellets were thawed on ice before adding 50-100μl radio immunoprecipitation assay (RIPA) buffer (Sigma, Dorset, UK) with protease and phosphatase inhibitors and lysed for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed beads were resuspended in 25 μl of kinase assay buffer with 100 μM ATP (A7699, Sigma) supplemented with phosphatase inhibitors and incubated for 20 min in an Eppendorf Thermomixer at 30°C at maximal mixing velocity ...
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bioRxiv - Bioengineering 2022Quote: ... Finally, the activity assay of immobilised GalT consisted of 0.16 mM UDP Gal (Merck, 16mM GlcNAc (Sigma-Aldrich), 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: Cell viability was evaluated using the 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (Sigma-Aldrich), which measures mitochondrial activity in living cells ...